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  1. Article: Medicinal plant extracts interfere in gastric cancer stem cells fluorescence-based assays.

    Tapia-Rojas, Salyoc / García-Paitán, Marlon / Rosario-Chavarri, Jorge Del / Santiani, Alexei / Alvarez-Vega, Santiago / Amiel-Pérez, José / Mayanga-Herrera, Ana

    Saudi journal of biological sciences

    2024  Volume 31, Issue 6, Page(s) 104000

    Abstract: Fluorescence is used in various biological assays due to its high sensitivity, versatility, and precision. In recent years, studies using medicinal plant extracts have increased. However, fluorescence-based assays could be biased by plant metabolites ... ...

    Abstract Fluorescence is used in various biological assays due to its high sensitivity, versatility, and precision. In recent years, studies using medicinal plant extracts have increased. However, fluorescence-based assays could be biased by plant metabolites autofluorescence. To address this issue, this study investigated the interference caused by methanolic extracts and chloroform fractions of three medicinal plants in three fluorescence-based assays on gastric cancer stem cells(CSC): resazurin reduction, confocal microscopy, and flow cytometry. CSC were isolated based on CD44 surface marker, incubated with methanolic extracts and chloroform fractions of
    Language English
    Publishing date 2024-04-15
    Publishing country Saudi Arabia
    Document type Journal Article
    ZDB-ID 2515206-3
    ISSN 2213-7106 ; 1319-562X
    ISSN (online) 2213-7106
    ISSN 1319-562X
    DOI 10.1016/j.sjbs.2024.104000
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Procedimiento para el cultivo e identificación de células madre obtenidas de lipoaspirado humano con fines de investigación.

    Tapia-Rojas, Salyoc / Mayanga-Herrera, Ana / Enciso-Gutiérrez, Javier / Centurion, Patricio / Amiel-Pérez, José

    Revista peruana de medicina experimental y salud publica

    2020  Volume 37, Issue 3, Page(s) 547–553

    Abstract: Human stem cells are born with the creation of life itself and some of them remain throughout life. Therefore, they can be found in adult tissues and used for basic and applied research. Currently, in our country there is a growing interest in the study ... ...

    Title translation Procedure for culture and identification of stem cells from human lipoaspirate for research purposes.
    Abstract Human stem cells are born with the creation of life itself and some of them remain throughout life. Therefore, they can be found in adult tissues and used for basic and applied research. Currently, in our country there is a growing interest in the study and application of stem cells; however, little is known about the identification procedure. For this reason, this study aims to present, from a practical point of view, a procedure for the culture and identification of stem/stromal cells obtained from human lipoaspirate (Adipose Stem Cells), for research purposes. This procedure includes the immunophenotype characterization, cell differentiation potential, gene expression and cell culture quality control; and will serve as support for Peruvian scientific community professionals who wish to develop this line of research.
    MeSH term(s) Adipose Tissue/cytology ; Adult ; Culture Techniques ; Humans ; Research ; Stem Cells
    Language Spanish
    Publishing date 2020-12-08
    Publishing country Peru
    Document type Journal Article
    ZDB-ID 2120092-0
    ISSN 1726-4642 ; 1726-4634
    ISSN (online) 1726-4642
    ISSN 1726-4634
    DOI 10.17843/rpmesp.2020.373.5201
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Cytotoxic activity of the chloroform fraction of Piper aduncum and its effect on the cell cycle in gastric cancer cell lines.

    Mayanga-Herrera, Ana / Tapia-Rojas, Salyoc / Fukusaki-Yoshizawa, Alejandro / Marcelo-Rodríguez, Álvaro / Amiel-Pérez, José

    Revista peruana de medicina experimental y salud publica

    2020  Volume 37, Issue 3, Page(s) 471–477

    Abstract: Objectives: To evaluate the cytotoxic activity of the chloroform fraction of the Piper aduncum methanolic extract (PAMoCl) and its effect on the cell cycle in two gastric cancer cell lines: AGS and KATO III.: Materials and methods: The cytotoxic ... ...

    Title translation Actividad citotóxica de la fracción clorofórmica de Piper aduncum y su efecto en el ciclo celular en líneas celulares de cáncer gástrico.
    Abstract Objectives: To evaluate the cytotoxic activity of the chloroform fraction of the Piper aduncum methanolic extract (PAMoCl) and its effect on the cell cycle in two gastric cancer cell lines: AGS and KATO III.
    Materials and methods: The cytotoxic effect of PAMoCl was evaluated in cell lines AGS and KATO III. The following PAMoCl concentrations were tested, 1.25, 2.5, 5, 10, 20, 40, 80 and 160 μg/mL. Resazurine was used to evaluate cell viability. In the cell cycle assay, the cells were treated with 19.62 μg/mL and 39.23 μg/mL of PAMoCl for AGS as well as 87.49 μg/mL and 160 μg/mL for KATO III. After 24 hours both cell lines were analyzed by flow cytometry.
    Results: PAMoCl showed cytotoxic activity, inhibiting cell growth by 50%. It presented a (IC50) of 39.23 μg/mL and 87.49 μg/mL at 24 hours and a (IC50) of 49.47 μg/mL and 64.68 μg/mL at 48 hours against AGS and KATO III cell lines, respectively. In addition, it was observed that PAMoCl has an effect on the cell cycle, it causes an accumulation of cells in the G2/M phase.
    Conclusions: PAMoCl contains secondary metabolites with cytotoxic activity that have an effect on the G2/M phase of the cell cycle, in two gastric cancer cell lines, both primary and metastatic. The results of this study will allow us to deepen the search for more effective active ingredients found in PAMoCl for eliminating gastric cancer cells, but with less toxicity for healthy cells.
    MeSH term(s) Cell Cycle/drug effects ; Cell Line, Tumor/cytology ; Cell Line, Tumor/drug effects ; Chloroform/pharmacology ; Cytotoxins ; Humans ; Piper ; Stomach Neoplasms/drug therapy
    Chemical Substances Cytotoxins ; Chloroform (7V31YC746X)
    Language English
    Publishing date 2020-12-07
    Publishing country Peru
    Document type Journal Article
    ZDB-ID 2120092-0
    ISSN 1726-4642 ; 1726-4642
    ISSN (online) 1726-4642
    ISSN 1726-4642
    DOI 10.17843/rpmesp.2020.373.5157
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Cytokine Profiles Associated With Worse Prognosis in a Hospitalized Peruvian COVID-19 Cohort.

    Pons, Maria J / Ymaña, Barbara / Mayanga-Herrera, Ana / Sáenz, Yolanda / Alvarez-Erviti, Lydia / Tapia-Rojas, Salyoc / Gamarra, Roxana / Blanco, Amanda B / Moncunill, Gemma / Ugarte-Gil, Manuel F

    Frontiers in immunology

    2021  Volume 12, Page(s) 700921

    Abstract: Cytokines, chemokines and growth factors present different expression profiles related to the prognosis of COVID-19. We analyzed clinical parameters and assessed the expression of these biomarkers in patients with different disease severity in a ... ...

    Abstract Cytokines, chemokines and growth factors present different expression profiles related to the prognosis of COVID-19. We analyzed clinical parameters and assessed the expression of these biomarkers in patients with different disease severity in a hospitalized Peruvian cohort to determine those associated with worse prognosis. We measured anti-spike IgG antibodies by ELISA and 30 cytokines by quantitative suspension array technology in 123 sera samples. We analyzed differences between patients with moderate, severe and fatal COVID-19 by logistic regression at baseline and in longitudinal samples. Significant differences were found among the clinical parameters: hemoglobin, neutrophils, lymphocytes and C-reactive protein (CRP), creatinine and D-dimer levels. Higher anti-spike IgG antibody concentrations were associated to fatal patient outcomes. At hospitalization, IL-10, IL-6, MIP-1α, GM-CSF, MCP-1, IL-15, IL-5, IL1RA, TNFα and IL-8 levels were already increased in fatal patients´ group. Meanwhile, multivariable analysis revealed that increased GM-CSF, MCP-1, IL-15, and IL-8 values were associated with fatal outcomes. Moreover, longitudinal analysis identified IL-6 and MCP-1 as the main risk factors related to mortality in hospitalized COVID-19 patients. In this Peruvian cohort we identified and validated biomarkers related to COVID-19 outcomes. Further studies are needed to identify novel criteria for stratification of SARS-CoV-2 infected patients at hospital entry.
    Background: In the most severe forms of SARS-CoV-2 infection, large numbers of innate and adaptive immune cells become activated and begin to produce pro-inflammatory cytokines, establishing an exacerbated feedback loop of inflammation.
    Methods: A total of 55 patients with laboratory-confirmed COVID-19 admitted to the
    Results: The frequency of obesity differed among the 3 groups, being most frequent in patients who died. There were also significant differences in clinical parameters: hemoglobin, segmented neutrophils, lymphocytes,C-reactive protein, creatinine and D-dimer levels. Greater anti-spike IgG antibody concentrations were associated to fatal outcomes. In univariate analyses, higher baseline concentrations of IL-6, MIP-1α, GM-CSF, MCP-1, IL-15, IL-5, IL1RA, TNFα, IL-8 and IL-12p70 correlated with severity, while multivariable analysis showed that increased concentrations in 4 biomarkers (GM-CSF, MCP-1, IL-15, IL-8) were associated with fatal outcomes. Longitudinal analysis showed IL-6 (hazard ratio [HR] 6.81, 95% confidence interval [CI] 1.6-28.7) and MCP-1 (HR 4.61, 95%CI 1.1-19.1) to be related to mortality in hospitalized COVID-19 patients.
    Conclusions: Cytokine, chemokine and growth factor profiles were identified and validated related to severity and outcomes of COVID-19. Our findings may be useful to identify novel criteria for COVID-19 patient stratification at hospital entry.
    MeSH term(s) Antibodies, Viral/blood ; Antibodies, Viral/immunology ; Biomarkers/blood ; COVID-19/blood ; COVID-19/immunology ; COVID-19/mortality ; Comorbidity ; Cytokines/blood ; Enzyme-Linked Immunosorbent Assay ; Female ; Hospitalization ; Humans ; Immunoglobulin G/blood ; Male ; Middle Aged ; Obesity/epidemiology ; Peru/epidemiology ; Prognosis ; SARS-CoV-2/immunology ; Severity of Illness Index ; Spike Glycoprotein, Coronavirus/immunology
    Chemical Substances Antibodies, Viral ; Biomarkers ; Cytokines ; Immunoglobulin G ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
    Language English
    Publishing date 2021-09-01
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2021.700921
    Database MEDical Literature Analysis and Retrieval System OnLINE

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