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  1. Article ; Online: Comparing the effectiveness of biosimilar filgrastim (Nivestim®) versus original filgrastim (Neupogen®) for stem cell mobilization in adult and pediatric healthy donors.

    Muffarrej, Duaa / Hashem, Hasan / Tbakhi, Abdelghani / Najjar, Rula

    Journal of oncology pharmacy practice : official publication of the International Society of Oncology Pharmacy Practitioners

    2023  , Page(s) 10781552231171829

    Abstract: Introduction: Filgrastim is used for the mobilization of stem cells in healthy donors. Though several biosimilar filgrastim products have been approved, there is limited literature evaluating biosimilar products for stem cell mobilization. Therefore, we ...

    Abstract Introduction: Filgrastim is used for the mobilization of stem cells in healthy donors. Though several biosimilar filgrastim products have been approved, there is limited literature evaluating biosimilar products for stem cell mobilization. Therefore, we conducted this study to compare the effectiveness of the original filgrastim, Neupogen®, to the biosimilar product, Nivestim®, for stem cell mobilization(SCM) in healthy donors.
    Methods: This was a retrospective study that included all healthy donors: adults and pediatrics, who received Neupogen® or Nivestim® for stem cell mobilization between 2014 and 2016 at a comprehensive cancer center. Donors received filgrastim at a dose of 5 mcg/kg every 12 h for 4 days to achieve the target CD34  +  cell count of 5-10 × 10
    Results: Over the study period, 89 donors received Neupogen® and 68 received Nivestim®. The median age of donors was 19.5 years (3-64) in the Nivestim® group and 15 years (2-16) in the Neupogen® group. The median number of doses required for SCM was eight doses (6-10) in the Nivestim group and eight (6-16) in the Neupogen® group.
    Conclusion: Biosimilar Nivestim® was as effective as the original, Neupogen®, for stem cell mobilization for healthy adult and pediatric donors. Larger randomized studies are necessary to evaluate the safety and transplant outcomes of the use of Nivestim®.
    Language English
    Publishing date 2023-06-26
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 1330764-2
    ISSN 1477-092X ; 1078-1552
    ISSN (online) 1477-092X
    ISSN 1078-1552
    DOI 10.1177/10781552231171829
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: BCL11A gene over-expression in high risk neuroblastoma.

    Sultan, Iyad / Tbakhi, Abdelghani

    Cancer genetics

    2020  Volume 244, Page(s) 30–31

    MeSH term(s) Biomarkers, Tumor/genetics ; Biomarkers, Tumor/metabolism ; Gene Expression Profiling ; Humans ; N-Myc Proto-Oncogene Protein/genetics ; N-Myc Proto-Oncogene Protein/metabolism ; Neuroblastoma/genetics ; Neuroblastoma/metabolism ; Neuroblastoma/pathology ; Prognosis ; Repressor Proteins/genetics ; Repressor Proteins/metabolism ; Risk Factors ; Survival Rate
    Chemical Substances BCL11A protein, human ; Biomarkers, Tumor ; MYCN protein, human ; N-Myc Proto-Oncogene Protein ; Repressor Proteins
    Language English
    Publishing date 2020-02-27
    Publishing country United States
    Document type Letter
    ZDB-ID 2599227-2
    ISSN 2210-7762
    ISSN 2210-7762
    DOI 10.1016/j.cancergen.2020.02.003
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Book ; Online: Linear Approximate Pattern Matching Algorithm

    Al-okaily, Anas / Tbakhi, Abdelghani

    2021  

    Abstract: Pattern matching is a fundamental process in almost every scientific domain. The problem involves finding the positions of a given pattern (usually of short length) in a reference stream of data (usually of large length). The matching can be an exact or ... ...

    Abstract Pattern matching is a fundamental process in almost every scientific domain. The problem involves finding the positions of a given pattern (usually of short length) in a reference stream of data (usually of large length). The matching can be an exact or as an approximate (inexact). Exact matching is to search for the pattern without allowing for mismatches (or insertions and deletions) of one or more characters in the pattern), while approximate matching is the opposite. For exact matching, several data structures that can be built in linear time and space are used and in practice nowadays. For approximate matching, the solutions proposed to solve this matching are non-linear and currently impractical. In this paper, we designed and implemented a structure that can be built in linear time and space ($O(n)$) and solves the approximate matching problem in $O(m + \frac {log_2n {(log_\Sigma n)} ^{k+1}}{k!} + occ)$ search costs, where $m$ is the length of the pattern, $n$ is the length of the reference, and $k$ is the number of tolerated mismatches (and insertion and deletions).

    Comment: 15 pages double spaced
    Keywords Computer Science - Data Structures and Algorithms ; Computer Science - Databases
    Subject code 005
    Publishing date 2021-10-26
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Impact of the SARS-CoV-2 pandemic on the overall respiratory viruses' transmission in a cancer care setting.

    Mubarak, Sawsan / Alsmadi, Osama / Tbakhi, Abdelghani / Ata, Osama Abu / Hassan, Ala'a / AlGhawrie, Hadeel

    Immunity, inflammation and disease

    2023  Volume 11, Issue 11, Page(s) e1073

    Abstract: Introduction and objective: The emergence of the COVID-19 pandemic raised questions about the interaction between severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and other respiratory viruses. The objective of this study is to validate the ... ...

    Abstract Introduction and objective: The emergence of the COVID-19 pandemic raised questions about the interaction between severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and other respiratory viruses. The objective of this study is to validate the impact of the SARS-CoV-2 pandemic and its interventional measures on the respiratory viruses' transmission/infection rates.
    Methods: A retrospective chart review was conducted for cancer patients who underwent laboratory-confirmed respiratory virus polymerase chain reaction (PCR) testing from January 2018 to June 2022. COVID-19 PCR tests from March 2020 to June 2022 were also included. Joinpoint regression analysis was applied to evaluate trends in respiratory virus rates. Statistical analysis was performed using Statistical Package for Social Science software.
    Results: A total of 6298 respiratory virus PCRs and 40,000 COVID-19 PCRs were performed. Data showed a significant decrease in respiratory viruses' positive cases, total respiratory tests, and respiratory viruses' activity during the pandemic period compared with the pre-pandemic period (p = .0209, .026, and .028, respectively). The joinpoint regression analysis showed a significant decrease of 13.85% in the tested positive cases of respiratory viruses between the years 2018 and 2022. Monthly, the analysis indicated a significant decrease in the positive cases by 13.46% from December 2019 to May 2021. Weekly analysis following lockdown initiation showed a reduction in respiratory virus cases.
    Conclusion: This study provides valuable insights into the interplay between COVID-19 and other respiratory viruses, suggesting that the measures taken for COVID-19 were effective in reducing the spread of viral respiratory infections, aiding future infection control strategies to protect vulnerable populations, including cancer patients, from seasonal respiratory infections.
    MeSH term(s) Humans ; SARS-CoV-2 ; COVID-19/epidemiology ; Pandemics ; Retrospective Studies ; Communicable Disease Control ; Respiratory Tract Infections/epidemiology ; Neoplasms/epidemiology
    Language English
    Publishing date 2023-11-29
    Publishing country England
    Document type Journal Article
    ZDB-ID 2740382-8
    ISSN 2050-4527 ; 2050-4527
    ISSN (online) 2050-4527
    ISSN 2050-4527
    DOI 10.1002/iid3.1073
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Obstacles and Considerations Related to Clinical Trial Research During the COVID-19 Pandemic.

    Hashem, Hasan / Abufaraj, Mohammad / Tbakhi, Abdelghani / Sultan, Iyad

    Frontiers in medicine

    2020  Volume 7, Page(s) 598038

    Abstract: The response to the COVID-19 pandemic from the research and science community has been vigorous, with information being released faster than that of any other event in human history. Articles related to the virus were being rapidly published by January ... ...

    Abstract The response to the COVID-19 pandemic from the research and science community has been vigorous, with information being released faster than that of any other event in human history. Articles related to the virus were being rapidly published by January 2020. A small fraction of these publications comprised reports of prospective clinical trials (0.25%), and many of these trials have imparted conflicting conclusions, leading to confusion among the public and the scientific community. Additionally, the pandemic has raised many serious scientific and ethical concerns related to clinical research. In this review, we divided the conduct of clinical research trials into three steps and critically reviewed each step, along with the challenges and obstacles arising amid the ongoing crisis. The clinical research steps we reviewed include (1) clinical trial design factors such as social and scientific value, feasibility, single vs. multicenter trials, randomization, control groups, endpoints, off-label and compassionate use of medications, data analysis, and verifying the integrity of data; (2) ethical issues such as committee approvals, efficiency, virtual visits and remote monitoring, informed consent, shipping investigational products, and external monitoring and audits; and (3) publication and sharing of preprints, press releases, social media, and misinformation. The COVID-19 pandemic is adversely affecting existing clinical trials for other ailments and diseases, including cancer, with most trials being delayed or deferred. Although urgency is needed to communicate effective treatment and prevention strategies for COVID-19, research efforts should maintain the same high-quality core ethical principles that governed human subject research before the pandemic. Despite the catastrophic devastation caused by the pandemic, the adoption of more flexible, cost-effective methods of conducting clinical trials (without compromising ethical conduct, safety, or data integrity, while maintaining research efficiency) represents a potential silver lining. Streamlining clinical research will help to congruently address other important health issues, despite the ongoing COVID-19 crisis.
    Language English
    Publishing date 2020-12-23
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2775999-4
    ISSN 2296-858X
    ISSN 2296-858X
    DOI 10.3389/fmed.2020.598038
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Flow cytometric signature of CD371-positive B-cell acute lymphoblastic leukemia.

    Shakah, Hind / Tbakhi, Abdelghani / Khudirat, Saleh / Abweh, Ruba Al / Hasasna, Nabil / Alwhaidi, Alaa / Khoujah, Abdallah / Barakat, Fareed

    The Journal of international medical research

    2023  Volume 51, Issue 10, Page(s) 3000605231203842

    Abstract: Objectives: Multiparametric flow cytometry (MFC) aids in the diagnosis and management of B-cell acute lymphoblastic leukemia (B-ALL) by establishing a baseline immunophenotype for leukemic cells and measuring minimal residual disease (MRD) throughout ... ...

    Abstract Objectives: Multiparametric flow cytometry (MFC) aids in the diagnosis and management of B-cell acute lymphoblastic leukemia (B-ALL) by establishing a baseline immunophenotype for leukemic cells and measuring minimal residual disease (MRD) throughout the course of treatment. Aberrant expression patterns of myeloid markers in B-ALL are also examined during long-term surveillance. Here, we investigated the utility of the newly described myeloid marker cluster of differentiation (CD)371 in MRD surveillance via MFC in patients with CD371-positive B-ALL.
    Methods: Eight-color MFC with standard panels (including CD371) was used to evaluate 238 patients with newly diagnosed B-ALL. Expression levels of key markers were retrospectively assessed at diagnosis, as well as days 15 and 33 of therapy.
    Results: CD371 was expressed in 8.4% of patients with B-ALL. CD371 positivity was associated with older age at diagnosis, higher expression levels of CD34 and CD38, and lower expression levels of CD10 and CD20. Residual leukemic cells demonstrated decreased CD10 expression and increased CD45 expression after therapy, whereas CD371 expression remained stable.
    Conclusions: Patients with CD371-positive B-ALL exhibit a specific signature that merits further analysis, particularly because it has been associated with DUX4 rearrangement.
    MeSH term(s) Humans ; Flow Cytometry ; Retrospective Studies ; Precursor B-Cell Lymphoblastic Leukemia-Lymphoma/diagnosis ; Precursor B-Cell Lymphoblastic Leukemia-Lymphoma/genetics ; Burkitt Lymphoma ; Antigens, CD34 ; Neoplasm, Residual/genetics
    Chemical Substances Antigens, CD34
    Language English
    Publishing date 2023-10-11
    Publishing country England
    Document type Journal Article
    ZDB-ID 184023-x
    ISSN 1473-2300 ; 0300-0605 ; 0142-2596
    ISSN (online) 1473-2300
    ISSN 0300-0605 ; 0142-2596
    DOI 10.1177/03000605231203842
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Novel conditioning regimen for upfront haploidentical hematopoietic cell transplantation in children with severe aplastic anemia and donor-specific anti-HLA antibodies.

    Hashem, Hasan / Rihani, Rawad / Shanap, Mayada Abu / Khattab, Eman / Tbakhi, Abdelghani / Sultan, Iyad

    Bone marrow transplantation

    2021  Volume 57, Issue 2, Page(s) 304–305

    MeSH term(s) Anemia, Aplastic/therapy ; Child ; Graft vs Host Disease ; Hematopoietic Stem Cell Transplantation ; Humans ; Transplantation Conditioning
    Language English
    Publishing date 2021-11-27
    Publishing country England
    Document type Letter
    ZDB-ID 632854-4
    ISSN 1476-5365 ; 0268-3369 ; 0951-3078
    ISSN (online) 1476-5365
    ISSN 0268-3369 ; 0951-3078
    DOI 10.1038/s41409-021-01536-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Factors that predict severity of infection and seroconversion in immunocompromised children and adolescents with COVID-19 infection.

    Abu Shanap, Mayada / Sughayer, Maher / Alsmadi, Osama / Elzayat, Ismail / Al-Nuirat, Abeer / Tbakhi, Abdelghani / Sultan, Iyad

    Frontiers in immunology

    2022  Volume 13, Page(s) 919762

    Abstract: Objectives: We aimed to study the outcomes, severity, and seroconversion post SARS-CoV-2 infection in immunocompromised children and adolescents treated at our center.: Method: For this observational study, all pediatric patients who had COVID-19 ... ...

    Abstract Objectives: We aimed to study the outcomes, severity, and seroconversion post SARS-CoV-2 infection in immunocompromised children and adolescents treated at our center.
    Method: For this observational study, all pediatric patients who had COVID-19 infection from Sep-22-2020 to Nov-10-2021were identified by reviewing our laboratory records. Their charts were reviewed to determine clinical severity and outcome. Blood samples were drawn for anti-SARS-CoV-2 antibody assay. Serious COVID-19 infection (SVI) was defined if the patient had moderate, severe, or critical illness. A cutoff of 100 U/mL anti-SARS-CoV-2 antibodies was used to categorize low and high titer seroconversion.
    Results: We identified 263 pediatric patients with COVID-19; most (68%) were symptomatic: 5% had severe or critical infection, 25% were hospitalized, 12 required respiratory support, 12 were admitted to the ICU, and five patients (2%) died. Multivariable analysis revealed several factors that predict SVI: Age above 12 years (p=0.035), body mass index above 95
    Conclusions: SARS-CoV-2 antibodies developed in most immunocompromised patients with COVID-19 infection in our study. Mortality was relatively low in our patients. Our univariable and multivariable models showed multiple variables that predict severity of infections and antibody response post COVID-19 infection. These observations may guide choice of active therapy during infection and the best timing of vaccination in this high-risk population.
    MeSH term(s) Adolescent ; Antibodies, Viral ; COVID-19 ; Child ; Hematologic Neoplasms ; Humans ; Immunocompromised Host ; SARS-CoV-2 ; Seroconversion
    Chemical Substances Antibodies, Viral
    Language English
    Publishing date 2022-08-03
    Publishing country Switzerland
    Document type Journal Article ; Observational Study
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2022.919762
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Programmed Death Ligand-1 is Frequently Expressed in Primary Acute Myeloid Leukemia and B-Acute Lymphoblastic Leukemia.

    Hamdan, Sara O / Sughayer, Maher / Khader, Majd / Tbakhi, Abdelghani / Khudirat, Saleh / Hejazi, Ala / AlRyalat, Saifaldeen / Bustami, Nadwa / Aladily, Tariq N

    Clinical laboratory

    2022  Volume 68, Issue 4

    Abstract: Background: The introduction of checkpoint inhibitors in solid cancer therapy showed successful results. The role of Programmed Death-1/Programmed Death-Ligand 1 (PD1/PD-L1) in hematologic malignancies is currently being investigated and clinical trials ...

    Abstract Background: The introduction of checkpoint inhibitors in solid cancer therapy showed successful results. The role of Programmed Death-1/Programmed Death-Ligand 1 (PD1/PD-L1) in hematologic malignancies is currently being investigated and clinical trials are ongoing. Preliminary findings showed conflicting results. In this study, we examined the degree of PD-L1 and PD-L2 expression in primary acute leukemia patients.
    Methods: Flow cytometry expression of PD-L1 and PD-L2 was evaluated in de novo acute leukemia in the collaborating institutions between 2018 - 2020.
    Results: One hundred forty patients were identified. PD-L1 was positive in 34/70 (49%) of AML, 25/50 (50%) of B-ALL, and none (0/20) of T-ALL patients. In contrast, PD-L2 was solely expressed in eight (19%) AML patients. The expression of PD-L1 showed statistically significant correlation with the type of acute leukemia (AML and B-ALL > T-ALL, p < 0.001) and with age group (adults > children, p = 0.048), but not with blast count, immunophenotype or cytogenetic mutations. The positivity for PD-L1 was associated with worse overall survival in AML, but not in B-ALL.
    Conclusions: The expression of PD-L1 is common among newly diagnosed AML and B-ALL patients and is not restricted to relapsed cases as previously described. PD-L2 is less commonly expressed and is accompanied by PD-L1 expression. Positive PD-L1 patients may benefit from treatment with immune checkpoint inhibitors, especially in AML. Further studies are recommended.
    MeSH term(s) Acute Disease ; Adult ; B7-H1 Antigen ; Child ; Humans ; Leukemia, Myeloid, Acute/diagnosis ; Leukemia, Myeloid, Acute/drug therapy ; Leukemia, Myeloid, Acute/genetics ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
    Chemical Substances B7-H1 Antigen ; CD274 protein, human
    Language English
    Publishing date 2022-04-17
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1307629-2
    ISSN 1433-6510 ; 0941-2131
    ISSN 1433-6510 ; 0941-2131
    DOI 10.7754/Clin.Lab.2021.210701
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Haploidentical Hematopoietic Cell Transplantation Using Post-transplant Cyclophosphamide for Children with Non-malignant Diseases.

    Hashem, Hasan / Najjar, Rula / Abu-Shanap, Mayada / Khattab, Eman / Rihani, Rawad / Tbakhi, Abdelghani / Sultan, Iyad

    Journal of clinical immunology

    2021  Volume 41, Issue 8, Page(s) 1754–1761

    Abstract: Haploidentical hematopoietic cell transplantation (HCT) is a valuable curative option for children with non-malignant diseases. Haploidentical HCT using post-transplant cyclophosphamide (PTCy) is a readily available option in the absence of an HLA- ... ...

    Abstract Haploidentical hematopoietic cell transplantation (HCT) is a valuable curative option for children with non-malignant diseases. Haploidentical HCT using post-transplant cyclophosphamide (PTCy) is a readily available option in the absence of an HLA-matched donor. We conducted a retrospective single-center study on the outcome of haploidentical HCT in children with non-malignant diseases. We gathered data from 44 patients underwent HCT in the period 2015 to 2020. The indications for HCT were bone marrow failure, primary immunodeficiency, metabolic disorders, and hemoglobinopathy. Median age at HCT was 4 years (range 0.7-20). The conditioning regimens were myeloablative (n = 17) or reduced intensity (n = 27). After a median follow-up of 20 months (range 4-71), 2-year overall survival was 89% and 2-year GvHD-free relapse-free survival (GRFS) was 66%. Incidence of primary graft failure was 13.6%. Cumulative incidence of grade II-IV acute and moderate/severe chronic GvHD were 20% and 6.4%, respectively. Younger age at HCT (< 4 years) and primary immunodeficiency were significantly associated with better GRFS (p < 0.05). In conclusion, haploidentical HCT using PTCy is feasible and curative in children with non-malignant diseases lacking an HLA-matched donor. Early diagnosis and referral in addition to timely treatment can further improve outcomes.
    MeSH term(s) Adolescent ; Adult ; Child ; Child, Preschool ; Cyclophosphamide/therapeutic use ; Female ; Graft vs Host Disease ; Hematologic Diseases/therapy ; Hematopoietic Stem Cell Transplantation ; Humans ; Immunosuppressive Agents/therapeutic use ; Infant ; Male ; Metabolic Diseases/therapy ; Primary Immunodeficiency Diseases/therapy ; Retrospective Studies ; Transplantation, Haploidentical ; Young Adult
    Chemical Substances Immunosuppressive Agents ; Cyclophosphamide (8N3DW7272P)
    Language English
    Publishing date 2021-08-06
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 779361-3
    ISSN 1573-2592 ; 0271-9142
    ISSN (online) 1573-2592
    ISSN 0271-9142
    DOI 10.1007/s10875-021-01113-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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