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Article: Variability in Cross-Sectional Muscle Atrophy: Insights From the Central Rectus Femoris.

Takahashi, Yusuke / Okura, Kazuki

2023 Volume 15, Issue 11, Page(s) e49097

Abstract: Background and purpose: This study aimed to evaluate the non-uniformity of cross-sectional areas in atrophic muscles using the central aponeurosis (CA) as a marker for the central region of the rectus femoris (RF).: Methods: We enrolled 51 ... ...

Abstract	Background and purpose: This study aimed to evaluate the non-uniformity of cross-sectional areas in atrophic muscles using the central aponeurosis (CA) as a marker for the central region of the rectus femoris (RF). Methods: We enrolled 51 consecutively admitted patients (102 limbs) with aortic valve stenosis who were scheduled for elective surgical or catheter-based aortic valve replacement and were expected to have low physical activity-induced muscle atrophy. We obtained short-axis images of the mid-thigh using an ultrasonic diagnostic imaging system (with a 5-cm-wide probe) and measured the deviation of the central region of the rectus femoris from the body surface. Muscle thickness was measured using conventional morphological measurements on the body surface ("On Surface") and landmarks within the ultrasonographic image ("In Images"). Results: Displacements ≥ 1.5 cm were observed in 56 (54.9%) limbs, and displacements ≥ 2.5 cm were observed in 34 (33%) limbs. The displacements were predominantly in the medial direction and ranged from 4 cm to 1.5 cm. Among the cases in which the deviation was ≥2.5 cm, "On Surface" measurements resulted in images in which the vastus lateralis overlapped with the rectus femoris. The thickness of the rectus femoris was significantly lower with "On Surface" measurements than with "In Images" measurements (right, p < 0.001; left, p = 0.007), with a maximum difference of 10.5 mm. Conclusions: In conclusion, it was observed that the rectus femoris at the center was often displaced medially, and the muscle thickness was thinner at the edge than at the center, showing a non-uniform morphology.
Language	English
Publishing date	2023-11-20
Publishing country	United States
Document type	Journal Article
ZDB-ID	2747273-5
ISSN	2168-8184
ISSN	2168-8184
DOI	10.7759/cureus.49097
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Article: Novel wireless laser doppler flowmeter-based investigation of earlobe vascular dynamics in cardiopulmonary exercise testing.

Takahashi, Yusuke / Okura, Kazuki / Suto, Akiyoshi

Journal of physical therapy science

2024 Volume 36, Issue 4, Page(s) 202–207

Abstract: Purpose] A new wireless laser Doppler blood flowmeter has facilitated easier, more stable measurement of skin perfusion during exercise. However, earlobe blood flow during the cardiopulmonary exercise test remains unascertained. This study aimed to ... ...

Abstract	[Purpose] A new wireless laser Doppler blood flowmeter has facilitated easier, more stable measurement of skin perfusion during exercise. However, earlobe blood flow during the cardiopulmonary exercise test remains unascertained. This study aimed to clarify the characteristics of earlobe blood flow during incremental exercise load in healthy individuals. [Participants and Methods] Among 25 healthy males (age 23.6 ± 2.5 years), cycle ergometer-based symptom-limited cardiopulmonary exercise test, after 4 minutes of rest, was conducted with a 4-minute 20W warm-up and a continuous 2W-increase in the work rate every 6 seconds; earlobe blood flow was measured using a wireless laser Doppler blood flowmeter. [Results] Compared with that at rest, earlobe blood flow increased significantly from 50% of exercise peak intensity to a maximum of 1.7 times, but decreased immediately after exercise. The earlobe blood flow %change did not significantly correlate with hemodynamic parameters and its inflection point 36.4% Load
Language	English
Publishing date	2024-04-01
Publishing country	Japan
Document type	Journal Article
ZDB-ID	2038898-6
ISSN	0915-5287
ISSN	0915-5287
DOI	10.1589/jpts.36.202
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Article ; Online: [Preparation of Nanoplastic Particles as Potential Standards for the Study of Nanoplastics].

Tanaka, Kosuke / Takahashi, Yusuke / Kuramochi, Hidetoshi / Osako, Masahiro / Suzuki, Go

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan

2024 Volume 144, Issue 2, Page(s) 165–170

Abstract: Nanoplastics (NPs) are plastic fragments that are small enough to be absorbed by organisms through ingestion or inhalation. Recent studies indicate that nanoplastics can be ubiquitous in the environment, and there are growing concerns regarding the ... ...

Abstract	Nanoplastics (NPs) are plastic fragments that are small enough to be absorbed by organisms through ingestion or inhalation. Recent studies indicate that nanoplastics can be ubiquitous in the environment, and there are growing concerns regarding the impacts of nanoplastics on the health of humans and other organisms. However, quantitative information on nanoplastics in the environment is still very limited, and most previous toxicity studies have used only polystyrene (PS) particles because of a lack of appropriate model particles of other plastics. We developed a nanoprecipitation-based method for the preparation of nanoplastic particles of five major polymers: low-density polyethylene (LDPE), high-density polyethylene (HDPE), polypropylene (PP), polyvinyl chloride (PVC), and polystyrene. A major advantage of our method is that the nanoplastic particles are prepared without using reagents that can remain in the particles as impurities. Analysis of the prepared particles' molecular weight (M
MeSH term(s)	Humans ; Polystyrenes ; Plastics ; Microplastics/analysis ; Polyethylene ; Polymers
Chemical Substances	Polystyrenes ; Plastics ; Microplastics ; Polyethylene (9002-88-4) ; Polymers
Language	Japanese
Publishing date	2024-01-11
Publishing country	Japan
Document type	English Abstract ; Journal Article
ZDB-ID	200514-1
ISSN	1347-5231 ; 0031-6903 ; 0372-7750 ; 0919-2085 ; 0919-2131
ISSN (online)	1347-5231
ISSN	0031-6903 ; 0372-7750 ; 0919-2085 ; 0919-2131
DOI	10.1248/yakushi.23-00152-1
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Zs.A 643: Show issues

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Article ; Online: Trapped lung and refractory pleural effusion in a patient receiving peritoneal dialysis.

Sato, Yuya / Takahashi, Yusuke / Tasaki, Kazuyuki

Therapeutic apheresis and dialysis : official peer-reviewed journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy

2023 Volume 27, Issue 5, Page(s) 976–977

MeSH term(s)	Humans ; Pleural Effusion/etiology ; Pleural Effusion/therapy ; Peritoneal Dialysis ; Lung
Language	English
Publishing date	2023-04-21
Publishing country	Australia
Document type	Letter
ZDB-ID	2119809-3
ISSN	1744-9987 ; 1091-6660 ; 1744-9979
ISSN (online)	1744-9987
ISSN	1091-6660 ; 1744-9979
DOI	10.1111/1744-9987.13998
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Zs.A 5208: Show issues

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Article ; Online: Laparoscopic Removal of an Infected Mesh Following TEP Procedure for Femoral Hernia with Omental Patch Closure of a Peritoneal Defect.

Takahashi, Yusuke / Komatsu, Daisuke / Seki, Hitoshi

The American journal of case reports

2023 Volume 24, Page(s) e940618

Abstract: BACKGROUND Mesh infection following inguinal hernia repair is rare, and mesh removal is mandatory. However, the laparoscopic approach is challenging to perform. Here, we present a case of laparoscopic repair of a mesh infection using a totally ... ...

Abstract	BACKGROUND Mesh infection following inguinal hernia repair is rare, and mesh removal is mandatory. However, the laparoscopic approach is challenging to perform. Here, we present a case of laparoscopic repair of a mesh infection using a totally extraperitoneal approach (TEP). CASE REPORT A 76-year-old woman underwent repair of a right femoral hernia via TEP approach using a prosthetic mesh with unabsorbable tacks. A month and a half after the surgery, she reported pain in the right groin. Computed tomography revealed a subcutaneous abscess in the right groin. We suspected mesh infection and initially chose conservative management, which included percutaneous drainage and systemic antibiotic administration. Her symptoms temporarily resolved; however, symptom relapse and purulent discharge from the right groin were observed. We performed laparoscopic removal of the infected mesh and all tacks via the transabdominal preperitoneal approach. A drain was placed in the infected preperitoneal space, and the peritoneal defect was covered using the greater omentum. The patient's postoperative course was uneventful, and she was discharged on postoperative day 20. Infection relapse, symptoms of femoral hernia, and adhesive intestinal obstruction have not been observed. CONCLUSIONS A laparoscopic approach for mesh infection after TEP hernia repair is feasible, even if the mesh is fixed using a tack. Greater omental use for peritoneal defects is useful in clinical situations associated with a contaminated surgical field.
MeSH term(s)	Female ; Humans ; Aged ; Omentum/surgery ; Hernia, Femoral/surgery ; Surgical Mesh/adverse effects ; Peritoneum ; Laparoscopy/adverse effects
Language	English
Publishing date	2023-08-17
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	2517183-5
ISSN	1941-5923 ; 1941-5923
ISSN (online)	1941-5923
ISSN	1941-5923
DOI	10.12659/AJCR.940618
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Article ; Online: Properties of REM sleep alterations with epilepsy.

Ikoma, Yoko / Takahashi, Yusuke / Sasaki, Daichi / Matsui, Ko

Brain : a journal of neurology

2023 Volume 146, Issue 6, Page(s) 2431–2442

Abstract: It is usually assumed that individuals rest during sleep. However, coordinated neural activity that presumably requires high energy consumption is increased during REM sleep. Here, using freely moving male transgenic mice, the local brain environment and ...

Abstract	It is usually assumed that individuals rest during sleep. However, coordinated neural activity that presumably requires high energy consumption is increased during REM sleep. Here, using freely moving male transgenic mice, the local brain environment and astrocyte activity during REM sleep were examined using the fibre photometry method with an optical fibre inserted deep into the lateral hypothalamus, a region that is linked with controlling sleep and metabolic state of the entire brain. Optical fluctuations of endogenous autofluorescence of the brain parenchyma or fluorescence of sensors for Ca2+ or pH expressed in astrocytes were examined. Using a newly devised method for analysis, changes in cytosolic Ca2+ and pH in astrocytes and changes in the local brain blood volume (BBV) were extracted. On REM sleep, astrocytic Ca2+ decreases, pH decreases (acidification) and BBV increases. Acidification was unexpected, as an increase in BBV would result in efficient carbon dioxide and/or lactate removal, which leads to alkalinization of the local brain environment. Acidification could be a result of increased glutamate transporter activity due to enhanced neuronal activity and/or aerobic metabolism in astrocytes. Notably, optical signal changes preceded the onset of the electrophysiological property signature of REM sleep by ∼20-30 s. This suggests that changes in the local brain environment have strong control over the state of neuronal cell activity. With repeated stimulation of the hippocampus, seizure response gradually develops through kindling. After a fully kindled state was obtained with multiple days of stimuli, the optical properties of REM sleep at the lateral hypothalamus were examined again. Although a negative deflection of the detected optical signal was observed during REM sleep after kindling, the estimated component changed. The decrease in Ca2+ and increase in BBV were minimal, and a large decrease in pH (acidification) emerged. This acidic shift may trigger an additional gliotransmitter release from astrocytes, which could lead to a state of hyperexcitable brain. As the properties of REM sleep change with the development of epilepsy, REM sleep analysis may serve as a biomarker of epileptogenesis severity. REM sleep analysis may also predict whether a specific REM sleep episode triggers post-sleep seizures.
MeSH term(s)	Animals ; Mice ; Male ; Sleep, REM/physiology ; Calcium ; Epilepsy ; Sleep/physiology ; Seizures
Chemical Substances	Calcium (SY7Q814VUP)
Language	English
Publishing date	2023-03-02
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80072-7
ISSN	1460-2156 ; 0006-8950
ISSN (online)	1460-2156
ISSN	0006-8950
DOI	10.1093/brain/awac499
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Article: Antihypertensive Effects of Three Days of Leg Bathing on a Patient With Stanford Type A Acute Aortic Dissection After Surgery: A Case Report.

Takahashi, Yusuke / Hasegawa, Kakeru / Okura, Kazuki

Cureus

2023 Volume 15, Issue 8, Page(s) e43596

Abstract: Thermal therapy is expected to have an antihypertensive effect associated with increased blood flow and vasodilation. Here, we report a case of postoperative aortic dissection in which leg bathing was effective for treating hypertension. A 50-year-old ... ...

Abstract	Thermal therapy is expected to have an antihypertensive effect associated with increased blood flow and vasodilation. Here, we report a case of postoperative aortic dissection in which leg bathing was effective for treating hypertension. A 50-year-old female (body mass index: 25.3 kg/m
Language	English
Publishing date	2023-08-16
Publishing country	United States
Document type	Case Reports
ZDB-ID	2747273-5
ISSN	2168-8184
ISSN	2168-8184
DOI	10.7759/cureus.43596
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Article ; Online: Regioselective C-H Azidation of Anilines and Application to Synthesis of Key Intermediate for Pharmaceutical.

Seki, Masahiko / Takahashi, Yusuke

The Journal of organic chemistry

2021 Volume 86, Issue 11, Page(s) 7842–7848

Abstract: A catalytic system for regioselective C-H azidation of inactive anilines was developed. In the presence of ... ...

Abstract	A catalytic system for regioselective C-H azidation of inactive anilines was developed. In the presence of CuSO
MeSH term(s)	Aniline Compounds ; Antihypertensive Agents ; Catalysis ; Cyclization ; Pharmaceutical Preparations
Chemical Substances	Aniline Compounds ; Antihypertensive Agents ; Pharmaceutical Preparations
Language	English
Publishing date	2021-05-26
Publishing country	United States
Document type	Journal Article
ZDB-ID	123490-0
ISSN	1520-6904 ; 0022-3263
ISSN (online)	1520-6904
ISSN	0022-3263
DOI	10.1021/acs.joc.1c00734
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Zs.A 4473: Show issues

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Article ; Online: H3.1/3.2 regulate the initial progression of the gene expression program.

Funaya, Satoshi / Takahashi, Yusuke / Suzuki, Masataka G / Suzuki, Yutaka / Aoki, Fugaku

Nucleic acids research

2024

Abstract: In mice, transcription from the zygotic genome is initiated at the mid-one-cell stage, and occurs promiscuously in many areas of the genome, including intergenic regions. Regulated transcription from selected genes is established during the two-cell ... ...

Abstract	In mice, transcription from the zygotic genome is initiated at the mid-one-cell stage, and occurs promiscuously in many areas of the genome, including intergenic regions. Regulated transcription from selected genes is established during the two-cell stage. This dramatic change in the gene expression pattern marks the initiation of the gene expression program and is essential for early development. We investigated the involvement of the histone variants H3.1/3.2 in the regulation of changes in gene expression pattern during the two-cell stage. Immunocytochemistry analysis showed low nuclear deposition of H3.1/3.2 in the one-cell stage, followed by a rapid increase in the late two-cell stage. Where chromatin structure is normally closed between the one- and two-cell stages, it remained open until the late two-cell stage when H3.1/3.2 were knocked down by small interfering RNA. Hi-C analysis showed that the formation of the topologically associating domain was disrupted in H3.1/3.2 knockdown (KD) embryos. Promiscuous transcription was also maintained in the late two-cell stage in H3.1/3.2 KD embryos. These results demonstrate that H3.1/3.2 are involved in the initial process of the gene expression program after fertilization, through the formation of a closed chromatin structure to execute regulated gene expression during the two-cell stage.
Language	English
Publishing date	2024-04-03
Publishing country	England
Document type	Journal Article
ZDB-ID	186809-3
ISSN	1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048
ISSN (online)	1362-4962 ; 1362-4954
ISSN	0301-5610 ; 0305-1048
DOI	10.1093/nar/gkae214
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Zs.A 1067: Show issues

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Article ; Online: Anxiety control by astrocytes in the lateral habenula.

Tan, Wanqin / Ikoma, Yoko / Takahashi, Yusuke / Konno, Ayumu / Hirai, Hirokazu / Hirase, Hajime / Matsui, Ko

Neuroscience research

2024

Abstract: The potential role of astrocytes in lateral habenula (LHb) in modulating anxiety was explored in this study. The habenula are a pair of small nuclei located above the thalamus, known for their involvement in punishment avoidance and anxiety. Herein, we ... ...

Abstract	The potential role of astrocytes in lateral habenula (LHb) in modulating anxiety was explored in this study. The habenula are a pair of small nuclei located above the thalamus, known for their involvement in punishment avoidance and anxiety. Herein, we observed an increase in theta-band oscillations of local field potentials (LFPs) in the LHb when mice were exposed to anxiety-inducing environments. Electrical stimulation of LHb at theta-band frequency promoted anxiety-like behavior. Calcium (Ca
Language	English
Publishing date	2024-02-02
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	605842-5
ISSN	1872-8111 ; 0168-0102 ; 0921-8696
ISSN (online)	1872-8111
ISSN	0168-0102 ; 0921-8696
DOI	10.1016/j.neures.2024.01.006
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Zs.A 1993: Show issues

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