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  1. Article ; Online: An Ionizable Lipid Material with a Vitamin E Scaffold as an mRNA Vaccine Platform for Efficient Cytotoxic T Cell Responses.

    Oyama, Ryotaro / Ishigame, Harumichi / Tanaka, Hiroki / Tateshita, Naho / Itazawa, Moeko / Imai, Ryosuke / Nishiumi, Naomasa / Kishikawa, Jun-Ichi / Kato, Takayuki / Anindita, Jessica / Nishikawa, Yoshifumi / Maeki, Masatoshi / Tokeshi, Manabu / Tange, Kota / Nakai, Yuta / Sakurai, Yu / Okada, Takaharu / Akita, Hidetaka

    ACS nano

    2023  Volume 17, Issue 19, Page(s) 18758–18774

    Abstract: RNA vaccines based on lipid nanoparticles (LNPs) ... ...

    Abstract RNA vaccines based on lipid nanoparticles (LNPs) with
    MeSH term(s) Animals ; Mice ; T-Lymphocytes, Cytotoxic ; CD8-Positive T-Lymphocytes ; Vitamin E/pharmacology ; Vaccines, Synthetic ; mRNA Vaccines ; Antigens ; Ovalbumin ; Nanoparticles ; RNA, Messenger/genetics ; Lipids/pharmacology ; Mice, Inbred C57BL ; Dendritic Cells
    Chemical Substances Vitamin E (1406-18-4) ; Vaccines, Synthetic ; mRNA Vaccines ; Antigens ; Ovalbumin (9006-59-1) ; RNA, Messenger ; Lipids
    Language English
    Publishing date 2023-09-26
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1936-086X
    ISSN (online) 1936-086X
    DOI 10.1021/acsnano.3c02251
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Modifying Antigen-Encapsulating Liposomes with KALA Facilitates MHC Class I Antigen Presentation and Enhances Anti-tumor Effects.

    Miura, Naoya / Akita, Hidetaka / Tateshita, Naho / Nakamura, Takashi / Harashima, Hideyoshi

    Molecular therapy : the journal of the American Society of Gene Therapy

    2017  Volume 25, Issue 4, Page(s) 1003–1013

    Abstract: For a successful anti-cancer vaccine, antigen presentation on the major histocompatibility complex (MHC) class I is a requirement. To accomplish this, an antigen must be delivered to the cytoplasm by overcoming the endosome/lysosome. We previously ... ...

    Abstract For a successful anti-cancer vaccine, antigen presentation on the major histocompatibility complex (MHC) class I is a requirement. To accomplish this, an antigen must be delivered to the cytoplasm by overcoming the endosome/lysosome. We previously reported that a lipid nanoparticle modified with a KALA peptide (WEAKLAKALAKALAKHLAKALAKALKA), an α-helical cationic peptide, permits the encapsulated pDNA to be efficiently delivered to the cytoplasm in bone marrow-derived dendritic cells (BMDCs). Herein, we report on the use of KALA-modified liposomes as an antigen carrier, in an attempt to induce potent antigen-specific cellular immunity. The subcutaneous injection of KALA-modified ovalbumin (OVA)-encapsulating liposomes (KALA-OVA-LPs) elicited a much more potent OVA-specific cytotoxic T lymphocyte activity and anti-tumor effect in comparison with particles that were modified with octa-arginine (R8), a cell-penetrating peptide (R8-OVA-LPs). In addition, the numbers of OVA-specific CD8
    MeSH term(s) Animals ; Antigen Presentation/immunology ; Antigens/chemistry ; Antigens/immunology ; Cancer Vaccines/immunology ; Cell Line, Tumor ; Cytokines/metabolism ; DNA-Binding Proteins/chemistry ; Dendritic Cells/immunology ; Disease Models, Animal ; Female ; Histocompatibility Antigens Class I/chemistry ; Histocompatibility Antigens Class I/immunology ; Immunization ; Liposomes ; Mice ; Neoplasms/immunology ; Neoplasms/therapy ; Ovalbumin/immunology ; Peptides/chemistry ; T-Lymphocytes, Cytotoxic/immunology
    Chemical Substances Antigens ; Cancer Vaccines ; Cytokines ; DNA-Binding Proteins ; Histocompatibility Antigens Class I ; KALA amphipathic peptide ; Liposomes ; Peptides ; Ovalbumin (9006-59-1)
    Language English
    Publishing date 2017-04-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2010592-7
    ISSN 1525-0024 ; 1525-0016
    ISSN (online) 1525-0024
    ISSN 1525-0016
    DOI 10.1016/j.ymthe.2017.01.020
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5640: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article: Development of a lipoplex-type mRNA carrier composed of an ionizable lipid with a vitamin E scaffold and the KALA peptide for use as an ex vivo dendritic cell-based cancer vaccine

    Tateshita, Naho / Miura, Naoya / Tanaka, Hiroki / Masuda, Takeshi / Ohtsuki, Sumio / Tange, Kota / Nakai, Yuta / Yoshioka, Hiroki / Akita, Hidetaka

    Journal of controlled release. 2019 Sept. 28, v. 310

    2019  

    Abstract: A dendritic cells (DCs)-based vaccine (DC-vaccine) system is an attractive technology for eliciting antigen-specific immune responses that can protect subjects from infectious diseases and for curing various types of cancers. For the insertion of a ... ...

    Abstract A dendritic cells (DCs)-based vaccine (DC-vaccine) system is an attractive technology for eliciting antigen-specific immune responses that can protect subjects from infectious diseases and for curing various types of cancers. For the insertion of a foreign antigen to DCs, the transfection of an antigen-coding mRNA to the cells is a promising approach. In order to introduce an antigen, a carrier for mRNA transfection is required, since the mRNA molecule per se is unstable in serum-containing medium. We previously reported on an ionizable lipid-like material with vitamin E-scaffolds (ssPalmE) as a material for a lipid nanoparticle (LNP)-based carrier for nucleic acids. In the present study, we report on the development of a lipoplex-type mRNA carrier for use as a DC-vaccine by using a combination of an ssPalmE-LNP and an α-helical cationic peptide “KALA” (ssPalmE-KALA). The transfection of mRNAs complexed with the ssPalmE-KALA achieved a significantly higher protein expression and the production of proinflammatory cytokines from murine bone marrow derived DCs (BMDCs) in comparison with a lipoplex that was prepared with an ssPalm with fatty acid-scaffolds (myristic acid; ssPalmM-KALA). A cellular uptake process and a pH-responsive membrane-destabilization activity cannot explain the preferred protein expression and immune-stimulation caused by the ssPalmE-KALA. Proteomic analyses suggest that transfection with the ssPalmM-KALA stimulates a down-regulatory pathway of translation, while the transfection with the ssPalmE-KALA does not stimulate it. In the vaccination with the BMDCs that were preliminarily transfected with an ovalbumin (OVA)-encoding mRNA elicited the induction OVA specific cytotoxic T-lymphocyte activity in vivo. In parallel, the vaccination induced significant prophylactic anti-tumor effects against a model tumor that stably expressed the OVA protein. Based on the above findings, the ssPalmE-KALA appears to be a potent ex vivo DCs-based RNA vaccine platform.
    Keywords T-lymphocytes ; antigens ; antineoplastic activity ; bone marrow ; cancer vaccines ; cytokines ; cytotoxicity ; dendritic cells ; gene expression regulation ; immune response ; infectious diseases ; messenger RNA ; mice ; models ; myristic acid ; nanoparticles ; neoplasms ; ovalbumin ; protein synthesis ; proteomics ; transfection ; translation (genetics) ; vaccination ; vitamin E
    Language English
    Dates of publication 2019-0928
    Size p. 36-46.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 632533-6
    ISSN 1873-4995 ; 0168-3659
    ISSN (online) 1873-4995
    ISSN 0168-3659
    DOI 10.1016/j.jconrel.2019.08.002
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2055: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Development of a lipoplex-type mRNA carrier composed of an ionizable lipid with a vitamin E scaffold and the KALA peptide for use as an ex vivo dendritic cell-based cancer vaccine.

    Tateshita, Naho / Miura, Naoya / Tanaka, Hiroki / Masuda, Takeshi / Ohtsuki, Sumio / Tange, Kota / Nakai, Yuta / Yoshioka, Hiroki / Akita, Hidetaka

    Journal of controlled release : official journal of the Controlled Release Society

    2019  Volume 310, Page(s) 36–46

    Abstract: A dendritic cells (DCs)-based vaccine (DC-vaccine) system is an attractive technology for eliciting antigen-specific immune responses that can protect subjects from infectious diseases and for curing various types of cancers. For the insertion of a ... ...

    Abstract A dendritic cells (DCs)-based vaccine (DC-vaccine) system is an attractive technology for eliciting antigen-specific immune responses that can protect subjects from infectious diseases and for curing various types of cancers. For the insertion of a foreign antigen to DCs, the transfection of an antigen-coding mRNA to the cells is a promising approach. In order to introduce an antigen, a carrier for mRNA transfection is required, since the mRNA molecule per se is unstable in serum-containing medium. We previously reported on an ionizable lipid-like material with vitamin E-scaffolds (ssPalmE) as a material for a lipid nanoparticle (LNP)-based carrier for nucleic acids. In the present study, we report on the development of a lipoplex-type mRNA carrier for use as a DC-vaccine by using a combination of an ssPalmE-LNP and an α-helical cationic peptide "KALA" (ssPalmE-KALA). The transfection of mRNAs complexed with the ssPalmE-KALA achieved a significantly higher protein expression and the production of proinflammatory cytokines from murine bone marrow derived DCs (BMDCs) in comparison with a lipoplex that was prepared with an ssPalm with fatty acid-scaffolds (myristic acid; ssPalmM-KALA). A cellular uptake process and a pH-responsive membrane-destabilization activity cannot explain the preferred protein expression and immune-stimulation caused by the ssPalmE-KALA. Proteomic analyses suggest that transfection with the ssPalmM-KALA stimulates a down-regulatory pathway of translation, while the transfection with the ssPalmE-KALA does not stimulate it. In the vaccination with the BMDCs that were preliminarily transfected with an ovalbumin (OVA)-encoding mRNA elicited the induction OVA specific cytotoxic T-lymphocyte activity in vivo. In parallel, the vaccination induced significant prophylactic anti-tumor effects against a model tumor that stably expressed the OVA protein. Based on the above findings, the ssPalmE-KALA appears to be a potent ex vivo DCs-based RNA vaccine platform.
    MeSH term(s) Animals ; Cancer Vaccines/administration & dosage ; Cancer Vaccines/genetics ; Cancer Vaccines/immunology ; Cell-Penetrating Peptides/chemistry ; Cytokines/biosynthesis ; Dendritic Cells/immunology ; Drug Carriers/chemistry ; Gene Expression ; Lipids/chemistry ; Liposomes ; Mice, Inbred C57BL ; Ovalbumin/genetics ; Ovalbumin/immunology ; RNA, Messenger/administration & dosage ; RNA, Messenger/genetics ; RNA, Messenger/immunology ; T-Lymphocytes, Cytotoxic/immunology ; Vitamin E/chemistry
    Chemical Substances Cancer Vaccines ; Cell-Penetrating Peptides ; Cytokines ; Drug Carriers ; Lipids ; Liposomes ; RNA, Messenger ; Vitamin E (1406-18-4) ; Ovalbumin (9006-59-1)
    Language English
    Publishing date 2019-08-03
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 632533-6
    ISSN 1873-4995 ; 0168-3659
    ISSN (online) 1873-4995
    ISSN 0168-3659
    DOI 10.1016/j.jconrel.2019.08.002
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 2055: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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