LIVIVO - Search results -

Search results

Result 1 - 10 of total 39

Search options

Article ; Online: Taking cues from convalescence to improve vaccines against hepatitis C virus.

Sreekumar, Bharath K / Taha, Taha Y / Ott, Melanie

2022 Volume 132, Issue 15

Abstract: Hepatitis C virus (HCV) infection remains a worldwide public health issue despite direct-acting antivirals. A substantial proportion of infected individuals (15%-45%) spontaneously clear repeated HCV infections with genetically different viruses by ... ...

Abstract	Hepatitis C virus (HCV) infection remains a worldwide public health issue despite direct-acting antivirals. A substantial proportion of infected individuals (15%-45%) spontaneously clear repeated HCV infections with genetically different viruses by generating broadly neutralizing antibodies (bNAbs). However, translating this response into an effective vaccine strategy has been unsuccessful. In this issue of the JCI, Frumento and colleagues report on their study of bNAb evolution longitudinally in convalescent individuals with repeated infections. Using pseudotyped viruses, well-characterized monoclonal antibodies, and complex modeling, the authors show that multiple exposures to antigenically related, antibody-sensitive viral envelope proteins induced potent bNAbs. This work provides valuable insight into the best strategies for developing HCV vaccines in the future that may successfully reproduce the immunity induced during natural exposures.
MeSH term(s)	Antibodies, Neutralizing ; Antiviral Agents ; Broadly Neutralizing Antibodies ; Convalescence ; Cues ; Hepacivirus ; Hepatitis C ; Hepatitis C Antibodies ; Hepatitis C, Chronic ; Humans ; Vaccines/metabolism ; Viral Envelope Proteins ; Viral Hepatitis Vaccines
Chemical Substances	Antibodies, Neutralizing ; Antiviral Agents ; Broadly Neutralizing Antibodies ; Hepatitis C Antibodies ; Vaccines ; Viral Envelope Proteins ; Viral Hepatitis Vaccines
Language	English
Publishing date	2022-08-01
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Comment
ZDB-ID	3067-3
ISSN	1558-8238 ; 0021-9738
ISSN (online)	1558-8238
ISSN	0021-9738
DOI	10.1172/JCI161819
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ua VI Zs.184: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- See ZB MED holdings
- Order with fees

Article: Design and Optimization of a

Taha, Taha Y / Townsend, Michael B / Pohl, Jan / Karem, Kevin L / Damon, Inger K / Mbala Kingebeni, Placide / Muyembe Tamfum, Jean-Jacques / Martin, James W / Pittman, Phillip R / Huggins, John W / Satheshkumar, Panayampalli S / Bagarozzi, Dennis A / Reynolds, Mary G / Hughes, Laura J

Pathogens (Basel, Switzerland)

2023 Volume 12, Issue 3

Abstract: Monkeypox virus (MPXV), a member of ... ...

Abstract	Monkeypox virus (MPXV), a member of the
Language	English
Publishing date	2023-03-01
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2695572-6
ISSN	2076-0817
ISSN	2076-0817
DOI	10.3390/pathogens12030396
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: A single inactivating amino acid change in the SARS-CoV-2 NSP3 Mac1 domain attenuates viral replication and pathogenesis

Taha, Taha Y / Suryawanshi, Rahul K / Chen, Irene P / Correy, Galen J / O'Leary, Patrick C / Jogalekar, Manasi P / McCavitt-Malvido, Maria / Diolaiti, Morgan E / Kimmerly, Gabriella R / Tsou, Chia-Lin / Martinez-Sobrido, Luis / Krogan, Nevan J / Ashworth, Alan / Fraser, James S / Ott, Melanie

bioRxiv : the preprint server for biology

2023

Abstract: Despite unprecedented efforts, our therapeutic arsenal against SARS-CoV-2 remains limited. The conserved macrodomain 1 (Mac1) in NSP3 is an enzyme exhibiting ADP-ribosylhydrolase activity and a possible drug target. To determine the therapeutic potential ...

Abstract	Despite unprecedented efforts, our therapeutic arsenal against SARS-CoV-2 remains limited. The conserved macrodomain 1 (Mac1) in NSP3 is an enzyme exhibiting ADP-ribosylhydrolase activity and a possible drug target. To determine the therapeutic potential of Mac1 inhibition, we generated recombinant viruses and replicons encoding a catalytically inactive NSP3 Mac1 domain by mutating a critical asparagine in the active site. While substitution to alanine (N40A) reduced catalytic activity by ~10-fold, mutations to aspartic acid (N40D) reduced activity by ~100-fold relative to wildtype. Importantly, the N40A mutation rendered Mac1 unstable
Language	English
Publishing date	2023-05-10
Publishing country	United States
Document type	Preprint
DOI	10.1101/2023.04.18.537104
Database	MEDical Literature Analysis and Retrieval System OnLINE

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article ; Online: Modulation of hepatitis B virus pregenomic RNA stability and splicing by histone deacetylase 5 enhances viral biosynthesis.

Taha, Taha Y / Anirudhan, Varada / Limothai, Umaporn / Loeb, Daniel D / Petukhov, Pavel A / McLachlan, Alan

PLoS pathogens

2020 Volume 16, Issue 8, Page(s) e1008802

Abstract: Hepatitis B virus (HBV) is a worldwide health problem without curative treatments. Investigation of the regulation of HBV biosynthesis by class I and II histone deacetylases (HDACs) demonstrated that catalytically active HDAC5 upregulates HBV ... ...

Abstract	Hepatitis B virus (HBV) is a worldwide health problem without curative treatments. Investigation of the regulation of HBV biosynthesis by class I and II histone deacetylases (HDACs) demonstrated that catalytically active HDAC5 upregulates HBV biosynthesis. HDAC5 expression increased both the stability and splicing of the HBV 3.5 kb RNA without altering the translational efficiency of the viral pregenomic or spliced 2.2 kb RNAs. Together, these observations point to a broader role of HDAC5 in regulating RNA splicing and transcript stability while specifically identifying a potentially novel approach toward antiviral HBV therapeutic development.
MeSH term(s)	Gene Expression Regulation, Viral ; Genome, Viral ; Hep G2 Cells ; Hepatitis B/virology ; Hepatitis B virus/genetics ; Hepatitis B virus/metabolism ; Histone Deacetylases/genetics ; Histone Deacetylases/metabolism ; Humans ; RNA Stability ; RNA, Viral/biosynthesis ; RNA, Viral/chemistry ; Transcription, Genetic ; Virus Replication
Chemical Substances	RNA, Viral ; Histone Deacetylases (EC 3.5.1.98)
Language	English
Publishing date	2020-08-21
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2205412-1
ISSN	1553-7374 ; 1553-7366
ISSN (online)	1553-7374
ISSN	1553-7366
DOI	10.1371/journal.ppat.1008802
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Histone deacetylase inhibitor-based chromatin precipitation for identification of targeted genomic loci.

Hanigan, Thomas W / Danes, Jeanne M / Taha, Taha Y / Frasor, Jonna / Petukhov, Pavel A

Journal of biological methods

2017 Volume 5, Issue 1

Abstract: Histone deacetylase (HDAC) catalyzes the removal of acetyl marks from histones, effectively regulating gene expression. Genome wide chromatin immunoprecipitation (ChIP) studies have shown HDACs are present on numerous active and repressed genes. However, ...

Abstract	Histone deacetylase (HDAC) catalyzes the removal of acetyl marks from histones, effectively regulating gene expression. Genome wide chromatin immunoprecipitation (ChIP) studies have shown HDACs are present on numerous active and repressed genes. However, HDAC inhibitors (HDACi) only regulate a small subset of this population in a cell type dependent fashion. To determine genomic locations directly targeted by HDACi, we developed a chromatin precipitation method using a photoreactive HDAC inhibitor probe (photomate). We validate this method by analyzing several canonical HDACi regulated genes, CDKN1A and FOSL1, and compare it to traditional ChIP using HDAC1 antibodies. We show that HDACi target HDACs bound at the promoter regions but not gene bodies, differing from HDAC1 antibody-based ChIP in the case of CDKN1A. This approach is anticipated to be useful for genome wide studies to identify the subset of genes directly regulated by an HDACi in a given cell type.
Language	English
Publishing date	2017-02-09
Publishing country	United States
Document type	Journal Article
ISSN	2326-9901
ISSN	2326-9901
DOI	10.14440/jbm.2018.216
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Rapid assembly of SARS-CoV-2 genomes reveals attenuation of the Omicron BA.1 variant through NSP6.

Taha, Taha Y / Chen, Irene P / Hayashi, Jennifer M / Tabata, Takako / Walcott, Keith / Kimmerly, Gabriella R / Syed, Abdullah M / Ciling, Alison / Suryawanshi, Rahul K / Martin, Hannah S / Bach, Bryan H / Tsou, Chia-Lin / Montano, Mauricio / Khalid, Mir M / Sreekumar, Bharath K / Renuka Kumar, G / Wyman, Stacia / Doudna, Jennifer A / Ott, Melanie

Nature communications

2023 Volume 14, Issue 1, Page(s) 2308

Abstract: Although the SARS-CoV-2 Omicron variant (BA.1) spread rapidly across the world and effectively evaded immune responses, its viral fitness in cell and animal models was reduced. The precise nature of this attenuation remains unknown as generating ... ...

Abstract	Although the SARS-CoV-2 Omicron variant (BA.1) spread rapidly across the world and effectively evaded immune responses, its viral fitness in cell and animal models was reduced. The precise nature of this attenuation remains unknown as generating replication-competent viral genomes is challenging because of the length of the viral genome (~30 kb). Here, we present a plasmid-based viral genome assembly and rescue strategy (pGLUE) that constructs complete infectious viruses or noninfectious subgenomic replicons in a single ligation reaction with >80% efficiency. Fully sequenced replicons and infectious viral stocks can be generated in 1 and 3 weeks, respectively. By testing a series of naturally occurring viruses as well as Delta-Omicron chimeric replicons, we show that Omicron nonstructural protein 6 harbors critical attenuating mutations, which dampen viral RNA replication and reduce lipid droplet consumption. Thus, pGLUE overcomes remaining barriers to broadly study SARS-CoV-2 replication and reveals deficits in nonstructural protein function underlying Omicron attenuation.
MeSH term(s)	Animals ; Coronavirus Nucleocapsid Proteins/genetics ; COVID-19 ; Genome, Viral/genetics ; RNA, Viral/genetics ; SARS-CoV-2/genetics ; Subgenomic RNA/genetics
Chemical Substances	Coronavirus Nucleocapsid Proteins ; NSP6 protein, SARS-CoV-2 ; RNA, Viral ; Subgenomic RNA
Language	English
Publishing date	2023-04-21
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-023-37787-0
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Rapid assembly of SARS-CoV-2 genomes reveals attenuation of the Omicron BA.1 variant through NSP6.

Taha, Taha Y / Chen, Irene P / Hayashi, Jennifer M / Tabata, Takako / Walcott, Keith / Kimmerly, Gabriella R / Syed, Abdullah M / Ciling, Alison / Suryawanshi, Rahul K / Martin, Hannah S / Bach, Bryan H / Tsou, Chia-Lin / Montano, Mauricio / Khalid, Mir M / Sreekumar, Bharath K / Kumar, G Renuka / Wyman, Stacia / Doudna, Jennifer A / Ott, Melanie

bioRxiv : the preprint server for biology

2023

Abstract	Although the SARS-CoV-2 Omicron variant (BA.1) spread rapidly across the world and effectively evaded immune responses, its viral fitness in cell and animal models was reduced. The precise nature of this attenuation remains unknown as generating replication-competent viral genomes is challenging because of the length of the viral genome (30kb). Here, we designed a plasmid-based viral genome assembly and resc ue strategy (pGLUE) that constructs complete infectious viruses or noninfectious subgenomic replicons in a single ligation reaction with >80% efficiency. Fully sequenced replicons and infectious viral stocks can be generated in 1 and 3 weeks, respectively. By testing a series of naturally occurring viruses as well as Delta-Omicron chimeric replicons, we show that Omicron nonstructural protein 6 harbors critical attenuating mutations, which dampen viral RNA replication and reduce lipid droplet consumption. Thus, pGLUE overcomes remaining barriers to broadly study SARS-CoV-2 replication and reveals deficits in nonstructural protein function underlying Omicron attenuation.
Language	English
Publishing date	2023-02-09
Publishing country	United States
Document type	Preprint
DOI	10.1101/2023.01.31.525914
Database	MEDical Literature Analysis and Retrieval System OnLINE

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article: The Regulation of HBV Transcription and Replication.

Oropeza, Claudia E / Tarnow, Grant / Sridhar, Abhayavarshini / Taha, Taha Y / Shalaby, Rasha E / McLachlan, Alan

Advances in experimental medicine and biology

2019 Volume 1179, Page(s) 39–69

Abstract: Hepatitis B virus (HBV) is a major human pathogen lacking a reliable curative therapy. Current therapeutics target the viral reverse transcriptase/DNA polymerase to inhibit viral replication but generally fail to resolve chronic HBV infections. Due to ... ...

Abstract	Hepatitis B virus (HBV) is a major human pathogen lacking a reliable curative therapy. Current therapeutics target the viral reverse transcriptase/DNA polymerase to inhibit viral replication but generally fail to resolve chronic HBV infections. Due to the limited coding potential of the HBV genome, alternative approaches for the treatment of chronic infections are desperately needed. An alternative approach to the development of antiviral therapeutics is to target cellular gene products that are critical to the viral life cycle. As transcription of the viral genome is an essential step in the viral life cycle, the selective inhibition of viral RNA synthesis is a possible approach for the development of additional therapeutic modalities that might be used in combination with currently available therapies. To address this possibility, a molecular understanding of the relationship between viral transcription and replication is required. The first step is to identify the transcription factors that are the most critical in controlling the levels of HBV RNA synthesis and to determine their in vivo role in viral biosynthesis. Mapping studies in cell culture utilizing reporter gene constructs permitted the identification of both ubiquitous and liver-enriched transcription factors capable of modulating transcription from the four HBV promoters. However, it was challenging to determine their relative importance for viral biosynthesis in the available human hepatoma replication systems. This technical limitation was addressed, in part, by the development of non-hepatoma HBV replication systems where viral biosynthesis was dependent on complementation with exogenously expressed transcription factors. These systems revealed the importance of specific nuclear receptors and hepatocyte nuclear factor 3 (HNF3)/forkhead box A (FoxA) transcription factors for HBV biosynthesis. Furthermore, using the HBV transgenic mouse model of chronic viral infection, the importance of various nuclear receptors and FoxA isoforms could be established in vivo. The availability of this combination of systems now permits a rational approach toward the development of selective host transcription factor inhibitors. This might permit the development of a new class of therapeutics to aid in the treatment and resolution of chronic HBV infections, which currently affects approximately 1 in 30 individuals worldwide and kills up to a million people annually.
MeSH term(s)	Animals ; DNA, Viral/chemistry ; Hepatitis B virus/genetics ; Hepatitis B virus/physiology ; Humans ; Mice ; Transcription, Genetic/genetics ; Virus Replication/genetics
Chemical Substances	DNA, Viral
Language	English
Publishing date	2019-11-18
Publishing country	United States
Document type	Journal Article ; Review
ISSN	2214-8019 ; 0065-2598
ISSN (online)	2214-8019
ISSN	0065-2598
DOI	10.1007/978-981-13-9151-4_3
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Relative DNA Methylation and Demethylation Efficiencies during Postnatal Liver Development Regulate Hepatitis B Virus Biosynthesis.

Oropeza, Claudia E / Tarnow, Grant / Taha, Taha Y / Shalaby, Rasha E / Hyde, Marieta V / Maienschein-Cline, Mark / Green, Stefan J / McLachlan, Alan

Journal of virology

2021 Volume 95, Issue 6

Abstract: Hepatitis B virus (HBV) transcription and replication increase progressively throughout postnatal liver development with maximal viral biosynthesis occurring at around 4 weeks of age in the HBV transgenic mouse model of chronic infection. Increasing ... ...

Abstract	Hepatitis B virus (HBV) transcription and replication increase progressively throughout postnatal liver development with maximal viral biosynthesis occurring at around 4 weeks of age in the HBV transgenic mouse model of chronic infection. Increasing viral biosynthesis is associated with a corresponding progressive loss of DNA methylation. The loss of DNA methylation is associated with increasing levels of 5-hydroxymethylcytosine (5hmC) residues which correlate with increased liver-enriched pioneer transcription factor Forkhead box protein A (FoxA) RNA levels, a rapid decline in postnatal liver DNA methyltransferase (Dnmt) transcripts, and a very modest reduction in ten-eleven translocation (Tet) methylcytosine dioxygenase expression. These observations are consistent with the suggestion that the balance between active HBV DNA methylation and demethylation is regulated by FoxA recruitment of Tet in the presence of declining Dnmt activity. These changes lead to demethylation of the viral genome during hepatocyte maturation with associated increases in viral biosynthesis. Consequently, manipulation of the relative activities of these two counterbalancing processes might permit the specific silencing of HBV gene expression with the loss of viral biosynthesis and the resolution of chronic HBV infections.
MeSH term(s)	5-Methylcytosine/analogs & derivatives ; 5-Methylcytosine/metabolism ; Animals ; Animals, Newborn ; DNA (Cytosine-5-)-Methyltransferases/genetics ; DNA (Cytosine-5-)-Methyltransferases/metabolism ; DNA Methylation ; DNA Replication ; DNA, Viral/biosynthesis ; DNA, Viral/metabolism ; Demethylation ; Dioxygenases/genetics ; Dioxygenases/metabolism ; Disease Models, Animal ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Viral ; Hepatitis B virus/physiology ; Hepatitis B, Chronic/metabolism ; Hepatitis B, Chronic/pathology ; Hepatitis B, Chronic/virology ; Hepatocyte Nuclear Factors/genetics ; Hepatocyte Nuclear Factors/metabolism ; Liver/growth & development ; Liver/metabolism ; Liver/virology ; Mice ; Mice, Transgenic ; RNA, Viral/biosynthesis ; Virus Replication
Chemical Substances	DNA, Viral ; Hepatocyte Nuclear Factors ; RNA, Viral ; 5-hydroxymethylcytosine (1123-95-1) ; 5-Methylcytosine (6R795CQT4H) ; Dioxygenases (EC 1.13.11.-) ; Tet3 protein, mouse (EC 1.13.11.-) ; DNA (Cytosine-5-)-Methyltransferases (EC 2.1.1.37)
Language	English
Publishing date	2021-02-24
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	80174-4
ISSN	1098-5514 ; 0022-538X
ISSN (online)	1098-5514
ISSN	0022-538X
DOI	10.1128/JVI.02148-20
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 609: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Scaffold dependent histone deacetylase (HDAC) inhibitor induced re-equilibration of the subcellular localization and post-translational modification state of class I HDACs.

Hanigan, Thomas W / Taha, Taha Y / Aboukhatwa, Shaimaa M / Frasor, Jonna / Petukhov, Pavel A

PloS one

2017 Volume 12, Issue 10, Page(s) e0186620

Abstract: The mechanism of action of histone deacetylase inhibitors (HDACi) is mainly attributed to the inhibition of the deacetylase catalytic activity for their histone substrates. In this study, we analyzed the abundance of class I HDACs in the cytosolic, ... ...

Abstract	The mechanism of action of histone deacetylase inhibitors (HDACi) is mainly attributed to the inhibition of the deacetylase catalytic activity for their histone substrates. In this study, we analyzed the abundance of class I HDACs in the cytosolic, nuclear soluble and chromatin bound cellular fractions in breast cancer cells after HDACi treatment. We found that potent N-hydroxy propenamide-based HDACi induced a concentration dependent decrease in the HDAC1 associated with chromatin and a lasting concomitant increase in cytoplasmic HDAC1 while maintaining total protein expression. No such change occurred with HDAC2 or 8, however, an increase in cytoplasmic non-phosphorylated HDAC3 was also observed. The subcellular re-equilibration of HDAC1 was subsequent to the accumulation of acetylated histones and might be cell cycle dependent. This study suggests that the biological activity of a subset of N-hydroxy propenamide-based HDACi may stem from direct competition with histone substrates of HDACs as well as from spatial separation from their substrates in the nucleus and/or change in post-translational modification status of HDACs.
MeSH term(s)	Acetylation/drug effects ; Cytosol/drug effects ; Cytosol/enzymology ; Histone Deacetylase Inhibitors/chemistry ; Histone Deacetylase Inhibitors/pharmacology ; Histone Deacetylases/metabolism ; Histones/metabolism ; Humans ; MCF-7 Cells ; Microscopy, Confocal ; Mitogens/pharmacology ; Models, Biological ; Phosphorylation/drug effects ; Protein Processing, Post-Translational/drug effects ; Protein Transport/drug effects ; Subcellular Fractions/drug effects ; Subcellular Fractions/enzymology
Chemical Substances	Histone Deacetylase Inhibitors ; Histones ; Mitogens ; Histone Deacetylases (EC 3.5.1.98)
Language	English
Publishing date	2017-10-18
Publishing country	United States
Document type	Journal Article
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0186620
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED