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Article ; Online: Reducing our carbon footprint in the haematology laboratory: A shared responsibility.

Fan, Bingwen Eugene / Tan, Jun Guan / Favaloro, Emmanuel J

International journal of laboratory hematology

2023 Volume 45, Issue 5, Page(s) 778–780

MeSH term(s)	Humans ; Carbon Footprint ; Hematology ; Laboratories, Clinical
Language	English
Publishing date	2023-03-26
Publishing country	England
Document type	Letter ; Comment
ZDB-ID	2268590-X
ISSN	1751-553X ; 1751-5521 ; 0141-9854
ISSN (online)	1751-553X
ISSN	1751-5521 ; 0141-9854
DOI	10.1111/ijlh.14060
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Article ; Online: Authors' reply to 'Plasma clearance and lipaemic index of lipid emulsion used for lipid emulsion treatment'.

Tan, Jun Guan / Wong, Moh Sim

Annals of clinical biochemistry

2021 Volume 58, Issue 6, Page(s) 666–667

MeSH term(s)	Diagnostic Tests, Routine ; Emulsions ; Humans ; Kinetics ; Plasma ; Triglycerides
Chemical Substances	Emulsions ; Triglycerides
Language	English
Publishing date	2021-04-12
Publishing country	England
Document type	Letter ; Comment
ZDB-ID	390309-6
ISSN	1758-1001 ; 0004-5632
ISSN (online)	1758-1001
ISSN	0004-5632
DOI	10.1177/00045632211007158
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Article ; Online: Does the use of fish oil-based lipid emulsion in the clinical setting of total parenteral nutrition and lipid rescue therapy interfere with common laboratory analytes on Roche Cobas 6000?

Tan, Jun Guan / Wong, Moh Sim

Annals of clinical biochemistry

2021 Volume 58, Issue 3, Page(s) 220–229

Abstract: Background: Lipaemic interference on automated analysers has been widely studied using soy-based emulsion such as Intralipid. Due to the greater adoption of fish oil-based lipid emulsion for total parenteral nutrition in view of improved clinical ... ...

Abstract	Background: Lipaemic interference on automated analysers has been widely studied using soy-based emulsion such as Intralipid. Due to the greater adoption of fish oil-based lipid emulsion for total parenteral nutrition in view of improved clinical outcomes, we seek to characterize the optical properties of SMOFlipid 20% (Fresenius Kabi, Bad Homburg, Germany), a fish oil-based emulsion, on the Roche Cobas 6000 chemistry analyser (Roche Diagnostic, Basel, Switzerland). Method: Various amounts of SMOFlipid were spiked into pooled serums. We plotted Roche Cobas Serum Index Gen.2 Lipaemia Index (L-index) against the amount of SMOFlipid added. We then studied the interference thresholds for aspartate aminotransferase, alanine aminotransferase, albumin and renal panel analytes using SMOFlipid. We subjected five levels of spiked lipaemia to high-speed centrifugation and analysed the specimens pre- and post-centrifugation. To postulate whether fish oil-based lipid emulsion interferes with laboratory results in the clinical setting, we calculated concentrations of SMOFlipid post-lipid rescue therapy and steady-state concentration of a typical total parenteral nutrition regime using pharmacokinetic principles. Results: SMOFlipid optical behaviour is similar to Intralipid using the Serum Index Gen.2 L-index, with 1 mg/dL of SMOFlipid representing 1 unit of L-index. Manufacturer-stated interference thresholds are accurate for alanine aminotransferase, aspartate aminotransferase, albumin, urea and creatinine. High-speed centrifugation at 60 min 21,100 Conclusion: Based on the interference thresholds we verified and pharmacokinetics parameters provided by SMOFlipid manufacturer, total parenteral nutrition may not interfere with chemistry analytes given sufficient clearance, but lipid rescue therapy will interfere. Further studies assessing lipaemic interference on immunoassays are needed.
MeSH term(s)	Alanine Transaminase/blood ; Aspartate Aminotransferases/blood ; Clinical Laboratory Techniques/methods ; Fat Emulsions, Intravenous/adverse effects ; Fat Emulsions, Intravenous/therapeutic use ; Fish Oils/adverse effects ; Fish Oils/therapeutic use ; Humans ; Laboratories ; Liver/metabolism ; Olive Oil/adverse effects ; Olive Oil/therapeutic use ; Parenteral Nutrition, Total/methods ; Serum Albumin/analysis ; Soybean Oil/adverse effects ; Soybean Oil/therapeutic use ; Triglycerides/adverse effects ; Triglycerides/analysis ; Triglycerides/therapeutic use
Chemical Substances	Fat Emulsions, Intravenous ; Fish Oils ; Olive Oil ; Serum Albumin ; Triglycerides ; Soybean Oil (8001-22-7) ; SMOFlipid (C9H2L21V7U) ; Aspartate Aminotransferases (EC 2.6.1.1) ; Alanine Transaminase (EC 2.6.1.2)
Language	English
Publishing date	2021-01-27
Publishing country	England
Document type	Journal Article
ZDB-ID	390309-6
ISSN	1758-1001 ; 0004-5632
ISSN (online)	1758-1001
ISSN	0004-5632
DOI	10.1177/0004563220986593
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Article ; Online: What are the appropriate reference limits for the diagnosis of hypophosphataemia in paediatric patients?

Tan, Jun Guan / Vasanwala, Rashida F / Yap, Fabian / Lek, Ngee / Ho, Clement K M

Journal of clinical pathology

2019 Volume 72, Issue 8, Page(s) 569–572

MeSH term(s)	Child ; Child, Preschool ; Female ; Humans ; Hypophosphatemia/blood ; Hypophosphatemia/diagnosis ; Infant ; Male ; Phosphates/blood ; Reference Values
Chemical Substances	Phosphates
Language	English
Publishing date	2019-04-17
Publishing country	England
Document type	Letter
ZDB-ID	80261-x
ISSN	1472-4146 ; 0021-9746
ISSN (online)	1472-4146
ISSN	0021-9746
DOI	10.1136/jclinpath-2019-205736
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Article: Insulin Allergy to Detemir Followed by Rapid Onset of Diabetic Ketoacidosis: A Case Report and Literature Review.

Teo, Chong Boon / Tan, Pek Yan / Lee, Shan Xian / Khoo, Joan / Tan, Jun Guan / Ang, Su Fen / Tan, Sze Hwa / Tay, Tunn Lin / Tan, Eberta / Lim, Su Chi / Boehm, Bernhard O / Loh, Wann Jia

Frontiers in endocrinology

2022 Volume 13, Page(s) 844040

Abstract: The management of diabetes mellitus in an insulin-dependent patient is challenging in the setting of concomitant antibody-mediated-insulin hypersensitivity. We report a case of a 62-year-old woman with pre-existing type 2 diabetes mellitus of 10 years ... ...

Abstract	The management of diabetes mellitus in an insulin-dependent patient is challenging in the setting of concomitant antibody-mediated-insulin hypersensitivity. We report a case of a 62-year-old woman with pre-existing type 2 diabetes mellitus of 10 years duration who developed type 3 hypersensitivity reaction to insulin analogue detemir, and subsequently, severe diabetic ketoacidosis (DKA). She was C-peptide negative and was diagnosed with insulin-dependent diabetes. Despite increasing dose adjustments, insulin-meal matching, and compliance with insulin, she experienced episodes of unexpected hyperglycaemia and hypoglycaemia. The development of rash after detemir initiation and rapid progression to DKA suggests an aberrant immune response leading to the insulin allergy and antibody-induced interference with insulin analogues. Glycaemic control in the patient initially improved after being started on subcutaneous insulin infusion pump with reduced insulin requirements. However, after a year on pump therapy, localised insulin hypersensitivity reactions started, and glycaemic control gradually deteriorated.
MeSH term(s)	Diabetes Mellitus, Type 2/complications ; Diabetes Mellitus, Type 2/drug therapy ; Diabetic Ketoacidosis/chemically induced ; Diabetic Ketoacidosis/drug therapy ; Female ; Humans ; Hypersensitivity/drug therapy ; Insulin/adverse effects ; Insulin Infusion Systems ; Middle Aged
Chemical Substances	Insulin
Language	English
Publishing date	2022-03-08
Publishing country	Switzerland
Document type	Case Reports ; Review
ZDB-ID	2592084-4
ISSN	1664-2392
ISSN	1664-2392
DOI	10.3389/fendo.2022.844040
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Article ; Online: Compound in-cis JAK2 mutant polycythemia vera manifesting as first-onset transient ischemic attack with erythrocytosis and an attenuated JAK2 V617F mutant signal on a quantitative DD-PCR assay.

Ng, Isaac K S / Lee, Peak-Ling / Ng, Li Jie / Chua, Rui Ping / Ng, Yu-Jin Alvin / Lee, Chun Kiat / Tan, Jun Guan / Chan, Tim Hon Man / Yan, Benedict / Prasad, Vishnu

International journal of laboratory hematology

2022 Volume 45, Issue 2, Page(s) e28–e31

MeSH term(s)	Humans ; Ischemic Attack, Transient/etiology ; Ischemic Attack, Transient/genetics ; Janus Kinase 2/genetics ; Mutation ; Polycythemia/diagnosis ; Polycythemia/etiology ; Polycythemia/genetics ; Polycythemia Vera/complications ; Polycythemia Vera/diagnosis ; Polycythemia Vera/genetics ; Polymerase Chain Reaction
Chemical Substances	JAK2 protein, human (EC 2.7.10.2) ; Janus Kinase 2 (EC 2.7.10.2)
Language	English
Publishing date	2022-10-07
Publishing country	England
Document type	Letter
ZDB-ID	2268590-X
ISSN	1751-553X ; 1751-5521 ; 0141-9854
ISSN (online)	1751-553X
ISSN	1751-5521 ; 0141-9854
DOI	10.1111/ijlh.13979
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Article: Radiation emergency preparedness: perception of non‑physician first receivers.

Lim, Hoon Chin Steven / Tan, Jun Guan / Hezan, Muhammad Azmi

Singapore medical journal

2014 Volume 55, Issue 7, Page(s) 392

MeSH term(s)	Bombs ; Cesium/poisoning ; Humans ; Polonium/poisoning ; Radiation Injuries ; Terrorism
Chemical Substances	Cesium (1KSV9V4Y4I) ; Polonium (DQY03U61EJ)
Language	English
Publishing date	2014-08-02
Publishing country	Singapore
Document type	Letter ; Comment
ZDB-ID	604319-7
ISSN	0037-5675
ISSN	0037-5675
DOI	10.11622/smedj.2014094
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Article ; Online: Commutability assessment of human urine certified reference materials for albumin and creatinine on multiple clinical analyzers using different statistical models

Liu, Hong / Ng, Cheng Yang / Liu, Qinde / Teo, Tang Lin / Loh, Tze Ping / Wong, Moh Sim / Sethi, Sunil Kumar / Tan, Jun Guan / Heng, Ping Ying / Saw, Sharon / Lam, Leslie Choong-Weng / Lee, Joanne Mee-Yin / Khaled, Khairee Bin / Phyu, Hnin Pwint / Ong, Noel Wan Ting

Anal Bioanal Chem. 2023 Feb., v. 415, no. 5 p.787-800

2023

Abstract: Urine albumin concentration and albumin–creatinine ratio are important for the screening of early-stage kidney damage. Commutable urine certified reference materials (CRMs) for albumin and creatinine are necessary for standardization of urine albumin and ...

Abstract	Urine albumin concentration and albumin–creatinine ratio are important for the screening of early-stage kidney damage. Commutable urine certified reference materials (CRMs) for albumin and creatinine are necessary for standardization of urine albumin and accurate measurement of albumin–urine ratio. Two urine CRMs for albumin and creatinine with certified values determined using higher-order reference measurement procedures were evaluated for their commutability on five brands/models of clinical analyzers where different reagent kits were used, including Roche Cobas c702, Roche Cobas c311, Siemens Atellica CH, Beckman Coulter AU5800, and Abbott Architect c16000. The commutability study was conducted by measuring at least 26 authentic patient urine samples and the human urine CRMs using both reference measurement procedures and the routine methods. Both the linear regression model suggested by the Clinical and Laboratory Standard Institute (CLSI) guidelines and log-transformed model recommended by the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) Commutability Working Group were used to evaluate the commutability of the human urine CRMs. The commutability of the human urine CRMs was found to be generally satisfactory on all five clinical analyzers for both albumin and creatinine, suggesting that they are suitable to be used routinely by clinical laboratories as quality control or for method validation of urine albumin and creatinine measurements.
Keywords	albumins ; chemistry ; creatinine ; humans ; kidneys ; medicine ; models ; patients ; quality control ; regression analysis ; urine
Language	English
Dates of publication	2023-02
Size	p. 787-800.
Publishing place	Springer Berlin Heidelberg
Document type	Article ; Online
ISSN	1618-2642
DOI	10.1007/s00216-022-04472-y
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Commutability assessment of human urine certified reference materials for albumin and creatinine on multiple clinical analyzers using different statistical models.

Analytical and bioanalytical chemistry

2022 Volume 415, Issue 5, Page(s) 787–800

Abstract: Urine albumin concentration and albumin-creatinine ratio are important for the screening of early-stage kidney damage. Commutable urine certified reference materials (CRMs) for albumin and creatinine are necessary for standardization of urine albumin and ...

Abstract	Urine albumin concentration and albumin-creatinine ratio are important for the screening of early-stage kidney damage. Commutable urine certified reference materials (CRMs) for albumin and creatinine are necessary for standardization of urine albumin and accurate measurement of albumin-urine ratio. Two urine CRMs for albumin and creatinine with certified values determined using higher-order reference measurement procedures were evaluated for their commutability on five brands/models of clinical analyzers where different reagent kits were used, including Roche Cobas c702, Roche Cobas c311, Siemens Atellica CH, Beckman Coulter AU5800, and Abbott Architect c16000. The commutability study was conducted by measuring at least 26 authentic patient urine samples and the human urine CRMs using both reference measurement procedures and the routine methods. Both the linear regression model suggested by the Clinical and Laboratory Standard Institute (CLSI) guidelines and log-transformed model recommended by the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) Commutability Working Group were used to evaluate the commutability of the human urine CRMs. The commutability of the human urine CRMs was found to be generally satisfactory on all five clinical analyzers for both albumin and creatinine, suggesting that they are suitable to be used routinely by clinical laboratories as quality control or for method validation of urine albumin and creatinine measurements.
MeSH term(s)	Humans ; Creatinine ; Reference Standards ; Quality Control ; Albumins ; Models, Statistical
Chemical Substances	Creatinine (AYI8EX34EU) ; Albumins
Language	English
Publishing date	2022-12-23
Publishing country	Germany
Document type	Journal Article
ZDB-ID	201093-8
ISSN	1618-2650 ; 0016-1152 ; 0372-7920
ISSN (online)	1618-2650
ISSN	0016-1152 ; 0372-7920
DOI	10.1007/s00216-022-04472-y
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Article ; Online: Inter-rater reliability of J-point location and measurement of the magnitude of ST segment elevation at the J-point on ECGs of STEMI patients by emergency department doctors.

Lim, Hoon Chin Steven / Salandanan, Edgar Azada / Phillips, Rachel / Tan, Jun Guan / Hezan, Muhammad Azmi

Emergency medicine journal : EMJ

2015 Volume 32, Issue 10, Page(s) 809–812

Abstract: Introduction and objectives: Identification of the J-point and measurement of ST segment elevation at the J-point are important for the diagnosis of ST-elevation myocardial infarction (STEMI). We conducted a study to determine the inter-rater ... ...

Abstract	Introduction and objectives: Identification of the J-point and measurement of ST segment elevation at the J-point are important for the diagnosis of ST-elevation myocardial infarction (STEMI). We conducted a study to determine the inter-rater reliability (IRR) of J-point location and measurement of the magnitude of ST elevation at the J-point on ECGs of patients with STEMI by emergency department (ED) doctors. Subjects and methods: Each participant examined 20 STEMI ECGs during a 1-month period in 2013. The participants were required to locate the J-point by selecting the small 1 mm square within which the J-point is located and measure the magnitude of ST elevation at the J-point identified (rounded up to the nearest 0.5 mm). The intraclass correlation coefficient (ICC) was calculated to assess the IRR. Results: Thirty doctors participated. The ICC assessing the degree to which all participants provided agreement in their assessment of the location of J-points across ECGs was 0.85 (95% CI 0.75 to 0.93), which is in the excellent range. The ICC for assessing the magnitude of ST elevation was 0.97 (95% CI 0.94 to 0.98), indicating excellent agreement as well. Conclusions: ED doctors show a high level of agreement when determining the location of J-points and measuring the magnitude of ST elevation at those J-points on ECGs of patients with STEMI. The findings support the measurement of ST segment elevation at the J-point in STEMI cases and should be regarded as a consistent standard to avoid confusion.
MeSH term(s)	Adult ; Brugada Syndrome ; Cardiac Conduction System Disease ; Electrocardiography/methods ; Emergency Service, Hospital/statistics & numerical data ; Female ; Humans ; Male ; Middle Aged ; Myocardial Infarction/diagnosis ; Observer Variation ; Reproducibility of Results ; Retrospective Studies
Language	English
Publishing date	2015-01-23
Publishing country	England
Document type	Journal Article
ZDB-ID	2040124-3
ISSN	1472-0213 ; 1472-0205
ISSN (online)	1472-0213
ISSN	1472-0205
DOI	10.1136/emermed-2014-204102
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