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Article ; Online: Establishing a 3D culture system for early organogenesis of monkey embryos ex vivo and single-cell transcriptome analysis of cultured embryos

Nianqin Sun / Honglian Shao / Youyue Zhang / Baiquan Ci / Hui Yao / Bing Bai / Tao Tan

STAR Protocols, Vol 5, Iss 1, Pp 102835- (2024)

2024

Abstract: Summary: Creating in vitro culture platforms for monkey embryos is crucial for understanding the initial 4 weeks of early primate embryogenesis. Here, we present a protocol to culture cynomolgus monkey embryos in vitro for 25 days post-fertilization and ... ...

Abstract	Summary: Creating in vitro culture platforms for monkey embryos is crucial for understanding the initial 4 weeks of early primate embryogenesis. Here, we present a protocol to culture cynomolgus monkey embryos in vitro for 25 days post-fertilization and to delineate the key developmental events of gastrulation and early organogenesis. We describe steps for culturing with a 3D system, immunofluorescence analysis, single-cell RNA sequencing, and bioinformatic analysis.For complete details on the use and execution of this protocol, please refer to Gong et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
Keywords	Bioinformatics ; Sequence analysis ; Cell Biology ; Cell culture ; Cell isolation ; Single Cell ; Science (General) ; Q1-390
Language	English
Publishing date	2024-03-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Salvinorin A inhibits ovalbumin‐stimulated allergic rhinitis and RBL‐2H3 cells degranulation

Qiyang Shou / Tao Tan / Faying Xu

FEBS Open Bio, Vol 11, Iss 8, Pp 2166-

2021 Volume 2173

Abstract: Allergic rhinitis (AR) is a long‐term noncommunicable inflammatory disease of the nasal mucosa mediated by immunoglobulin E and is mainly caused by exposure of genetically susceptible individuals to environmental allergens. Mast cells contribute to the ... ...

Abstract	Allergic rhinitis (AR) is a long‐term noncommunicable inflammatory disease of the nasal mucosa mediated by immunoglobulin E and is mainly caused by exposure of genetically susceptible individuals to environmental allergens. Mast cells contribute to the pathogenesis of allergic and nonallergic inflammatory diseases. Salvinorin A has been previously shown to inhibit leukotriene production and mast cell degranulation to suppress airway hyperresponsiveness caused by sensitization; thus, we hypothesized that salvinorin A has an anti‐AR effect. We tested this hypothesis using monoclonal anti‐2,4,6‐dinitrophenyl immunoglobulin E/human serum albumin‐induced rat basophilic leukemia cells (RBL‐2H3 cells) and ovalbumin (OVA)‐induced AR in mice as in vivo and in vitro AR models, respectively. The expression levels of histamine, β‐hexosaminidase, interleukin‐4 and tumor necrosis factor‐α were decreased by salvinorin A in vitro. Granule release and F‐actin organization were also suppressed by salvinorin A. Furthermore, salvinorin A inhibited OVA‐induced features of AR in mice, including nasal rubbing and sneezing, as well as increased OVA‐specific immunoglobulin E, histamine, tumor necrosis factor‐α and interleukin‐4 levels. In addition, salvinorin A decreased the phosphorylation of phosphoinositide 3‐kinase/Akt in vitro and in vivo. Our work suggests that salvinorin A suppresses AR caused by sensitization by inhibiting the inflammatory responses of mast cells; thus, salvinorin A may have potential for treatment of AR.
Keywords	allergic rhinitis ; inflammation ; mast cells ; ovalbumin ; salvinorin A ; Biology (General) ; QH301-705.5
Subject code	610
Language	English
Publishing date	2021-08-01T00:00:00Z
Publisher	Wiley
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Mapping of the Greenhouse Gas Emission Potential for the Offshore Wind Power Sector in Guangdong, China

Zetao Huang / Youkai Yu / Yushu Chen / Tao Tan / Xuhui Kong

Sustainability, Vol 14, Iss 15533, p

2022 Volume 15533

Abstract: This study aims to assess the potential greenhouse gas (GHG) emissions of delivering 1 kWh from planned offshore wind farm sites to the grid in the Guangdong Province, China. In contrast to most previous studies, we avoided underestimating GHG emissions ... ...

Abstract	This study aims to assess the potential greenhouse gas (GHG) emissions of delivering 1 kWh from planned offshore wind farm sites to the grid in the Guangdong Province, China. In contrast to most previous studies, we avoided underestimating GHG emissions per kWh by approximately 49% by adopting a spatialized life-cycle inventory (LCI)-improved stock-driven model under the medium scenario combination. We also developed a callable spatialized LCI to model the spatial differences in the GHG emissions per kWh by cells in planned offshore wind farm sites in Guangdong. The modeling results indicate that, under the medium scenario combination, the GHG emissions per kWh will range from 4.6 to 19 gCO 2eq /kWh and the cells with higher emissions are concentrated in the deep-water wind farms in the eastern ocean of the Guangdong Province. According to the mechanism by which the different scenarios affect the modeling results, increasing the unit capacity of turbines is the most effective approach for reducing the GHG emissions per kWh and decreasing the impact of natural conditions. Air density can be used as an empirical spatial variable to predict the GHG emission potential of planned wind farm sites in Guangdong. The modeling framework in this study provides a more reliable quantitative tool for decision-makers in the offshore wind sector that can be used directly for any offshore wind system with a monopile foundation and be extended to wind power systems with other foundation types, or even to the entire renewable energy and other infrastructure systems after certain modifications.
Keywords	dynamic material flow analysis ; life-cycle assessment ; climate change ; carbon neutral ; spatial analysis ; Environmental effects of industries and plants ; TD194-195 ; Renewable energy sources ; TJ807-830 ; Environmental sciences ; GE1-350
Subject code	551
Language	English
Publishing date	2022-11-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Improving Outcomes in the Advanced Gastrointestinal Stromal Tumors

Pan Ran / Hui Zhou / Jinjin Li / Tao Tan / Hao Yang / Juan Li / Jun Zhang

Journal of Personalized Medicine, Vol 13, Iss 417, p

The Role of the Multidisciplinary Team Discussion Intervention

2023 Volume 417

Abstract: Objectives: There is disagreement over the prognostic value of multidisciplinary team (MDT) discussion for advanced gastrointestinal stromal tumors (GISTs). This study examined how an MDT affected patients with advanced GISTs in terms of their overall ... ...

Abstract	Objectives: There is disagreement over the prognostic value of multidisciplinary team (MDT) discussion for advanced gastrointestinal stromal tumors (GISTs). This study examined how an MDT affected patients with advanced GISTs in terms of their overall survival (OS) and whether it may enhance their performance status (PS). Methods: A retrospective data analysis was conducted on patients with advanced GISTs between 2000 and 2022. Depending on whether they had received the MDT discussion intervention, the patients were split into two groups. The OS between the two groups was compared using the Kaplan–Meier method. A multivariate Cox regression analysis was used to analyze the prognostic variables for advanced GIST. Fisher’s test was used to investigate the relationship between an MDT and PS. Results: There were 122 patients with an MDT and 117 patients without an MDT in this study. In comparison to the non-MDT group, the MDT group showed a higher survival rate (5-year OS, 42.62% vs. 28.21%, p < 0.05). MDT was an independent prognostic factor for OS in univariate and multivariate Cox regression analyses ( p < 0.05). Fisher’s test revealed that there were variations in PS between the two groups ( p < 0.05). Conclusions: The effectiveness of an MDT in the treatment of advanced GIST was examined for the first time in this study. MDT discussion intervention is an effective measure for improving the outcomes of patients with advanced GISTs.
Keywords	gastrointestinal stromal tumor ; multidisciplinary team ; overall survival ; performance status ; prognosis ; Medicine ; R
Subject code	616 ; 796
Language	English
Publishing date	2023-02-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Nomogram for Predicting Recurrence-Free Survival of Primary Localized Gastrointestinal Stromal Tumor

Pan Ran / Tao Tan / Hui Zhou / Jinjin Li / Hao Yang / Juan Li / Jun Zhang

Journal of Personalized Medicine, Vol 13, Iss 498, p

2023 Volume 498

Abstract: Purpose: This study aimed to establish a new nomogram that predicts recurrence-free survival (RFS) after a complete surgical resection of primary localized gastrointestinal stromal tumors (GISTs); it also aimed to evaluate the discrimination, calibration, ...

Abstract	Purpose: This study aimed to establish a new nomogram that predicts recurrence-free survival (RFS) after a complete surgical resection of primary localized gastrointestinal stromal tumors (GISTs); it also aimed to evaluate the discrimination, calibration, and clinical utility of the decision-making nomogram. Methods: The clinicopathological data of patients with primary localized GISTs at the First Affiliated Hospital of Chongqing Medical University from January 2000 to June 2022 were retrospectively analyzed. The clinicopathological data were randomly split into two sets (7:3 ratio) for training and validation. Suitable variables for the construction of a nomogram for the 1-, 3-, and 5-year RFS were selected using univariate and multivariate Cox regression analyses. Receiver operating characteristic (ROC) analysis and a concordance index (C-index) were used to quantify the discrimination of the nomogram and were compared with four commonly used prognostic scoring systems: Memorial Sloan Kettering Cancer Center prognostic nomogram, National Institutes of Health–Fletcher staging system, Chen’s prognostic nomogram, and Air Forces Institute of Pathology risk criteria–Miettinen staging system. The calibration and clinical utility for the decision-making nomogram were validated using calibration curves and decision curves, respectively. Results: In total, 641 patients were screened and analyzed in this retrospective, observational study. RFS was significantly related to tumor size, mitotic count, gender, DOG-1, and adjuvant therapy with imatinib according to the results of the multivariate and univariate Cox analyses. The nomogram was constructed using the above variables (all p < 0.05) for the 1-, 3-, and 5-year RFS. In the training set, the 1-, 3-, and 5-year ROC and C-index values of the nomogram were 0.868, 0.838, 0.816, and 0.830, respectively. For internal validation, we performed model fitting on the validation set, and the 1-, 3-, and 5-year ROC and C-indices were 0.977, 0.845, 0.869, and 0.849, ...
Keywords	recurrence-free survival ; gastrointestinal stromal tumor ; nomogram ; individualized treatment ; prediction ; Medicine ; R
Language	English
Publishing date	2023-03-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Failure characteristics and the law of the energy evolution of granite with different pre-crack inclination angles under uniaxial compression loading

Ercheng Zhao / Juhong Li / Xiongtian Zhang / Chunyang Zhang / Qinglin Ren / Tao Tan / Yixian Wang

Frontiers in Earth Science, Vol

2023 Volume 11

Abstract: Cracks affect the strength of rock masses and eventually threaten their stability in engineering. In order to study the fracture characteristics and mechanical properties of cracked rocks, uniaxial compression tests of pre-cracked granite samples with a ... ...

Abstract	Cracks affect the strength of rock masses and eventually threaten their stability in engineering. In order to study the fracture characteristics and mechanical properties of cracked rocks, uniaxial compression tests of pre-cracked granite samples with a central circular through hole were carried out by using MTS816 rock mechanics testing system. The inclination angles of different pre-cracks are 0°, 15°, 30°, 45°, 60°, 75°, and 90° respectively, and the influence of the crack stop hole near the crack tip on the failure behavior of pre-cracked samples is also considered. The results indicate that, compared with the intact sample, the peak strength of pre-cracked samples decreases significantly and is related to the pre-crack inclination angle. The failure mode of the sample varies with the pre-crack inclination angle, and the crack stop hole near the crack tip also has a certain influence on the crack growth to a certain extent. However, in terms of failure mode and its transformation law, the influence of central circular through hole and crack stop hole can be ignored. Generally speaking, the larger the inclination angle of the pre-crack, the more the total energy required for failure of the sample, and the more the stored elastic strain energy. Before the peak strength, the elastic strain energy of the sample is greater than the dissipated energy, after the peak strength, the dissipated energy gradually exceeds the elastic strain energy due to energy conversion. It is found that the pre-crack reduces the energy storage capacity of the sample, and the total energy is ultimately dominated by sample integrity. The dissipated energy rate increases first, then decreases, and finally increases again, the inflection points are the end of micro-crack closure and the peak strength, respectively. The crack stop hole changes the law of energy evolution to a certain extent, which can improve the ability of rocks to accumulate energy when designed at an appropriate position, so as to improve its load-bearing capacity in a ...
Keywords	pre-cracked granite sample ; central circular through hole ; crack stop hole ; uniaxial compression test ; energy evolution ; mechanical properties ; Science ; Q
Subject code	669
Language	English
Publishing date	2023-06-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Cadmium (Cd) exposure through Hyphantria cunea pupae reduces the parasitic fitness of Chouioia cunea

Shan-chun YAN / Hong-fei WU / Lin ZHENG / Ming-tao TAN / Dun JIANG

Journal of Integrative Agriculture, Vol 22, Iss 10, Pp 3103-

A potential risk to its biocontrol efficiency

2023 Volume 3114

Abstract: Heavy metal contamination has been regarded as an environmental variable that affects the efficiency of pest biological control, but the parasitic fitness of parasitoids under heavy metal stress is poorly understood. Herein, the effect of Cd exposure ... ...

Abstract	Heavy metal contamination has been regarded as an environmental variable that affects the efficiency of pest biological control, but the parasitic fitness of parasitoids under heavy metal stress is poorly understood. Herein, the effect of Cd exposure through the host pupa of Hyphantria cunea on the parasitic fitness of Chouioia cunea was investigated, and the mechanism by which Cd exposure affects the interaction between H. cunea and C. cunea from the perspective of innate immunity in host insect and the oxidative status in the parasitoid offspring was explored. Our results indicated that Cd can be transferred from the H. cunea pupae to the parasitoid offspring, and the transfer coefficient reflected biological amplification. There were no significant differences in the rates of parasitism success and offspring emergence between the untreated and Cd-treated groups. However, after parasitizing Cd-accumulated pupae, the parasitic fitness of offspring wasps (e.g., the number, individual size and life span) decreased significantly. Under Cd exposure, the cellular and humoral immunity of H. cunea pupae decreased significantly. Compared with the untreated group, the H2O2 content of parasitoid offspring in the Cd-treated group was significantly increased. Cd exposure significantly inhibited superoxide dismutase activity in parasitoid offspring, but the contents of ascorbic acid and glutathione were significantly increased by Cd stress. Taken together, these results indicate that Cd exposure reduces the cyclic utilization efficiency of C. cunea on H. cunea pupae. The oxidative status of parasitoid offspring triggered by Cd exposure could be responsible for the reduced parasitic fitness of C. cunea on Cd-accumulated H. cunea pupae.
Keywords	heavy metal ; Hyphantria cunea ; parasitic fitness ; Chouioia cunea ; oxidative status ; innate immunity ; Agriculture (General) ; S1-972
Language	English
Publishing date	2023-10-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Focus-RCNet

Dashun Zheng / Rongsheng Wang / Yaofei Duan / Patrick Cheong-Iao Pang / Tao Tan

Visual Computing for Industry, Biomedicine, and Art, Vol 6, Iss 1, Pp 1-

a lightweight recyclable waste classification algorithm based on focus and knowledge distillation

2023 Volume 9

Abstract: Abstract Waste pollution is a significant environmental problem worldwide. With the continuous improvement in the living standards of the population and increasing richness of the consumption structure, the amount of domestic waste generated has ... ...

Abstract	Abstract Waste pollution is a significant environmental problem worldwide. With the continuous improvement in the living standards of the population and increasing richness of the consumption structure, the amount of domestic waste generated has increased dramatically, and there is an urgent need for further treatment. The rapid development of artificial intelligence has provided an effective solution for automated waste classification. However, the high computational power and complexity of algorithms make convolutional neural networks unsuitable for real-time embedded applications. In this paper, we propose a lightweight network architecture called Focus-RCNet, designed with reference to the sandglass structure of MobileNetV2, which uses deeply separable convolution to extract features from images. The Focus module is introduced to the field of recyclable waste image classification to reduce the dimensionality of features while retaining relevant information. To make the model focus more on waste image features while keeping the number of parameters small, we introduce the SimAM attention mechanism. In addition, knowledge distillation was used to further compress the number of parameters in the model. By training and testing on the TrashNet dataset, the Focus-RCNet model not only achieved an accuracy of 92 $$\%$$ % but also showed high deployment mobility.
Keywords	Waste recycling ; Waste classification ; Knowledge distillation ; Lightweight ; Attention ; Drawing. Design. Illustration ; NC1-1940 ; Computer applications to medicine. Medical informatics ; R858-859.7 ; Computer software ; QA76.75-76.765
Subject code	006
Language	English
Publishing date	2023-10-01T00:00:00Z
Publisher	SpringerOpen
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Book ; Online: Dataset of search trends-based analysis of public attention in the early outbreak of COVID-19 in China.xlsx

Tao Tan (6527945) / Zetao Huang (9021386)

2020

Abstract: Statistics results of Baidu index and Google trends on keywords related to the epidemic from January 10 to February ... ...

Abstract	Statistics results of Baidu index and Google trends on keywords related to the epidemic from January 10 to February 28
Keywords	Epidemiology ; COVID_19 ; Baidu index ; google trends ; covid19
Publishing date	2020-06-24T11:53:49Z
Publishing country	us
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Book ; Online: Dataset of search trends-based analysis of public attention in the early outbreak of COVID-19 in China

Tao Tan (9021795) / Zetao Huang (9021386)

2020

Abstract: Statistics results of Baidu index and Google trends on keywords related to the epidemic from January 10 to February ... ...

Abstract	Statistics results of Baidu index and Google trends on keywords related to the epidemic from January 10 to February 28
Keywords	Epidemiology ; COVID-19 virus (SARS-CoV-2) ; Baidu index ; Google trends ; covid19
Publishing date	2020-06-24T12:25:28Z
Publishing country	us
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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