LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 2 of total 2

Search options

  1. Article: Structural Analysis of a Novel Class of R–M Controller Proteins: C.Csp231I from Citrobacter sp. RFL231

    McGeehan, J.E / Streeter, S.D / Thresh, S.-J / Taylor, J.E.N / Shevtsov, M.B / Kneale, G.G

    Journal of molecular biology. 2011 June 3, v. 409, no. 2

    2011  

    Abstract: Controller proteins play a key role in the temporal regulation of gene expression in bacterial restriction–modification (R–M) systems and are important mediators of horizontal gene transfer. They form the basis of a highly cooperative, concentration- ... ...

    Abstract Controller proteins play a key role in the temporal regulation of gene expression in bacterial restriction–modification (R–M) systems and are important mediators of horizontal gene transfer. They form the basis of a highly cooperative, concentration-dependent genetic switch involved in both activation and repression of R–M genes. Here we present biophysical, biochemical, and high-resolution structural analysis of a novel class of controller proteins, exemplified by C.Csp231I. In contrast to all previously solved C-protein structures, each protein subunit has two extra helices at the C-terminus, which play a large part in maintaining the dimer interface. The DNA binding site of the protein is also novel, having largely AAAA tracts between the palindromic recognition half-sites, suggesting tight bending of the DNA. The protein structure shows an unusual positively charged surface that could form the basis for wrapping the DNA completely around the C-protein dimer.
    Keywords Citrobacter ; DNA ; binding sites ; gene expression regulation ; genes ; horizontal gene transfer ; protein subunits ; proteins
    Language English
    Dates of publication 2011-0603
    Size p. 177-188.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 80229-3
    ISSN 1089-8638 ; 0022-2836
    ISSN (online) 1089-8638
    ISSN 0022-2836
    DOI 10.1016/j.jmb.2011.03.033
    Database NAL-Catalogue (AGRICOLA)

    Full text online

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 4' 63/108: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  2. Article: Structural Analysis of a Novel Class of R–M Controller Proteins: C.Csp231I from Citrobacter sp. RFL231

    McGeehan, J.E. / Streeter, S.D. / Thresh, S.-J. / Taylor, J.E.N. / Shevtsov, M.B. / Kneale, G.G.

    Journal of molecular biology

    Volume v. 409,, Issue no. 2

    Abstract: Controller proteins play a key role in the temporal regulation of gene expression in bacterial restriction–modification (R–M) systems and are important mediators of horizontal gene transfer. They form the basis of a highly cooperative, concentration- ... ...

    Abstract Controller proteins play a key role in the temporal regulation of gene expression in bacterial restriction–modification (R–M) systems and are important mediators of horizontal gene transfer. They form the basis of a highly cooperative, concentration-dependent genetic switch involved in both activation and repression of R–M genes. Here we present biophysical, biochemical, and high-resolution structural analysis of a novel class of controller proteins, exemplified by C.Csp231I. In contrast to all previously solved C-protein structures, each protein subunit has two extra helices at the C-terminus, which play a large part in maintaining the dimer interface. The DNA binding site of the protein is also novel, having largely AAAA tracts between the palindromic recognition half-sites, suggesting tight bending of the DNA. The protein structure shows an unusual positively charged surface that could form the basis for wrapping the DNA completely around the C-protein dimer.
    Keywords genes ; binding sites ; Citrobacter ; DNA ; horizontal gene transfer ; proteins ; protein subunits ; gene expression regulation
    Language English
    Document type Article
    ISSN 0022-2836
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top