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  1. AU="Tashiro, Ryo"
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  1. Article ; Online: Altering the Phosphorylation Position of Pyrophosphate-Dependent

    Tashiro, Ryo / Sato, Takaaki / Atomi, Haruyuki / Miki, Kunio / Fujihashi, Masahiro

    ACS chemical biology

    2021  Volume 16, Issue 5, Page(s) 794–799

    Abstract: Most kinases utilize ATP as a phosphate donor and phosphorylate a wide range of phosphate acceptors. An alternative phosphate donor is inorganic pyrophosphate (PPi), which costs only 1/1000 of ATP. To develop a method to engineer PPi-dependent kinases, ... ...

    Abstract Most kinases utilize ATP as a phosphate donor and phosphorylate a wide range of phosphate acceptors. An alternative phosphate donor is inorganic pyrophosphate (PPi), which costs only 1/1000 of ATP. To develop a method to engineer PPi-dependent kinases, we herein aimed to alter the product of PPi-dependent
    MeSH term(s) Catalytic Domain ; Crystallization ; Diphosphates/chemistry ; Inositol Phosphates/chemistry ; Kinetics ; Magnetic Resonance Spectroscopy ; Mutant Proteins/chemistry ; Mutation ; Phosphoric Monoester Hydrolases/chemistry ; Phosphorylation ; Protein Conformation ; Tandem Mass Spectrometry ; Thermotoga maritima/enzymology
    Chemical Substances Diphosphates ; Inositol Phosphates ; Mutant Proteins ; inositol 1-phosphate (15421-51-9) ; inositol 3-phosphate (2831-74-5) ; diphosphoric acid (4E862E7GRQ) ; Phosphoric Monoester Hydrolases (EC 3.1.3.2) ; myo-inositol-1 (or 4)-monophosphatase (EC 3.1.3.25)
    Language English
    Publishing date 2021-04-20
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1554-8937
    ISSN (online) 1554-8937
    DOI 10.1021/acschembio.0c00733
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Secundum atrial septal defect resulting in hypoxaemia.

    Iio, Chiharuko / Inoue, Katsuji / Tashiro, Ryo / Higaki, Takashi / Higaki, Jitsuo

    European heart journal cardiovascular Imaging

    2014  Volume 15, Issue 1, Page(s) 103

    MeSH term(s) Diagnosis, Differential ; Echocardiography/methods ; Echocardiography, Three-Dimensional ; Echocardiography, Transesophageal ; Female ; Heart Septal Defects, Atrial/diagnostic imaging ; Heart Septal Defects, Atrial/surgery ; Humans ; Middle Aged ; Septal Occluder Device
    Language English
    Publishing date 2014-01
    Publishing country England
    Document type Case Reports ; Journal Article ; Video-Audio Media
    ZDB-ID 2638345-7
    ISSN 2047-2412 ; 2047-2404
    ISSN (online) 2047-2412
    ISSN 2047-2404
    DOI 10.1093/ehjci/jet106
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5614: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article: Nonocclusive Mesenteric Ischemia Rescued by Immediate Surgical Exploration in a Boy with Severe Neurodevelopmental Disability.

    Mizumoto, Manami / Ochi, Fumihiro / Jogamoto, Toshihiro / Okamoto, Kentaro / Fukuda, Mitsumasa / Yamauchi, Toshifumi / Miyata, Toyohisa / Tashiro, Ryo / Eguchi, Mariko / Kitazawa, Riko / Ishii, Eiichi

    Case reports in pediatrics

    2019  Volume 2019, Page(s) 5354074

    Abstract: Background: Nonocclusive mesenteric ischemia (NOMI) defines acute mesenteric ischemia without occlusion of the mesenteric arteries. The most common cause of NOMI is vasoconstriction or vasospasm of a mesenteric artery. NOMI generally affects patients > ... ...

    Abstract Background: Nonocclusive mesenteric ischemia (NOMI) defines acute mesenteric ischemia without occlusion of the mesenteric arteries. The most common cause of NOMI is vasoconstriction or vasospasm of a mesenteric artery. NOMI generally affects patients >50 years of age, and few cases have been reported in children.
    Case presentation: A 15-year-old boy with severe neurodevelopmental disability developed sudden-onset fever, abdominal distention, and dyspnea. Laboratory and radiological findings indicated acute intestinal obstruction and prerenal failure. He developed transient cardiopulmonary arrest and hypovolemic shock. Emergent laparotomy was performed, which revealed segmentally necrotic intestine from the jejunum to the ascending colon with pulsation of peripheral intestinal arteries, leading to a diagnosis of NOMI. The necrotic intestine was resected, and stomas were created. He was discharged on postoperative day 334 with short bowel syndrome as a complication.
    Conclusions: NOMI should be considered a differential diagnosis for intestinal symptoms with severe general conditions in both adults and children with underlying disease. Immediate surgical exploration is essential with NOMI to save a patient's life.
    Language English
    Publishing date 2019-02-19
    Publishing country United States
    Document type Case Reports
    ZDB-ID 2659094-3
    ISSN 2090-6811 ; 2090-6803
    ISSN (online) 2090-6811
    ISSN 2090-6803
    DOI 10.1155/2019/5354074
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Intracellular trafficking of Clostridium botulinum C2 toxin.

    Nagahama, Masahiro / Takahashi, Chihiro / Aoyanagi, Kouhei / Tashiro, Ryo / Kobayashi, Keiko / Sakaguchi, Yoshihiko / Ishidoh, Kazumi / Sakurai, Jun

    Toxicon : official journal of the International Society on Toxinology

    2014  Volume 82, Page(s) 76–82

    Abstract: Clostridium botulinum C2 toxin is a binary toxin composed of an enzymatic component (C2I) and binding component (C2II). The activated binding component (C2IIa) forms heptamers and the oligomer with C2I is taken up by receptor-mediated endocytosis. We ... ...

    Abstract Clostridium botulinum C2 toxin is a binary toxin composed of an enzymatic component (C2I) and binding component (C2II). The activated binding component (C2IIa) forms heptamers and the oligomer with C2I is taken up by receptor-mediated endocytosis. We investigated the intracellular trafficking of C2 toxin. When MDCK cells were incubated with C2I and C2IIa at 37 °C, C2I colocalized with C2IIa in cytoplasmic vesicles at 5 min, and C2I then disappeared (15 min incubation and later), and C2IIa was observed in the vesicles. Internalized C2I and C2IIa were transported to early endosomes. Some of both components were returned to the plasma membrane through recycling endosomes, whereas the rest of C2IIa was transported to late endosomes and lysosomes for degradation. Bafilomycin A1, an endosomal acidification inhibitor, caused the accumulation of C2IIa in endosomes, and both nocodazole and colchicine, microtubule-disrupting agents, restricted C2IIa's movement in the cytosol. These results indicated that an internalized C2I and C2IIa complex was delivered to early endosomes, and that subsequent delivery of C2I to the cytoplasm occurred in early endosomes. C2IIa was either sent back to the plasma membranes through recycling endosomes or transported to late endosomes and lysosomes for degradation.
    MeSH term(s) Animals ; Botulinum Toxins/antagonists & inhibitors ; Botulinum Toxins/metabolism ; Botulinum Toxins/toxicity ; Cell Line ; Cell Membrane/metabolism ; Cell Survival/drug effects ; Cytoplasm/metabolism ; Dogs ; Endocytosis/drug effects ; Endosomes/metabolism ; Macrolides/pharmacology ; Recombinant Proteins/metabolism
    Chemical Substances Macrolides ; Recombinant Proteins ; bafilomycin A1 (88899-55-2) ; Botulinum Toxins (EC 3.4.24.69) ; botulinum toxin type C (FPM7829VMX)
    Language English
    Publishing date 2014-05
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 204479-1
    ISSN 1879-3150 ; 0041-0101
    ISSN (online) 1879-3150
    ISSN 0041-0101
    DOI 10.1016/j.toxicon.2014.02.009
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 501: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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  5. Article ; Online: Two-Phase Contrast Injection Protocol for Pediatric Cardiac Computed Tomography in Children with Congenital Heart Disease.

    Fukuyama, Naoki / Kurata, Akira / Kawaguchi, Naoto / Tashiro, Ryo / Higaki, Takashi / Yokoi, Takahiro / Tanabe, Yuki / Nishiyama, Hikaru / Itoh, Toshihide / Kido, Teruhito / Miyagawa, Masao / Mochizuki, Teruhito

    Pediatric cardiology

    2017  Volume 39, Issue 3, Page(s) 518–525

    Abstract: To assess a two-phase contrast injection protocol for contrast enhancement during cardiac computed tomography (CT) in children with congenital heart disease. Forty-three children (20 boys, 23 girls) of median age 13 months (range 3 days-8.3 years) and ... ...

    Abstract To assess a two-phase contrast injection protocol for contrast enhancement during cardiac computed tomography (CT) in children with congenital heart disease. Forty-three children (20 boys, 23 girls) of median age 13 months (range 3 days-8.3 years) and weighing ≤ 20 kg who underwent cardiac CT using a two-phase contrast injection protocol at our institution were retrospectively identified. High-pitch spiral third-generation dual-source cardiac CT (tube voltage 70 kV) was performed with a fixed delay of 60 s after contrast injection in the order of 10 mgI/kg/s (30 s), 15 mgI/kg/s (20 s), and a saline chaser (10 s). Attenuation in the inferior vena cava (IVC), superior vena cava (SVC), right atrium (RA), right ventricle (RV), pulmonary artery (PA), left atrium (LA), left ventricle (LV), and descending aorta (AO) was compared using the Steel-Dwass and Fisher's exact tests. The median (interquartile range) attenuation in the IVC, SVC, RA, RV, PA, LA, LV, and AO was 285 (264-347) Hounsfield units (HU), 416 (370-445) HU, 368 (320-388) HU, 373 (322-417) HU, 397 (330-432) HU, 425 (373-469) HU, 435 (385-468) HU, and 437 (392-491) HU, respectively (p < 0.05, IVC vs. the other anatomic sites). There was no significant difference in diagnostic success rate for attenuation > 250 HU between the IVC (41 children, 95.3%) and the other sites (43 children, 100%). A two-phase contrast injection protocol is useful for effective contrast enhancement in pediatric cardiac CT.
    MeSH term(s) Aorta, Thoracic/diagnostic imaging ; Child ; Child, Preschool ; Contrast Media/administration & dosage ; Female ; Heart Atria/diagnostic imaging ; Heart Defects, Congenital/diagnostic imaging ; Heart Ventricles/diagnostic imaging ; Humans ; Infant ; Infant, Newborn ; Injections, Intravenous/methods ; Male ; Pulmonary Artery/diagnostic imaging ; Retrospective Studies ; Tomography, X-Ray Computed/methods ; Vena Cava, Inferior/diagnostic imaging ; Vena Cava, Superior/diagnostic imaging
    Chemical Substances Contrast Media
    Language English
    Publishing date 2017-12-06
    Publishing country United States
    Document type Journal Article
    ZDB-ID 800857-7
    ISSN 1432-1971 ; 0172-0643
    ISSN (online) 1432-1971
    ISSN 0172-0643
    DOI 10.1007/s00246-017-1782-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1528: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  6. Article ; Online: Intracellular trafficking of Clostridium perfringens iota-toxin b.

    Nagahama, Masahiro / Umezaki, Mariko / Tashiro, Ryo / Oda, Masataka / Kobayashi, Keiko / Shibutani, Masahiro / Takagishi, Teruhisa / Ishidoh, Kazumi / Fukuda, Mitsunori / Sakurai, Jun

    Infection and immunity

    2012  Volume 80, Issue 10, Page(s) 3410–3416

    Abstract: Clostridium perfringens iota-toxin is composed of an enzymatic component (Ia) and a binding component (Ib). Ib binds to a cell surface receptor, undergoes oligomerization in lipid rafts, and binds Ia. The resulting complex is then endocytosed. Here, we ... ...

    Abstract Clostridium perfringens iota-toxin is composed of an enzymatic component (Ia) and a binding component (Ib). Ib binds to a cell surface receptor, undergoes oligomerization in lipid rafts, and binds Ia. The resulting complex is then endocytosed. Here, we show the intracellular trafficking of iota-toxin. After the binding of the Ib monomer with cells at 4°C, oligomers of Ib formed at 37°C and later disappeared. Immunofluorescence staining of Ib revealed that the internalized Ib was transported to early endosomes. Some Ib was returned to the plasma membrane through recycling endosomes, whereas the rest was transported to late endosomes and lysosomes for degradation. Degraded Ib was delivered to the plasma membrane by an increase in the intracellular Ca(2+) concentration caused by Ib. Bafilomycin A1, an endosomal acidification inhibitor, caused the accumulation of Ib in endosomes, and both nocodazole and colchicine, microtubule-disrupting agents, restricted Ib's movement in the cytosol. These results indicated that an internalized Ia and Ib complex was delivered to early endosomes and that subsequent delivery of Ia to the cytoplasm occurs mainly in early endosomes. Ib was either sent back to the plasma membranes through recycling endosomes or transported to late endosomes and lysosomes for degradation. Degraded Ib was transported to plasma membranes.
    MeSH term(s) ADP Ribose Transferases/classification ; ADP Ribose Transferases/genetics ; ADP Ribose Transferases/metabolism ; Animals ; Bacterial Toxins/classification ; Bacterial Toxins/genetics ; Bacterial Toxins/metabolism ; Calcium/metabolism ; Cell Line ; Cell Membrane/metabolism ; Cell Shape/drug effects ; Clostridium perfringens/genetics ; Clostridium perfringens/metabolism ; Dogs ; Endosomes/metabolism ; Gene Expression Regulation, Bacterial/physiology ; Immunoblotting ; Macrolides ; Protein Binding ; Protein Transport/physiology
    Chemical Substances Bacterial Toxins ; Macrolides ; iota toxin, Clostridium perfringens ; bafilomycin A1 (88899-55-2) ; ADP Ribose Transferases (EC 2.4.2.-) ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2012-07-23
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 218698-6
    ISSN 1098-5522 ; 0019-9567
    ISSN (online) 1098-5522
    ISSN 0019-9567
    DOI 10.1128/IAI.00483-12
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 684: Show issues Location:
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