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Article ; Online: Degradation modeling of water environmental DNA

Tatsuya Saito / Hideyuki Doi

Environmental DNA, Vol 3, Iss 4, Pp 850-

Experiments on multiple DNA sources in pond and seawater

2021 Volume 860

Abstract: Abstract Environmental DNA (eDNA) analysis methods have been developed to detect organism distribution and abundance/biomass in various environments. eDNA degradation is critical for eDNA evaluation. However, the dynamics and mechanisms of eDNA ... ...

Abstract	Abstract Environmental DNA (eDNA) analysis methods have been developed to detect organism distribution and abundance/biomass in various environments. eDNA degradation is critical for eDNA evaluation. However, the dynamics and mechanisms of eDNA degradation are largely unknown, especially when considering different eDNA sources, for example, cells and fragmental DNA. We experimentally evaluated the degradation rates of eDNA derived from multiple sources, including fragmental DNA (internal PCR control [IPC]), free cells (from Oncorhynchus kisutch), and resident species. We conducted the experiment with pond and seawater to evaluate the differences between freshwater and marine habitats. We quantified the eDNA copies of free cells, fragmental DNA, and resident species (Cyprinus carpio in the pond and Trachurus japonicus in the sea). We found that eDNA derived from both cells and fragmental DNA decreased exponentially in both the sea and pond samples. The degradation of eDNA from resident species showed similar behavior to the cell‐derived eDNA. We evaluated three degradation models with different assumptions and degradation steps and found that a simple exponential model was effective in most cases. Our findings on cell‐ and fragmental DNA‐derived eDNA provide fundamental information about the eDNA degradation process and can be applied to quantify eDNA behavior in natural environments.
Keywords	decay rate ; degradation modeling ; eDNA ; quantitative real‐time PCR ; Environmental sciences ; GE1-350 ; Microbial ecology ; QR100-130
Subject code	612
Language	English
Publishing date	2021-07-01T00:00:00Z
Publisher	Wiley
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: A Model and Simulation of the Influence of Temperature and Amplicon Length on Environmental DNA Degradation Rates

Tatsuya Saito / Hideyuki Doi

Frontiers in Ecology and Evolution, Vol

A Meta-Analysis Approach

2021 Volume 9

Abstract: Environmental DNA (eDNA) analysis can detect aquatic organisms, including rare and endangered species, in a variety of habitats. Degradation can influence eDNA persistence, impacting eDNA-based species distribution and occurrence results. Previous ... ...

Abstract	Environmental DNA (eDNA) analysis can detect aquatic organisms, including rare and endangered species, in a variety of habitats. Degradation can influence eDNA persistence, impacting eDNA-based species distribution and occurrence results. Previous studies have investigated degradation rates and associated contributing factors. It is important to integrate data from across these studies to better understand and synthesize eDNA degradation in various environments. We complied the eDNA degradation rates and related factors, especially water temperature and amplicon lengths of the measured DNA from 28 studies, and subjected the data to a meta-analysis. In agreement with previous studies, our results suggest that water temperature and amplicon length are significantly related to the eDNA degradation rate. From the 95% quantile model simulation, we predicted the maximum eDNA degradation rate in various combinations of water temperature and amplicon length. Predicting eDNA degradation could be important for evaluating species distribution and inducing innovation (e.g., sampling, extraction, and analysis) of eDNA methods, especially for rare and endangered species with small population size.
Keywords	environmental DNA ; polymerase chain reaction ; degradation rate ; quantile model ; meta-analysis ; Evolution ; QH359-425 ; Ecology ; QH540-549.5
Language	English
Publishing date	2021-03-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Suprabasin-derived bioactive peptides identified by plasma peptidomics

Tomomi Taguchi / Yoshio Kodera / Kazuhito Oba / Tatsuya Saito / Yuzuru Nakagawa / Yusuke Kawashima / Masayoshi Shichiri

Scientific Reports, Vol 11, Iss 1, Pp 1-

2021 Volume 17

Abstract: Abstract Identification of low-abundance, low-molecular-weight native peptides using non-tryptic plasma has long remained an unmet challenge, leaving potential bioactive/biomarker peptides undiscovered. We have succeeded in efficiently removing high- ... ...

Abstract	Abstract Identification of low-abundance, low-molecular-weight native peptides using non-tryptic plasma has long remained an unmet challenge, leaving potential bioactive/biomarker peptides undiscovered. We have succeeded in efficiently removing high-abundance plasma proteins to enrich and comprehensively identify low-molecular-weight native peptides using mass spectrometry. Native peptide sequences were chemically synthesized and subsequent functional analyses resulted in the discovery of three novel bioactive polypeptides derived from an epidermal differentiation marker protein, suprabasin. SBSN_HUMAN[279–295] potently suppressed food/water intake and induced locomotor activity when injected intraperitoneally, while SBSN_HUMAN[225–237] and SBSN_HUMAN[243–259] stimulated the expression of proinflammatory cytokines via activation of NF-κB signaling in vascular cells. SBSN_HUMAN[225–237] and SBSN_HUMAN[279–295] immunoreactivities were present in almost all human organs analyzed, while immunoreactive SBSN_HUMAN[243–259] was abundant in the liver and pancreas. Human macrophages expressed the three suprabasin-derived peptides. This study illustrates a new approach for discovering unknown bioactive peptides in plasma via the generation of peptide libraries using a novel peptidomic strategy.
Keywords	Medicine ; R ; Science ; Q
Subject code	610
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Proteomic analysis of AQP11-null kidney

Tatsuya Saito / Yasuko Tanaka / Yoshiyuki Morishita / Kenichi Ishibashi

Biochemistry and Biophysics Reports, Vol 13, Iss , Pp 17-

Proximal tubular type polycystic kidney disease

2018 Volume 21

Abstract: Autosomal Dominant Polycystic Kidney Disease (ADPKD) is caused by the mutation of polycystins (PC-1 or PC-2), in which cysts start from the collecting duct to extend to all nephron segments with eventual end stage renal failure. The cyst development is ... ...

Abstract	Autosomal Dominant Polycystic Kidney Disease (ADPKD) is caused by the mutation of polycystins (PC-1 or PC-2), in which cysts start from the collecting duct to extend to all nephron segments with eventual end stage renal failure. The cyst development is attenuated by a vasopressin V2 receptor antagonist tolvaptan which, however, will not affect proximal tubule cysts devoid of V2 receptor. Aquaporin-11 (AQP11) is expressed selectively in the proximal tubule of the kidney and AQP11-null kidneys have a disruptive PC-1 trafficking to the plasma membrane to develop polycystic kidneys. Here, we analyzed AQP11-null kidneys at the beginning of cyst formation by quantitative proteomic analysis using Tandem Mass Tag (TMT). Among ~ 1200 identified proteins, 124 proteins were differently expressed by > 1.5 or < 0.8 fold change. A pancreatic stone inhibitor or a growth factor, lithostathine-1 (Reg1) was most enhanced by 5 folds which was confirmed by western blot, while mitochondria-related proteins were downregulated. The identified proteins will be new target molecules for the treatment of proximal tubular cysts and helpful to explore the functional roles of AQP11 in the kidney. Keywords: ADPKD, Proximal tubule, AQP11, Proteome, Reg1
Keywords	Biology (General) ; QH301-705.5 ; Biochemistry ; QD415-436
Subject code	616
Language	English
Publishing date	2018-03-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Case Report

Mariko Nakamura / Takashi Naito / Tatsuya Saito / Akari Takahashi / Koji Muraishi / Noriko Hakamada / Mana Otomo / Satoshi Iizuka / Daisuke Nakamura / Hideyuki Takahashi

Frontiers in Sports and Active Living, Vol

Countermeasures Against Heat and Coronavirus for Japanese Athletes at the Tokyo 2020 Olympics and Paralympic Games

2022 Volume 4

Abstract: The Tokyo 2020 Olympics and Paralympic Games were held in the hottest environment in the history of the games. Additionally, the worldwide coronavirus disease 2019 (COVID-19) pandemic necessitated daily polymerase chain reaction (PCR) testing during the ... ...

Abstract	The Tokyo 2020 Olympics and Paralympic Games were held in the hottest environment in the history of the games. Additionally, the worldwide coronavirus disease 2019 (COVID-19) pandemic necessitated daily polymerase chain reaction (PCR) testing during the games, wearing a mask became mandatory publicly, and it was an unheard and unique Olympic with no spectators. Heat acclimation, hydration, and body cooling are essential for safe and high-performance activities in hot environments. In 2015, the Japan Institute of Sports Sciences launched the “Heat Countermeasure Project” to conduct experiments and practical research on heat countermeasures and investigate issues related to heat countermeasures in each athletic event. The results obtained were proposed to various Japan national sports teams, and support for heat countermeasures for the Tokyo 2020 games was promoted in consultation with national federations. Furthermore, due to the COVID-19 pandemic, infectious disease countermeasures for the Tokyo 2020 Games during support were a must. Moreover, athletes, coaches, and team staff could not avoid implementing heat countermeasures while adopting measures against infectious diseases. This study aimed to clarify the issues faced with heat countermeasures and report on heat acclimation training and cooling support efforts, considering measures against infectious diseases.
Keywords	cooling ; COVID-19 ; elite athletes ; heat acclimation ; Olympics ; Sports ; GV557-1198.995
Subject code	796
Language	English
Publishing date	2022-06-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Effects of Water Temperature during Foot Bath in Young Females.

Nishimura, Masahiro / Tatsuya Saito, Tatsuya Saito / Kato, Toshiaki / Onodera, Sho

Yonago acta medica

2013 Volume 56, Issue 3, Page(s) 79–80

Abstract: We examined the effects of environmental and water temperatures of foot baths on pulse rate, blood pressure, mean skin temperature, salivary amylase (SA) activity, relaxation level and thermal sensation during winter. Five females participated in the ... ...

Abstract	We examined the effects of environmental and water temperatures of foot baths on pulse rate, blood pressure, mean skin temperature, salivary amylase (SA) activity, relaxation level and thermal sensation during winter. Five females participated in the study. The subjects rested in a chair for 20 min and the above-noted physiological reactions during the last 5 min of the resting period were recorded as baseline (BASE) values. Next, the subjects received a 15-min foot bath in water at 40 °C (WT40) or 45 °C (WT45), with a 15-min recovery period. Although SA is thought to be an indicator of stress via the sympathetic nervous system, we did not find a correlation between SA activity and relaxation state. We considered the possible effect of seasonal variation on the physiological reaction to foot bathing.
Language	English
Publishing date	2013-09-11
Publishing country	Japan
Document type	Journal Article
ZDB-ID	840719-8
ISSN	1346-8049 ; 0513-5710
ISSN (online)	1346-8049
ISSN	0513-5710
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Can Muscle Mass Be Maintained with A Simple Resistance Intervention in the Older People? A Cluster Randomized Controlled Trial in Thailand

Bumnet Saengrut / Takeshi Yoda / Yumi Kimura / Yasuko Ishimoto / Rujee Rattanasathien / Tatsuya Saito / Kanlaya Chunjai / Kensaku Miyamoto / Kawin Sirimuengmoon / Rujirat Pudwan / Hironobu Katsuyama

International Journal of Environmental Research and Public Health, Vol 19, Iss 140, p

2022 Volume 140

Abstract: The aging population is rapidly increasing worldwide. Sarcopenia is a common and important health problem among older people. The prevalence of sarcopenia among older Thai adults is increasing. Exercise intervention for sarcopenia prevention may ... ...

Abstract	The aging population is rapidly increasing worldwide. Sarcopenia is a common and important health problem among older people. The prevalence of sarcopenia among older Thai adults is increasing. Exercise intervention for sarcopenia prevention may significantly improve muscle strength, body balance, and muscle mass. Therefore, this study aimed to investigate the effects of a simple resistance intervention (SRI) program in preventing sarcopenia on physiological outcomes among community-dwelling older Thai adults. This study was a 12-week randomized controlled trial, which included 80 community-dwelling older adults in Chiang Mai, Thailand, who were randomly assigned into control (40 participants who performed usual exercise) and intervention (40 participants who performed the SRI program) groups. The SRI program was a home-based program consisting of 30 min of resistance exercise three times/week for 12 weeks, health education on sarcopenia. After 12 weeks, all physiological outcomes were measured and were significantly improved in the intervention group compared with baseline; hand grip, skeletal muscle mass index, and walking speed were significantly improved in the intervention group compared with the control group. Based on our results, the SRI program may prevent muscle weakness in community-dwelling older people in Thailand.
Keywords	sarcopenia ; simple resistance ; older people ; community-dwelling ; Medicine ; R
Subject code	796
Language	English
Publishing date	2022-12-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Enhanced Autophagy in Polycystic Kidneys of AQP11 Null Mice

Yasuko Tanaka / Mayumi Watari / Tatsuya Saito / Yoshiyuki Morishita / Kenichi Ishibashi

International Journal of Molecular Sciences, Vol 17, Iss 12, p

2016 Volume 1993

Abstract: Aquaporin-11 (AQP11) is an intracellular water channel expressed at the endoplasmic reticulum (ER) of the proximal tubule. Its gene disruption in mice leads to intracellular vacuole formation at one week and the subsequent development of polycystic ... ...

Abstract	Aquaporin-11 (AQP11) is an intracellular water channel expressed at the endoplasmic reticulum (ER) of the proximal tubule. Its gene disruption in mice leads to intracellular vacuole formation at one week and the subsequent development of polycystic kidneys by three weeks. As the damaged proximal tubular cells with intracellular vacuoles form cysts later, we postulated that autophagy may play a role in the cyst formation and examined autophagy activity before and after cyst development in AQP11(−/−) kidneys. PCR analysis showed the increased expression of the transcript encoding LC3 (Map1lc3b) as well as other autophagy-related genes in AQP11(−/−) mice. Using green fluorescent protein (GFP)-LC3 transgenic mice and AQP11(−/−) mice, we found that the number of GFP-LC3–positive puncta was increased in the proximal tubule of AQP11(−/−) mice before the cyst formation. Interestingly, they were also observed in the cyst-lining epithelial cell. Further PCR analyses revealed the enhanced expression of apoptosis-related and ER stress–related caspase genes before and after the cyst formation, which may cause the enhanced autophagy. These results suggest the involvement of autophagy in the development and maintenance of kidney cysts in AQP11(−/−) mice.
Keywords	proximal tubule ; LC3 ; ER stress ; apoptosis ; PKD ; Chemistry ; QD1-999 ; Science ; Q ; Biology (General) ; QH301-705.5
Language	English
Publishing date	2016-11-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Recommendation Method Using Bicluster Network Method

Tatsuya Saito / Kohei Kawahara / Yoshifumi Okada

Lecture Notes in Engineering and Computer Science, Vol 2202, Iss 1, Pp 85-

2013 Volume 88

Keywords	Electronic computers. Computer science ; QA75.5-76.95 ; Instruments and machines ; QA71-90 ; Mathematics ; QA1-939 ; Science ; Q
Language	English
Publishing date	2013-03-01T00:00:00Z
Publisher	Newswood and International Association of Engineers
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Water-in-CO₂ Microemulsions Stabilized by Fluorinated Cation–Anion Surfactant Pairs

Sagisaka, Masanobu / Tatsuya Saito / Atsushi Yoshizawa / Sarah E. Rogers / Frédéric Guittard / Christopher Hill / Julian Eastoe / Marijana Blesic

Langmuir. 2019 Feb. 11, v. 35, no. 9

2019

Abstract: High-water-content water-in-supercritical CO₂ (W/CO₂) microemulsions are considered to be green, universal solvents, having both polar and nonpolar domains. Unfortunately, these systems generally require environmentally unacceptable stabilizers like long ...

Abstract	High-water-content water-in-supercritical CO₂ (W/CO₂) microemulsions are considered to be green, universal solvents, having both polar and nonpolar domains. Unfortunately, these systems generally require environmentally unacceptable stabilizers like long and/or multifluorocarbon-tail surfactants. Here, a series of catanionic surfactants having more environmentally friendly fluorinated C₄–C₆ tails have been studied in terms of interfacial properties, aggregation behavior, and solubilizing power in water and/or CO₂. Surface tensions and critical micelle concentrations of these catanionic surfactants are, respectively, lowered by ∼9 mN/m and 100 times than those of the constituent single fluorocarbon-tail surfactants. Disklike micelles in water were observed above the respective critical micelle concentrations, implying the catanionic surfactants have a high critical packing parameter, which should be suitable for the formation of reverse micelles. Based on visual observation of phase behavior and Fourier transform infrared spectroscopic and small-angle neutron scattering studies, one of the three catanionic surfactants tested was found to form transparent single-phase W/CO₂ microemulsions with a water-to-surfactant molar ratio of up to ∼50. This is the first successful demonstration of the formation of W/CO₂ microemulsions by synergistic ion-pairing of anionic and cationic single-tail surfactants. This indicates that catanionic surfactants offer a promising approach to generate high-water-content W/CO₂ microemulsions.
Keywords	Fourier transform infrared spectroscopy ; carbon dioxide ; emulsions ; micelles ; neutron diffraction ; solubilization ; solvents ; stabilizers ; surface tension ; surfactants
Language	English
Dates of publication	2019-0211
Size	p. 3445-3454.
Publishing place	American Chemical Society
Document type	Article
ZDB-ID	2005937-1
ISSN	1520-5827 ; 0743-7463
ISSN (online)	1520-5827
ISSN	0743-7463
DOI	10.1021/acs.langmuir.8b03942
Database	NAL-Catalogue (AGRICOLA)

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