LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 6 of total 6

Search options

  1. Article ; Online: Oral Administration of PLGA Nanoparticles to Deliver Antisense Oligonucleotides to Inflammatory Lesions in the Gastrointestinal Tract.

    Yagi, Yuta / Liu, Yiwei / Li, Jinting / Shimada, Shunsuke / Ohkouchi, Munetaka / Taguchi, Yasushi / Nii, Teruki / Mori, Takeshi / Katayama, Yoshiki

    Biological & pharmaceutical bulletin

    2024  Volume 47, Issue 4, Page(s) 848–855

    Abstract: In this study, we prepared antisense oligonucleotide (ASO)-encapsulated nanoparticles (NPs) with a suitable profile for oral administration for the treatment of inflammatory bowel disease (IBD). We chose a water-in-oil-in-water (w/o/w) method to prepare ... ...

    Abstract In this study, we prepared antisense oligonucleotide (ASO)-encapsulated nanoparticles (NPs) with a suitable profile for oral administration for the treatment of inflammatory bowel disease (IBD). We chose a water-in-oil-in-water (w/o/w) method to prepare the NPs using poly(lactide-co-glycolide) as a matrix and Pluronic as a stabilizer. The obtained NPs had a suitable diameter (158 nm) for the penetration of the mucus layer, endocytic uptake by enterocytes, and accumulation in inflammatory lesions in the intestine. The amount of ASOs in the NPs was relatively large (6.41% (w/w)). When the NPs were stably dispersed in solutions that mimicked gastrointestinal (GI) juice, minimal leakage of ASOs was demonstrated over the required period. The NPs were administered orally to mice with colitis induced by dextran sodium sulfate, which reduced target gene expression in the colons and rectums of the mice, whereas naked ASO administration caused no reduction in gene expression. Thus, the NPs have the potential of promising oral carriers of ASOs for the treatment of IBD that specifically target inflammatory lesions in the GI tract, thereby reducing the non-specific toxic effects of ASOs.
    MeSH term(s) Animals ; Mice ; Oligonucleotides, Antisense ; Inflammatory Bowel Diseases/drug therapy ; Administration, Oral ; Nanoparticles ; Water
    Chemical Substances Oligonucleotides, Antisense ; Water (059QF0KO0R)
    Language English
    Publishing date 2024-03-22
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 1150271-x
    ISSN 1347-5215 ; 0918-6158
    ISSN (online) 1347-5215
    ISSN 0918-6158
    DOI 10.1248/bpb.b23-00769
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 1474: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: Determination of Region-Specific Roles of the M

    Igarashi-Hisayoshi, Yoko / Ihara, Eikichi / Bai, Xiaopeng / Higashi, Chika / Ikeda, Hiroko / Tanaka, Yoshimasa / Hirano, Mayumi / Ogino, Haruei / Chinen, Takatoshi / Taguchi, Yasushi / Ogawa, Yoshihiro

    Digestive diseases and sciences

    2022  Volume 68, Issue 2, Page(s) 439–450

    Abstract: Background: The specific role of the M: Aims: The objective of this study was to determine the region-specific role of the M: Methods: We developed a novel positive allosteric modulator (PAM) for the M: Results: PAM-369 selectively potentiated ... ...

    Abstract Background: The specific role of the M
    Aims: The objective of this study was to determine the region-specific role of the M
    Methods: We developed a novel positive allosteric modulator (PAM) for the M
    Results: PAM-369 selectively potentiated the M
    Conclusions: This study provided the first direct evidence that the M
    MeSH term(s) Animals ; Humans ; Mice ; Rats ; Carbachol/pharmacology ; Gastrointestinal Motility/genetics ; Gastrointestinal Motility/physiology ; Muscle Contraction ; Receptor, Muscarinic M2/genetics ; Receptor, Muscarinic M2/metabolism ; Receptor, Muscarinic M3/genetics ; Receptor, Muscarinic M3/metabolism ; Receptors, Muscarinic/physiology ; Swine
    Chemical Substances Carbachol (8Y164V895Y) ; Receptor, Muscarinic M2 ; Receptor, Muscarinic M3 ; Receptors, Muscarinic
    Language English
    Publishing date 2022-08-10
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 304250-9
    ISSN 1573-2568 ; 0163-2116
    ISSN (online) 1573-2568
    ISSN 0163-2116
    DOI 10.1007/s10620-022-07637-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Ui V Zs.35: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: Stability of DNA/Td3717 complexes for gene transfection, defined by the size and polydispersity of the complex.

    Kuriyama, Shinichi / Taguchi, Yasushi / Watanabe, Kazuto / Nishimura, Kanako / Yanagibashi, Kazutoshi / Katayama, Yoshiki / Niidome, Takuro

    Bioorganic & medicinal chemistry

    2009  Volume 17, Issue 22, Page(s) 7643–7646

    Abstract: The amphiphilic alpha-helical peptide, Td3717, is a bi-functional synthetic peptide that acts as both a polycation for DNA binding and a ligand for targeted delivery to tumor cells. Td3717 forms a stable complex with plasmid DNA, and the complex ... ...

    Abstract The amphiphilic alpha-helical peptide, Td3717, is a bi-functional synthetic peptide that acts as both a polycation for DNA binding and a ligand for targeted delivery to tumor cells. Td3717 forms a stable complex with plasmid DNA, and the complex maintained high transfection efficiency after storage at 4 degrees C for six months and after four freeze/thaw cycles. During the storage and freeze/thaw cycling, the particle size of the DNA/Td3717 complex remained less than 100nm. The size of the complex is an important factor for its internalization into cells via the endocytosis pathway; therefore, the stability of the particles will strongly contribute to high transfection efficiencies after storage and repeated freezing/thawing.
    MeSH term(s) Amino Acid Sequence ; Animals ; Carrier Proteins/chemical synthesis ; Carrier Proteins/chemistry ; Carrier Proteins/genetics ; Carrier Proteins/metabolism ; Cells, Cultured ; Cercopithecus aethiops ; DNA/chemistry ; DNA/genetics ; DNA/metabolism ; Drug Carriers/chemistry ; Drug Carriers/metabolism ; Endocytosis ; Freezing ; Haplorhini ; Molecular Sequence Data ; Protein Structure, Secondary ; Specimen Handling ; Transfection/methods ; Vero Cells
    Chemical Substances Carrier Proteins ; Drug Carriers ; Td3717 protein ; DNA (9007-49-2)
    Language English
    Publishing date 2009-11-15
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1161284-8
    ISSN 1464-3391 ; 0968-0896
    ISSN (online) 1464-3391
    ISSN 0968-0896
    DOI 10.1016/j.bmc.2009.09.049
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 3815: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article: Improvement of peptide vectors for gene delivery with active targeting profiles for phosphatidylserine.

    Kuriyama, Shinichi / Taguchi, Yasushi / Nishimura, Kanako / Yanagibashi, Kazutoshi / Katayama, Yoshiki / Niidoime, Takuro

    Journal of peptide science : an official publication of the European Peptide Society

    2009  Volume 15, Issue 2, Page(s) 114–119

    Abstract: A cationic peptide, Td3701, which was derived from factor VIII that has affinity with phosphatidylserine (PS), showed efficient transfection ability for cells that express PS on the cell surface. PS is exposed on tumor cell surfaces therefore we have ... ...

    Abstract A cationic peptide, Td3701, which was derived from factor VIII that has affinity with phosphatidylserine (PS), showed efficient transfection ability for cells that express PS on the cell surface. PS is exposed on tumor cell surfaces therefore we have focused on PS as the target molecule for tumor specific gene delivery. In this article, to improve transfection efficiency and specificity in targeting tumor cells, some amino acid residues of Td3701 were replaced. The resulting peptide, Td3717, shows higher transfection efficiency (more than 30 times that of Td3701). The transfection efficiency was dependent on the amount of PS on the cell surface, suggesting that Td3717 bound with plasmid DNA could recognize PS on the cell surface. Td3717 is expected to be useful as an efficient gene carrier molecule specific to PS-presenting tumor cells.
    MeSH term(s) Amino Acid Sequence ; Animals ; Cell Line, Tumor ; Cercopithecus aethiops ; Gene Transfer Techniques ; Genetic Vectors/genetics ; Genetic Vectors/metabolism ; Humans ; Molecular Sequence Data ; Peptides/chemical synthesis ; Peptides/chemistry ; Peptides/genetics ; Peptides/metabolism ; Phosphatidylserines/metabolism ; Vero Cells
    Chemical Substances Peptides ; Phosphatidylserines
    Language English
    Publishing date 2009-02
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1234416-3
    ISSN 1099-1387 ; 1075-2617
    ISSN (online) 1099-1387
    ISSN 1075-2617
    DOI 10.1002/psc.1104
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6200: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article: An analysis of the chest wall motions using the dynamic MRI in healthy elder subjects.

    Kondo, Tetsuri / Kobayashi, Ichiro / Taguchi, Yasushi / Hayama, Naoki / Tajiri, Sakurako / Yanagimachi, Noriharu

    The Tokai journal of experimental and clinical medicine

    2005  Volume 30, Issue 1, Page(s) 15–20

    Abstract: The objective of this study was to compare respiratory motions of the chest wall in the healthy elder subjects (N = 5; mean age, 71 years old) with those in healthy young subjects (n = 9; mean age, 29 years old). Thirty sequential images (scanning time, ... ...

    Abstract The objective of this study was to compare respiratory motions of the chest wall in the healthy elder subjects (N = 5; mean age, 71 years old) with those in healthy young subjects (n = 9; mean age, 29 years old). Thirty sequential images (scanning time, 0.4s per image) of dynamic MRI on sagittal and coronal planes were obtained while the subjects were deeply breathing. Lung volume change was simultaneously measured with pneumotachometer. In the elder subjects, dimensions of the middle and posterior parts of the diaphragm were linearly related to instantaneous lung volume. There were poor correlation between motion of the anterior diaphragm and transverse motions of the upper rib-cage and lower rib-cage. The contribution of individual part of the chest wall motion to a unit lung volume change, assessed by slope of the linear regression line, in elder subjects were not significantly different from those in young subjects. Either in the elder or young subjects, the middle and posterior parts of the diaphragm moved coordinately. We conclude that chest wall motion in the healthy elder subjects is not different from that in healthy young people and that middle and posterior parts of the diaphragm act as one functional unit during deep breathing.
    MeSH term(s) Adult ; Aged ; Aging/physiology ; Diaphragm/physiology ; Health ; Humans ; Lung/physiology ; Magnetic Resonance Imaging ; Male ; Movement/physiology ; Respiration ; Thoracic Wall/physiology
    Language English
    Publishing date 2005-04
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 431622-8
    ISSN 2185-2243 ; 0385-0005
    ISSN (online) 2185-2243
    ISSN 0385-0005
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.B 2001: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article: Peptide vector for gene delivery with high affinity for phosphatidylserine.

    Kuriyama, Shinichi / Taguchi, Yasushi / Nishimura, Kanako / Mizuguchi, Kiyoshi / Kobayashi, Kazutoshi / Katayama, Yoshiki / Yanagibashi, Kazutoshi / Niidome, Takuro

    Journal of peptide science : an official publication of the European Peptide Society

    2006  Volume 12, Issue 10, Page(s) 626–632

    Abstract: Since phosphatidylserine (PS) is known to translocate to the external face of the plasma membrane when the cell membrane becomes disordered, we decided to focus our attention on PS as a target molecule for gene delivery. In this paper, the novel peptide ... ...

    Abstract Since phosphatidylserine (PS) is known to translocate to the external face of the plasma membrane when the cell membrane becomes disordered, we decided to focus our attention on PS as a target molecule for gene delivery. In this paper, the novel peptide Td3701 was designed, synthesized, and characterized for its physico-chemico-biological properties. Td3701 simultaneously exhibited both characters as a DNA carrier and a sensor probe for active targeting, which seemed to be triggered by structural changes in the presence of PS. This is a very unique character among nonviral vectors, and it is believed that Td3701 could be used for selective gene delivery.
    MeSH term(s) Amino Acid Sequence ; Animals ; Cercopithecus aethiops ; Circular Dichroism ; DNA/chemistry ; DNA/metabolism ; Factor VIII/chemistry ; Gene Transfer Techniques ; Genetic Vectors/metabolism ; Liposomes ; Molecular Sequence Data ; Peptides/administration & dosage ; Peptides/chemistry ; Peptides/metabolism ; Phosphatidylserines/metabolism ; Plasmids/chemistry ; Plasmids/genetics ; Protein Structure, Tertiary ; Transfection ; Vero Cells
    Chemical Substances Liposomes ; Peptides ; Phosphatidylserines ; Factor VIII (9001-27-8) ; DNA (9007-49-2)
    Language English
    Publishing date 2006-10
    Publishing country England
    Document type Journal Article
    ZDB-ID 1234416-3
    ISSN 1099-1387 ; 1075-2617
    ISSN (online) 1099-1387
    ISSN 1075-2617
    DOI 10.1002/psc.768
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6200: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top