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  1. Article ; Online: Porous Polymeric Nanofilms for Recreating the Basement Membrane in an Endothelial Barrier-on-Chip.

    Mancinelli, Elena / Zushi, Nanami / Takuma, Megumi / Cheng Chau, Chalmers Chi / Parpas, George / Fujie, Toshinori / Pensabene, Virginia

    ACS applied materials & interfaces

    2024  Volume 16, Issue 10, Page(s) 13006–13017

    Abstract: Organs-on-chips (OoCs) support an organotypic human cell ... ...

    Abstract Organs-on-chips (OoCs) support an organotypic human cell culture
    MeSH term(s) Humans ; Basement Membrane ; Porosity ; Polymers/chemistry ; Endothelium ; Human Umbilical Vein Endothelial Cells
    Chemical Substances Polymers
    Language English
    Publishing date 2024-02-27
    Publishing country United States
    Document type Journal Article
    ISSN 1944-8252
    ISSN (online) 1944-8252
    DOI 10.1021/acsami.3c16134
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Nanosheet Promotes Chronic Wound Healing by Localizing Uncultured Stromal Vascular Fraction Cells.

    Aoki, Shimpo / Endo, Yori / Guo, Cynthia / Wu, Mengfan / Kim, Audrey / Takuma, Megumi / Mroueh, Jessica / Weber, Luisa / Fujie, Toshinori / Nuutila, Kristo / Sinha, Indranil

    Advances in wound care

    2024  

    Abstract: Objective: ...

    Abstract Objective:
    Language English
    Publishing date 2024-04-16
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 2650541-1
    ISSN 2162-1934 ; 2162-1918
    ISSN (online) 2162-1934
    ISSN 2162-1918
    DOI 10.1089/wound.2024.0014
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Enhanced cellular engraftment of adipose-derived mesenchymal stem cell spheroids by using nanosheets as scaffolds.

    Nagano, Hisato / Suematsu, Yoshitaka / Takuma, Megumi / Aoki, Shimpo / Satoh, Ayano / Takayama, Eiji / Kinoshita, Manabu / Morimoto, Yuji / Takeoka, Shinji / Fujie, Toshinori / Kiyosawa, Tomoharu

    Scientific reports

    2021  Volume 11, Issue 1, Page(s) 14500

    Abstract: The short survival time of transplanted adipose-derived mesenchymal stem cells (ASCs) is a problem for skin wound healing. Transplantation after the formation of cellular spheroids has been investigated as a promising method for prolonging cellular ... ...

    Abstract The short survival time of transplanted adipose-derived mesenchymal stem cells (ASCs) is a problem for skin wound healing. Transplantation after the formation of cellular spheroids has been investigated as a promising method for prolonging cellular survival. However, there have been technical restrictions for transplantation of spheroids in clinical practice. Here, we show an effective method for transplantation of ASC spheroids onto skin wounds in order to efficiently cure refractory ulcers. To assist anchoring of spheroids onto skin wounds, we used a 120-nm-thick free-standing film (nanosheet) that has a highly adhesive property. Bioluminescence imaging showed that ASC spheroids carried by the nanosheet survived for 14 days, which is about two-times longer than that previously reported. Wounds treated with a nanosheet carrying ASC spheroids were 4-times smaller than untreated wounds on day 14. This method for transplantation of spheroids could be applied to cell therapy for various refractory skin wounds.
    MeSH term(s) Animals ; Cell Culture Techniques/instrumentation ; Cell Culture Techniques/methods ; Male ; Mesenchymal Stem Cell Transplantation/instrumentation ; Mesenchymal Stem Cell Transplantation/methods ; Mesenchymal Stem Cells/cytology ; Mice, Inbred C57BL ; Mitomycin/toxicity ; Nanostructures/chemistry ; Skin Ulcer/chemically induced ; Skin Ulcer/therapy ; Spheroids, Cellular ; Wound Healing ; Mice
    Chemical Substances Mitomycin (50SG953SK6)
    Language English
    Publishing date 2021-07-14
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-021-93642-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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