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  1. Article: DNA metabarcoding assessment of Neotropical ichthyoplankton communities is marker-dependent.

    Teixeira, Daniel Fonseca / Hilário, Heron Oliveira / Santos, Gilmar Bastos / Carvalho, Daniel Cardoso

    Ecology and evolution

    2023  Volume 13, Issue 10, Page(s) e10649

    Abstract: The study of ichthyoplankton is paramount to understanding fish assemblages' reproductive dynamics. DNA metabarcoding has been applied as a rapid, cost-effective, and accurate taxonomy tool, allowing the identification of multiple individuals ... ...

    Abstract The study of ichthyoplankton is paramount to understanding fish assemblages' reproductive dynamics. DNA metabarcoding has been applied as a rapid, cost-effective, and accurate taxonomy tool, allowing the identification of multiple individuals simultaneously. However, there remain significant challenges when using DNA metabarcoding, such as molecular marker choice according to the taxonomic resolution and length of the fragment to be sequenced, primer bias, incomplete reference databases, and qualitative inference incongruences. Here, 30 ichthyoplankton pools collected from a Neotropical river were identified at a molecular level using DNA metabarcoding to compare the resolution, sensibility, specificity, and relative read abundance (RRA) recovery of three molecular markers: the standard COI fragment (650 pb, with each end analyzed individually) and two short 12S rRNA genes markers (≅200 bp - NeoFish and MiFish markers). The combined use of the three markers increased the genera detection rates by 25%-87.5%, allowing an increased taxonomic coverage and robust taxonomic identification of complex Neotropical ichthyoplankton communities. RRA is marker-dependent, indicating caution is still needed while inferring species abundance based on DNA metabarcoding data when using PCR-dependent protocols.
    Language English
    Publishing date 2023-10-20
    Publishing country England
    Document type Journal Article
    ZDB-ID 2635675-2
    ISSN 2045-7758
    ISSN 2045-7758
    DOI 10.1002/ece3.10649
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Invasion dynamics of the white piranha (Serrasalmus brandtii) in a Neotropical river basin

    Teixeira, Daniel Fonseca / Neto, Francisco Ricardo Andrade / Gomes, Laís Carvalho / Beheregaray, Luciano Bellagamba / Carvalho, Daniel Cardoso

    Biological invasions. 2020 Mar., v. 22, no. 3

    2020  

    Abstract: Clarifying the intricate history of unrecorded fish invasions represents an important step in understanding the invasion process. Here, we elucidate the invasion of a Neotropical river basin in Southeastern Brazil by a very efficient predator, the white ... ...

    Abstract Clarifying the intricate history of unrecorded fish invasions represents an important step in understanding the invasion process. Here, we elucidate the invasion of a Neotropical river basin in Southeastern Brazil by a very efficient predator, the white piranha (Serrasalmus brandtii). We used a temporal series of population dynamics data (2008–2016) and analyses of mitochondrial DNA sequences (COI, 16S, and control region) of specimens from the entire native (São Francisco River Basin, Southeastern Brazil) and invaded (Jequitinhonha River Basin, JRB) ranges. We detected low genetic diversity (h = 0.5835) and strong genetic structure in the invasive range (Fst = 0.9141), despite high genetic diversity (h = 0.8900) and low genetic structure (Fst from 0.0740 to 0.1348) in the native range. High genetic structure in the invaded range was associated with a hydroelectric dam that prevented the admixture of two independent introductory acts into the JRB (downstream and upstream of the Irapé Dam). The rapid invasion capability of Serrasalmus brandtii, with few propagules, indicates that the species should be included in ecological risk assessments for restocking efforts of other commercially or ecologically important fish species and dam construction in Brazil. The combination of genetic and population dynamic datasets enabled the reconstruction of a top predator fish invasion in the Neotropics and shed light on ecological factors that influenced its invasion success.
    Keywords Neotropics ; Serrasalmus ; colonizing ability ; dams (hydrology) ; data collection ; fish ; genetic structure ; genetic variation ; mitochondrial DNA ; population dynamics ; risk ; watersheds ; Brazil
    Language English
    Dates of publication 2020-03
    Size p. 983-995.
    Publishing place Springer International Publishing
    Document type Article
    ZDB-ID 1438729-3
    ISSN 1573-1464 ; 1387-3547
    ISSN (online) 1573-1464
    ISSN 1387-3547
    DOI 10.1007/s10530-019-02138-y
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 7608: Show issues

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  3. Article ; Online: Integrative approach detects natural hybridization of sympatric lambaris species and emergence of infertile hybrids.

    Pinheiro, Ana Paula Barbosa / Melo, Rafael Magno Costa / Teixeira, Daniel Fonseca / Birindelli, José Luís Olivan / Carvalho, Daniel Cardoso / Rizzo, Elizete

    Scientific reports

    2019  Volume 9, Issue 1, Page(s) 4333

    Abstract: Despite its relevance for ecology, evolution and conservation of species, natural hybridization and hybrids biology are still poorly studied in freshwater fish. Here, we tested the hypothesis that sympatric species Astyanax paranae and A. fasciatus are ... ...

    Abstract Despite its relevance for ecology, evolution and conservation of species, natural hybridization and hybrids biology are still poorly studied in freshwater fish. Here, we tested the hypothesis that sympatric species Astyanax paranae and A. fasciatus are able to interbreed in the natural environment and presented evidence for the first record of hybridization between these species. We analyzed anatomical traits, gametogenesis, reproductive biology, and genetic variations of the COI and S7 genes of both species and putative hybrids. Intermediate morphometric and meristic features were observed in hybrids when compared to A. paranae and A. fasciatus. Overlap in reproductive season was showed for these species, with greater reproductive activity from August to January, but hybrids did not present any sign of gonadal maturation. Oogonia and perinucleolar follicles as well as spermatogonia and primary spermatocytes were found in hybrids, but previtellogenic and vitellogenic follicles, spermatids, and spermatozoa were absent. Moreover, several alterations in gametogenesis were detected, such as interrupted meiosis in both males and females, vacuolated and degenerated germ cells, increased interstitial tissue, and presence of immune cells. Molecular analyses supported the hypothesis of hybridization between A. paranae and A. fasciatus. Overall, our multidisciplinary approach also provides strong evidence that hybrids are infertile.
    MeSH term(s) Animals ; Characidae/genetics ; Characidae/physiology ; Female ; Haplotypes ; Hybridization, Genetic ; Infertility ; Male ; Reproduction ; Sympatry
    Language English
    Publishing date 2019-03-13
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-019-40856-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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