LIVIVO - Search results -

Search results

Result 1 - 10 of total 291

Search options

Article ; Online: Repair Foci as Liquid Phase Separation: Evidence and Limitations.

Miné-Hattab, Judith / Liu, Siyu / Taddei, Angela

2022 Volume 13, Issue 10

Abstract: In response to DNA double strand breaks (DSB), repair proteins accumulate at damaged sites, forming membrane-less condensates or "foci". The formation of these foci and their disassembly within the proper time window are essential for genome integrity. ... ...

Abstract	In response to DNA double strand breaks (DSB), repair proteins accumulate at damaged sites, forming membrane-less condensates or "foci". The formation of these foci and their disassembly within the proper time window are essential for genome integrity. However, how these membrane-less sub-compartments are formed, maintained and disassembled remains unclear. Recently, several studies across different model organisms proposed that DNA repair foci form via liquid phase separation. In this review, we discuss the current research investigating the physical nature of repair foci. First, we present the different models of condensates proposed in the literature, highlighting the criteria to differentiate them. Second, we discuss evidence of liquid phase separation at DNA repair sites and the limitations of this model to fully describe structures formed in response to DNA damage. Finally, we discuss the origin and possible function of liquid phase separation for DNA repair processes.
MeSH term(s)	DNA-Binding Proteins/genetics ; DNA Breaks, Double-Stranded ; DNA Repair/genetics ; DNA Damage ; DNA
Chemical Substances	DNA-Binding Proteins ; DNA (9007-49-2)
Language	English
Publishing date	2022-10-13
Publishing country	Switzerland
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	2527218-4
ISSN	2073-4425 ; 2073-4425
ISSN (online)	2073-4425
ISSN	2073-4425
DOI	10.3390/genes13101846
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Subtelomeres as Specialized Chromatin Domains.

Hocher, Antoine / Taddei, Angela

BioEssays : news and reviews in molecular, cellular and developmental biology

2020 Volume 42, Issue 5, Page(s) e1900205

Abstract: Specificities associated with chromosomal linearity are not restricted to telomeres. Here, recent results obtained on fission and budding yeast are summarized and an attempt is made to define subtelomeres using chromatin features extending beyond the ... ...

Abstract	Specificities associated with chromosomal linearity are not restricted to telomeres. Here, recent results obtained on fission and budding yeast are summarized and an attempt is made to define subtelomeres using chromatin features extending beyond the heterochromatin emanating from telomeres. Subtelomeres, the chromosome domains adjacent to telomeres, differ from the rest of the genome by their gene content, rapid evolution, and chromatin features that together contribute to organism adaptation. However, current definitions of subtelomeres are generally based on synteny and are largely gene-centered. Taking into consideration both the peculiar gene content and dynamics as well as the chromatin properties of those domains, it is discussed how chromatin features can contribute to subtelomeric properties and functions, and play a pivotal role in the emergence of subtelomeres.
MeSH term(s)	Chromatin/genetics ; Heterochromatin/genetics ; Telomere/genetics
Chemical Substances	Chromatin ; Heterochromatin
Language	English
Publishing date	2020-03-17
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	50140-2
ISSN	1521-1878 ; 0265-9247
ISSN (online)	1521-1878
ISSN	0265-9247
DOI	10.1002/bies.201900205
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 2024: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: An Inducible System for Silencing Establishment Reveals a Stepwise Mechanism in Which Anchoring at the Nuclear Periphery Precedes Heterochromatin Formation.

Loïodice, Isabelle / Garnier, Mickael / Nikolov, Ivaylo / Taddei, Angela

Cells

2021 Volume 10, Issue 11

Abstract: In eukaryotic cells, silent chromatin is mainly found at the nuclear periphery forming subnuclear compartments that favor silencing establishment. Here, we set up an inducible system to monitor silencing establishment at an ectopic locus in relation with ...

Abstract	In eukaryotic cells, silent chromatin is mainly found at the nuclear periphery forming subnuclear compartments that favor silencing establishment. Here, we set up an inducible system to monitor silencing establishment at an ectopic locus in relation with its subnuclear localization in budding yeast. We previously showed that introducing LacI bound
MeSH term(s)	Cell Cycle/genetics ; Gene Silencing ; Genetic Loci ; Heterochromatin/metabolism ; Nucleosomes/metabolism ; Protein Binding ; Protein Stability ; Saccharomyces cerevisiae/cytology ; Saccharomyces cerevisiae/metabolism ; Saccharomyces cerevisiae Proteins/metabolism
Chemical Substances	Heterochromatin ; Nucleosomes ; Saccharomyces cerevisiae Proteins
Language	English
Publishing date	2021-10-20
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2661518-6
ISSN	2073-4409 ; 2073-4409
ISSN (online)	2073-4409
ISSN	2073-4409
DOI	10.3390/cells10112810
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Physical principles and functional consequences of nuclear compartmentalization in budding yeast.

Miné-Hattab, Judith / Taddei, Angela

Current opinion in cell biology

2019 Volume 58, Page(s) 105–113

Abstract: One striking feature of eukaryotic nuclei is the existence of discrete regions, in which specific factors concentrate while others are excluded, thus forming microenvironments with different molecular compositions and biological functions. These domains ... ...

Abstract	One striking feature of eukaryotic nuclei is the existence of discrete regions, in which specific factors concentrate while others are excluded, thus forming microenvironments with different molecular compositions and biological functions. These domains are often referred to as subcompartments even though they are not membrane enclosed. Despite their functional importance the physical nature of these structures remains largely unknown. Here, we describe how the Saccharomyces cerevisiae nucleus is compartmentalized and discuss possible physical models underlying the formation and maintenance of chromatin associated subcompartments. Focusing on three particular examples, the nucleolus, silencing foci, and repair foci, we discuss the biological implications of these different models as well as possible approaches to challenge them in living cells.
MeSH term(s)	Cell Nucleolus/chemistry ; Cell Nucleus/chemistry ; Cell Nucleus/physiology ; Chromatin/chemistry ; Chromosomes/chemistry ; DNA Repair ; Gene Silencing ; Saccharomyces cerevisiae/cytology ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae Proteins/genetics
Chemical Substances	Chromatin ; Saccharomyces cerevisiae Proteins
Language	English
Publishing date	2019-03-29
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1026381-0
ISSN	1879-0410 ; 0955-0674
ISSN (online)	1879-0410
ISSN	0955-0674
DOI	10.1016/j.ceb.2019.02.005
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2963: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Size‐dependent sensitivity of stream amphipods indicates population‐level responses to chemical pollution

Taddei, Anja / Räsänen, Katja / Burdon, Francis J

Freshwater biology. 2021 Apr., v. 66, no. 4

2021

Abstract: Global change assessments have typically ignored synthetic chemical pollution, despite the rapid increase of pharmaceuticals, pesticides, and industrial chemicals in the environment. The paucity of research on the ecological effects of these ... ...

Abstract	Global change assessments have typically ignored synthetic chemical pollution, despite the rapid increase of pharmaceuticals, pesticides, and industrial chemicals in the environment. The paucity of research on the ecological effects of these micropollutants undermines our efforts to address the freshwater biodiversity crisis. Understanding the responses of individual organisms to chemical pollution should help address this knowledge gap because individual‐level effects can cascade across populations, communities, and ecosystems with devastating consequences. Inputs of treated municipal wastewater are a major source of micropollutants in receiving environments. Here, we assessed population and individual‐level influences of treated wastewater on freshwater gammarid amphipods (Gammarus spp.) in Swiss lowland streams in situ and tested effects of a micropollutant mixture on individual Gammarus fossarum using a common garden laboratory experiment. We hypothesised that population‐level effects of wastewater are mediated through asymmetric sensitivities of juvenile and adult gammarids to chemical pollutants. We expected that life‐stage specific sensitivities would reflect allometric theory relating body size to a wide range of organismal characteristics, including metabolism, growth, and mortality. At the population level (i.e. field survey), we observed greatly reduced abundances of juvenile gammarids downstream of the wastewater discharge in three of the six sites surveyed, indicating the potential for demographic effects of pollution. At the individual level in a field transplant experiment, we found that the presence of wastewater led to a steeper positive relationship between gammarid body size and leaf consumption. In the laboratory experiment, we found that micropollutants had negative effects on consumption and growth rates, but a positive effect on survival. Differences in the relationship between gammarid body size and performance were subtle across treatments, although flatter slopes in growth appear to be a consistent response to chemical pollution. Faster growth rates appeared to be connected with reduced survival, placing individuals in control treatments and/or with smaller body size at greatest risk of mortality. Notably, juvenile gammarids had faster growth rates and lower survival than adults when exposed to micropollutants. Our results demonstrate the potential for negative impacts of micropollutants in freshwater ecosystems. However, the results also show that organismal responses to chemical pollutants can be complex, whereby impacts at the individual level may act counter‐intuitively to population‐level dynamics. This highlights the need for more realistic experiments to better assess how organismal responses depend on life stage and body size, and how individual‐level effects propagate to higher levels of biological organisation. Our study shows how allometric theory can be used to examine the effects of stressors on underlying organismal biology, population demographics, and link with broader macroecological patterns.
Keywords	Gammarus fossarum ; adults ; allometry ; biodiversity ; body size ; demographic statistics ; drugs ; freshwater ; global change ; juveniles ; laboratory experimentation ; leaves ; limnology ; metabolism ; mortality ; municipal wastewater ; pollution ; risk ; streams ; surveys
Language	English
Dates of publication	2021-04
Size	p. 765-784.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	NAL-AP-2-clean ; JOURNAL ARTICLE
ZDB-ID	121180-8
ISSN	0046-5070
ISSN	0046-5070
DOI	10.1111/fwb.13677
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 77/704: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Virtual Place Identity

Fermani, A. / Giaconi, C. / Taddei, A. / Del Moral Arroyo, G.

la percezione dell’autoefficacia negli educatori in formazione durante l’emergenza Covid-19

2020

Keywords	covid19
Language	Italian
Publisher	EUM
Publishing country	it
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Preliminary Analysis of the Presence of Bacterial Azurin Coding Gene in CRC Patients and Correlation with the Microbiota Composition.

Iozzo, Marta / Vitali, Francesco / Chiellini, Carolina / Gammuto, Leandro / Taddei, Antonio / Amedei, Amedeo / Fani, Renato

Frontiers in bioscience (Landmark edition)

2022 Volume 27, Issue 11, Page(s) 305

Abstract: Background: Azurin, a bacterial cupredoxin firstly isolated from the bacterium : Aims: In this work we have explored the relationship possibly existing between azurin and colorectal cancer (CRC), in light of the evidence that microbial imbalance can ... ...

Abstract	Background: Azurin, a bacterial cupredoxin firstly isolated from the bacterium Aims: In this work we have explored the relationship possibly existing between azurin and colorectal cancer (CRC), in light of the evidence that microbial imbalance can lead to CRC progression. Methodology/results: To this aim, the presence of azurin coding gene in the DNA extracted from saliva, stool, and biopsy samples of 10 CRC patients and 10 healthy controls was evaluated by real-time PCR using primers specifically designed to target the azurin coding gene from different bacterial groups. The correlation of the previously obtained microbiota data with real-time PCR results evidenced a "preferential" enrichment of seven bacterial groups in some samples than in others, even though no statistical significance was detected between controls and CRC. The subset of azurin gene-harbouring bacterial groups was representative of the entire community. Conclusions: Despite the lack of statistical significance between healthy and diseased patients, HTS data analysis highlighted a kind of "preferential" enrichment of seven bacterial groups harbouring the azurin gene in some samples than in others.
Language	English
Publishing date	2022-11-23
Publishing country	Singapore
Document type	Journal Article
ZDB-ID	2704569-9
ISSN	2768-6698 ; 2768-6698
ISSN (online)	2768-6698
ISSN	2768-6698
DOI	10.31083/j.fbl2711305
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Social contact, own-group recognition bias and visual attention to faces.

Brunet, Malvina / Taddei, Anaïs / Py, Jacques / Paubel, Pierre-Vincent / Tredoux, Colin

British journal of psychology (London, England : 1953)

2022 Volume 114 Suppl 1, Page(s) 112–133

Abstract: The own-group recognition bias (OGRB) has been consistently linked to social contact in theoretical accounts. Indeed, social contact is assumed by most authors to underlie the perceptual expertise of out-groups' faces. However, little is known ... ...

Abstract	The own-group recognition bias (OGRB) has been consistently linked to social contact in theoretical accounts. Indeed, social contact is assumed by most authors to underlie the perceptual expertise of out-groups' faces. However, little is known empirically about how it might impact face-processing strategies. We tested the proposition that social interaction would improve the face recognition performance of another group by modulating visual strategies for different face areas. In Experiment 1, we studied visual processes using an eye tracker during a person's first live encounter with a particular member of their own group (European) or an outgroup (African) to explore how increasing familiarity during a first interaction influences face-processing strategies. In Experiments 2 and 3, we explored the effect of simulated intergroup contact on face recognition accuracy, while simultaneously studying the impact of contact on visual attention strategies that occur during recognition (Experiment 2) and encoding (Experiment 3). The results showed a strong OGRB and a difference in visual processes based on the ethnic group of the targets. Although a single interaction is not sufficient to reduce the OGRB, familiarization during a live interaction (Experiment 1) and virtual social contact (Experiment 2) had an impact on the visual strategies employed.
MeSH term(s)	Humans ; Recognition, Psychology ; Bias ; Facial Recognition ; Pattern Recognition, Visual
Language	English
Publishing date	2022-10-27
Publishing country	England
Document type	Journal Article
ZDB-ID	220659-6
ISSN	2044-8295
ISSN (online)	2044-8295
DOI	10.1111/bjop.12603
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Ui I Zs.237: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: In vivo tracking of functionally tagged Rad51 unveils a robust strategy of homology search.

Liu, Siyu / Miné-Hattab, Judith / Villemeur, Marie / Guerois, Raphaël / Pinholt, Henrik Dahl / Mirny, Leonid A / Taddei, Angela

Nature structural & molecular biology

2023 Volume 30, Issue 10, Page(s) 1582–1591

Abstract: Homologous recombination (HR) is a major pathway to repair DNA double-strand breaks (DSB). HR uses an undamaged homologous DNA sequence as a template for copying the missing information, which requires identifying a homologous sequence among megabases of ...

Abstract	Homologous recombination (HR) is a major pathway to repair DNA double-strand breaks (DSB). HR uses an undamaged homologous DNA sequence as a template for copying the missing information, which requires identifying a homologous sequence among megabases of DNA within the crowded nucleus. In eukaryotes, the conserved Rad51-single-stranded DNA nucleoprotein filament (NPF) performs this homology search. Although NPFs have been extensively studied in vitro by molecular and genetic approaches, their in vivo formation and dynamics could not thus far be assessed due to the lack of functional tagged versions of Rad51. Here we develop and characterize in budding yeast the first fully functional, tagged version of Rad51. Following induction of a unique DSB, we observe Rad51-ssDNA forming exceedingly long filaments, spanning the whole nucleus and eventually contacting the donor sequence. Emerging filaments adopt a variety of shapes not seen in vitro and are modulated by Rad54 and Srs2, shedding new light on the function of these factors. The filaments are also dynamic, undergoing rounds of compaction and extension. Our biophysical models demonstrate that formation of extended filaments, and particularly their compaction-extension dynamics, constitute a robust search strategy, allowing DSB to rapidly explore the nuclear volume and thus enable efficient HR.
MeSH term(s)	Saccharomyces cerevisiae Proteins/genetics ; Saccharomyces cerevisiae Proteins/metabolism ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; DNA Helicases/metabolism ; Rad51 Recombinase/genetics ; Rad51 Recombinase/metabolism ; DNA/metabolism ; DNA, Single-Stranded/genetics ; DNA, Single-Stranded/metabolism
Chemical Substances	Saccharomyces cerevisiae Proteins ; DNA-Binding Proteins ; DNA Helicases (EC 3.6.4.-) ; Rad51 Recombinase (EC 2.7.7.-) ; DNA (9007-49-2) ; DNA, Single-Stranded
Language	English
Publishing date	2023-08-21
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2126708-X
ISSN	1545-9985 ; 1545-9993
ISSN (online)	1545-9985
ISSN	1545-9993
DOI	10.1038/s41594-023-01065-w
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 4101: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 56: Show issues

Order via subito

Details ▾

Article ; Online: Evaluation of identification methods for cryptic Bactrocera dorsalis (Diptera: Tephritidae) specimens: combining morphological and molecular techniques.

Charbonnel, Emeline / Chapuis, Marie-Pierre / Taddei, Andrea / Schutze, Mark K / Starkie, Melissa L / Benoit, Laure / Mouttet, Raphaëlle / Ouvrard, David

Journal of economic entomology

2023 Volume 116, Issue 6, Page(s) 2193–2200

Abstract: The potential for population genomics to elucidate invasion pathways of a species is limited by taxonomic identification issues. The Oriental fruit fly pest, Bactrocera dorsalis (Hendel) belongs to a complex in which several sympatric species are ... ...

Abstract	The potential for population genomics to elucidate invasion pathways of a species is limited by taxonomic identification issues. The Oriental fruit fly pest, Bactrocera dorsalis (Hendel) belongs to a complex in which several sympatric species are attracted to the same lure used in trapping and are morphologically cryptic and/or reported to hybridize. In this study, we evaluated the taxonomic ambiguity between B. dorsalis and 2 major cryptic species, based on morphological expertise and 289 target specimens sampled across the whole distribution range. Specimens were then subjected to DNA sequence analyses of the COI mitochondrial barcode and the EIF3L nuclear marker to evaluate the potential for molecular identification, in particular for specimens for which morphological identification was inconclusive. To this aim, we produced reference datasets with DNA sequences from target specimens whose morphological identification was unambiguous, which we complemented with 56 new DNA sequences from closest relatives and 76 published and curated DNA sequences of different species in the complex. After the necessary morphological observation, about 3.5% of the target dataset and 47.6% of the specimens from Southeast Asian islands displayed ambiguous character states shared with B. carambolae and/or B. occipitalis. Critical interpretation of DNA sequence data solved morphological ambiguities only when combining both mitochondrial and nuclear markers. COI discriminated B. dorsalis from 5 species; EIF3L and ITS from another species. We recommend this procedure to ensure correct identification of B. dorsalis specimens in population genetics studies and surveillance programs.
MeSH term(s)	Animals ; Tephritidae/genetics ; Sequence Analysis, DNA ; Mitochondria
Language	English
Publishing date	2023-10-12
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3031-4
ISSN	1938-291X ; 0022-0493
ISSN (online)	1938-291X
ISSN	0022-0493
DOI	10.1093/jee/toad178
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 1118: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito