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  1. Article ; Online: Effect of protamine and Its abnormalities on male fertility potential

    Tahereh Rahiminia / Javad Amini Mahabadi / Ehsan Farashahi Yazd / Ali Reza Talebi

    مجله علمی دانشگاه علوم پزشکی کردستان, Vol 25, Iss 5, Pp 52-

    2020  Volume 66

    Abstract: ABSTRACT Background and Aim: All studies on mammals and rodents have revealed the presence of protamine 1 (P1) and protamine 2 (P2) in the sperm which is indicative of the expression of these two genes at different molecular levels. The aim of this study ...

    Abstract ABSTRACT Background and Aim: All studies on mammals and rodents have revealed the presence of protamine 1 (P1) and protamine 2 (P2) in the sperm which is indicative of the expression of these two genes at different molecular levels. The aim of this study was to investigate the effect of protamine and its disorders on male fertility potential. Materials and Methods: Using keywords of sperm, protamine, male infertility, and chromatin we searched PubMed and Google Scholar databases between 1980 and 2020. Results: Sperm concentration, motility, and morphology in the patients with variable P1/P2 ratios were significantly reduced compared to those in the individuals with normal P1/P2 ratios which were directly associated with reduced fertility rate. The most common protamine abnormality in the infertile men was increased P1/P2 ratio which was frequently associated with a decreased level of P2 and increased level of P2 precursors. Increased levels of histone B2 (H2B) in sperm and lower levels of protamine have been reported. Any disturbances in the histone expression process cause inconvenient early chromatin condensation, transcription arrest, as well as spermatogenesis failures. Conclusion: The results of this study showed that the protamine transcripts ratio can be used as a marker for male fertility. Histones/protamines mRNAs ratios are important for sperm quality and therefore can be used as predictors for male infertility. Altered levels of protamines may result in an increased susceptibility to injury in the sperm DNA causing infertility or poor outcome in assisted reproduction.
    Keywords male infertility ; sperm ; protamine ; chromatin ; Medicine ; R ; Medicine (General) ; R5-920
    Language Persian
    Publishing date 2020-11-01T00:00:00Z
    Publisher Kurdistan University of Medical Sciences
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Cumulus co-culture system does not improve the in-vitro maturation (IVM) of oocytes in mice

    Tahereh Rahiminia / Azita Faramarzi / Arezoo Khoradmehr / Mohammad Ali Khalili

    Middle East Fertility Society Journal, Vol 21, Iss 2, Pp 109-

    2016  Volume 113

    Abstract: Objective: To examine the effect of co-culture system with CCs on the in vitro maturation (IVM) of immature oocytes retrieved from mice. Design: This is an experimental study. Setting: Research and Clinical Center for Infertility of Yazd. Sample: A total ...

    Abstract Objective: To examine the effect of co-culture system with CCs on the in vitro maturation (IVM) of immature oocytes retrieved from mice. Design: This is an experimental study. Setting: Research and Clinical Center for Infertility of Yazd. Sample: A total of 144 oocytes from 5 to 6 weeks mice. Material: After dissecting ovaries, denuded GVs (DGVs) were considered as control group and put in IVM medium. In cumulus oocyte complexes (COCs) as exp I, intact COCs were put in IVM medium and observed for maturation after 24 h and 48 h. In exp II, GVs were co-cultured with mature CCs. For exp II, COCs were incubated for 30 min before removing oocytes. Then, denuded mature oocytes were removed and the remaining CCs were used to culture denuded GVs recovered from ovaries. Main outcome measures: Maturation rates of oocytes after 24 h and 48 h. Results: The rate of oocyte maturation was 77.36 ± 14.4 in control group. In exp I and exp II, the rates of maturation were 91.32 ± 22.5 and 63.33 ± 7.4, respectively (P = 0.04). However, maturation rate did not increase after 24 h. The maturation rate in metaphase I (MI) oocytes was as low as 15% in different groups at 24 h and did not increase by 48 h. Degeneration rate was increased from time 0 to 48 h, but not significant in different times. Conclusion: Presence of intact COCs significantly improved the oocyte maturation rates in animal model. However, co-culture of GVs with mature CCs did not improve IVM program in mice.
    Keywords In vitro maturation ; Cumulus cells ; Mice ; Medicine (General) ; R5-920 ; Reproduction ; QH471-489
    Subject code 570
    Language English
    Publishing date 2016-06-01T00:00:00Z
    Publisher SpringerOpen
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Etiologies of sperm oxidative stress

    Parvin Sabeti / Soheila Pourmasumi / Tahereh Rahiminia / Fatemeh Akyash / Ali Reza Talebi

    Iranian Journal of Reproductive Medicine, Vol 14, Iss 4, Pp 231-

    2016  Volume 240

    Abstract: Sperm is particularly susceptible to reactive oxygen species (ROS) during critical phases of spermiogenesis. However, the level of seminal ROS is restricted by seminal antioxidants which have beneficial effects on sperm parameters and developmental ... ...

    Abstract Sperm is particularly susceptible to reactive oxygen species (ROS) during critical phases of spermiogenesis. However, the level of seminal ROS is restricted by seminal antioxidants which have beneficial effects on sperm parameters and developmental potentials. Mitochondria and sperm plasma membrane are two major sites of ROS generation in sperm cells. Besides, leukocytes including polymer phonuclear (PMN) leukocytes and macrophages produce broad category of molecules including oxygen free radicals, non-radical species and reactive nitrogen species. Physiological role of ROS increase the intracellular cAMP which then activate protein kinase in male reproductive system. This indicates that spermatozoa need small amounts of ROS to acquire the ability of nuclear maturation regulation and condensation to fertilize the oocyte. There is a long list of intrinsic and extrinsic factors which can induce oxidative stress to interact with lipids, proteins and DNA molecules. As a result, we have lipid peroxidation, DNA fragmentation, axonemal damage, denaturation of the enzymes, over generation of superoxide in the mitochondria, lower antioxidant activity and finally abnormal spermatogenesis. If oxidative stress is considered as one of the main cause of DNA damage in the germ cells, then there should be good reason for antioxidant therapy in these conditions
    Keywords Medicine ; R ; Gynecology and obstetrics ; RG1-991 ; Reproduction ; QH471-489
    Subject code 572
    Language English
    Publishing date 2016-04-01T00:00:00Z
    Publisher Shahid Sadoughi University of Medical Science, Yazd, Iran
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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