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  1. Article ; Online: Cultivation of Microorganisms in Media Supplemented with Mucin Glycoproteins.

    Takada, Hiromi / Katayama, Takane / Katoh, Toshihiko

    Methods in molecular biology (Clifton, N.J.)

    2024  Volume 2763, Page(s) 331–335

    Abstract: To examine the mucin-utilizing capacity of bacterial isolates from fecal samples, an in vitro cultivation method using mucins as a carbon source should be considered. This chapter describes a practical method for cultivating bacteria in media containing ... ...

    Abstract To examine the mucin-utilizing capacity of bacterial isolates from fecal samples, an in vitro cultivation method using mucins as a carbon source should be considered. This chapter describes a practical method for cultivating bacteria in media containing mucin glycoproteins; for this cultivation method, several factors are considered due to the physical nature of mucin glycoproteins.
    MeSH term(s) Mucins ; Glycoproteins ; Bacteria
    Chemical Substances Mucins ; Glycoproteins
    Language English
    Publishing date 2024-02-13
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3670-1_27
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  2. Article ; Online: Fructooligosaccharides Intake during Pregnancy Improves Metabolic Phenotype of Offspring in High Fat Diet-Induced Obese Mice.

    Miyamoto, Junki / Ando, Yuna / Nishida, Akari / Yamano, Mayu / Suzuki, Shunsuke / Takada, Hiromi / Kimura, Ikuo

    Molecular nutrition & food research

    2024  , Page(s) e2300758

    Abstract: Scope: Obesity and metabolic diseases are closely associated, and individuals who become obese are also prone to type 2 diabetes and cardiovascular disorders. Gut microbiota is mediated by diet and can influence host metabolism and the incidence of ... ...

    Abstract Scope: Obesity and metabolic diseases are closely associated, and individuals who become obese are also prone to type 2 diabetes and cardiovascular disorders. Gut microbiota is mediated by diet and can influence host metabolism and the incidence of metabolic disorders. Recent studies have suggested that improving gut microbiota through a fructooligosaccharide (FOS)-supplemented diet may ameliorate obesity and other metabolic disorders. Although accumulating evidence supports the notion of the developmental origins of health and disease, the underlying mechanisms remain obscure.
    Methods and results: ICR mice are fed AIN-93G formula-based cellulose -, FOS-, acetate-, or propionate-supplemented diets during pregnancy. Offspring are reared by conventional ICR foster mothers for 4 weeks; weaned mice are fed high fat diet for 12 weeks and housed individually. The FOS and propionate offspring contribute to suppressing obesity and improving glucose intolerance. Gut microbial compositions in FOS-fed mothers and their offspring are markedly changed. However, the beneficial effect of FOS diet on the offspring is abolished when antibiotics are administered to pregnant mice.
    Conclusion: The findings highlight the link between the maternal gut environment and the developmental origin of metabolic syndrome in offspring. These results open novel research avenues into preemptive therapies for metabolic disorders by targeting the maternal gut microbiota.
    Language English
    Publishing date 2024-04-19
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2160372-8
    ISSN 1613-4133 ; 1613-4125
    ISSN (online) 1613-4133
    ISSN 1613-4125
    DOI 10.1002/mnfr.202300758
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  3. Article ; Online: GH20 and GH84 β-N-acetylglucosaminidases with different linkage specificities underpin mucin O-glycan breakdown capability of Bifidobacterium bifidum.

    Takada, Hiromi / Katoh, Toshihiko / Sakanaka, Mikiyasu / Odamaki, Toshitaka / Katayama, Takane

    The Journal of biological chemistry

    2023  Volume 299, Issue 6, Page(s) 104781

    Abstract: Intestinal mucous layers mediate symbiosis and dysbiosis of host-microbe interactions. These interactions are influenced by the mucin O-glycan degrading ability of several gut microbes. The identities and prevalence of many glycoside hydrolases (GHs) ... ...

    Abstract Intestinal mucous layers mediate symbiosis and dysbiosis of host-microbe interactions. These interactions are influenced by the mucin O-glycan degrading ability of several gut microbes. The identities and prevalence of many glycoside hydrolases (GHs) involved in microbial mucin O-glycan breakdown have been previously reported; however, the exact mechanisms and extent to which these GHs are dedicated to mucin O-glycan degradation pathways warrant further research. Here, using Bifidobacterium bifidum as a model mucinolytic bacterium, we revealed that two β-N-acetylglucosaminidases belonging to the GH20 (BbhI) and GH84 (BbhIV) families play important roles in mucin O-glycan degradation. Using substrate specificity analysis of natural oligosaccharides and O-glycomic analysis of porcine gastric mucin (PGM) incubated with purified enzymes or B. bifidum carrying bbhI and/or bbhIV mutations, we showed that BbhI and BbhIV are highly specific for β-(1→3)- and β-(1→6)-GlcNAc linkages of mucin core structures, respectively. Interestingly, we found that efficient hydrolysis of the β-(1→3)-linkage by BbhI of the mucin core 4 structure [GlcNAcβ1-3(GlcNAcβ1-6)GalNAcα-O-Thr] required prior removal of the β-(1→6)-GlcNAc linkage by BbhIV. Consistent with this, inactivation of bbhIV markedly decreased the ability of B. bifidum to release GlcNAc from PGM. When combined with a bbhI mutation, we observed that the growth of the strain on PGM was reduced. Finally, phylogenetic analysis suggests that GH84 members may have gained diversified functions through microbe-microbe and host-microbe horizontal gene transfer events. Taken together, these data strongly suggest the involvement of GH84 family members in host glycan breakdown.
    MeSH term(s) Animals ; Acetylglucosaminidase/chemistry ; Acetylglucosaminidase/metabolism ; Bacterial Proteins/metabolism ; Bifidobacterium bifidum/classification ; Bifidobacterium bifidum/enzymology ; Bifidobacterium bifidum/genetics ; Mucins/metabolism ; Phylogeny ; Swine
    Chemical Substances Acetylglucosaminidase (EC 3.2.1.52) ; Bacterial Proteins ; Mucins
    Language English
    Publishing date 2023-05-03
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2997-x
    ISSN 1083-351X ; 0021-9258
    ISSN (online) 1083-351X
    ISSN 0021-9258
    DOI 10.1016/j.jbc.2023.104781
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  4. Article ; Online: Sialylated

    Takada, Hiromi / Katoh, Toshihiko / Katayama, Takane

    Journal of applied glycoscience

    2020  Volume 67, Issue 2, Page(s) 31–39

    Abstract: Ovomucin, a hen egg white protein, is characterized by its hydrogel-forming properties, high molecular weight, and ... ...

    Abstract Ovomucin, a hen egg white protein, is characterized by its hydrogel-forming properties, high molecular weight, and extensive
    Language English
    Publishing date 2020-05-20
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 2395554-5
    ISSN 1880-7291 ; 1344-7882
    ISSN (online) 1880-7291
    ISSN 1344-7882
    DOI 10.5458/jag.jag.JAG-2019_0020
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  5. Article ; Online: Walking aids and complicated orthopedic diseases are risk factors for falls in hemodialysis patients: an observational study.

    Ishii, Takeo / Matsumoto, Wataru / Hoshino, Yui / Kagawa, Yasuhiro / Iwasaki, Emi / Takada, Hiromi / Honma, Takashi / Oyama, Kunio

    BMC geriatrics

    2023  Volume 23, Issue 1, Page(s) 319

    Abstract: Background: Aging and an increased fall risk have been demonstrated in hemodialysis patients at home and in a facility. However, studies investigating the cause of falls to prevent fractures in dialysis rooms are scarce. This study aimed to explore the ... ...

    Abstract Background: Aging and an increased fall risk have been demonstrated in hemodialysis patients at home and in a facility. However, studies investigating the cause of falls to prevent fractures in dialysis rooms are scarce. This study aimed to explore the related factors for accidental falls statistically in dialysis facilities for future fall prevention.
    Methods: This study included 629 hemodialysis patients with end-stage renal disease. The patients were divided into two groups: the fall and non-fall groups. The main outcome was the presence or absence of falls in the dialysis room. Univariate and multivariate logistic analyses were performed; multivariate analysis was conducted using covariates significantly correlated in the univariate analysis.
    Results: A total of 133 patients experienced falling accidents during the study period. The multivariate analysis indicated that the use of walking aid (p < 0.001), orthopedic diseases (p < 0.05), cerebrovascular disease, and age were significantly correlated with falls.
    Conclusions: In the dialysis clinic, patients who use walking aids and have complicated orthopedic or cerebrovascular conditions are at a high risk of falling in the dialysis room. Therefore, establishing a safe environment may help prevent falls, not only for these patients but also among other patients with similar conditions.
    MeSH term(s) Humans ; Risk Factors ; Renal Dialysis/adverse effects ; Kidney Failure, Chronic/epidemiology ; Kidney Failure, Chronic/therapy ; Walking ; Ambulatory Care Facilities
    Language English
    Publishing date 2023-05-22
    Publishing country England
    Document type Observational Study ; Journal Article
    ZDB-ID 2059865-8
    ISSN 1471-2318 ; 1471-2318
    ISSN (online) 1471-2318
    ISSN 1471-2318
    DOI 10.1186/s12877-023-04015-9
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  6. Article ; Online: How COVID-19 Affected the Introduction of Telemedicine and Patient Reported Outcomes Among Patients With Pulmonary Hypertension　- A Report From a Referral Center in Japan.

    Tamura, Yuichi / Takeyasu, Rika / Furukawa, Asuka / Takada, Hiromi / Takechi, Mineki / Taniguchi, Hirohisa / Kawamura, Akio

    Circulation reports

    2020  Volume 2, Issue 9, Page(s) 526–530

    Abstract: Background: ...

    Abstract Background:
    Language English
    Publishing date 2020-08-27
    Publishing country Japan
    Document type Journal Article
    ISSN 2434-0790
    ISSN (online) 2434-0790
    DOI 10.1253/circrep.CR-20-0088
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  7. Article: Bifidobacterium bifidum

    Ojima, Miriam N / Gotoh, Aina / Takada, Hiromi / Odamaki, Toshitaka / Xiao, Jin-Zhong / Katoh, Toshihiko / Katayama, Takane

    Frontiers in microbiology

    2020  Volume 11, Page(s) 1349

    Abstract: The gut microbiome is a dynamic community that significantly affects host health; it is frequently disturbed by medications such as antibiotics. Recently, probiotics have been proposed as a remedy for antibiotic-induced dysbiosis, but the efficacy of ... ...

    Abstract The gut microbiome is a dynamic community that significantly affects host health; it is frequently disturbed by medications such as antibiotics. Recently, probiotics have been proposed as a remedy for antibiotic-induced dysbiosis, but the efficacy of such treatments remains uncertain. Thus, the effect of specific antibiotic-probiotic combinations on the gut microbiome and host health warrants further research. We tested the effect vancomycin, amoxicillin, and ciprofloxacin on mice. Antibiotic administration was followed by one of the following recovery treatments:
    Language English
    Publishing date 2020-06-18
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2020.01349
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  8. Article: How COVID-19 Affected the Introduction of Telemedicine and Patient Reported Outcomes Among Patients With Pulmonary Hypertension ― A Report From a Referral Center in Japan ―

    Tamura, Yuichi / Takeyasu, Rika / Furukawa, Asuka / Takada, Hiromi / Takechi, Mineki / Taniguchi, Hirohisa / Kawamura, Akio

    Circulation Reports

    Abstract: Abstract Background:COVID-19 is fatal to patients with pulmonary hypertension (PH), so preventive actions are recommended This study investigated the effectiveness of telemedicine and effects on quality of life (QOL) in the treatment of patients with PH ... ...

    Abstract Abstract Background:COVID-19 is fatal to patients with pulmonary hypertension (PH), so preventive actions are recommended This study investigated the effectiveness of telemedicine and effects on quality of life (QOL) in the treatment of patients with PH Methods and Results:Japanese patients with PH (n=40) were recruited from one referral center Patient self-reported anxiety worsened significantly and elderly patients in particular experienced detrimental lifestyle changes under COVID-19 Telemedicine worked well to decrease the frequency of going out Conclusions:Telemedicine is effective in reducing travel distances, and frequent remote interventions may be desirable for older, anxious patients
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #732224
    Database COVID19

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  9. Article ; Online: A bacterial sulfoglycosidase highlights mucin O-glycan breakdown in the gut ecosystem.

    Katoh, Toshihiko / Yamada, Chihaya / Wallace, Michael D / Yoshida, Ayako / Gotoh, Aina / Arai, Moe / Maeshibu, Takako / Kashima, Toma / Hagenbeek, Arno / Ojima, Miriam N / Takada, Hiromi / Sakanaka, Mikiyasu / Shimizu, Hidenori / Nishiyama, Keita / Ashida, Hisashi / Hirose, Junko / Suarez-Diez, Maria / Nishiyama, Makoto / Kimura, Ikuo /
    Stubbs, Keith A / Fushinobu, Shinya / Katayama, Takane

    Nature chemical biology

    2023  Volume 19, Issue 6, Page(s) 778–789

    Abstract: Mucinolytic bacteria modulate host-microbiota symbiosis and dysbiosis through their ability to degrade mucin O-glycans. However, how and to what extent bacterial enzymes are involved in the breakdown process remains poorly understood. Here we focus on a ... ...

    Abstract Mucinolytic bacteria modulate host-microbiota symbiosis and dysbiosis through their ability to degrade mucin O-glycans. However, how and to what extent bacterial enzymes are involved in the breakdown process remains poorly understood. Here we focus on a glycoside hydrolase family 20 sulfoglycosidase (BbhII) from Bifidobacterium bifidum, which releases N-acetylglucosamine-6-sulfate from sulfated mucins. Glycomic analysis showed that, in addition to sulfatases, sulfoglycosidases are involved in mucin O-glycan breakdown in vivo and that the released N-acetylglucosamine-6-sulfate potentially affects gut microbial metabolism, both of which were also supported by a metagenomic data mining analysis. Enzymatic and structural analysis of BbhII reveals the architecture underlying its specificity and the presence of a GlcNAc-6S-specific carbohydrate-binding module (CBM) 32 with a distinct sugar recognition mode that B. bifidum takes advantage of to degrade mucin O-glycans. Comparative analysis of the genomes of prominent mucinolytic bacteria also highlights a CBM-dependent O-glycan breakdown strategy used by B. bifidum.
    MeSH term(s) Mucins/metabolism ; Ecosystem ; Polysaccharides/metabolism ; Bacteria/metabolism
    Chemical Substances Mucins ; Polysaccharides
    Language English
    Publishing date 2023-03-02
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2202962-X
    ISSN 1552-4469 ; 1552-4450
    ISSN (online) 1552-4469
    ISSN 1552-4450
    DOI 10.1038/s41589-023-01272-y
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    Zs.A 6251: Show issues Location:
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  10. Article ; Online: A bacterial sulfoglycosidase highlights mucin O-glycan breakdown in the gut ecosystem

    Katoh, Toshihiko / Yamada, Chihaya / Wallace, Michael D. / Yoshida, Ayako / Gotoh, Aina / Arai, Moe / Maeshibu, Takako / Kashima, Toma / Hagenbeek, Arno / Ojima, Miriam N. / Takada, Hiromi / Sakanaka, Mikiyasu / Shimizu, Hidenori / Nishiyama, Keita / Ashida, Hisashi / Hirose, Junko / Suarez-Diez, Maria / Nishiyama, Makoto / Kimura, Ikuo /
    Stubbs, Keith A. / Fushinobu, Shinya / Katayama, Takane

    Nature Chemical Biology

    2023  Volume 19

    Abstract: Mucinolytic bacteria modulate host–microbiota symbiosis and dysbiosis through their ability to degrade mucin O-glycans. However, how and to what extent bacterial enzymes are involved in the breakdown process remains poorly understood. Here we focus on a ... ...

    Abstract Mucinolytic bacteria modulate host–microbiota symbiosis and dysbiosis through their ability to degrade mucin O-glycans. However, how and to what extent bacterial enzymes are involved in the breakdown process remains poorly understood. Here we focus on a glycoside hydrolase family 20 sulfoglycosidase (BbhII) from Bifidobacterium bifidum, which releases N-acetylglucosamine-6-sulfate from sulfated mucins. Glycomic analysis showed that, in addition to sulfatases, sulfoglycosidases are involved in mucin O-glycan breakdown in vivo and that the released N-acetylglucosamine-6-sulfate potentially affects gut microbial metabolism, both of which were also supported by a metagenomic data mining analysis. Enzymatic and structural analysis of BbhII reveals the architecture underlying its specificity and the presence of a GlcNAc-6S-specific carbohydrate-binding module (CBM) 32 with a distinct sugar recognition mode that B. bifidum takes advantage of to degrade mucin O-glycans. Comparative analysis of the genomes of prominent mucinolytic bacteria also highlights a CBM-dependent O-glycan breakdown strategy used by B. bifidum. [Figure not available: see fulltext.].
    Keywords Life Science
    Language English
    Publishing country nl
    Document type Article ; Online
    ZDB-ID 2202962-X
    ISSN 1552-4469 ; 1552-4450
    ISSN (online) 1552-4469
    ISSN 1552-4450
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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