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Article ; Online: Single-cell RNA-sequencing in asthma research.

Tang, Weifeng / Li, Mihui / Teng, Fangzhou / Cui, Jie / Dong, Jingcheng / Wang, Wenqian

2022 Volume 13, Page(s) 988573

Abstract: Asthma is a complex and heterogeneous disease with multicellular involvement, and knowledge gaps remain in our understanding of the pathogenesis of asthma. Efforts are still being made to investigate the immune pathogenesis of asthma in order to identify ...

Abstract	Asthma is a complex and heterogeneous disease with multicellular involvement, and knowledge gaps remain in our understanding of the pathogenesis of asthma. Efforts are still being made to investigate the immune pathogenesis of asthma in order to identify possible targets for prevention. Single cell RNA sequencing (scRNA-seq) technology is a useful tool for exploring heterogeneous diseases, identifying rare cell types and distinct cell subsets, enabling elucidation of key processes of cell differentiation, and understanding regulatory gene networks that predict immune function. In this article, we provide an overview of the importance of scRNA-seq for asthma research, followed by an in-depth discussion of the results in recent years, in order to provide new ideas for the pathogenesis, drug development and treatment of asthma.
Language	English
Publishing date	2022-11-29
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2022.988573
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Active ingredients from Chinese medicine plants as therapeutic strategies for asthma: Overview and challenges.

Wang, Wenqian / Yao, Qiang / Teng, Fangzhou / Cui, Jie / Dong, Jingcheng / Wei, Ying

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

2021 Volume 137, Page(s) 111383

Abstract: Although considerable advance has been made in diagnosing and treating, asthma is still a serious public health challenge. Traditional Chinese medicine (TCM) is an effective therapy of complementary and alternative medicine. More and more scientific ... ...

Abstract	Although considerable advance has been made in diagnosing and treating, asthma is still a serious public health challenge. Traditional Chinese medicine (TCM) is an effective therapy of complementary and alternative medicine. More and more scientific evidences support the use of TCM for asthma treatment, and active ingredients from Chinese medicine plants are becoming a hot issue. Purpose of review: To summarize the frontier knowledge on the function and underlying mechanisms of the active ingredients in asthma treatments and provide a fully integrated, reliable reference for exploring innovative treatments for asthma. Methods: The cited literature was obtained from the PubMed and CNIK databases (up to September 2020). Experimental studies on the active ingredients of Chinese medicine and their therapeutic mechanisms were identified. The key words used in the literature retrieval were "asthma" and "traditional Chinese medicine" or "Chinese herbal medicine". The literature on the active ingredients was then screened manually. Results: We summarized the effect of these active ingredients on asthma, primarily including the effect through which these ingredients can regulate the immunologic equilibrium mechanism by acting on a number of signalling pathways, such as Notch, JAK-STAT-MAPK, adiponectin-iNOS-NF-κB, PGD2-CRTH2, PI3K/AKT, Keap1-Nrf2/HO-1, T-bet/Gata-3 and Foxp3-RORγt, thereby regulating the progression of asthma. Conclusion: The active ingredients from Chinese medicine have multilevel effects on asthma by regulating the immunologic equilibrium mechanism or signalling pathways, giving them great clinical value. However, the safety and functional mechanism of these ingredients still must be further determined.
MeSH term(s)	Animals ; Anti-Asthmatic Agents/chemistry ; Anti-Asthmatic Agents/pharmacology ; Asthma/drug therapy ; Drugs, Chinese Herbal/chemistry ; Humans ; Medicine, Chinese Traditional ; Plants, Medicinal/chemistry
Chemical Substances	Anti-Asthmatic Agents ; Drugs, Chinese Herbal
Language	English
Publishing date	2021-02-16
Publishing country	France
Document type	Journal Article ; Review
ZDB-ID	392415-4
ISSN	1950-6007 ; 0753-3322 ; 0300-0893
ISSN (online)	1950-6007
ISSN	0753-3322 ; 0300-0893
DOI	10.1016/j.biopha.2021.111383
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Article: Screening and Verification of Differentially Expressed Long Non-coding RNAs in the Peripheral Blood of Patients With asthma.

Ma, Cheng / Wang, Shiyuan / Cao, Yuxue / Tang, Weifeng / Wuniqiemu, Tulake / Teng, Fangzhou / Zhu, Xueyi / Wei, Ying / Dong, Jingcheng

Frontiers in pharmacology

2022 Volume 13, Page(s) 834009

Abstract: Growing evidence suggests that long non-coding RNAs (lncRNAs) play a key role in the pathogenesis of asthma. Although some differentially expressed lncRNAs have been identified in asthmatic patients, many asthma-related lncRNAs have not been annotated. ... ...

Abstract	Growing evidence suggests that long non-coding RNAs (lncRNAs) play a key role in the pathogenesis of asthma. Although some differentially expressed lncRNAs have been identified in asthmatic patients, many asthma-related lncRNAs have not been annotated. In the present study, six patients and three healthy subjects were randomly selected from 34 asthmatic patients and 17 healthy subjects. Second-generation high-throughput sequencing was performed on their peripheral blood samples. There were 1,137 differentially expressed lncRNAs in the asthma patients compared to in the healthy controls, of which 485 were upregulated and 652 were downregulated. The top 30 enriched GO and KEGG terms were identified, and the cytosolic ribosome (GO:0022626) and ribosome (hsa03010) were associated with the most differentially expressed lncRNAs. The top 10 differentially expressed lncRNAs associated with asthma were verified by an lncRNA-mRNA co-expression network and RT-qPCR. Seven of the these (NONHSAT015495.2, MSTRG.71212.2, NONHSAT163272.1, NONHSAT181891.1, NONHSAT190964.1, ENST00000564809, and NONHSAT076890.2) were down-regulated in the peripheral blood of asthmatic patients, which was consistent with the sequencing results. Three patients and three healthy subjects were randomly selected from the remaining subjects to verify these seven lncRNAs by RT-qPCR, which further confirmed the sequencing results. Public database GSE106230 was also in agreement with the FPKM (Fragments Per kilobase of exon model per Million mapped reads) trends of ENST00000564809, NONHSAT015495.2, NONHSAT181891.1, and NONHSAT190964.1. In conclusion, the present study identified seven lncRNAs that may serve as potential biological markers for asthma.
Language	English
Publishing date	2022-02-22
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2022.834009
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Article ; Online: Ferroptosis, novel therapeutics in asthma.

Lv, Xiaodi / Dong, Ming / Tang, Weifeng / Qin, Jingjing / Wang, Wenqian / Li, Mihui / Teng, Fangzhou / Yi, La / Dong, Jingcheng / Wei, Ying

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

2022 Volume 153, Page(s) 113516

Abstract: Ferroptosis, an iron-dependent form of regulated cell death, was recently demonstrated to be closely associated with the immune system. Regulators of ferroptosis may be the cells and secretions of the immune system. Ferroptosis has contributed to the ... ...

Abstract	Ferroptosis, an iron-dependent form of regulated cell death, was recently demonstrated to be closely associated with the immune system. Regulators of ferroptosis may be the cells and secretions of the immune system. Ferroptosis has contributed to the progression of various diseases, namely, cancer, ischemia, and degenerative diseases. However, research on the relationship between ferroptosis and asthma remains fragmented. Non-immune cells associated with asthma are also closely associated with ferroptosis. Further studies on cross-linking asthma inflammation with ferroptosis signaling pathways could help identify several key molecules, known as ferroptosis regulators, that regulate asthma. Ferroptosis provides a new perspective to interpret and understand the manifestations of asthma, potential drug discovery targets, and new therapeutic options to effectively intervene in the imbalance caused by abnormal inflammation in asthma. Thus, the pathogenesis of ferroptosis and its contribution to the pathogenesis of asthma is essential in deepening the understanding and improving the prognosis and cure rate of the patients. Herein, we introduce the main molecular mechanisms of ferroptosis and asthma, describe the relationship between ferroptosis and asthma based on their common regulatory cells or molecules, and discuss potential drug discovery targets and therapeutic applications of ferroptosis in the context of immunomodulation and symptom alleviation.
MeSH term(s)	Asthma/drug therapy ; Ferroptosis ; Humans ; Inflammation/drug therapy ; Iron/metabolism ; Signal Transduction
Chemical Substances	Iron (E1UOL152H7)
Language	English
Publishing date	2022-08-06
Publishing country	France
Document type	Journal Article ; Review
ZDB-ID	392415-4
ISSN	1950-6007 ; 0753-3322 ; 0300-0893
ISSN (online)	1950-6007
ISSN	0753-3322 ; 0300-0893
DOI	10.1016/j.biopha.2022.113516
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Article: Proteomics analysis reveals suppression of IL-17 signaling pathways contributed to the therapeutic effects of Jia-Wei Bu-Shen-Yi-Qi formula in a murine asthma model

Qin, Jingjing / Wuniqiemu, Tulake / Wei, Ying / Teng, Fangzhou / Cui, Jie / Sun, Jing / Yi, La / Tang, Weifeng / Zhu, Xueyi / Xu, Weifang / Dong, Jingcheng

Phytomedicine. 2022 Jan., v. 95

2022

Abstract: Jia-Wei Bu-Shen-Yi-Qi formula (JWBSYQF), a Chinese herbal formula, is a commonly used prescription for treating asthma patients. However, the targeted proteins associated with JWBSYQF treatment remain unknown.Present study aims to evaluate the ... ...

Abstract	Jia-Wei Bu-Shen-Yi-Qi formula (JWBSYQF), a Chinese herbal formula, is a commonly used prescription for treating asthma patients. However, the targeted proteins associated with JWBSYQF treatment remain unknown.Present study aims to evaluate the therapeutic efficacy of JWBSYQF and identify the targeted proteins in addition to functional pathways.The ovalbumin (OVA)-induced murine asthma model was established to explore the therapeutic effect of JWBSYQF treatment. Proteomic profiling and quantifications were performed using data-independent acquisition (DIA) methods. Differentially expressed proteins (DEPs) were validated via western blot (WB) and immunohistochemistry (IHC).A murine asthma model was made by OVA sensitization and challenge, and JWBSYQF (2.25, 4.50, 9,00 g/kg body weight) or dexamethasone (1 mg/ kg body weight) were administered orally. Airway hyperresponsiveness (AHR) to methacholine (Mch), inflammatory cell counts and classification in bronchoalveolar lavage fluid (BALF), lung histopathology, and cytokine levels were measured. Furthermore, DIA proteomic analyses were performed to explore the DEPs targeted by JWBSYQF and were further validated by WB and IHC.Our results exhibited that JWBSYQF attenuated AHR which was mirrored by decreased airway resistance and increased lung compliance. In addition, JWBSYQF-treated mice showed reduced inflammatory score, mucus hypersecretion, as well as reduced the number of BALF leukocytes along with decreased content of BALF Th2 inflammatory cytokines (IL-4, IL-5, IL-13) and serum IgE. Proteomics analysis identified 704 DEPs between the asthmatic mice and control group (MOD vs CON), and 120 DEPs between the JWBSYQF-treatment and the asthmatic mice (JWB-M vs MOD). A total of 33 overlapped DEPs were identified among the three groups. Pathway enrichment analysis showed that DEPs were significantly enriched in IL-17 signaling pathway, in which DEPs, Lcn2, TGF-β1, Gngt2, and Ppp2r5e were common DEPs between three experimental groups. WB and IHC results further validated expressional levels and tendency of these proteins. Our results also showed that JWBSYQF affects mitogen activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) signaling pathways, that are activated by IL-17 signaling.The present study suggested that JWBSYQF could attenuate AHR and airway inflammation in OVA-induced asthmatic mice. In addition, proteomics analysis revealed that suppression of IL-17 signaling pathways contributes to the therapeutic effects of JWBSYQF.
Keywords	Western blotting ; animal disease models ; asthma ; blood serum ; body weight ; dexamethasone ; gene expression regulation ; histopathology ; hypersecretion ; immunohistochemistry ; inflammation ; interleukin-13 ; interleukin-17 ; interleukin-4 ; interleukin-5 ; lung function ; lungs ; mitogen-activated protein kinase ; mucus ; ovalbumin ; proteomics ; therapeutics
Language	English
Dates of publication	2022-01
Publishing place	Elsevier GmbH
Document type	Article
ZDB-ID	1205240-1
ISSN	1618-095X ; 0944-7113
ISSN (online)	1618-095X
ISSN	0944-7113
DOI	10.1016/j.phymed.2021.153803
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Trends of therapy in the treatment of asthma.

Lv, Xiaodi / Gao, Zhen / Tang, Weifeng / Qin, Jingjing / Wang, Wenqian / Liu, Jiaqi / Li, Mihui / Teng, Fangzhou / Yi, La / Dong, Jingcheng / Wei, Ying

Therapeutic advances in respiratory disease

2023 Volume 17, Page(s) 17534666231155748

Abstract: Background: To better understand the development of therapy for asthma, grasp the core paradigm associated with the transformation of cognition of asthma treatment and asthma, explore potential and effective therapies for asthma, discover new biomarkers ...

Abstract	Background: To better understand the development of therapy for asthma, grasp the core paradigm associated with the transformation of cognition of asthma treatment and asthma, explore potential and effective therapies for asthma, discover new biomarkers and mechanisms related to asthma treatment, find novel targets for anti-asthma drugs, and predict the future trends of asthma therapy, we used a bibliometric analysis to research articles related to the therapies for asthma published from 1983 to 2022. Methods: A comprehensive search was conducted to analyze the articles associated with therapy for asthma with the help of the Web of Science Core Collection (WOSCC) database from January 1, 1983 to August 14, 2022. The CiteSpace 6.1.R2 software and VOS viewer 6.1.8 software were utilized to analyze the overall structure of the network, network clusters, links between clusters, key nodes, and pathways. Results: A total of 3902 publications related to therapies on asthma were published in 3211 academic journals by a total of 14,655 authors in 3476 organizations from 87 countries or regions from 1983 to 2022. The United States published the most articles ( Conclusions: The results from this biometric review provide insight into the development of therapy for asthma, the paradigm of recognition of this field, the approach of discovering new targets, exploration and combination of new mechanisms, and the frontier trend of this field in future.
MeSH term(s)	Humans ; Asthma/diagnosis ; Asthma/drug therapy ; China ; Databases, Factual ; England ; Inflammation ; Randomized Controlled Trials as Topic
Language	English
Publishing date	2023-03-21
Publishing country	England
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	2476459-0
ISSN	1753-4666 ; 1753-4658
ISSN (online)	1753-4666
ISSN	1753-4658
DOI	10.1177/17534666231155748
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Article ; Online: TMT-based quantitative proteomics revealed protective efficacy of Icariside II against airway inflammation and remodeling via inhibiting LAMP2, CTSD and CTSS expression in OVA-induced chronic asthma mice.

Zhou, Yaolong / Huang, Xi / Yu, Hang / Shi, Hanlin / Chen, Mengmeng / Song, Jingrong / Tang, Weifeng / Teng, Fangzhou / Li, Congcong / Yi, La / Zhu, Xueyi / Wang, Na / Wei, Ying / Wuniqiemu, Tulake / Dong, Jingcheng

Phytomedicine : international journal of phytotherapy and phytopharmacology

2023 Volume 118, Page(s) 154941

Abstract: Background: Asthma is a chronic inflammatory disorder in airways with typical pathologic features of airflow limitation, airway inflammation and remodeling. Icariside II (IS), derived from herbal medicine Herba Epimedii, exerts an anti-inflammatory ... ...

Abstract	Background: Asthma is a chronic inflammatory disorder in airways with typical pathologic features of airflow limitation, airway inflammation and remodeling. Icariside II (IS), derived from herbal medicine Herba Epimedii, exerts an anti-inflammatory property. However, underlying mechanisms with specifically targeted molecular expression by IS in asthma have not been fully understood, and whether IS could inhibit remodeling and EMT still remains unclear. Purpose: The study aimed to clarify therapeutic efficacy of IS for attenuating airway inflammation and remodeling in asthma, and illustrate IS-regulated specific pathway and target proteins through TMT-based quantitative proteomics. Study design and methods: Murine model of chronic asthma was constructed with ovalbumin (OVA) sensitization and then challenge for 8 weeks. Pulmonary function, leukocyte count in bronchoalveolar lavage fluid (BALF), lung histopathology, inflammatory and fibrotic cytokines, and markers of epithelial-mesenchymal transition (EMT) were evaluated. TMT-based quantitative proteomics were performed on lung tissues to explore IS-regulated proteins. Results: IS contributed to alleviative airway hyperresponsiveness (AHR) evidenced by declined R Conclusions: The study demonstrated IS could ameliorate AHR, airway inflammation, remodeling and EMT in OVA-induced chronic asthma mice. Our research was the first to reveal that inhibition of LAMP2, CTSD and CTSS expression in autophagy contributed to the therapeutic efficacy of IS to asthma.
MeSH term(s)	Mice ; Animals ; Ovalbumin ; Proteomics ; Asthma/drug therapy ; Asthma/metabolism ; Lung/pathology ; Inflammation/metabolism ; Bronchoalveolar Lavage Fluid ; Cytokines ; Disease Models, Animal ; Mice, Inbred BALB C
Chemical Substances	Ovalbumin (9006-59-1) ; baohuoside I (113558-15-9) ; Cytokines
Language	English
Publishing date	2023-06-25
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1205240-1
ISSN	1618-095X ; 0944-7113
ISSN (online)	1618-095X
ISSN	0944-7113
DOI	10.1016/j.phymed.2023.154941
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Article ; Online: Regulation of ferroptosis and ACSL4-15LO1 pathway contributed to the anti-asthma effect of acupuncture.

Tang, Weifeng / Qin, Jingjing / Zhou, Yaolong / Wang, Wenqian / Teng, Fangzhou / Liu, Jiaqi / Yi, La / Cui, Jie / Zhu, Xueyi / Wang, Shiyuan / Dong, Jingcheng / Wei, Ying

International immunopharmacology

2023 Volume 115, Page(s) 109670

Abstract: Acupuncture has been frequently used in China for the treatment asthma for thousands of years. Ferroptosis was recently revealed to be involved in several pathological conditions including asthma. However, the detailed links between ferroptosis and ... ...

Abstract	Acupuncture has been frequently used in China for the treatment asthma for thousands of years. Ferroptosis was recently revealed to be involved in several pathological conditions including asthma. However, the detailed links between ferroptosis and airway inflammation in asthma, as well as the detailed regulation of acupuncture on these disorders remains unclear. Our results demonstrated that the non-haem Fe
MeSH term(s)	Animals ; Mice ; Acupuncture Therapy ; Anti-Asthmatic Agents/therapeutic use ; Anti-Asthmatic Agents/pharmacology ; Asthma/therapy ; Asthma/drug therapy ; Coenzyme A Ligases/metabolism ; Coenzyme A Ligases/pharmacology ; Disease Models, Animal ; Ferroptosis ; Glutathione Disulfide/adverse effects ; Inflammation ; Interleukin-4/pharmacology ; Ovalbumin/therapeutic use ; Pneumonia/drug therapy ; Arachidonate 15-Lipoxygenase/metabolism
Chemical Substances	Acsl4 protein, mouse (EC 6.2.1.-) ; Anti-Asthmatic Agents ; Coenzyme A Ligases (EC 6.2.1.-) ; Glutathione Disulfide (ULW86O013H) ; Interleukin-4 (207137-56-2) ; Ovalbumin (9006-59-1) ; Arachidonate 15-Lipoxygenase (EC 1.13.11.33)
Language	English
Publishing date	2023-01-04
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2043785-7
ISSN	1878-1705 ; 1567-5769
ISSN (online)	1878-1705
ISSN	1567-5769
DOI	10.1016/j.intimp.2022.109670
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Article ; Online: TMT-based quantitative proteomics revealed protective efficacy of Icariside II against airway inflammation and remodeling via inhibiting LAMP2, CTSD and CTSS expression in OVA-induced chronic asthma mice

Phytomedicine. 2023 Sept., v. 118 p.154941-

2023

Abstract: Asthma is a chronic inflammatory disorder in airways with typical pathologic features of airflow limitation, airway inflammation and remodeling. Icariside II (IS), derived from herbal medicine Herba Epimedii, exerts an anti-inflammatory property. However, ...

Abstract	Asthma is a chronic inflammatory disorder in airways with typical pathologic features of airflow limitation, airway inflammation and remodeling. Icariside II (IS), derived from herbal medicine Herba Epimedii, exerts an anti-inflammatory property. However, underlying mechanisms with specifically targeted molecular expression by IS in asthma have not been fully understood, and whether IS could inhibit remodeling and EMT still remains unclear. The study aimed to clarify therapeutic efficacy of IS for attenuating airway inflammation and remodeling in asthma, and illustrate IS-regulated specific pathway and target proteins through TMT-based quantitative proteomics. Murine model of chronic asthma was constructed with ovalbumin (OVA) sensitization and then challenge for 8 weeks. Pulmonary function, leukocyte count in bronchoalveolar lavage fluid (BALF), lung histopathology, inflammatory and fibrotic cytokines, and markers of epithelial-mesenchymal transition (EMT) were evaluated. TMT-based quantitative proteomics were performed on lung tissues to explore IS-regulated proteins. IS contributed to alleviative airway hyperresponsiveness (AHR) evidenced by declined RL and increased Cdyn. After IS treatment, we observed a remarked down-regulation of leukocyte count, inflammatory cytokines in BALF, and peribronchial inflammation infiltration. Goblet cell hyperplasia, mucus secretion and peribronchial collagen deposition were attenuated, with the level of TGF-β and MMP-9 in BALF declined. Furthermore, IS induced a rise of Occludin and E-cadherin and a decline of N-cadherin and α-SMA in lung tissues. These results proved the protective property of IS against airway inflammation, remodeling and EMT. To further investigate underlying mechanisms of IS in asthma treatment, TMT-based quantitative proteomics were performed and 102 overlapped DEPs regulated by IS were identified. KEGG enrichment exhibited these DEPs were enriched in lysosome, phagosome and autophagy, in which LAMP2, CTSD and CTSS were common DEPs. WB, q-PCR and IHC results proofed expressional alteration of these proteins. Besides, IS could decrease Beclin-1 and LC3B expression with increasing p62 expression thus inhibiting autophagy. The study demonstrated IS could ameliorate AHR, airway inflammation, remodeling and EMT in OVA-induced chronic asthma mice. Our research was the first to reveal that inhibition of LAMP2, CTSD and CTSS expression in autophagy contributed to the therapeutic efficacy of IS to asthma.
Keywords	air flow ; animal models ; anti-inflammatory activity ; asthma ; autophagy ; cadherins ; collagen ; cytokines ; herbal medicines ; histopathology ; hyperplasia ; inflammation ; leukocyte count ; lung function ; lungs ; lysosomes ; mucus ; occludins ; ovalbumin ; phagosomes ; proteomics ; secretion ; therapeutics ; Icariside II ; Quantitative proteomics ; Airway inflammation ; Airway remodeling ; EMT ; AHR ; AOD ; AOI ; BALF ; BSYQF ; Cdyn ; CTSD ; CTSS ; DEPs ; ECM ; EPO ; GO ; H&E ; IL ; IOD ; IS ; KEGG ; LAMP1 ; LAMP2 ; LC-MS ; MBP ; Mch ; MMP-9 ; NF-κB ; OVA ; PAS ; RL ; RP-HPLC ; TGF-β1 ; TMT
Language	English
Dates of publication	2023-09
Publishing place	Elsevier GmbH
Document type	Article ; Online
ZDB-ID	1205240-1
ISSN	1618-095X ; 0944-7113
ISSN (online)	1618-095X
ISSN	0944-7113
DOI	10.1016/j.phymed.2023.154941
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Luteolin inhibits autophagy in allergic asthma by activating PI3K/Akt/mTOR signaling and inhibiting Beclin-1-PI3KC3 complex.

Wang, Shiyuan / Wuniqiemu, Tulake / Tang, Weifeng / Teng, Fangzhou / Bian, Qin / Yi, La / Qin, Jingjing / Zhu, Xueyi / Wei, Ying / Dong, Jingcheng

International immunopharmacology

2021 Volume 94, Page(s) 107460

Abstract: Allergic asthma is a common chronic inflammatory disease characterized by airway inflammation, mucus hypersecretion and airway remodeling. Autophagy is a highly conserved intracellular degradation pathway in eukaryotic cells. There is growing evidence ... ...

Abstract	Allergic asthma is a common chronic inflammatory disease characterized by airway inflammation, mucus hypersecretion and airway remodeling. Autophagy is a highly conserved intracellular degradation pathway in eukaryotic cells. There is growing evidence suggesting that dysregulation of autophagy is involved in the pathological process of asthma. Luteolin is a typical flavonoid compound with anti-inflammatory, anti-allergic and immune-enhancing functions. Previous studies have shown that luteolin can attenuate airway inflammation and hypersensitivity in asthma. However, whether luteolin can play a role in treating asthma by regulating autophagy remains unclear. The aim of the present study was to evaluate the therapeutic effect of luteolin on ovalbumin (OVA)-induced asthmatic mice, observe its effect on the level of autophagy in lung tissues, and further elucidate its underlying mechanism. The results showed that OVA-induced mice developed airway hyperresponsiveness, mucus over-production and collagen deposition. The number of inflammatory cells, levels of interleukin (IL)-4, IL-5 and IL-13 in bronchoalveolar lavage fluid (BALF) and OVA-specific IgE in serum were significantly increased. Furthermore, the infiltration of inflammatory cells was observed along with the activation of autophagy in lung tissues. Luteolin treatment significantly inhibited the OVA-induced inflammatory responses and the level of autophagy in lung tissues as well. Moreover, luteolin activated the PI3K/Akt/mTOR pathway and inhibited the Beclin-1-PI3KC3 protein complex in lung tissues of asthmatic mice. In conclusion, this study explored the regulatory mechanism of luteolin on autophagy in allergic asthma, providing biologic evidence for its clinical application.
MeSH term(s)	Allergens ; Animals ; Anti-Asthmatic Agents/pharmacology ; Anti-Asthmatic Agents/therapeutic use ; Asthma/drug therapy ; Asthma/immunology ; Autophagy/drug effects ; Beclin-1/immunology ; Bronchoalveolar Lavage Fluid/cytology ; Bronchoalveolar Lavage Fluid/immunology ; Cell Line ; Cytokines/immunology ; Female ; Luteolin/pharmacology ; Luteolin/therapeutic use ; Mice, Inbred BALB C ; Ovalbumin ; Phosphatidylinositol 3-Kinases/immunology ; Proto-Oncogene Proteins c-akt/immunology ; Signal Transduction/drug effects ; TOR Serine-Threonine Kinases/immunology ; Mice
Chemical Substances	Allergens ; Anti-Asthmatic Agents ; Beclin-1 ; Becn1 protein, mouse ; Cytokines ; Ovalbumin (9006-59-1) ; mTOR protein, mouse (EC 2.7.1.1) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; TOR Serine-Threonine Kinases (EC 2.7.11.1) ; Luteolin (KUX1ZNC9J2)
Language	English
Publishing date	2021-02-20
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2043785-7
ISSN	1878-1705 ; 1567-5769
ISSN (online)	1878-1705
ISSN	1567-5769
DOI	10.1016/j.intimp.2021.107460
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