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  1. Article: Exposure to high concentrations of carbon dioxide during transporting a cadaver preserved with dry ice inside an ambulance vehicle.

    Oyama, Ibuki / Tajima, Yasuhisa / Ojima, Toshiyuki / Iida, Akiyoshi

    Forensic toxicology

    2022  Volume 41, Issue 1, Page(s) 179–182

    MeSH term(s) Humans ; Dry Ice ; Carbon Dioxide/analysis ; Ambulances ; Preservation, Biological ; Cadaver
    Chemical Substances Dry Ice ; Carbon Dioxide (142M471B3J)
    Language English
    Publishing date 2022-10-05
    Publishing country Japan
    Document type Letter
    ZDB-ID 2274095-8
    ISSN 1860-8965
    ISSN 1860-8965
    DOI 10.1007/s11419-022-00644-8
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    Zs.A 3901: Show issues Location:
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  2. Article ; Online: Diagnostic Method for COVID-19 Using Sugar Chain-Immobilized Nanoparticles and Saliva Specimens.

    Suda, Yasuo / Tajima, Yasuhisa / Nishi, Jun-Ichiro / Kajiya, Takashi

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2452, Page(s) 63–74

    Abstract: Identification of viruses that infects animals or plants, and determination of their quantity are essential for the diagnosis of infectious disease and for the determination of a strategy in the treatment of virus-derived diseases. However, the ... ...

    Abstract Identification of viruses that infects animals or plants, and determination of their quantity are essential for the diagnosis of infectious disease and for the determination of a strategy in the treatment of virus-derived diseases. However, the concentration of viruses existing in a living body (in bodily fluid), food, drinking water, river water, and so on. is not high enough to be detected using conventional diagnostic methods. For example, since the concentration of influenza virus released from an infected person is less than the detection limit of conventional simple examination kits (rapid kit) or even a PCR process at the initial stage of infection, it is difficult to detect the presence of influenza virus which will lead to influenza disease. Our technology allows for safe, efficient, and selective concentration of viruses without troublesome ultracentrifugation, using sugar chain-immobilized metal nanoparticles based on the binding interaction between viruses and sugar chains. For COVID-19, we have developed and commercialized two molecular diagnosis kits: SUDx SARS-CoV-2 detection kit, and SGNP nCoV/Flu PCR detection kit, for the Japanese market in 2020.
    MeSH term(s) Animals ; COVID-19/diagnosis ; Humans ; Influenza, Human/diagnosis ; Nanoparticles ; SARS-CoV-2/genetics ; Saliva ; Sensitivity and Specificity ; Sugars
    Chemical Substances Sugars
    Language English
    Publishing date 2022-05-12
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2111-0_5
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  3. Article ; Online: A case report of SARS-CoV-2 confirmed in saliva specimens up to 37 days after onset: Proposal of saliva specimens for COVID-19 diagnosis and virus monitoring.

    Tajima, Yasuhisa / Suda, Yasuo / Yano, Kunio

    Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy

    2020  Volume 26, Issue 10, Page(s) 1086–1089

    Abstract: We present the case of a 71-year-old man who, despite becoming asymptomatic after having some mild symptoms of COVID-19, had SARS-CoV-2 RNA detected for 37 days after onset, from his concentrated and purified saliva specimens using sugar chain- ... ...

    Abstract We present the case of a 71-year-old man who, despite becoming asymptomatic after having some mild symptoms of COVID-19, had SARS-CoV-2 RNA detected for 37 days after onset, from his concentrated and purified saliva specimens using sugar chain-immobilized gold nanoparticles. It was suggested that the early morning saliva specimens were more likely to show positive results than those obtained later in the day.
    MeSH term(s) Aged ; Betacoronavirus/isolation & purification ; COVID-19 ; COVID-19 Testing ; Clinical Laboratory Techniques ; Coronavirus Infections/diagnosis ; Coronavirus Infections/virology ; Gold/chemistry ; Humans ; Male ; Metal Nanoparticles ; Pandemics ; Pneumonia, Viral/diagnosis ; Pneumonia, Viral/virology ; RNA, Viral/isolation & purification ; Real-Time Polymerase Chain Reaction ; SARS-CoV-2 ; Saliva/virology ; Time Factors ; Viral Load ; Virus Shedding
    Chemical Substances RNA, Viral ; Gold (7440-57-5)
    Keywords covid19
    Language English
    Publishing date 2020-06-13
    Publishing country Netherlands
    Document type Case Reports
    ZDB-ID 1355399-9
    ISSN 1437-7780 ; 1341-321X
    ISSN (online) 1437-7780
    ISSN 1341-321X
    DOI 10.1016/j.jiac.2020.06.011
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    Zs.A 5236: Show issues Location:
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  4. Article ; Online: Coronavirus hunted in human pneumocytes and alveolar macrophages: a case report.

    Kataoka, Michiyo / Tsukamoto, Tetsuya / Tajima, Yasuhisa / Sato, Yuko / Katano, Harutaka / Suzuki, Tadaki / Nakajima, Noriko

    Histopathology

    2022  Volume 80, Issue 7, Page(s) 1130–1134

    MeSH term(s) Alveolar Epithelial Cells ; Coronavirus ; Coronavirus Infections ; Humans ; Lung ; Macrophages, Alveolar ; Pulmonary Alveoli
    Language English
    Publishing date 2022-03-31
    Publishing country England
    Document type Case Reports ; Journal Article
    ZDB-ID 131914-0
    ISSN 1365-2559 ; 0309-0167
    ISSN (online) 1365-2559
    ISSN 0309-0167
    DOI 10.1111/his.14637
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    Zs.A 1328: Show issues Location:
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  5. Article ; Online: The effect of 1-hydroxy-vitamin D treatment in hospitalized patients with COVID-19: A retrospective study.

    Ogasawara, Takashi / Tajima, Yasuhisa / Nakamura, Naoto / Kanasaki, Hiroki / Matsuyama, Wataru / Niwa, Mitsuru / Ozawa, Yuichi / Sugiura, Masayuki / Ogiku, Masahito / Sato, Jun

    Clinical nutrition (Edinburgh, Scotland)

    2023  Volume 42, Issue 10, Page(s) 2045–2050

    Abstract: Background & aims: The efficacy of vitamin D supplementation in coronavirus disease 2019 (COVID-19) remains unclear. This study aimed to evaluate the effect of 1-hydroxy-vitamin D on the prevention of severe disease and mortality in patients ... ...

    Abstract Background & aims: The efficacy of vitamin D supplementation in coronavirus disease 2019 (COVID-19) remains unclear. This study aimed to evaluate the effect of 1-hydroxy-vitamin D on the prevention of severe disease and mortality in patients hospitalized for COVID-19.
    Methods: This retrospective study included 312 patients with COVID-19 who were admitted to our hospital between April 2021 and October 2021 (primarily the Delta variant) and between July 2022 and September 2022 (primarily Omicron variant). Serum 25-hydroxyvitamin D (25(OH)D) levels were measured at the time of admission and 1-hydroxy-vitamin D was prescribed by the treating physicians. The patients were divided into two groups: those administered 1-hydroxy-vitamin D (Vit D group) and those who were not (control group). The composite primary endpoint was the need for additional respiratory support, including high-flow oxygen therapy or invasive mechanical ventilation, and in-hospital mortality rate.
    Results: Of 312 patients, 122 (39%) received 1-hydroxy-vitamin D treatment. Although the median age was not significantly higher in the Vit D group than in the control group (66 vs. 58 years old, P = 0.06) and there was no significant difference in the proportion of vitamin D deficiency (defined as serum 25(OH)D level less than 20 ng/mL, 77% vs. 65%, P = 0.07), patients in the control group had a more severe baseline profile compared to the Vit D group according to the Japanese disease severity definition for COVID-19 (P = 0.01). The proportion of those requiring more respiratory support and in-hospital mortality was significantly lower in the Vit D group than in the control group (6% vs. 14%, P = 0.01 log-rank test). After propensity score matching, a statistically significant difference in the primary endpoint was observed (P = 0.03 log-rank test).
    Conclusions: 1-hydroxy-vitamin treatment may improve outcomes in hospitalized patients with COVID-19, reducing composite outcomes including the need for additional respiratory support and in-hospital mortality.
    MeSH term(s) Humans ; Middle Aged ; COVID-19/blood ; COVID-19/complications ; COVID-19/mortality ; COVID-19/therapy ; Retrospective Studies ; SARS-CoV-2 ; Vitamin D/analogs & derivatives ; Vitamin D/blood ; Vitamin D/therapeutic use ; Vitamin D Deficiency/blood ; Vitamin D Deficiency/complications ; Vitamin D Deficiency/drug therapy ; Vitamins/therapeutic use ; Hydroxycholecalciferols/therapeutic use ; Aged ; Respiratory Insufficiency/etiology ; Respiratory Insufficiency/therapy ; Hospital Mortality
    Chemical Substances Vitamin D (1406-16-2) ; Vitamins ; Hydroxycholecalciferols ; 25-hydroxyvitamin D (A288AR3C9H)
    Language English
    Publishing date 2023-09-01
    Publishing country England
    Document type Journal Article
    ZDB-ID 604812-2
    ISSN 1532-1983 ; 0261-5614
    ISSN (online) 1532-1983
    ISSN 0261-5614
    DOI 10.1016/j.clnu.2023.08.021
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 1774: Show issues Location:
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  6. Article: A case report of SARS-CoV-2 confirmed in saliva specimens up to 37 days after onset: Proposal of saliva specimens for COVID-19 diagnosis and virus monitoring

    Tajima, Yasuhisa / Suda, Yasuo / Yano, Kunio

    J Infect Chemother

    Abstract: We present the case of a 71-year-old man who, despite becoming asymptomatic after having some mild symptoms of COVID-19, had SARS-CoV-2 RNA detected for 37 days after onset, from his concentrated and purified saliva specimens using sugar chain- ... ...

    Abstract We present the case of a 71-year-old man who, despite becoming asymptomatic after having some mild symptoms of COVID-19, had SARS-CoV-2 RNA detected for 37 days after onset, from his concentrated and purified saliva specimens using sugar chain-immobilized gold nanoparticles. It was suggested that the early morning saliva specimens were more likely to show positive results than those obtained later in the day.
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #597876
    Database COVID19

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  7. Article ; Online: A case report of SARS-CoV-2 confirmed in saliva specimens up to 37 days after onset

    Tajima, Yasuhisa / Suda, Yasuo / Yano, Kunio

    Journal of Infection and Chemotherapy

    Proposal of saliva specimens for COVID-19 diagnosis and virus monitoring

    2020  Volume 26, Issue 10, Page(s) 1086–1089

    Keywords Microbiology (medical) ; Pharmacology (medical) ; Infectious Diseases ; covid19
    Language English
    Publisher Elsevier BV
    Publishing country us
    Document type Article ; Online
    ZDB-ID 1355399-9
    ISSN 1437-7780 ; 1341-321X
    ISSN (online) 1437-7780
    ISSN 1341-321X
    DOI 10.1016/j.jiac.2020.06.011
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  8. Article ; Online: Three-year prospective, observational study of central line-associated bloodstream infections in a 600-bed Japanese acute care hospital.

    Matsui, Yasuko / Shimatani, Michitsugu / Kuzuhara, Kenta / Miyazaki, Yoshiko / Horiuchi, Tomoko / Tajima, Yasuhisa / Yano, Kunio / Nagata, Toshi

    American journal of infection control

    2015  Volume 43, Issue 5, Page(s) 494–498

    Abstract: Background: Central line-associated bloodstream infection (CLABSI) is an important concern associated with central venous catheter (CVC) use. The objective of this study was to determine the influences of CVC access sites, CVC types, and presumed ... ...

    Abstract Background: Central line-associated bloodstream infection (CLABSI) is an important concern associated with central venous catheter (CVC) use. The objective of this study was to determine the influences of CVC access sites, CVC types, and presumed causative microorganisms on CLABSI occurrence in an acute care hospital.
    Methods: We conducted a prospective, observational study of CLABSI occurrence for 3 consecutive years in a 600-bed Japanese acute care hospital. Data collected included patient characteristics, CVC access sites, CVC types, and microorganisms isolated by blood culture.
    Results: For 1,650 CVCs used for 1,237 patients, 39 cases of infection were identified. Most infections had occurred within 1 month of CVC insertion. Maximal sterile barrier precautions had been used for most cases (97.3%). The average CLABSI occurrence days with internal jugular vein access were shorter than those with subclavian vein access and femoral vein access. CLABSI rates were 1.1 and 0.7 for single- and multilumen CVCs, respectively. CLABSI occurrence tended to be shorter when gram-positive cocci were isolated and tended to be longer when fungi (Candida spp) were isolated.
    Conclusion: Most CLABSI cases had occurred within 1 month of CVC insertion. Longer CVC duration increased chance of fungal infection.
    MeSH term(s) Adolescent ; Adult ; Aged ; Aged, 80 and over ; Bacteria/classification ; Bacteria/isolation & purification ; Catheter-Related Infections/epidemiology ; Catheterization, Central Venous/adverse effects ; Female ; Fungi/classification ; Fungi/isolation & purification ; Hospitals ; Humans ; Japan ; Male ; Middle Aged ; Prospective Studies ; Sepsis/epidemiology ; Young Adult
    Language English
    Publishing date 2015-05-01
    Publishing country United States
    Document type Journal Article ; Observational Study
    ZDB-ID 392362-9
    ISSN 1527-3296 ; 0196-6553
    ISSN (online) 1527-3296
    ISSN 0196-6553
    DOI 10.1016/j.ajic.2015.01.011
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