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  1. Article: Modelling the tumor immune microenvironment for precision immunotherapy.

    Mackenzie, Nathan J / Nicholls, Clarissa / Templeton, Abby R / Perera, Mahasha Pj / Jeffery, Penny L / Zimmermann, Kate / Kulasinghe, Arutha / Kenna, Tony J / Vela, Ian / Williams, Elizabeth D / Thomas, Patrick B

    Clinical & translational immunology

    2022  Volume 11, Issue 6, Page(s) e1400

    Abstract: The complexity of the cellular and acellular players within the tumor microenvironment (TME) allows for significant variation in TME constitution and role in anticancer treatment response. Spatial alterations in populations of tumor cells and adjacent ... ...

    Abstract The complexity of the cellular and acellular players within the tumor microenvironment (TME) allows for significant variation in TME constitution and role in anticancer treatment response. Spatial alterations in populations of tumor cells and adjacent non-malignant cells, including endothelial cells, fibroblasts and tissue-infiltrating immune cells, often have a major role in determining disease progression and treatment response in cancer. Many current standard systemic antineoplastic treatments target the cancer cells and could be further refined to directly target commonly dysregulated cell populations of the TME. Recent developments in immuno-oncology and bioengineering have created an attractive potential to model these complexities at the level of the individual patient. These developments, along with the increasing momentum in precision medicine research and application, have catalysed exciting new discoveries in understanding drug-TME interactions, target identification, and improved efficacy of therapies. While rapid progress has been made, there are still many challenges to overcome in the development of accurate
    Language English
    Publishing date 2022-06-26
    Publishing country Australia
    Document type Journal Article ; Review
    ZDB-ID 2694482-0
    ISSN 2050-0068
    ISSN 2050-0068
    DOI 10.1002/cti2.1400
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Patient-Derived Explants as a Precision Medicine Patient-Proximal Testing Platform Informing Cancer Management.

    Templeton, Abby R / Jeffery, Penny L / Thomas, Patrick B / Perera, Mahasha P J / Ng, Gary / Calabrese, Alivia R / Nicholls, Clarissa / Mackenzie, Nathan J / Wood, Jack / Bray, Laura J / Vela, Ian / Thompson, Erik W / Williams, Elizabeth D

    Frontiers in oncology

    2021  Volume 11, Page(s) 767697

    Abstract: Precision medicine approaches that inform clinical management of individuals with cancer are progressively advancing. Patient-derived explants (PDEs) provide a patient- ... ...

    Abstract Precision medicine approaches that inform clinical management of individuals with cancer are progressively advancing. Patient-derived explants (PDEs) provide a patient-proximal
    Language English
    Publishing date 2021-12-20
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2649216-7
    ISSN 2234-943X
    ISSN 2234-943X
    DOI 10.3389/fonc.2021.767697
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Culture of bladder cancer organoids as precision medicine tools

    Thomas, Patrick B. / Perera, Mahasha P. J. / Alinezhad, Saeid / Joshi, Andre / Saadat, Paria / Nicholls, Clarissa / Devonport, Caitlin P. / Calabrese, Alivia R. / Templeton, Abby R. / Wood, Jack R. / Mackenzie, Nathan J. / Jeffery, Penny L. / Vela, Ian / Williams, Elizabeth D.

    Journal of visualized experiments. 2021 Dec. 28, , no. 178

    2021  

    Abstract: Current in vitro therapeutic testing platforms lack relevance to tumor pathophysiology, typically employing cancer cell lines established as two-dimensional (2D) cultures on tissue culture plastic. There is a critical need for more representative models ... ...

    Abstract Current in vitro therapeutic testing platforms lack relevance to tumor pathophysiology, typically employing cancer cell lines established as two-dimensional (2D) cultures on tissue culture plastic. There is a critical need for more representative models of tumor complexity that can accurately predict therapeutic response and sensitivity. The development of three-dimensional (3D) ex vivo culture of patient-derived organoids (PDOs), derived from fresh tumor tissues, aims to address these shortcomings. Organoid cultures can be used as tumor surrogates in parallel to routine clinical management to inform therapeutic decisions by identifying potential effective interventions and indicating therapies that may be futile. Here, this procedure aims to describe strategies and a detailed step-by-step protocol to establish bladder cancer PDOs from fresh, viable clinical tissue. Our well-established, optimized protocols are practical to set up 3D cultures for experiments using limited and diverse starting material directly from patients or patient-derived xenograft (PDX) tumor material. This procedure can also be employed by most laboratories equipped with standard tissue culture equipment. The organoids generated using this protocol can be used as ex vivo surrogates to understand both the molecular mechanisms underpinning urological cancer pathology and to evaluate treatments to inform clinical management.
    Keywords equipment ; neoplasm cells ; organoids ; pathophysiology ; plastics ; precision medicine ; protocols ; tissue culture ; urinary bladder neoplasms ; xenotransplantation
    Language English
    Dates of publication 2021-1228
    Size p. e63192.
    Publishing place Journal of Visualized Experiments
    Document type Article
    ZDB-ID 2259946-0
    ISSN 1940-087X
    ISSN 1940-087X
    DOI 10.3791/63192
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Culture of Bladder Cancer Organoids as Precision Medicine Tools.

    Thomas, Patrick B / Perera, Mahasha P J / Alinezhad, Saeid / Joshi, Andre / Saadat, Paria / Nicholls, Clarissa / Devonport, Caitlin P / Calabrese, Alivia R / Templeton, Abby R / Wood, Jack R / Mackenzie, Nathan J / Jeffery, Penny L / Vela, Ian / Williams, Elizabeth D

    Journal of visualized experiments : JoVE

    2021  , Issue 178

    Abstract: Current in vitro therapeutic testing platforms lack relevance to tumor pathophysiology, typically employing cancer cell lines established as two-dimensional (2D) cultures on tissue culture plastic. There is a critical need for more representative models ... ...

    Abstract Current in vitro therapeutic testing platforms lack relevance to tumor pathophysiology, typically employing cancer cell lines established as two-dimensional (2D) cultures on tissue culture plastic. There is a critical need for more representative models of tumor complexity that can accurately predict therapeutic response and sensitivity. The development of three-dimensional (3D) ex vivo culture of patient-derived organoids (PDOs), derived from fresh tumor tissues, aims to address these shortcomings. Organoid cultures can be used as tumor surrogates in parallel to routine clinical management to inform therapeutic decisions by identifying potential effective interventions and indicating therapies that may be futile. Here, this procedure aims to describe strategies and a detailed step-by-step protocol to establish bladder cancer PDOs from fresh, viable clinical tissue. Our well-established, optimized protocols are practical to set up 3D cultures for experiments using limited and diverse starting material directly from patients or patient-derived xenograft (PDX) tumor material. This procedure can also be employed by most laboratories equipped with standard tissue culture equipment. The organoids generated using this protocol can be used as ex vivo surrogates to understand both the molecular mechanisms underpinning urological cancer pathology and to evaluate treatments to inform clinical management.
    MeSH term(s) Humans ; Organoids/pathology ; Precision Medicine ; Urinary Bladder Neoplasms/pathology ; Urologic Neoplasms/pathology
    Language English
    Publishing date 2021-12-28
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Video-Audio Media
    ZDB-ID 2259946-0
    ISSN 1940-087X ; 1940-087X
    ISSN (online) 1940-087X
    ISSN 1940-087X
    DOI 10.3791/63192
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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