Artikel ; Online: Effects of currently used pesticides and their mixtures on the function of thyroid hormone and aryl hydrocarbon receptor in cell culture.
Toxicology and applied pharmacology
2015 Band 284, Heft 3, Seite(n) 292–303
Abstract: Evidence suggest that exposure to pesticides can interfere with the endocrine system by multiple mechanisms. The endocrine disrupting potential of currently used pesticides in Denmark was analyzed as single compounds and in an equimolar mixture of 5 ... ...
| Abstract | Evidence suggest that exposure to pesticides can interfere with the endocrine system by multiple mechanisms. The endocrine disrupting potential of currently used pesticides in Denmark was analyzed as single compounds and in an equimolar mixture of 5 selected pesticides. The pesticides were previously analyzed for effects on the function of estrogen and androgen receptors, the aromatase enzyme and steroidogenesis in vitro. In this study, the effect on thyroid hormone (TH) function and aryl hydrocarbon receptor (AhR) transactivity was assessed using GH3 cell proliferation assay (T-screen) and AhR responsive luciferase reporter gene bioassay, respectively. Thirteen pesticides were analyzed as follows: 2-methyl-4-chlorophenoxyacetic acid, terbuthylazine, iodosulfuron-methyl-sodium, mesosulfuron-methyl, metsulfuron-methyl, chlormequat chloride, bitertanol, propiconazole, prothioconazole, mancozeb and its metabolite ethylene thiourea, cypermethrin, tau-fluvalinate, and malathion (currently banned in DK). In the T-screen, prothioconazole, malathion, tau-fluvalinate, cypermethrin, terbuthylazine and mancozeb significantly stimulated and bitertanol and propiconazole slightly reduced the GH3 cell proliferation. In the presence of triiodothyronine (T3), prothioconazole, tau-fluvalinate, propiconazole, cypermethrin and bitertanol significantly antagonized the T3-induced GH3 cell proliferation. Eleven of the tested pesticides agonized the AhR function, and bitertanol and prothioconazole inhibited the basal AhR activity. Bitertanol, propiconazole, prothioconazole and cypermethrin antagonized the TCDD-induced AhR transactivation at the highest tested concentration. The 5-component mixture had inducing effect but the combined effect could not be predicted due to the presence of bitertanol eliciting inhibitory effect. Upon removal of bitertanol from the mixture, the remaining four pesticides acted additively. In conclusion, our data suggest that pesticides currently used in Denmark can interfere with TH signaling and AhR function in vitro and might have the potential to cause endocrine disruption. |
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| Mesh-Begriff(e) | Animals ; Cell Line, Tumor ; Cell Proliferation ; Denmark ; Dose-Response Relationship, Drug ; Endocrine Disruptors/toxicity ; Genes, Reporter ; Liver/drug effects ; Liver/metabolism ; Mice ; Pesticides/toxicity ; Rats ; Receptors, Aryl Hydrocarbon/drug effects ; Receptors, Aryl Hydrocarbon/genetics ; Receptors, Aryl Hydrocarbon/metabolism ; Risk Assessment ; Signal Transduction/drug effects ; Thyroid Gland/drug effects ; Thyroid Gland/metabolism ; Thyroid Gland/pathology ; Transfection ; Triiodothyronine/metabolism | |||||
| Chemische Substanzen | Endocrine Disruptors ; Pesticides ; Receptors, Aryl Hydrocarbon ; Triiodothyronine (06LU7C9H1V) | |||||
| Sprache | Englisch | |||||
| Erscheinungsdatum | 2015-05-01 | |||||
| Erscheinungsland | United States | |||||
| Dokumenttyp | Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't | |||||
| ZDB-ID | 204477-8 | |||||
| ISSN | 1096-0333 ; 0041-008X | |||||
| ISSN (online) | 1096-0333 | |||||
| ISSN | 0041-008X | |||||
| DOI | 10.1016/j.taap.2015.02.004 | |||||
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| Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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