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  1. Book: Success factors for fish larval production

    Conceição, Luís E. C. / Tandler, Amos

    2018  

    Author's details edited by Luís E. C. Conceição and Amos Tandler
    Keywords Aquaculture ; Aquaculture, Fisheries & Fish Science ; Aquakultur ; Aquakultur, Fischereiwesen u. Fischforschung ; Biowissenschaften ; Developmental Biology ; Entwicklungsbiologie ; Fischzucht ; Life Sciences ; AQ10: Aquakultur ; LS03: Entwicklungsbiologie
    Subject code 630
    Language English
    Size xvii, 494 Seiten, 24.4 cm x 17 cm
    Publisher Wiley Blackwell
    Publishing place Hoboken, NJ ; Chichester
    Publishing country United States
    Document type Book
    HBZ-ID HT019430490
    ISBN 978-1-119-07216-4 ; 1-119-07216-6 ; 9781119072140 ; 9781119072133 ; 111907214X ; 1119072131
    Database Catalogue ZB MED Nutrition, Environment, Agriculture

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  2. Article ; Online: Development of a Rainbow Trout (Oncorhynchus mykiss) Intestinal In Vitro Platform for Profiling Amino Acid Digestion and Absorption of a Complete Diet

    Pasquariello, Rolando / Pavlovic, Radmila / Chacon, Marcelo A. / Camin, Federica / Verdile, Nicole / Løkka, Guro / Panseri, Sara / Faustini, Massimo / Tandler, Amos / Peggs, David / Kortner, Trond M. / Bitan, Amir / Brevini, Tiziana A. L. / Gandolfi, F.

    Animals. 2023 July 12, v. 13, no. 14

    2023  

    Abstract: The ever-increasing number and variation of raw materials utilized to provide alternative feed formulations continues to allow for a more sustainable and flexible approach. Testing all these options in vivo is still the most robust and reliable manner to ...

    Abstract The ever-increasing number and variation of raw materials utilized to provide alternative feed formulations continues to allow for a more sustainable and flexible approach. Testing all these options in vivo is still the most robust and reliable manner to pick the best raw material candidates, but it requires the use of large numbers of animals and is time-consuming and expensive. Therefore, we are developing an in vitro platform that can provide a reliable evaluation of new ingredients. The main aim of this work was to combine an in vitro digestion protocol of extruded, commercially relevant aquafeeds with the exposure of intestinal epithelial cells to the extracted bio-available fraction (BAF). The results show that 250,000 cells/cm² represents the optimal seeding density and that up to 50% BAF concentration for up to 24 h had no negative effects on the epithelial barrier morphology and function. It is possible to determine amino acid digestibility and bioavailability in all the experimental conditions (with and without BSA, at 25% and 50% dilution) and at all time points (0, 6, and 24 h). However, BAF concentration, the medium used for its dilution, and the length of exposure to the different epithelial cell lines can all influence the results and, therefore, must be selected according to the final aim of the experiment.
    Keywords Oncorhynchus mykiss ; absorption ; amino acids ; aquaculture feeds ; bioavailability ; diet ; digestible amino acids ; digestion ; epithelial cells ; epithelium ; intestines ; raw materials ; wet digestion method
    Language English
    Dates of publication 2023-0712
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani13142278
    Database NAL-Catalogue (AGRICOLA)

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  3. Article: Development of a Rainbow Trout (

    Pasquariello, Rolando / Pavlovic, Radmila / Chacon, Marcelo A / Camin, Federica / Verdile, Nicole / Løkka, Guro / Panseri, Sara / Faustini, Massimo / Tandler, Amos / Peggs, David / Kortner, Trond M / Bitan, Amir / Brevini, Tiziana A L / Gandolfi, Fulvio

    Animals : an open access journal from MDPI

    2023  Volume 13, Issue 14

    Abstract: The ever-increasing number and variation of raw materials utilized to provide alternative feed formulations continues to allow for a more sustainable and flexible approach. Testing all these ... ...

    Abstract The ever-increasing number and variation of raw materials utilized to provide alternative feed formulations continues to allow for a more sustainable and flexible approach. Testing all these options
    Language English
    Publishing date 2023-07-12
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani13142278
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: The effect of dissolved carbon dioxide (CO2) on the bone mineral content and on the expression of bone-Gla protein (BGP, Osteocalcin) in the vertebral column of white grouper (Epinephelus aeneus)

    Elsadin, Suliman / Nixon, Oriya / Mozes, Noam / Allon, Guy / Gaon, Aviad / Tandler, Amos / Koven, William

    Aquaculture. 2019 June 04,

    2019  

    Abstract: The aim of this study was to test the effect of long-term dissolved CO2 exposure on white grouper (Epinephelus aeneus). 45 day post hatching (dph) groupers (0.4 ± 0.05 g; 2.1 ± 0.1 cm) were equally distributed to 15 aquaria (17 L) at a density of 40 ... ...

    Abstract The aim of this study was to test the effect of long-term dissolved CO2 exposure on white grouper (Epinephelus aeneus). 45 day post hatching (dph) groupers (0.4 ± 0.05 g; 2.1 ± 0.1 cm) were equally distributed to 15 aquaria (17 L) at a density of 40 larvae per aquarium. The fish were grown for 60 days at a salinity of 25 ppt (26.5 οC) while being exposed to three dissolved CO2 concentrations: Control (0.8 ± 0.1 mg L−1; pH 7.9 ± 0.1), Medium (5.5 ± 0.2 mg L−1; pH 7.1 ± 0.1) and High (28.5 ± 1.5 mg L−1; pH 6.2 ± 0.1). Analysis of bone mineral contents showed that at the end of the CO2 exposure period (105 dph), the Ca levels were significantly higher (P < .001) in the skeleton of fish from the high CO2 treatment as compared to the medium and control treatments. However, the P levels were not significantly different between the three treatments (P > .05). The gene expression of bone Gla protein (BGP, Osteocalcin), a marker for skeletal mineralization, was significantly higher in the vertebral column of the fish from the control treatment as compared to the medium (P < .05) and high (P < .01) treatments. The expression of BGP mRNA was positively correlated with the fish growth rate, as the fish from the control treatment presented the highest body weight at the end of the experiment.
    Keywords Epinephelus aeneus ; animal growth ; aquariums ; body weight ; calcium ; dissolved carbon dioxide ; exposure duration ; gene expression ; grouper ; hatching ; larvae ; messenger RNA ; mineral content ; mineralization ; osteocalcin ; pH ; salinity ; skeleton ; spine (bones)
    Language English
    Dates of publication 2019-0604
    Publishing place Elsevier B.V.
    Document type Article
    Note Pre-press version
    ZDB-ID 185380-6
    ISSN 0044-8486 ; 0044-8516
    ISSN 0044-8486 ; 0044-8516
    DOI 10.1016/j.aquaculture.2019.06.010
    Database NAL-Catalogue (AGRICOLA)

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  5. Article ; Online: The effect of dissolved carbon dioxide (CO2) on the bone mineral content and on the expression of bone-Gla protein (BGP, Osteocalcin) in the vertebral column of white grouper (Epinephelus aeneus)

    Elsadin, Suliman / Nixon, Oriya / Mozes, Noam / Allon, Guy / Gaon, Aviad / Tandler, Amos / Koven, William

    2019  

    Abstract: The aim of this study was to test the effect of long-term dissolved CO2 exposure on white grouper (Epinephelus aeneus). 45 day post hatching (dph) groupers (0.4 ± 0.05 g; 2.1 ± 0.1 cm) were equally distributed to 15 aquaria (17 L) at a density of 40 ... ...

    Abstract The aim of this study was to test the effect of long-term dissolved CO2 exposure on white grouper (Epinephelus aeneus). 45 day post hatching (dph) groupers (0.4 ± 0.05 g; 2.1 ± 0.1 cm) were equally distributed to 15 aquaria (17 L) at a density of 40 larvae per aquarium. The fish were grown for 60 days at a salinity of 25 ppt (26.5 οC) while being exposed to three dissolved CO2 concentrations: Control (0.8 ± 0.1 mg L−1; pH 7.9 ± 0.1), Medium (5.5 ± 0.2 mg L−1; pH 7.1 ± 0.1) and High (28.5 ± 1.5 mg L−1; pH 6.2 ± 0.1). Analysis of bone mineral contents showed that at the end of the CO2 exposure period (105 dph), the Ca levels were significantly higher (P < .001) in the skeleton of fish from the high CO2 treatment as compared to the medium and control treatments. However, the P levels were not significantly different between the three treatments (P > .05). The gene expression of bone Gla protein (BGP, Osteocalcin), a marker for skeletal mineralization, was significantly higher in the vertebral column of the fish from the control treatment as compared to the medium (P < .05) and high (P < .01) treatments. The expression of BGP mRNA was positively correlated with the fish growth rate, as the fish from the control treatment presented the highest body weight at the end of the experiment.
    Subject code 630
    Language English
    Publisher Elsevier
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article: The effect of algal turbidity on larval performance and the ontogeny of digestive enzymes in the grey mullet (Mugil cephalus)

    Koven, William / Gisbert, Enric / Nixon, Oriya / Solovyev, Mikhail M / Gaon, Aviad / Allon, Guy / Meiri-Ashkenazi, Iris / Tandler, Amos / Rosenfeld, Hanna

    Comparative biochemistry and physiology. 2019 Feb., v. 228

    2019  

    Abstract: A study comprised of two trials determined the effects of water turbidity produced by live microalgae and inert clay particles on the larval rearing of grey mullet (Mugil cephalus). Trial 1 evaluated the effect of microalgae produced water turbidity on ... ...

    Abstract A study comprised of two trials determined the effects of water turbidity produced by live microalgae and inert clay particles on the larval rearing of grey mullet (Mugil cephalus). Trial 1 evaluated the effect of microalgae produced water turbidity on grey mullet larval performance and digestive tract (DT) enzyme ontogeny. Two microalgae (Nannochloropsis oculata and Isochrysis galbana) water turbidity levels (0.76 and 1.20 NTU, respectively) and a non-microalgae control (0.26 NTU) were investigated on 2 to 23 dph grey mullet larvae. The higher turbidity (1.2 NTU) larvae (5 dph) consumed markedly (P < .05) more rotifers than other treatment fish, independently of the microalgae type. There was no clear effect of the turbidity treatments on DT enzyme ontogeny. However, in all treatments lipase and alkaline proteases appeared to be modulated by the diet. Alkaline phosphatase activity was ca. 8 times higher and α-amylase activity increased 5.3 times in 79 dph fish compared to 40 dph individuals. The ratio of alkaline phosphatase and leucine-alanine aminopeptidase indicated gut maturation occurred around 61 dph. Trial 2 compared the most effective N.occulata produced turbidity level (1.2 NTU) with the identical water turbidity produced by inert clay on larval performance. M. cephalus larvae exposed to high algal turbidity demonstrated superior performance (P < .05), in terms of rotifer ingestion, dry weight gain and survival, compared to cohorts reared under the clay treatment and the lower microalgae produced turbidity. These findings suggested that water algal turbidity is not the dominant factor determining improved grey mullet larval performance.
    Keywords Isochrysis galbana ; Mugil cephalus ; Nannochloropsis ; Rotifera ; alkaline phosphatase ; alpha-amylase ; aminopeptidases ; carboxylic ester hydrolases ; clay ; diet ; digestive tract ; enzyme activity ; fish ; ingestion ; larvae ; microalgae ; ontogeny ; proteinases ; rearing ; turbidity ; weight gain
    Language English
    Dates of publication 2019-02
    Size p. 71-80.
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 121246-1
    ISSN 1531-4332 ; 0300-9629 ; 1095-6433
    ISSN (online) 1531-4332
    ISSN 0300-9629 ; 1095-6433
    DOI 10.1016/j.cbpa.2018.11.005
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: The effect of algal turbidity on larval performance and the ontogeny of digestive enzymes in the grey mullet (Mugil cephalus).

    Koven, William / Gisbert, Enric / Nixon, Oriya / Solovyev, Mikhail M / Gaon, Aviad / Allon, Guy / Meiri-Ashkenazi, Iris / Tandler, Amos / Rosenfeld, Hanna

    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

    2018  Volume 228, Page(s) 71–80

    Abstract: A study comprised of two trials determined the effects of water turbidity produced by live microalgae and inert clay particles on the larval rearing of grey mullet (Mugil cephalus). Trial 1 evaluated the effect of microalgae produced water turbidity on ... ...

    Abstract A study comprised of two trials determined the effects of water turbidity produced by live microalgae and inert clay particles on the larval rearing of grey mullet (Mugil cephalus). Trial 1 evaluated the effect of microalgae produced water turbidity on grey mullet larval performance and digestive tract (DT) enzyme ontogeny. Two microalgae (Nannochloropsis oculata and Isochrysis galbana) water turbidity levels (0.76 and 1.20 NTU, respectively) and a non-microalgae control (0.26 NTU) were investigated on 2 to 23 dph grey mullet larvae. The higher turbidity (1.2 NTU) larvae (5 dph) consumed markedly (P < .05) more rotifers than other treatment fish, independently of the microalgae type. There was no clear effect of the turbidity treatments on DT enzyme ontogeny. However, in all treatments lipase and alkaline proteases appeared to be modulated by the diet. Alkaline phosphatase activity was ca. 8 times higher and α-amylase activity increased 5.3 times in 79 dph fish compared to 40 dph individuals. The ratio of alkaline phosphatase and leucine-alanine aminopeptidase indicated gut maturation occurred around 61 dph. Trial 2 compared the most effective N.occulata produced turbidity level (1.2 NTU) with the identical water turbidity produced by inert clay on larval performance. M. cephalus larvae exposed to high algal turbidity demonstrated superior performance (P < .05), in terms of rotifer ingestion, dry weight gain and survival, compared to cohorts reared under the clay treatment and the lower microalgae produced turbidity. These findings suggested that water algal turbidity is not the dominant factor determining improved grey mullet larval performance.
    MeSH term(s) Animals ; Diet ; Digestive System/enzymology ; Enzymes/classification ; Enzymes/metabolism ; Female ; Larva/physiology ; Male ; Nephelometry and Turbidimetry ; Rotifera ; Smegmamorpha/growth & development
    Chemical Substances Enzymes
    Language English
    Publishing date 2018-11-12
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 121246-1
    ISSN 1531-4332 ; 0300-9629 ; 1095-6433
    ISSN (online) 1531-4332
    ISSN 0300-9629 ; 1095-6433
    DOI 10.1016/j.cbpa.2018.11.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Improved performance of gilthead sea bream, Sparus aurata, larvae after ozone disinfection of the eggs

    Ben-Atia, Isashar / Lutzky, Sigal / Barr, Yoav / Gamsiz, Kutsal / Shtupler, Yariv / Tandler, Amos / Koven, William

    Aquaculture research. 2007 Feb., v. 38, no. 2

    2007  

    Abstract: Ozone (O₃) dissolved in seawater (ODS) was evaluated, as an egg disinfectant, on the spawn of captive gilthead sea bream, Sparus aurata, brood stock. Four contact times (CT) were tested (0.6, 1.2, 2.4 and 4.8 mg min L⁻¹) where CT was calculated by ... ...

    Abstract Ozone (O₃) dissolved in seawater (ODS) was evaluated, as an egg disinfectant, on the spawn of captive gilthead sea bream, Sparus aurata, brood stock. Four contact times (CT) were tested (0.6, 1.2, 2.4 and 4.8 mg min L⁻¹) where CT was calculated by multiplying the dissolved O₃ concentration (0.3 mg L⁻¹) by different exposure periods (2, 4, 8, 16 min). There was also a disinfected seawater treatment that contained no O₃ or derived compounds (CT 0) and an untreated seawater control. All ODS treatments reduced egg surface bacterial counts to zero, which was significantly (P<0.05) lower than the CT 0 and the control groups (194 and 1320 plate⁻¹ respectively). Nevertheless, the hatching rate was high in the control and the CT treatments 0, 0.6 and 1.2 (88.7%, 87.3%, 89.5% and 83.7% respectively) while eggs exposed to a CT 2.4 and 4.8 hatched poorly (36.5% and 20.4% respectively), which was likely due, at least in part, to larvae unable to break the egg chorion successfully. Swim-bladder inflation was significantly higher in the ODS groups (>97%) compared with the control and CT 0 treatments (ca. 70%). The results suggest that a 2-min exposure of eggs to 0.3 mg O₃ L⁻¹ of ODS (CT 0.6) would improve current protocols in marine larviculture.
    Keywords ozone ; fish larvae ; chorion
    Language English
    Dates of publication 2007-02
    Size p. 166-173.
    Publisher Blackwell Publishing Ltd
    Publishing place Oxford, UK
    Document type Article
    ZDB-ID 1227359-4
    ISSN 1355-557X
    ISSN 1355-557X
    DOI 10.1111/j.1365-2109.2006.01641.x
    Database NAL-Catalogue (AGRICOLA)

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  9. Book: Rotifer resting eggs and their application to marine aquaculture

    Lubzens, Esther / Tandler, Amos / Snell, Terry W

    1985  

    Institution United States-Israel Binational Agricultural Research and Development Fund
    Author's details Esther Lubzens, Amos Tandler, Terry W. Snell. --
    Keywords Rotifera/Eggs. ; Rotifera/Genetics. ; Fishes/Larvae. ; Mariculture.
    Language English
    Size 1 v. (various foliations) :, ill. ;, 29 cm.
    Publisher BARD
    Publishing place Bet Dagan, Israel
    Document type Book
    Note Final report. ; Project no. I-186.
    Database NAL-Catalogue (AGRICOLA)

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