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  1. Article ; Online: Distributions and expressions of Aquaporin‐5 and 7 in the testes of developing male chicks

    Kara, Hülya / Tekiner, Deniz

    Anatomia, Histologia, Embryologia. 2024 Jan., v. 53, no. 1 p.e12978-

    2024  

    Abstract: Aquaporins (AQPs) are integral membrane proteins that act as water channels for which a total of 13 orthologs of AQP genes in birds have been reported. Tissue expression and cellular or subcellular localization of AQPs have been poorly investigated in ... ...

    Abstract Aquaporins (AQPs) are integral membrane proteins that act as water channels for which a total of 13 orthologs of AQP genes in birds have been reported. Tissue expression and cellular or subcellular localization of AQPs have been poorly investigated in the male reproductive system of birds. We aimed to determine the distribution and localization of AQP5 and AQP7 proteins by immunocytochemistry in testicular tissues obtained from developing chicks (14, 21, 28, 35 and 42 days old). Totally 175 male chicks (Ross 308) were used in the study from which testicular tissue was removed, fixed in 10% formaldehyde solution, then embedded in paraffin blocks. Five μm sections were cut, mounted on poly‐L‐lysine slides, dried in an oven, then dehydrated using standard immunohistochemistry staining protocol. The sections were imaged with a Nikon Eclipse 50i trinocular light microscope. Immunohistochemical evaluation of the immune reactivity of AQP5 revealed a positive immune reaction in spermatocytes and interstitial areas of the testes in 14‐day‐old chicks. Testicular tissue AQP5 immune reactivity was observed in the tubule and the interstitial regions of 21‐, 28‐, 35‐ and 42‐day‐old chicks. AQP7 immune reactions were determined in the tubule and interstitial areas testes of developing chicks' testis tissue, with increasing positivity corresponding to older age. The expression of AQP5 and AQP7 appears to be species‐specific due to differences in localization and expression in male chicks compared with studies of other mammals, which is likely to play an important role in regulating fluid and sperm volume. This research can serve as a base for future studies that will contribute to the understanding of the male genital system of AQPs.
    Keywords aquaporins ; formaldehyde ; immune response ; immunocytochemistry ; immunohistochemistry ; light microscopes ; male genitalia ; males ; ovens ; spermatocytes ; spermatozoa ; testes
    Language English
    Dates of publication 2024-01
    Publishing place John Wiley & Sons, Ltd
    Document type Article ; Online
    Note JOURNAL ARTICLE
    ZDB-ID 537922-2
    ISSN 1439-0264 ; 0340-2096 ; 0044-4294
    ISSN (online) 1439-0264
    ISSN 0340-2096 ; 0044-4294
    DOI 10.1111/ahe.12978
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 960 c: Show issues

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  2. Article ; Online: Distributions and expressions of Aquaporin-5 and 7 in the testes of developing male chicks.

    Kara, Hülya / Tekiner, Deniz

    Anatomia, histologia, embryologia

    2023  Volume 53, Issue 1, Page(s) e12978

    Abstract: Aquaporins (AQPs) are integral membrane proteins that act as water channels for which a total of 13 orthologs of AQP genes in birds have been reported. Tissue expression and cellular or subcellular localization of AQPs have been poorly investigated in ... ...

    Abstract Aquaporins (AQPs) are integral membrane proteins that act as water channels for which a total of 13 orthologs of AQP genes in birds have been reported. Tissue expression and cellular or subcellular localization of AQPs have been poorly investigated in the male reproductive system of birds. We aimed to determine the distribution and localization of AQP5 and AQP7 proteins by immunocytochemistry in testicular tissues obtained from developing chicks (14, 21, 28, 35 and 42 days old). Totally 175 male chicks (Ross 308) were used in the study from which testicular tissue was removed, fixed in 10% formaldehyde solution, then embedded in paraffin blocks. Five μm sections were cut, mounted on poly-L-lysine slides, dried in an oven, then dehydrated using standard immunohistochemistry staining protocol. The sections were imaged with a Nikon Eclipse 50i trinocular light microscope. Immunohistochemical evaluation of the immune reactivity of AQP5 revealed a positive immune reaction in spermatocytes and interstitial areas of the testes in 14-day-old chicks. Testicular tissue AQP5 immune reactivity was observed in the tubule and the interstitial regions of 21-, 28-, 35- and 42-day-old chicks. AQP7 immune reactions were determined in the tubule and interstitial areas testes of developing chicks' testis tissue, with increasing positivity corresponding to older age. The expression of AQP5 and AQP7 appears to be species-specific due to differences in localization and expression in male chicks compared with studies of other mammals, which is likely to play an important role in regulating fluid and sperm volume. This research can serve as a base for future studies that will contribute to the understanding of the male genital system of AQPs.
    MeSH term(s) Male ; Animals ; Testis/metabolism ; Aquaporin 5/metabolism ; Semen ; Spermatozoa ; Chickens ; Aquaporin 1/metabolism ; Mammals
    Chemical Substances Aquaporin 5 ; Aquaporin 1 (146410-94-8)
    Language English
    Publishing date 2023-10-04
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 537922-2
    ISSN 1439-0264 ; 0340-2096 ; 0044-4294
    ISSN (online) 1439-0264
    ISSN 0340-2096 ; 0044-4294
    DOI 10.1111/ahe.12978
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 1221: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Assessment of toxicological effects of favipiravir (T-705) on the lung tissue of rats: An experimental study.

    Erbaş, Elif / Celep, Nevra Aydemir / Tekiner, Deniz / Genç, Aydın / Gedikli, Semin

    Journal of biochemical and molecular toxicology

    2023  Volume 38, Issue 1, Page(s) e23536

    Abstract: This study aimed to present new data on the side effects of favipiravir on healthy lung tissue and the respiratory system. In the study, two different durations (5 and 10 days) were preferred to determine the effect of favipiravir treatment due to ... ...

    Abstract This study aimed to present new data on the side effects of favipiravir on healthy lung tissue and the respiratory system. In the study, two different durations (5 and 10 days) were preferred to determine the effect of favipiravir treatment due to clinical improvement rates of approximately 5 and 10 days during the use of favipiravir in COVID-19 patients. In addition, after 10 days of favipiravir treatment, animals were kept for 5 days without any treatment to determine the regeneration of lung tissues. Favipiravir was administered to rats by oral gavage at a daily dose of 200 mg/kg for 5 and 10 days, as in previous studies. At the end of the experiment, the histopathological and biochemical effects of favipiravir in the lung tissue were investigated. The data obtained from the study showed that favipiravir increased oxidative stress parameters, expression of apoptotic markers, and pro-inflammatory markers in lung tissue. Since malondialdehydes is an oxidant parameter, it increased in favipiravir-administered groups; It was determined that the antioxidant parameters glutathione, superoxide dismutase, glutathione peroxidase, and catalase decreased. Other markers used in the analysis are Bcl-2, Bax, NF-κB, interleukin (IL)-6, Muc1, iNOS, P2X7R, IL-6 and caspase-3. The levels of Bax, caspase-3, NF-κB, IL-6, Muc1, and P2X7R were increased in the Fav-treated groups compared with the control. However, the levels of Bcl-2 decreased in the Fav-treated groups. The present study proves that favipiravir, widely used today, causes side effects in lung tissue.
    MeSH term(s) Humans ; Rats ; Animals ; Caspase 3/metabolism ; NF-kappa B/metabolism ; bcl-2-Associated X Protein/metabolism ; Interleukin-6/metabolism ; Antioxidants/pharmacology ; Oxidative Stress ; Lung/metabolism ; Proto-Oncogene Proteins c-bcl-2/metabolism ; Apoptosis ; Amides ; Pyrazines
    Chemical Substances favipiravir (EW5GL2X7E0) ; Caspase 3 (EC 3.4.22.-) ; NF-kappa B ; bcl-2-Associated X Protein ; Interleukin-6 ; Antioxidants ; Proto-Oncogene Proteins c-bcl-2 ; Amides ; Pyrazines
    Language English
    Publishing date 2023-11-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1410020-4
    ISSN 1099-0461 ; 1095-6670
    ISSN (online) 1099-0461
    ISSN 1095-6670
    DOI 10.1002/jbt.23536
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2201: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article: Immunohistochemical distribution of glucagon -, insulin -, somatostatin -, gastrin-,and serotonin-containing cells in the pancreas of the Van cat

    KARACA, Turan / KARA, Adem / ŞİMŞEK, Nejdet / USLU, Sema / TEKİNER, Deniz / YÖRÜK, Mecit

    Turkish Journal of Veterinary and Animal Sciences; Year: 2014 Volume: 38 Issue: 3; 204-311

    Abstract: The regional distribution, relative frequency, and appearance of glucagon (A-cell)-, insulin (B-cell)-, somatostatin (D-cell)-, gastrin (G-cell)-, and serotonin (EC-cell)-secreting cells in the endocrine and exocrine pancreas of Van cats were examined ... ...

    Abstract The regional distribution, relative frequency, and appearance of glucagon (A-cell)-, insulin (B-cell)-, somatostatin (D-cell)-, gastrin (G-cell)-, and serotonin (EC-cell)-secreting cells in the endocrine and exocrine pancreas of Van cats were examined using the immunohistochemistry method. Glucagon immunopositive A-cells were principally found in the central region of the islets of Langerhans, while insulin immunopositive B-cells were located in the periphery of the pancreatic islets. Moreover, several A- and B-cells were observed as only single cells or clusters of 2 to 3 immunopositive cells in the exocrine parenchyma and the pancreatic duct epithelium. Somatostatin and gastrin immunopositive reactivities were negligible in the peripheral regions of the pancreatic islets of Langerhans and in the exocrine parenchyma. However, serotonin-immunopositive EC-cells were observed in neither the endocrine islets nor any other sites of the tissue. The existence, regional distribution, and relative frequency of A-, B-, D-, G- and EC-cells in the pancreas of Van cats have been analyzed in this study for the first time. The immunopositivity and distribution of endocrine cells in the Van cat pancreas were determined to be partially different from those of other carnivorous species such as dogs and other cats.
    Language English
    Document type Article
    ISSN 1303-6181
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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