LIVIVO - Search results -

Search results

Result 1 - 10 of total 95

Search options

Article ; Online: Time elapsed between ovulation and insemination determines the quality of fertilized rat oocytes.

Nakagata, Naomi / Nakao, Satohiro / Mikoda, Nobuyuki / Yamaga, Katsuma / Takeo, Toru

The Journal of reproduction and development

2024 Volume 70, Issue 2, Page(s) 123–130

Abstract: Genetically modified rats are valuable models in human disease research. We recently developed an improved system for rat sperm cryopreservation and in vitro fertilization (IVF) that facilitates the efficient production and preservation of genetically ... ...

Abstract	Genetically modified rats are valuable models in human disease research. We recently developed an improved system for rat sperm cryopreservation and in vitro fertilization (IVF) that facilitates the efficient production and preservation of genetically modified rats. In the IVF procedure performed using frozen-thawed rat sperm, the IVF schedule is fixed to ensure timely hormone administration and oocyte collection. To enhance the flexibility of the IVF schedule, possible periods of postovulated rat oocytes with normal fertility and developmental abilities should be determined. Therefore, in this study, we examined the fertilization and developmental ability of incubated oocytes 1-13 h after oocyte collection at 9:00 AM. The fertilization rate decreased 7 h after oocyte collection, and abnormally fertilized oocytes appeared 10 h after oocyte collection. The developmental rate also decreased 7 h after oocyte collection; however, live pups were obtained from oocytes 12 h after oocyte collection. In summary, ovulated rat oocytes exhibited a high developmental ability after IVF for up to 4 h after oocyte collection.
MeSH term(s)	Female ; Male ; Rats ; Humans ; Animals ; Semen ; Fertilization in Vitro/methods ; Oocytes ; Cryopreservation/methods ; Ovulation ; Insemination
Language	English
Publishing date	2024-02-26
Publishing country	Japan
Document type	Journal Article
ZDB-ID	2039060-9
ISSN	1348-4400 ; 0916-8818
ISSN (online)	1348-4400
ISSN	0916-8818
DOI	10.1262/jrd.2023-067
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: High-concentration bovine serum albumin enhances fertilization ability of cold-stored rat sperm.

Yamaga, Katsuma / Nakao, Satohiro / Mikoda, Nobuyuki / Sztein, Jorge Mario / Nakagata, Naomi / Takeo, Toru

The Journal of reproduction and development

2024 Volume 70, Issue 2, Page(s) 131–137

Abstract: Cold transport of the cauda epididymides is a useful technique for shipping laboratory rat sperm. Cold transport of rat sperm avoids potential risks of microbiological infection, animal escape or death, and animal welfare issues. Previously, we reported ... ...

Abstract	Cold transport of the cauda epididymides is a useful technique for shipping laboratory rat sperm. Cold transport of rat sperm avoids potential risks of microbiological infection, animal escape or death, and animal welfare issues. Previously, we reported that a cold-storage solution containing dimethyl sulfoxide and quercetin maintained the fertility of cold-stored rat sperm. However, cold-stored rat sperm exhibited a decreased fertilization rate after 24-h storage. To recover the fertility of cold-stored sperm, we focused on the effects of bovine serum albumin (BSA), a cholesterol acceptor that induces sperm capacitation. We sought to determine the optimal concentration of BSA in fertilization medium based on the fertility of cold-stored rat sperm. High concentrations of BSA (40 mg/ml) enhanced the fertilization rate of cold-stored rat sperm and maintained sperm fertility for 144 h. Embryos derived from cold-stored and BSA-treated sperm normally developed into pups after embryo transfer. In summary, high BSA concentrations enhanced the fertility of cold-stored rat sperm and prolonged the storage period to 144 h, thereby expanding the transportable region for genetically engineered rats.
MeSH term(s)	Animals ; Rats ; Male ; Serum Albumin, Bovine/pharmacology ; Fertilization in Vitro/methods ; Semen ; Spermatozoa ; Sperm Capacitation
Chemical Substances	Serum Albumin, Bovine (27432CM55Q)
Language	English
Publishing date	2024-03-01
Publishing country	Japan
Document type	Journal Article
ZDB-ID	2039060-9
ISSN	1348-4400 ; 0916-8818
ISSN (online)	1348-4400
ISSN	0916-8818
DOI	10.1262/jrd.2023-085
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Observation of the in vitro fertilization process in living oocytes using frozen-thawed sperm in rats.

Nakagata, Naomi / Nakao, Satohiro / Mikoda, Nobuyuki / Yamaga, Katsuma / Takeo, Toru

Theriogenology

2023 Volume 199, Page(s) 69–76

Abstract: Previous studies have observed the fertilization process in rats using whole-mount preparation at different time-points after insemination. However, very few reports have described the various events during the fertilization process using an inverted ... ...

Abstract	Previous studies have observed the fertilization process in rats using whole-mount preparation at different time-points after insemination. However, very few reports have described the various events during the fertilization process using an inverted microscope without whole-mount. Moreover, to the best of our knowledge, no reports have described the observation of changes in sperm motility associated with sperm penetration into oocytes. In this study, in vitro fertilization was performed using frozen-thawed sperm in various rat strains (SD, Wistar, LE, F344, and BN) and oocytes from the SD strain, and the process of sperm penetration into the oocytes and the subsequent development were observed. The sperm motility was assessed, and the correlation between the process of sperm penetration into the oocytes and sperm motility over time was examined. The motility of frozen sperm from the SD, Wistar, LE, and F344 increased at 2-3 h after thawing, at which time the sperm attached themselves to the zona pellucida. Sperm penetration into the zona pellucida occurred after 3-5 h, and pronuclei were formed in the cytoplasm of oocytes 5-9 h after insemination. The fertilities of frozen-thawed sperm from the SD, Wistar, LE, and F344 were 92.7%, 90.0%, 90.7%, and 68.7%, respectively. However, no increase in motility was observed after thawing of frozen sperm from the BN, and the fertility was only 21%. In addition, very few polyspermic oocytes were observed with use of frozen-thawed sperm of all strains. In summary, rats are suitable animals for the observation of sperm penetration into the oocytes, and we determined the timing of fertilization events in IVF using frozen-thawed rat sperm.
MeSH term(s)	Male ; Rats ; Animals ; Rats, Inbred F344 ; Rats, Wistar ; Sperm Motility ; Semen ; Fertilization in Vitro/veterinary ; Oocytes ; Spermatozoa ; Sperm-Ovum Interactions ; Cryopreservation/veterinary
Language	English
Publishing date	2023-01-18
Publishing country	United States
Document type	Journal Article
ZDB-ID	189232-0
ISSN	1879-3231 ; 0093-691X
ISSN (online)	1879-3231
ISSN	0093-691X
DOI	10.1016/j.theriogenology.2023.01.016
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 3929: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Cryobanking and Recovery of Genetically Modified Mice.

Takeo, Toru / Nakagata, Naomi

Methods in molecular biology (Clifton, N.J.)

2019 Volume 2066, Page(s) 195–209

Abstract: Cryobanking of sperm, oocytes, and embryos is a useful means to efficiently maintain mouse colonies without breeding live animals. Cryopreserved cells can be permanently stored in well-managed systems in liquid nitrogen tanks at -196 °C and quickly ... ...

Abstract	Cryobanking of sperm, oocytes, and embryos is a useful means to efficiently maintain mouse colonies without breeding live animals. Cryopreserved cells can be permanently stored in well-managed systems in liquid nitrogen tanks at -196 °C and quickly reanimated for use via in vitro fertilization and/or embryo transfer. Recent improvements of reproductive technology markedly enhanced the efficiency of recovering and producing animals using cryopreserved cells. The establishment of a cryobanking system will increase the performance of animal experiments, meet the principles of 3Rs (replacement, reduction, and refinement), and reduce labour and costs. In this chapter, we described the latest techniques of sperm cryopreservation, in vitro fertilization, and oocyte and two-cell embryo vitrification developed at the Center for Animal Resources and Development (CARD).
MeSH term(s)	Animals ; Cryopreservation/methods ; Embryo Transfer/methods ; Female ; Fertilization in Vitro/methods ; Humans ; Male ; Mice ; Mice, Transgenic/genetics ; Oocytes/growth & development ; Organisms, Genetically Modified/genetics ; Pregnancy ; Pregnancy Rate ; Spermatozoa/growth & development ; Vitrification
Language	English
Publishing date	2019-11-13
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-4939-9837-1_16
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- Full text online
- Order with fees

Article ; Online: Basic mouse reproductive techniques developed and modified at the Center for Animal Resources and Development (CARD), Kumamoto University.

Nakagata, Naomi / Takeo, Toru

Experimental animals

2019 Volume 68, Issue 4, Page(s) 391–395

Abstract: The Center for Animal Resources and Development (CARD), Kumamoto University was established in 1998. We provide advanced research support services for the mouse-based biomedical research community via an official and a premium mouse bank system. To ... ...

Abstract	The Center for Animal Resources and Development (CARD), Kumamoto University was established in 1998. We provide advanced research support services for the mouse-based biomedical research community via an official and a premium mouse bank system. To efficiently manage these mouse banks, we have actively developed and modified basic mouse reproductive techniques. We shall introduce these techniques in this paper.
MeSH term(s)	Animals ; Animals, Genetically Modified/physiology ; Animals, Laboratory/physiology ; Biomedical Research ; Disease Models, Animal ; Japan ; Laboratory Animal Science ; Mice/physiology ; Reproduction ; Reproductive Techniques
Language	English
Publishing date	2019-06-25
Publishing country	Japan
Document type	Journal Article ; Review
ZDB-ID	2088228-2
ISSN	1881-7122 ; 1341-1357 ; 0007-5124
ISSN (online)	1881-7122
ISSN	1341-1357 ; 0007-5124
DOI	10.1538/expanim.19-0070
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 1653: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Synchronization of the ovulation and copulation timings increased the number of in vivo fertilized oocytes in superovulated female mice.

Nakao, Satohiro / Ito, Kotono / Sugahara, Chihiro / Watanabe, Hitomi / Kondoh, Gen / Nakagata, Naomi / Takeo, Toru

PloS one

2023 Volume 18, Issue 2, Page(s) e0281330

Abstract: The number of sperm that reaches the oocytes in mammalian species is limited. In mice, 8-10 oocytes are ovulated, a similar number of sperm reaches the oocytes, and nearly all oocytes are fertilized via natural mating. Meanwhile, our improved ... ...

Abstract	The number of sperm that reaches the oocytes in mammalian species is limited. In mice, 8-10 oocytes are ovulated, a similar number of sperm reaches the oocytes, and nearly all oocytes are fertilized via natural mating. Meanwhile, our improved superovulation technique (ultrasuperovulation: administration of inhibin antiserum and equine chorionic gonadotropin [IASe]) produced 100 oocytes from a single female C57BL/6 mouse but resulted in only approximately 20 fertilized oocytes via mating. We hypothesized that sperm shortage in the ampulla might cause this low fertilization rate. Mice were mated in the proestrus stage or after hormone injection, but ovulation timing was not considered. In clinical application, the rhythm method supports fertilization by testing the ovulation period and synchronizing the ovulation and copulation timings. Therefore, this study examined the effects of ovulation and copulation timings on in vivo fertilization in female mice with IASe. Synchronization of the ovulation and copulation timings increased fertilization efficiency in female mice with ultrasuperovulation. The number of embryos obtained post ovulation was three times higher than that obtained pre ovulation. This study suggests that synchronized ovulation and copulation timings improve the efficiency of in vivo fertilization in IASe-treated female mice. This technique can be used to produce genetically modified mice and develop technologies for infertility treatment.
MeSH term(s)	Mice ; Male ; Female ; Animals ; Horses ; Copulation ; Mice, Inbred C57BL ; Semen ; Ovulation ; Superovulation ; Oocytes ; Mammals
Language	English
Publishing date	2023-02-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0281330
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Mouse Sperm Cryopreservation Using Cryoprotectant Containing l-Glutamine.

Takeo, Toru / Nakagata, Naomi

Cold Spring Harbor protocols

2018 Volume 2018, Issue 6

Abstract: Efforts to advance sperm cryopreservation are ongoing and include modifications in the cryoprotective agents. The addition of l-glutamine maintains post-thaw motility and reduces plasma membrane damage to sperm. ...

Abstract	Efforts to advance sperm cryopreservation are ongoing and include modifications in the cryoprotective agents. The addition of l-glutamine maintains post-thaw motility and reduces plasma membrane damage to sperm.
MeSH term(s)	Animals ; Cryopreservation ; Cryoprotective Agents/pharmacology ; Freezing ; Glutamine/pharmacology ; Humans ; Male ; Mice ; Semen Preservation ; Spermatozoa/drug effects ; Spermatozoa/physiology
Chemical Substances	Cryoprotective Agents ; Glutamine (0RH81L854J)
Language	English
Publishing date	2018-06-01
Publishing country	United States
Document type	Journal Article
ISSN	1559-6095
ISSN (online)	1559-6095
DOI	10.1101/pdb.prot094516
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: In Vitro Fertilization in Mice.

Takeo, Toru / Nakagata, Naomi

Cold Spring Harbor protocols

2018 Volume 2018, Issue 6

Abstract: The success of in vitro fertilization is strongly influenced by genetic background, handling of sperm and oocytes, and culture conditions. This protocol promotes enhanced rates of fertilization by using preincubation medium for sperm containing methyl-β- ... ...

Abstract	The success of in vitro fertilization is strongly influenced by genetic background, handling of sperm and oocytes, and culture conditions. This protocol promotes enhanced rates of fertilization by using preincubation medium for sperm containing methyl-β-cyclodextrin (MBCD) and fertilization medium with high calcium and reduced glutathione (GSH).
MeSH term(s)	Animals ; Cells, Cultured ; Cryopreservation ; Embryo Culture Techniques ; Embryo Transfer ; Female ; Fertilization ; Fertilization in Vitro/methods ; Humans ; Insemination ; Male ; Mice ; Oocytes/cytology ; Specimen Handling ; Spermatozoa/cytology
Language	English
Publishing date	2018-06-01
Publishing country	United States
Document type	Journal Article
ISSN	1559-6095
ISSN (online)	1559-6095
DOI	10.1101/pdb.prot094524
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Efficient production of immunodeficient non-obese diabetic/Shi-scid

Goto, Motohito / Takeo, Toru / Takahashi, Riichi / Nakagata, Naomi

Laboratory animals

2020 Volume 55, Issue 1, Page(s) 13–20

Abstract: Severe immunodeficient mice are an essential tool for the examination of the efficacy and safety of new therapeutic technologies as a humanized model. Previously, non-obese diabetic (NOD)/Shi- ... ...

Abstract	Severe immunodeficient mice are an essential tool for the examination of the efficacy and safety of new therapeutic technologies as a humanized model. Previously, non-obese diabetic (NOD)/Shi-scid
MeSH term(s)	Animals ; Breeding/methods ; Female ; Gonadotropins, Equine/chemistry ; Immune Sera/chemistry ; Inhibins/chemistry ; Male ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Superovulation
Chemical Substances	Gonadotropins, Equine ; Immune Sera ; Inhibins (57285-09-3)
Language	English
Publishing date	2020-06-06
Publishing country	England
Document type	Journal Article
ZDB-ID	391008-8
ISSN	1758-1117 ; 0023-6772
ISSN (online)	1758-1117
ISSN	0023-6772
DOI	10.1177/0023677220928091
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 1951: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Dimethyl-α-cyclodextrin induces capacitation by removing phospholipids from the plasma membrane of mouse sperm†.

Nakao, Satohiro / Ito, Kotono / Sakoh, Kazuhito / Takemoto, Kenji / Watanabe, Hitomi / Kondoh, Gen / Irie, Tetsumi / Nakagata, Naomi / Takeo, Toru

Biology of reproduction

2023 Volume 108, Issue 4, Page(s) 671–681

Abstract: Capacitation is an important event in the completion of fertilization by mammalian sperm. Cholesterol efflux is a trigger of capacitation. In general, cholesterol acceptors of albumin and β-cyclodextrins are used to induce capacitation during in vitro ... ...

Abstract	Capacitation is an important event in the completion of fertilization by mammalian sperm. Cholesterol efflux is a trigger of capacitation. In general, cholesterol acceptors of albumin and β-cyclodextrins are used to induce capacitation during in vitro fertilization. Previously, we reported that methyl-β-cyclodextrin (MBCD), which is composed of seven glucoses, had a higher ability to induce capacitation than bovine serum albumin (BSA) in frozen-thawed mouse sperm. Comparison of albumin and cyclodextrins is helpful for understanding the mechanism of capacitation. In this study, we examined the effects of albumin, MBCD, and a different type of cyclodextrin, dimethyl-α-cyclodextrin (DMACD), which is composed of six glucoses, on several events of sperm capacitation. We showed that DMACD induced sperm capacitation and promoted fertilization ability. The time required to increase the fertilization rate differed among BSA, MBCD, and DMACD. BSA and MBCD enhanced cholesterol and phospholipid efflux, whereas DMACD enhanced only phospholipid efflux. BSA, MBCD, and DMACD increased sperm membrane fluidity, rearrangement of the lipid raft, and the acrosome reaction. These findings suggest that phospholipid efflux is a novel trigger of capacitation. Increasing the choice of sperm capacitation inducers may be useful for improving in vitro fertilization (IVF) techniques not only in mice, but also in various species in which it has been difficult to produce embryos by IVF.
MeSH term(s)	Male ; Animals ; Mice ; Phospholipids/metabolism ; Phospholipids/pharmacology ; Semen/metabolism ; Spermatozoa/metabolism ; Cholesterol/metabolism ; Sperm Capacitation ; Serum Albumin, Bovine/metabolism ; Serum Albumin, Bovine/pharmacology ; Cell Membrane/metabolism ; Mammals/metabolism
Chemical Substances	dimethyl-alpha-cyclodextrin ; Phospholipids ; Cholesterol (97C5T2UQ7J) ; Serum Albumin, Bovine (27432CM55Q)
Language	English
Publishing date	2023-02-12
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1118-6
ISSN	1529-7268 ; 0006-3363
ISSN (online)	1529-7268
ISSN	0006-3363
DOI	10.1093/biolre/ioad013
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 551: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito