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  1. Article ; Online: Novel hepatoviruses in synanthropic bats in the upper Midwestern United States.

    Temeeyasen, Gun / Hause, Ben M

    Archives of virology

    2022  Volume 167, Issue 12, Page(s) 2749–2751

    Abstract: A nearly complete genome sequence of hepatovirus G was isolated from an Eptesicus fuscus bat submitted for rabies virus testing due to human exposure in South Dakota. The predicted polyprotein sequence was 78.2% and 74.4% identical to genotypes G1 and G2, ...

    Abstract A nearly complete genome sequence of hepatovirus G was isolated from an Eptesicus fuscus bat submitted for rabies virus testing due to human exposure in South Dakota. The predicted polyprotein sequence was 78.2% and 74.4% identical to genotypes G1 and G2, respectively, recovered from bats in Ghana. Quantitative PCR on 90 E. fuscus bats showed that eight (8.9%) were positive for hepatovirus G. Targeted sequencing of the VP2 region of the genome for five positive samples showed >99% identity to hepatovirus G strain Ef15893, demonstrating that hepatovirus G commonly circulates in E. fuscus bats in the upper Midwest.
    MeSH term(s) Animals ; Humans ; Chiroptera ; Rabies ; Hepatovirus ; Rabies virus ; Midwestern United States/epidemiology
    Language English
    Publishing date 2022-09-22
    Publishing country Austria
    Document type Journal Article
    ZDB-ID 7491-3
    ISSN 1432-8798 ; 0304-8608
    ISSN (online) 1432-8798
    ISSN 0304-8608
    DOI 10.1007/s00705-022-05610-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Novel hepatoviruses in synanthropic bats in the upper Midwestern United States

    Temeeyasen, Gun / Hause, Ben M.

    Arch Virol. 2022 Dec., v. 167, no. 12 p.2749-2751

    2022  

    Abstract: A nearly complete genome sequence of hepatovirus G was isolated from an Eptesicus fuscus bat submitted for rabies virus testing due to human exposure in South Dakota. The predicted polyprotein sequence was 78.2% and 74.4% identical to genotypes G1 and G2, ...

    Abstract A nearly complete genome sequence of hepatovirus G was isolated from an Eptesicus fuscus bat submitted for rabies virus testing due to human exposure in South Dakota. The predicted polyprotein sequence was 78.2% and 74.4% identical to genotypes G1 and G2, respectively, recovered from bats in Ghana. Quantitative PCR on 90 E. fuscus bats showed that eight (8.9%) were positive for hepatovirus G. Targeted sequencing of the VP2 region of the genome for five positive samples showed >99% identity to hepatovirus G strain Ef15893, demonstrating that hepatovirus G commonly circulates in E. fuscus bats in the upper Midwest.
    Keywords Eptesicus fuscus ; Rabies lyssavirus ; genome ; humans ; nucleotide sequences ; polyproteins ; quantitative polymerase chain reaction ; synanthropes ; Ghana ; South Dakota
    Language English
    Dates of publication 2022-12
    Size p. 2749-2751.
    Publishing place Springer Vienna
    Document type Article ; Online
    ZDB-ID 7491-3
    ISSN 1432-8798 ; 0304-8608
    ISSN (online) 1432-8798
    ISSN 0304-8608
    DOI 10.1007/s00705-022-05610-8
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Geographical Expansion of Avian Metapneumovirus Subtype B: First Detection and Molecular Characterization of Avian Metapneumovirus Subtype B in US Poultry.

    Luqman, Muhammad / Duhan, Naveen / Temeeyasen, Gun / Selim, Mohamed / Jangra, Sumit / Mor, Sunil Kumar

    Viruses

    2024  Volume 16, Issue 4

    Abstract: Avian metapneumovirus (aMPV), classified within ... ...

    Abstract Avian metapneumovirus (aMPV), classified within the
    MeSH term(s) Animals ; Metapneumovirus/genetics ; Metapneumovirus/classification ; Metapneumovirus/isolation & purification ; Paramyxoviridae Infections/veterinary ; Paramyxoviridae Infections/virology ; Paramyxoviridae Infections/epidemiology ; Poultry Diseases/virology ; Poultry Diseases/epidemiology ; Phylogeny ; Turkeys/virology ; Genome, Viral ; United States/epidemiology ; Chickens/virology ; Poultry/virology ; Metagenomics ; Disease Outbreaks/veterinary
    Language English
    Publishing date 2024-03-26
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v16040508
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Alphacoronaviruses Are Common in Bats in the Upper Midwestern United States

    Schaeffer, Reagan / Temeeyasen, Gun / Hause, Ben M.

    Viruses. 2022 Jan. 19, v. 14, no. 2

    2022  

    Abstract: Bats are a reservoir for coronaviruses (CoVs) that periodically spill over to humans, as evidenced by severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2. A collection of 174 bat samples originating from South Dakota, Minnesota, Iowa, ...

    Abstract Bats are a reservoir for coronaviruses (CoVs) that periodically spill over to humans, as evidenced by severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2. A collection of 174 bat samples originating from South Dakota, Minnesota, Iowa, and Nebraska submitted for rabies virus testing due to human exposure were analyzed using a pan-coronavirus PCR. A previously partially characterized CoV, Eptesicus bat CoV, was identified in 12 (6.9%) samples by nested RT-PCR. Six near-complete genomes were determined. Genetic analysis found a high similarity between all CoV-positive samples, Rocky Mountain bat CoV 65 and alphacoronavirus HCQD-2020 recently identified in South Korea. Phylogenetic analysis of genome sequences showed EbCoV is closely related to bat CoV HKU2 and swine acute diarrhea syndrome CoV; however, topological incongruences were noted for the spike gene that was more closely related to porcine epidemic diarrhea virus. Similar to some alphaCoVs, a novel gene, ORF7, was discovered downstream of the nucleocapsid, whose protein lacked similarity to known proteins. The widespread circulation of EbCoV with similarities to bat viruses that have spilled over to swine warrants further surveillance.
    Keywords Eptesicus ; Porcine epidemic diarrhea virus ; Rabies lyssavirus ; Severe acute respiratory syndrome coronavirus ; Severe acute respiratory syndrome coronavirus 2 ; diarrhea ; genes ; genetic analysis ; humans ; monitoring ; nucleocapsid ; phylogeny ; swine ; topology ; Iowa ; Minnesota ; Nebraska ; South Dakota ; South Korea
    Language English
    Dates of publication 2022-0119
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2516098-9
    ISSN 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14020184
    Database NAL-Catalogue (AGRICOLA)

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  5. Article: Five years of porcine circovirus 3: What have we learned about the clinical disease, immune pathogenesis, and diagnosis

    Kroeger, Molly / Temeeyasen, Gun / Piñeyro, Pablo E

    Virus research. 2022 June, v. 314

    2022  

    Abstract: Porcine circovirus type 3 (PCV3) is a non-enveloped, circular, single-stranded DNA virus in the family Circoviridae. This member of the genus Circovirus was initially described as affecting swine in 2016, and new research has provided further insight ... ...

    Abstract Porcine circovirus type 3 (PCV3) is a non-enveloped, circular, single-stranded DNA virus in the family Circoviridae. This member of the genus Circovirus was initially described as affecting swine in 2016, and new research has provided further insight into its structural characteristics, disease presentations, pathogenesis, and immune response following infection. Therefore, this review aims to summarize advances in PCV3-related research about genomic characteristics epidemiology, pathogenesis, immune response, and the development of diagnostics. PCV3 has been detected globally and retrospectively in pigs of all ages and is associated with a range of clinical presentations, including multisystemic inflammatory syndrome, reproductive failure, porcine dermatitis and nephropathy syndrome, and subclinical infection. Experimental studies have successfully reproduced multisystemic inflammation but have not detected clinical disease. These findings, coupled with a large number of reports of coinfections coinciding with PCV3, may suggest that PCV3 infection alone may not be sufficient to cause evidenceable clinical disease. The pathogenesis of PCV3 has not been fully elucidated yet, and while receptors that facilitate cell-viral entry have not been identified, replication has been confirmed in a wide range of cell types, including trophoblasts, myocardiocytes, skin adipocytes, and neurons. PCV3 seems to evade the host immune response as evidenced by persistent viremia 42 days post-infection in experimental and longitudinal field studies despite a strong humoral response. Minimal differences in host cytokine profiles and peripheral cell-mediated responses have been observed, but certainly many questions still surround the mechanisms by which PCV3 evades the immune response. The epidemiology of PCV3 remains unclear, and the exact routes of transmission have not been described; but, PCV3 can be shed in oral fluids, nasal secretions, feces, colostrum, and semen, demonstrating the importance of lateral and vertical transmission. The detection of PCV3 in numerous domesticated and wild animal species, including cattle, dogs, mice, wild boar, chamois, roe deer, ticks, and mosquitoes, suggests the potential for multiple reservoirs and cross-species transmission. Current advances in PCV3 diagnostic tests have the ability to differentiate PCV3 from other PCVs and corroborate its presence within lesions. Given that the economic impact associated with PCV3 infection has not been assessed and the virus has the potential to emerge as a high-prevalence pathogen in the coming years, future research should focus on filling the knowledge gaps identified in this review.
    Keywords Capreolus capreolus ; Porcine circovirus-3 ; adipocytes ; cattle ; chamois ; colostrum ; cytokines ; dermatitis ; diagnostic techniques ; economic impact ; epidemiology ; feces ; genomics ; humoral immunity ; immune response ; inflammation ; kidney diseases ; nose ; pathogenesis ; pathogens ; research ; semen ; single-stranded DNA ; swine ; viremia ; viruses ; wild boars
    Language English
    Dates of publication 2022-06
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 605780-9
    ISSN 1872-7492 ; 0168-1702
    ISSN (online) 1872-7492
    ISSN 0168-1702
    DOI 10.1016/j.virusres.2022.198764
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: Alphacoronaviruses Are Common in Bats in the Upper Midwestern United States.

    Schaeffer, Reagan / Temeeyasen, Gun / Hause, Ben M

    Viruses

    2022  Volume 14, Issue 2

    Abstract: Bats are a reservoir for coronaviruses (CoVs) that periodically spill over to humans, as evidenced by severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2. A collection of 174 bat samples originating from South Dakota, Minnesota, Iowa, ...

    Abstract Bats are a reservoir for coronaviruses (CoVs) that periodically spill over to humans, as evidenced by severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2. A collection of 174 bat samples originating from South Dakota, Minnesota, Iowa, and Nebraska submitted for rabies virus testing due to human exposure were analyzed using a pan-coronavirus PCR. A previously partially characterized CoV,
    MeSH term(s) Alphacoronavirus/classification ; Alphacoronavirus/genetics ; Alphacoronavirus/isolation & purification ; Animals ; Chiroptera/virology ; Disease Reservoirs/veterinary ; Disease Reservoirs/virology ; Genome, Viral ; Iowa ; Midwestern United States ; Minnesota ; Phylogeny ; Republic of Korea ; Sequence Analysis, DNA ; South Dakota ; Viral Zoonoses/transmission
    Language English
    Publishing date 2022-01-19
    Publishing country Switzerland
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14020184
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Five years of porcine circovirus 3: What have we learned about the clinical disease, immune pathogenesis, and diagnosis.

    Kroeger, Molly / Temeeyasen, Gun / Piñeyro, Pablo E

    Virus research

    2022  Volume 314, Page(s) 198764

    Abstract: Porcine circovirus type 3 (PCV3) is a non-enveloped, circular, single-stranded DNA virus in the family Circoviridae. This member of the genus Circovirus was initially described as affecting swine in 2016, and new research has provided further insight ... ...

    Abstract Porcine circovirus type 3 (PCV3) is a non-enveloped, circular, single-stranded DNA virus in the family Circoviridae. This member of the genus Circovirus was initially described as affecting swine in 2016, and new research has provided further insight into its structural characteristics, disease presentations, pathogenesis, and immune response following infection. Therefore, this review aims to summarize advances in PCV3-related research about genomic characteristics epidemiology, pathogenesis, immune response, and the development of diagnostics. PCV3 has been detected globally and retrospectively in pigs of all ages and is associated with a range of clinical presentations, including multisystemic inflammatory syndrome, reproductive failure, porcine dermatitis and nephropathy syndrome, and subclinical infection. Experimental studies have successfully reproduced multisystemic inflammation but have not detected clinical disease. These findings, coupled with a large number of reports of coinfections coinciding with PCV3, may suggest that PCV3 infection alone may not be sufficient to cause evidenceable clinical disease. The pathogenesis of PCV3 has not been fully elucidated yet, and while receptors that facilitate cell-viral entry have not been identified, replication has been confirmed in a wide range of cell types, including trophoblasts, myocardiocytes, skin adipocytes, and neurons. PCV3 seems to evade the host immune response as evidenced by persistent viremia 42 days post-infection in experimental and longitudinal field studies despite a strong humoral response. Minimal differences in host cytokine profiles and peripheral cell-mediated responses have been observed, but certainly many questions still surround the mechanisms by which PCV3 evades the immune response. The epidemiology of PCV3 remains unclear, and the exact routes of transmission have not been described; but, PCV3 can be shed in oral fluids, nasal secretions, feces, colostrum, and semen, demonstrating the importance of lateral and vertical transmission. The detection of PCV3 in numerous domesticated and wild animal species, including cattle, dogs, mice, wild boar, chamois, roe deer, ticks, and mosquitoes, suggests the potential for multiple reservoirs and cross-species transmission. Current advances in PCV3 diagnostic tests have the ability to differentiate PCV3 from other PCVs and corroborate its presence within lesions. Given that the economic impact associated with PCV3 infection has not been assessed and the virus has the potential to emerge as a high-prevalence pathogen in the coming years, future research should focus on filling the knowledge gaps identified in this review.
    MeSH term(s) Animals ; Circoviridae Infections/diagnosis ; Circoviridae Infections/epidemiology ; Circoviridae Infections/veterinary ; Circovirus ; Immune System Diseases ; Mice ; Phylogeny ; Retrospective Studies ; Swine ; Swine Diseases/diagnosis ; Swine Diseases/epidemiology ; Swine Diseases/pathology
    Language English
    Publishing date 2022-04-01
    Publishing country Netherlands
    Document type Journal Article ; Review
    ZDB-ID 605780-9
    ISSN 1872-7492 ; 0168-1702
    ISSN (online) 1872-7492
    ISSN 0168-1702
    DOI 10.1016/j.virusres.2022.198764
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Spillover of Canine Parvovirus Type 2 to Pigs, South Dakota, USA, 2020.

    Temeeyasen, Gun / Sharafeldin, Tamer A / Lin, Chun-Ming / Hause, Ben M

    Emerging infectious diseases

    2022  Volume 28, Issue 2, Page(s) 471–473

    Abstract: In 1978, canine parvovirus type 2 originated from spillover of a feline panleukopenia-like virus, causing a worldwide pandemic of enteritis and myocarditis among canids. In 2020, the virus was identified in pigs in South Dakota, USA, by PCR, sequencing, ... ...

    Abstract In 1978, canine parvovirus type 2 originated from spillover of a feline panleukopenia-like virus, causing a worldwide pandemic of enteritis and myocarditis among canids. In 2020, the virus was identified in pigs in South Dakota, USA, by PCR, sequencing, in situ hybridization, and serology. Genetic analysis suggests spillover from wildlife.
    MeSH term(s) Animals ; Animals, Wild ; Cats ; Dogs ; Feline Panleukopenia ; Feline Panleukopenia Virus/genetics ; Parvoviridae Infections/epidemiology ; Parvoviridae Infections/veterinary ; Parvovirus, Canine/genetics ; South Dakota/epidemiology ; Swine
    Language English
    Publishing date 2022-02-20
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1380686-5
    ISSN 1080-6059 ; 1080-6040
    ISSN (online) 1080-6059
    ISSN 1080-6040
    DOI 10.3201/eid2802.211681
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Comprehensive review on immunopathogenesis, diagnostic and epidemiology of Senecavirus A

    Houston, Elizabeth / Temeeyasen, Gun / Piñeyro, Pablo Enrique

    Virus research. 2020 Sept., v. 286

    2020  

    Abstract: Senecavirus A (SVA), formerly known as Seneca Valley virus, is a single-strand, positive-sense RNA virus in the family Picornaviridae. This virus has been associated with recent outbreaks of vesicular disease (SVA-VD) and epidemic transient neonatal ... ...

    Abstract Senecavirus A (SVA), formerly known as Seneca Valley virus, is a single-strand, positive-sense RNA virus in the family Picornaviridae. This virus has been associated with recent outbreaks of vesicular disease (SVA-VD) and epidemic transient neonatal losses (ETNL) in several swine-producing countries. The clinical manifestation of and lesion caused by SVA are indistinguishable from other vesicular diseases. Pathogenicity studies indicate that SVA could regulate the host innate immune response to facilitate virus replication and the spread of the virus to bystander cells. SVA infection can induce specific humoral and cellular responses that can be detected within the first week of infection. However, SVA seems to produce persistent infection, and the virus can be shed in oral fluids for a month and detected in tissues for approximately two months after experimental infection. SVA transmission could be horizontal or vertical in infected herds of swine, while positive animals can also remain subclinical. In addition, mice seem to act as reservoirs, and the virus can persist in feed and feed ingredients, increasing the risk of introduction into naïve farms. Besides the pathological effects in swine, SVA possesses cytolytic activity, especially in neoplastic cells. Thus, SVA has been evaluated in phase II clinical trials as a virotherapy for neuroendocrine tumors. The goal of this review is summarize the current SVA-related research in pathogenesis, immunity, epidemiology and advances in diagnosis as well as discuses current challenges with subclinical/persistent presentation.
    Keywords Senecavirus A ; chronic diseases ; clinical trials ; farms ; herds ; ingredients ; innate immunity ; mice ; neoplasm cells ; neoplasms ; pathogenesis ; pathogenicity ; risk ; swine ; tissues ; virus replication ; viruses
    Language English
    Dates of publication 2020-09
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 605780-9
    ISSN 1872-7492 ; 0168-1702
    ISSN (online) 1872-7492
    ISSN 0168-1702
    DOI 10.1016/j.virusres.2020.198038
    Database NAL-Catalogue (AGRICOLA)

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  10. Article ; Online: Comprehensive review on immunopathogenesis, diagnostic and epidemiology of Senecavirus A.

    Houston, Elizabeth / Temeeyasen, Gun / Piñeyro, Pablo Enrique

    Virus research

    2020  Volume 286, Page(s) 198038

    Abstract: Senecavirus A (SVA), formerly known as Seneca Valley virus, is a single-strand, positive-sense RNA virus in the family Picornaviridae. This virus has been associated with recent outbreaks of vesicular disease (SVA-VD) and epidemic transient neonatal ... ...

    Abstract Senecavirus A (SVA), formerly known as Seneca Valley virus, is a single-strand, positive-sense RNA virus in the family Picornaviridae. This virus has been associated with recent outbreaks of vesicular disease (SVA-VD) and epidemic transient neonatal losses (ETNL) in several swine-producing countries. The clinical manifestation of and lesion caused by SVA are indistinguishable from other vesicular diseases. Pathogenicity studies indicate that SVA could regulate the host innate immune response to facilitate virus replication and the spread of the virus to bystander cells. SVA infection can induce specific humoral and cellular responses that can be detected within the first week of infection. However, SVA seems to produce persistent infection, and the virus can be shed in oral fluids for a month and detected in tissues for approximately two months after experimental infection. SVA transmission could be horizontal or vertical in infected herds of swine, while positive animals can also remain subclinical. In addition, mice seem to act as reservoirs, and the virus can persist in feed and feed ingredients, increasing the risk of introduction into naïve farms. Besides the pathological effects in swine, SVA possesses cytolytic activity, especially in neoplastic cells. Thus, SVA has been evaluated in phase II clinical trials as a virotherapy for neuroendocrine tumors. The goal of this review is summarize the current SVA-related research in pathogenesis, immunity, epidemiology and advances in diagnosis as well as discuses current challenges with subclinical/persistent presentation.
    MeSH term(s) Animals ; Disease Outbreaks ; Mice ; Picornaviridae/immunology ; Picornaviridae/pathogenicity ; Picornaviridae Infections/diagnosis ; Picornaviridae Infections/epidemiology ; Picornaviridae Infections/veterinary ; Swine ; Swine Diseases/diagnosis ; Swine Diseases/epidemiology ; Swine Diseases/virology
    Language English
    Publishing date 2020-05-30
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 605780-9
    ISSN 1872-7492 ; 0168-1702
    ISSN (online) 1872-7492
    ISSN 0168-1702
    DOI 10.1016/j.virusres.2020.198038
    Database MEDical Literature Analysis and Retrieval System OnLINE

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