LIVIVO - Search results -

Search results

Result 1 - 10 of total 17

Search options

Article: [Application of near infrared spectroscopy in screening Cordyceps militaris mutation strains and optimizing their fermentation process].

Guo, Wei-liang / Zhang, Zhuo-yong / Lu, Jian-hui / Song, Jia / Di, Wang / Teng, Li-rong

Guang pu xue yu guang pu fen xi = Guang pu

2010 Volume 30, Issue 8, Page(s) 2077–2082

Abstract: To fast screen high-yield Cordyceps militaris mutations strains and optimize their fermentation process, near infrared (NIR) spectroscopy technology combined with chemometrics has been applied to establishing models for simultaneous determination of ... ...

Abstract	To fast screen high-yield Cordyceps militaris mutations strains and optimize their fermentation process, near infrared (NIR) spectroscopy technology combined with chemometrics has been applied to establishing models for simultaneous determination of adenosine, protein, polysaccharide and Cordyceps militaris acid contents in Cordyceps militaris powder samples. Fermentations were implemented in Erlenmeyer flask with 468 Cordyceps militaris mutations strains under various fermentation conditions and Cordyceps militaris powder samples were collected. Then their NIR spectra were obtained using UV-VIS-NIR spectrometer and their adenosine, protein, polysaccharide and Cordyceps militaris acid contents were determined using reference methods. Partial least squares (PLS) method was employed to model the relationships between NIR spectra and the above mentioned components' contents in Cordyceps militaris powder samples. Monte Carlo partial least square (MCPLS) was applied to identify the outliers and select suitable number of calibration samples. Moving window partial least square (MWPLS) was applied to select the characteristic wavelength of the components. The degree of the approaching (Da) was employed as criterion for selecting effective pretreatment methods investigated. The optimum models for determination of adenosine, protein, polysaccharide and Cordyceps militaris acid contents in Cordyceps militaris powder samples were obtained with the above mentioned optimization. Their correlation between actual and predictive values of calibration samples (Re) was 0.92943, 0.98479, 0.90785, and 0.85131, respectively. Their root mean square error of prediction set (RMSEP) was 0.66714, 0.02065, 0.01131, and 0.01159, respectively. The obtained results demonstrated that the fitting and the predictive accuracy were satisfactory. It is feasible to apply this method to screen the high yield Cordyceps militaris mutation strains and optimize their fermentation process.
MeSH term(s)	Adenosine ; Calibration ; Cordyceps ; Fermentation ; Least-Squares Analysis ; Mutation ; Polysaccharides ; Proteins ; Spectroscopy, Near-Infrared
Chemical Substances	Polysaccharides ; Proteins ; Adenosine (K72T3FS567)
Language	Chinese
Publishing date	2010-08
Publishing country	China
Document type	English Abstract ; Journal Article
ISSN	1000-0593
ISSN	1000-0593
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Studies on the analgesic activities of Jia-Yuan-Qing pill and its safety evaluation in mice

Tian, Ye / Teng, Li-rong / Song, Jing-jing / Meng, Qing-fan / Lu, Jia-hui / Zhang, Wei-wei / Wei, Kang / Wang, Ning / Wang, Di / Teng, Le-sheng

Protoplasma. 2014 Sept., v. 251, no. 5

2014

Abstract: The analgesic activity of Porcellio laevis Latreille, Rhizoma Corydalis, and Radix Cynanchi Paniculati have been reported in recent years. A new formula named Jia-Yuan-Qing pill (JYQP) is therefore created by combining the three herbs at 9:7:7 ratio ... ...

Abstract	The analgesic activity of Porcellio laevis Latreille, Rhizoma Corydalis, and Radix Cynanchi Paniculati have been reported in recent years. A new formula named Jia-Yuan-Qing pill (JYQP) is therefore created by combining the three herbs at 9:7:7 ratio according to traditional Chinese theories. The present study aims to evaluate the effect of JYQP as a novel painkiller in various models. Acute toxicity test was applied to evaluate the safety of JYQP. Acetic-acid-induced writhing, hot plate test, formalin test, and naloxone-pretreated writhing test were employed to elaborate the analgesic activity of JYQP and its possible mechanism. A bone cancer pain mouse model was performed to further assess the effect of JYQP in relieving cancer pain. Test on naloxone-precipitated withdrawal symptoms was conduct to examine the physical dependence of mice on JYQP. Data revealed that JYQP reduced writhing and stretching induced by acetic acid; however, this effect could not be blocked by naloxone. JYQP specifically suppressed the phase II reaction time in formalin-treated mice; meanwhile, no analgesic effect of JYQP in hot plate test was observed, indicating that JYQP exerts analgesic activity against inflammatory pain rather than neurogenic pain. Furthermore, JYQP could successfully relieve bone cancer pain in mice. No physical dependence could be observed upon long-term administration in mice. Collectively, our present results provide experimental evidence in supporting clinical use of JYQP as an effective and safe agent for pain treatment.
Keywords	Corydalis ; Porcellio ; acetic acid ; acute toxicity ; analgesic effect ; analgesics ; animal models ; formalin ; herbs ; mice ; naloxone ; pain ; toxicity testing
Language	English
Dates of publication	2014-09
Size	p. 1245-1253.
Publishing place	Springer-Verlag
Document type	Article
ZDB-ID	123809-7
ISSN	1615-6102 ; 0033-183X
ISSN (online)	1615-6102
ISSN	0033-183X
DOI	10.1007/s00709-014-0637-9
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 46/342: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Investigations on the antifatigue and antihypoxic effects of Paecilomyces hepiali extract.

Wang, Juan / Li, Lan Zhou / Liu, Yan Ge / Teng, Li Rong / Lu, Jia Hui / Xie, Jing / Hu, Wen Ji / Liu, Yan / Liu, Yang / Wang, Di / Teng, Le Sheng

Molecular medicine reports

2016 Volume 13, Issue 2, Page(s) 1861–1868

Abstract: Paecilomyces hepiali, one of the most valuable and effective Chinese medicinal herbs, possesses potential antioxidant, immunomodulatory, antitumor and anti‑inflammatory properties. The present study aimed to investigate the antifatigue and antihypoxic ... ...

Abstract	Paecilomyces hepiali, one of the most valuable and effective Chinese medicinal herbs, possesses potential antioxidant, immunomodulatory, antitumor and anti‑inflammatory properties. The present study aimed to investigate the antifatigue and antihypoxic effects of Paecilomyces hepiali extract (PHC) in a mouse model. Using a rotating rod, forced swimming and running assessment, the antifatigue activity of PHC was determined. PHC administration for 7 days had no effect on mouse horizontal or vertical movement, indicating no neurotoxicity at the selected doses was observed. Using a normobaric hypoxia, sodium nitrite toxicosis and acute cerebral ischemia assessments, PHC was confirmed to possess antihypoxic effects. PHC treatment for 7 days significantly enhanced the serum and liver levels of adenosine triphosphate, superoxide dismutase and glutathione peroxidase, prior to and following 60 min of swimming. The levels of antioxidant‑associated proteins in the livers of the mice were analyzed using western blotting. PHC effectively increased the expression levels of phosphorylated (p)‑5'‑monophosphate (AMP)‑activated protein kinase (AMPK), p‑protein kinase B (AKT) and p‑mammalian target of rapamycin (mTOR). The results of the present study demonstrated that PHC efficiently enhanced endurance from fatigue and had antihypoxic effects through elevation of the antioxidant capacity in the serum and liver, at least in part through the AMPK and AKT/mTOR pathways. These results indicate the potential of this natural product as an antioxidant in the treatment of fatigue, hypoxia and their associated diseases.
MeSH term(s)	AMP-Activated Protein Kinases/metabolism ; Adenosine Triphosphate/metabolism ; Animals ; Autonomic Nervous System/drug effects ; Complex Mixtures/pharmacology ; Complex Mixtures/therapeutic use ; Fatigue/complications ; Fatigue/drug therapy ; Fatigue/enzymology ; Female ; Glutathione Peroxidase/blood ; Hypoxia/complications ; Hypoxia/drug therapy ; Hypoxia/enzymology ; Male ; Mice ; Phosphorylation/drug effects ; Rhodiola/chemistry ; Superoxide Dismutase/blood ; Swimming ; TOR Serine-Threonine Kinases/metabolism
Chemical Substances	Complex Mixtures ; Adenosine Triphosphate (8L70Q75FXE) ; Glutathione Peroxidase (EC 1.11.1.9) ; Superoxide Dismutase (EC 1.15.1.1) ; TOR Serine-Threonine Kinases (EC 2.7.1.1) ; AMP-Activated Protein Kinases (EC 2.7.11.31)
Language	English
Publishing date	2016-02
Publishing country	Greece
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1791-3004
ISSN (online)	1791-3004
DOI	10.3892/mmr.2015.4734
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Study of the analgesic activities, chronic toxicity and addictive potential of Jia-Yuan-Qing pill in rats.

Tian, Y E / Teng, Li-Rong / Wang, Zhen-Zuo / Zhao, Min / Meng, Qing-Fan / Lu, Jia-Hui / Tian, Jian-Ming / Zhang, Wei-Wei / Zheng, Xiaoyi / Wang, D I / Teng, LE-Sheng

Experimental and therapeutic medicine

2015 Volume 9, Issue 6, Page(s) 2349–2355

Abstract: Jia-Yuan-Qing pill (JYQP) composed ... ...

Abstract	Jia-Yuan-Qing pill (JYQP) composed of
Language	English
Publishing date	2015-04-15
Publishing country	Greece
Document type	Journal Article
ZDB-ID	2683844-8
ISSN	1792-1015 ; 1792-0981
ISSN (online)	1792-1015
ISSN	1792-0981
DOI	10.3892/etm.2015.2426
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: [Application of near infrared spectroscopy in rapid and simultaneous determination of essential components in five varieties of anti-tuberculosis tablets].

Teng, Le-sheng / Wang, Di / Song, Jia / Zhang, Yi-bo / Guo, Wei-liang / Teng, Li-rong

Guang pu xue yu guang pu fen xi = Guang pu

2008 Volume 28, Issue 8, Page(s) 1814–1818

Abstract: Since 1980s, tuberculosis has become increasingly serious. Rifampicin tablets, isoniazide tablets, pyrazinamide tablets, rifampicin and isoniazide tablets and rifampicin isoniazide and pyrazinamide tablets are currently relatively efficacious ... ...

Abstract	Since 1980s, tuberculosis has become increasingly serious. Rifampicin tablets, isoniazide tablets, pyrazinamide tablets, rifampicin and isoniazide tablets and rifampicin isoniazide and pyrazinamide tablets are currently relatively efficacious antituberculosis drugs. In the present paper, near infrared spectroscopy (NIRS) with partial least squares (PLS) was applied to the simultaneous determination of rifampicin (RMP), isoniazide (INH) and pyrazinamide (PZA) contents in 5 varieties of anti-tuberculosis tablets. As the results showed, all of the models for the determination of RMP, INH and PZA contents applied the original NIR spectra. The most efficacious wavelength range for the determination of RMP contents was 1981-2195 nm, it was 1540-1717 nm and 2086-2197 nm for the determination of INH contents, and it was 1460-1537 nm, 1956-2022 nm and 2268-2393 nm for determination of PZA contents. The root mean square error of the calibration set obtained by cross-validation (RMSECV) of the optimum models for the quantitative analysis of RMP, INH and PZA contents was 0.0494, 0.0257 and 0.0307, respectively. Using these optimum models for the determination of RMP, INH and PZA contents in prediction set, the root mean square error of prediction set (RMSEP) was 0.0182, 0.0166 and 0.0134, respectively. The correlation coefficient (r(p)) between the predicted values and actual values was 0.9864, 0.9989 and 0.9993, respectively. These results demonstrated that this method was precise and reliable, and is significative for in situ measurement and the on-line quality control for anti-tuberculosis tablets production.
MeSH term(s)	Algorithms ; Antitubercular Agents/analysis ; Antitubercular Agents/chemistry ; Isoniazid/analysis ; Models, Chemical ; Principal Component Analysis ; Pyrazinamide/analysis ; Rifampin/analysis ; Rifampin/chemistry ; Spectroscopy, Near-Infrared/methods ; Tablets/analysis ; Tablets/chemistry
Chemical Substances	Antitubercular Agents ; Tablets ; Pyrazinamide (2KNI5N06TI) ; Isoniazid (V83O1VOZ8L) ; Rifampin (VJT6J7R4TR)
Language	Chinese
Publishing date	2008-08
Publishing country	China
Document type	English Abstract ; Journal Article
ISSN	1000-0593
ISSN	1000-0593
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: At-line monitoring of key parameters of nisin fermentation by near infrared spectroscopy, chemometric modeling and model improvement

Guo, Wei-Liang / Du, Yi-Ping / Zhou, Yong-Can / Yang, Shuang / Lu, Jia-Hui / Zhao, Hong-Yu / Wang, Yao / Teng, Li-Rong

World journal of microbiology and biotechnology. 2012 Mar., v. 28, no. 3

2012

Abstract: An analytical procedure has been developed for at-line (fast off-line) monitoring of 4 key parameters including nisin titer (NT), the concentration of reducing sugars, cell concentration and pH during a nisin fermentation process. This procedure is based ...

Abstract	An analytical procedure has been developed for at-line (fast off-line) monitoring of 4 key parameters including nisin titer (NT), the concentration of reducing sugars, cell concentration and pH during a nisin fermentation process. This procedure is based on near infrared (NIR) spectroscopy and Partial Least Squares (PLS). Samples without any preprocessing were collected at intervals of 1 h during fifteen batch of fermentations. These fermentation processes were implemented in 3 different 5 l fermentors at various conditions. NIR spectra of the samples were collected in 10 min. And then, PLS was used for modeling the relationship between NIR spectra and the key parameters which were determined by reference methods. Monte Carlo Partial Least Squares (MCPLS) was applied to identify the outliers and select the most efficacious methods for preprocessing spectra, wavelengths and the suitable number of latent variables (n LV). Then, the optimum models for determining NT, concentration of reducing sugars, cell concentration and pH were established. The correlation coefficients of calibration set (R c) were 0.8255, 0.9000, 0.9883 and 0.9581, respectively. These results demonstrated that this method can be successfully applied to at-line monitor of NT, concentration of reducing sugars, cell concentration and pH during nisin fermentation processes.
Keywords	batch fermentation ; correlation ; least squares ; models ; monitoring ; near-infrared spectroscopy ; nisin ; pH ; reducing sugars ; sampling
Language	English
Dates of publication	2012-03
Size	p. 993-1002.
Publishing place	Springer-Verlag
Document type	Article
ZDB-ID	1499109-3
ISSN	1573-0972 ; 0959-3993
ISSN (online)	1573-0972
ISSN	0959-3993
DOI	10.1007/s11274-011-0897-x
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article ; Online: At-line monitoring of key parameters of nisin fermentation by near infrared spectroscopy, chemometric modeling and model improvement.

Guo, Wei-Liang / Du, Yi-Ping / Zhou, Yong-Can / Yang, Shuang / Lu, Jia-Hui / Zhao, Hong-Yu / Wang, Yao / Teng, Li-Rong

World journal of microbiology & biotechnology

2011 Volume 28, Issue 3, Page(s) 993–1002

Abstract	An analytical procedure has been developed for at-line (fast off-line) monitoring of 4 key parameters including nisin titer (NT), the concentration of reducing sugars, cell concentration and pH during a nisin fermentation process. This procedure is based on near infrared (NIR) spectroscopy and Partial Least Squares (PLS). Samples without any preprocessing were collected at intervals of 1 h during fifteen batch of fermentations. These fermentation processes were implemented in 3 different 5 l fermentors at various conditions. NIR spectra of the samples were collected in 10 min. And then, PLS was used for modeling the relationship between NIR spectra and the key parameters which were determined by reference methods. Monte Carlo Partial Least Squares (MCPLS) was applied to identify the outliers and select the most efficacious methods for preprocessing spectra, wavelengths and the suitable number of latent variables (n (LV)). Then, the optimum models for determining NT, concentration of reducing sugars, cell concentration and pH were established. The correlation coefficients of calibration set (R (c)) were 0.8255, 0.9000, 0.9883 and 0.9581, respectively. These results demonstrated that this method can be successfully applied to at-line monitor of NT, concentration of reducing sugars, cell concentration and pH during nisin fermentation processes.
MeSH term(s)	Biotechnology/methods ; Culture Media/chemistry ; Fermentation ; Hydrogen-Ion Concentration ; Lactococcus lactis/growth & development ; Lactococcus lactis/metabolism ; Models, Statistical ; Nisin/metabolism ; Spectroscopy, Near-Infrared/methods
Chemical Substances	Culture Media ; Nisin (1414-45-5)
Language	English
Publishing date	2011-10-09
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1499109-3
ISSN	1573-0972 ; 0959-3993
ISSN (online)	1573-0972
ISSN	0959-3993
DOI	10.1007/s11274-011-0897-x
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Details ▾
- Full text online
- Order with fees

Article ; Online: Biodegradable poly(D, L-lactide-co-glycolide) (PLGA) microspheres for sustained release of risperidone: Zero-order release formulation.

Su, Zheng-Xing / Shi, Ya-Nan / Teng, Le-Sheng / Li, Xiang / Wang, Le-xi / Meng, Qing-Fan / Teng, Li-Rong / Li, You-Xin

Pharmaceutical development and technology

2011 Volume 16, Issue 4, Page(s) 377–384

Abstract: The preparation and investigation of sustained-release risperidone-encapsulated microspheres using erodible poly(D, L-lactide-co-glycolide) (PLGA) of lower molecular weight were performed and compared to that of commercial Risperdal Consta™ for the ... ...

Abstract	The preparation and investigation of sustained-release risperidone-encapsulated microspheres using erodible poly(D, L-lactide-co-glycolide) (PLGA) of lower molecular weight were performed and compared to that of commercial Risperdal Consta™ for the treatment of schizophrenia. The research included screening and optimizing of suitable commercial polymers of lower molecular weight PLGA50/50 or the blends of these PLGA polymers to prepare microspheres with zero-order release kinetics properties. Solvent evaporation method was applied here while studies of the risperidone loaded microsphere were carried out on its drug encapsulation capacity, morphology, particle size, as well as in vitro release profiles. Results showed that microspheres prepared using 50504A PLGA or blends of 5050-type PLGAs exerted spherical and smooth morphology, with a higher encapsulation efficiency and nearly zero-order release kinetics. These optimized microspheres showed great potential for a better depot preparation than the marketed Risperdal Consta™, which could further improve the patient compliance.
MeSH term(s)	Antipsychotic Agents/administration & dosage ; Antipsychotic Agents/pharmacokinetics ; Chemistry, Pharmaceutical/methods ; Delayed-Action Preparations/chemistry ; Delayed-Action Preparations/pharmacokinetics ; Lactic Acid/chemistry ; Microspheres ; Molecular Weight ; Particle Size ; Polyglycolic Acid/chemistry ; Risperidone/administration & dosage ; Risperidone/pharmacokinetics ; Schizophrenia/drug therapy ; Solubility
Chemical Substances	Antipsychotic Agents ; Delayed-Action Preparations ; polylactic acid-polyglycolic acid copolymer ; Polyglycolic Acid (26009-03-0) ; Lactic Acid (33X04XA5AT) ; Risperidone (L6UH7ZF8HC)
Language	English
Publishing date	2011-08
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1331774-x
ISSN	1097-9867 ; 1083-7450
ISSN (online)	1097-9867
ISSN	1083-7450
DOI	10.3109/10837451003739297
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 5043: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: [Effect of different delivery modes on the short-term function of the pelvic floor].

Long, Yan / Bian, Xu-ming / Zhu, Lan / Teng, Li-rong / Li, Lei / Lang, Jing-he

Zhonghua fu chan ke za zhi

2007 Volume 42, Issue 12, Page(s) 808–811

Abstract: Objective: To investigate the effect of different delivery modes and related obstetric factors on the short-term function of the pelvic floor.: Methods: One hundred and twenty healthy primiparae women were interviewed at 6-8 weeks postpartum, with 72 ...

Abstract	Objective: To investigate the effect of different delivery modes and related obstetric factors on the short-term function of the pelvic floor. Methods: One hundred and twenty healthy primiparae women were interviewed at 6-8 weeks postpartum, with 72 women in the vaginal delivery group and 48 women in the elective cesarean section group. Questionnaire on stress urinary incontinence and measurement of diastolic and contractive function of the pelvic floor muscles by electromyogram (EMG) were used for the evaluation and comparison. Results: The prevalence of stress urinary incontinence in the vaginal delivery group and the elective cesarean section group was 21% and 10% (P = 0. 134) ,respectively. The values of the right act, right work, and average work surveyed by EMG in vaginal delivery group were significantly lower than those in cesarean section group (right act, 12.9 +/- 0.8 vs. 17.3 +/- 1.7, P < 0.05; right work, 59 +/- 5 vs. 95 +/- 17, P = 0.02; average work, 78 +/- 5 vs. 109 +/- 15, P < 0.05). Maternal age, body mass index (BMI) before labor, incontinence during pregnancy, neonatal birth weight and longer duration of second stage were risk factors for stress urinary incontinence postpartum. Incision length of episiotomy, duration of the first stage, and BMI before and (or) after pregnancy affected function of pelvic floor muscles markedly. Conclusion: The prevalence of stress urinary incontinence after different delivery modes is similar. Some obstetric factors have effects on both stress urinary incontinence postpartum and the function of pelvic floor.
MeSH term(s)	Cesarean Section/methods ; Cesarean Section/psychology ; Female ; Humans ; Obstetric Labor Complications ; Pelvic Floor/physiology ; Postpartum Period/physiology ; Pregnancy ; Urinary Incontinence/physiopathology
Language	Chinese
Publishing date	2007-12
Publishing country	China
Document type	English Abstract ; Journal Article
ZDB-ID	604570-4
ISSN	0529-567X
ISSN	0529-567X
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1619: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 324: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: [Study on the detection of active ingredient contents of Paecilomyces hepiali mycelium via near infrared spectroscopy].

Teng, Wei-Zhuo / Song, Jia / Meng, Fan-Xin / Meng, Qing-Fan / Lu, Jia-Hui / Hu, Shuang / Teng, Li-Rong / Wang, Di / Xie, Jing

Guang pu xue yu guang pu fen xi = Guang pu

2014 Volume 34, Issue 10, Page(s) 2645–2651

Abstract: Partial least squares (PLS) and radial basis function neural network (RBFNN) combined with near infrared spectros- copy (NIR) were applied to develop models for cordycepic acid, polysaccharide and adenosine analysis in Paecilomyces hepialid fermentation ... ...

Abstract	Partial least squares (PLS) and radial basis function neural network (RBFNN) combined with near infrared spectros- copy (NIR) were applied to develop models for cordycepic acid, polysaccharide and adenosine analysis in Paecilomyces hepialid fermentation mycelium. The developed models possess well generalization and predictive ability which can be applied for crude drugs and related productions determination. During the experiment, 214 Paecilomyces hepialid mycelium samples were obtained via chemical mutagenesis combined with submerged fermentation. The contents of cordycepic acid, polysaccharide and adenosine were determined via traditional methods and the near infrared spectroscopy data were collected. The outliers were removed and the numbers of calibration set were confirmed via Monte Carlo partial least square (MCPLS) method. Based on the values of degree of approach (Da), both moving window partial least squares (MWPLS) and moving window radial basis function neural network (MWRBFNN) were applied to optimize characteristic wavelength variables, optimum preprocessing methods and other important variables in the models. After comparison, the RBFNN, RBFNN and PLS models were developed successfully for cordycepic acid, polysaccharide and adenosine detection, and the correlation between reference values and predictive values in both calibration set (R2c) and validation set (R2p) of optimum models was 0.9417 and 0.9663, 0.9803 and 0.9850, and 0.9761 and 0.9728, respectively. All the data suggest that these models possess well fitness and predictive ability.
MeSH term(s)	Adenosine/analysis ; Calibration ; Fermentation ; Least-Squares Analysis ; Models, Theoretical ; Mycelium/chemistry ; Neural Networks (Computer) ; Paecilomyces/chemistry ; Polysaccharides/analysis ; Spectroscopy, Near-Infrared
Chemical Substances	Polysaccharides ; Adenosine (K72T3FS567)
Language	Chinese
Publishing date	2014-10
Publishing country	China
Document type	English Abstract ; Journal Article
ISSN	1000-0593
ISSN	1000-0593
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED