Article ; Online: Contact-Free Co-Culture Model for the Study of Innate Immune Cell Activation During Respiratory Virus Infection.
Journal of visualized experiments : JoVE
2021 , Issue 168
Abstract: The early interactions between the nasal epithelial layer and the innate immune cells during viral infections remains an under-explored area. The significance of innate immunity signaling in viral infections has increased substantially as patients with ... ...
| Abstract | The early interactions between the nasal epithelial layer and the innate immune cells during viral infections remains an under-explored area. The significance of innate immunity signaling in viral infections has increased substantially as patients with respiratory infections who exhibit high innate T cell activation show a better disease outcome. Hence, dissecting these early innate immune interactions allows the elucidation of the processes that govern them and may facilitate the development of potential therapeutic targets and strategies for dampening or even preventing early progression of viral infections. This protocol details a versatile model that can be used to study early crosstalk, interactions, and activation of innate immune cells from factors secreted by virally infected airway epithelial cells. Using an H3N2 influenza virus (A/Aichi/2/1968) as the representative virus model, innate cell activation of co-cultured peripheral blood mononuclear cells (PBMCs) has been analyzed using flow cytometry to investigate the subsets of cells that are activated by the soluble factors released from the epithelium in response to the viral infection. The results demonstrate the gating strategy for differentiating the subsets of cells and reveal the clear differences between the activated populations of PBMCs and their crosstalk with the control and infected epithelium. The activated subsets can then be further analyzed to determine their functions as well as molecular changes specific to the cells. Findings from such a crosstalk investigation may uncover factors that are important for the activation of vital innate cell populations, which are beneficial in controlling and suppressing the progression of viral infection. Furthermore, these factors can be universally applied to different viral diseases, especially to newly emerging viruses, to dampen the impact of such viruses when they first circulate in naïve human populations. |
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| MeSH term(s) | 3T3 Cells ; Animals ; Cell Differentiation/drug effects ; Cells, Cultured ; Coculture Techniques ; Electric Impedance ; Epithelial Cells/drug effects ; Epithelial Cells/immunology ; Feeder Cells/cytology ; Humans ; Immunity, Innate ; Influenza A Virus, H3N2 Subtype/drug effects ; Influenza A Virus, H3N2 Subtype/immunology ; Influenza, Human/immunology ; Influenza, Human/virology ; Killer Cells, Natural/drug effects ; Killer Cells, Natural/immunology ; Leukocytes, Mononuclear/drug effects ; Leukocytes, Mononuclear/virology ; Mice ; Mitomycin/pharmacology ; Models, Biological ; Mucin 5AC/metabolism ; Nasal Mucosa/pathology ; Tubulin/metabolism |
| Chemical Substances | MUC5AC protein, human ; Mucin 5AC ; Tubulin ; Mitomycin (50SG953SK6) |
| Language | English |
| Publishing date | 2021-02-28 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't ; Video-Audio Media |
| ZDB-ID | 2259946-0 |
| ISSN | 1940-087X ; 1940-087X |
| ISSN (online) | 1940-087X |
| ISSN | 1940-087X |
| DOI | 10.3791/62115 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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