LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 8 of total 8

Search options

  1. Article ; Online: Metalloprotein-Specific or Critical Amino Acid Residues

    Dandan Li / Tengbing He / Muhammad Saleem / Guandi He

    International Journal of Molecular Sciences, Vol 23, Iss 1734, p

    Perspectives on Plant-Precise Detoxification and Recognition Mechanisms under Cadmium Stress

    2022  Volume 1734

    Abstract: Cadmium (Cd) pollution in cultivated land is caused by irresistible geological factors and human activities; intense diffusion and migration have seriously affected the safety of food crops. Plants have evolved mechanisms to control excessive influx of ... ...

    Abstract Cadmium (Cd) pollution in cultivated land is caused by irresistible geological factors and human activities; intense diffusion and migration have seriously affected the safety of food crops. Plants have evolved mechanisms to control excessive influx of Cd in the environment, such as directional transport, chelation and detoxification. This is done by some specific metalloproteins, whose key amino acid motifs have been investigated by scientists one by one. The application of powerful cell biology, crystal structure science, and molecular probe targeted labeling technology has identified a series of protein families involved in the influx, transport and detoxification of the heavy metal Cd. This review summarizes them as influx proteins (NRAMP, ZIP), chelating proteins (MT, PDF), vacuolar proteins (CAX, ABCC, MTP), long-distance transport proteins (OPT, HMA) and efflux proteins (PCR, ABCG). We selected representative proteins from each family, and compared their amino acid sequence, motif structure, subcellular location, tissue specific distribution and other characteristics of differences and common points, so as to summarize the key residues of the Cd binding target. Then, we explain its special mechanism of action from the molecular structure. In conclusion, this review is expected to provide a reference for the exploration of key amino acid targets of Cd, and lay a foundation for the intelligent design and breeding of crops with high/low Cd accumulation.
    Keywords Cd transport and detoxification ; H/DXXXD ; cysteine-related residues ; functional protein ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 580
    Language English
    Publishing date 2022-02-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  2. Article ; Online: The Expression of the StNRAMP2 Gene Determined the Accumulation of Cadmium in Different Tissues of Potato

    Yule Zhang / Tengbing He / Weijun Tian / Yabei Xia / Yeqing He / Minmin Su / Guandi He

    International Journal of Molecular Sciences, Vol 24, Iss 9322, p

    2023  Volume 9322

    Abstract: Cadmium (Cd) is a toxic metal that threatens human health when enriched in crops. NRAMP s are a family of natural macrophage proteins reported to play a key role in Cd transport in plants. In order to explore the gene regulation mechanism of potato under ...

    Abstract Cadmium (Cd) is a toxic metal that threatens human health when enriched in crops. NRAMP s are a family of natural macrophage proteins reported to play a key role in Cd transport in plants. In order to explore the gene regulation mechanism of potato under Cd stress and the role of NRAMP s family in it, this study analyzed the gene expression differences of two different Cd accumulation levels in potato after 7 days of 50 mg/kg Cd stress and screened out the key genes that may play a major role in the differential accumulation of Cd in different varieties. Additionally, StNRAMP2 was selected for verification. Further verification showed that the StNRAMP2 gene plays an important role in the accumulation of Cd in potato. Interestingly, silencing StNRAMP2 increased Cd accumulation in tubers but significantly decreased Cd accumulation in other sites, suggesting a critical role of StNRAMP2 in Cd uptake and transport in potatoes. To further confirm this conclusion, we performed heterologous expression experiments in which overexpression of StNRAMP2 gene in tomato resulted in a threefold increase in Cd content, which further confirmed the important role of StNRAMP2 in the process of Cd accumulation compared with wild-type plants. In addition, we found that the addition of Cd to the soil increased the activity of the plant antioxidant enzyme system, and silencing StNRAMP2 partially reversed this effect. This suggests that the StNRAMP2 gene plays an important role in plant stress tolerance, and future studies could further explore the role of this gene in other environmental stresses. In conclusion, the results of this study improve the understanding of the mechanism of Cd accumulation in potato and provide experimental basis for remediation of Cd pollution.
    Keywords transcriptome ; functional verification ; heterologous expression ; VIGS ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 580
    Language English
    Publishing date 2023-05-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article ; Online: Response of GHG emissions to interactions of temperature and drying in the karst wetland of the Yunnan-Guizhou Plateau

    Yan He / Tao Zhang / Qiumei Zhao / Xiaoye Gao / Tengbing He / Shimei Yang

    Frontiers in Environmental Science, Vol

    2022  Volume 10

    Abstract: Hydrothermal fluctuation is the major driving factor affecting greenhouse gas (GHG) emissions in wetlands, but how wetland drying regulates the temperature dependence of GHG emissions remains uncertain. An experimental incubation was carried out to study ...

    Abstract Hydrothermal fluctuation is the major driving factor affecting greenhouse gas (GHG) emissions in wetlands, but how wetland drying regulates the temperature dependence of GHG emissions remains uncertain. An experimental incubation was carried out to study the interaction effects of temperature (5, 10, 15, 20°C) and moisture (40%, 60%, 100% WHC) on soil GHG emissions in a karst wetland. The results showed that: 1) the cumulative CO2 and N2O emissions and global warming potential (GWP) increased with increasing temperature but decreased with soil drying. 2) There was a decreasing contribution of CO2 and an increasing contribution of N2O to GWP with increasing temperature and moisture. 3) Soil CO2 and N2O emissions and GWP were positively related to urease activity and negatively related to pH, soil organic matter and catalase. Soil CH4 emissions were positively related to soil microbial biomass C and N. The hydrothermal changes, soil properties and their interaction explained 26.86%, 9.46% and 49.61% of the variation in GWP. Our results indicate that hydrothermal fluctuation has a significant effect on total GHG emissions by regulating soil properties.
    Keywords hydrothermal fluctuation ; CH4 ; N2O ; global warming potential ; soil incubation ; Environmental sciences ; GE1-350
    Language English
    Publishing date 2022-11-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  4. Article ; Online: Different composites inhibit Cd accumulation in grains under the rice-oilseed rape rotation mode of karst area

    Fei Lou / Tianling Fu / Guandi He / Weijun Tian / Jichang Wen / Mingfang Yang / Xiaoliao Wei / Yeqing He / Tengbing He

    Ecotoxicology and Environmental Safety, Vol 256, Iss , Pp 114884- (2023)

    A field study

    2023  

    Abstract: Ensuring the safe production of food and oil crops in soils with elevated cadmium (Cd) content in karst regions is crucial. We tested a field experiment to examine the long-term remediation effects of compound microorganisms (CM), strong anion exchange ... ...

    Abstract Ensuring the safe production of food and oil crops in soils with elevated cadmium (Cd) content in karst regions is crucial. We tested a field experiment to examine the long-term remediation effects of compound microorganisms (CM), strong anion exchange adsorbent (SAX), processed oyster shell (POS), and composite humic acids (CHA) on Cd contamination in paddy fields under a rice-oilseed rape rotation system. In comparison to the control group (CK), the application of amendments significantly increased soil pH, cation exchange capacity (CEC), and soil organic matter (SOM) content while markedly decreasing the content of available Cd (ACd). During the rice cultivation season, Cd was predominantly concentrated in the roots. Relative to the control (CK), the Cd content in each organ was significantly reduced. The Cd content in brown rice decreased by 19.18–85.45%. The Cd content in brown rice following different treatments exhibited the order of CM > POS > CHA > SAX, which was lower than the Chinese Food Safety Standard (GB 2762–2017) (0.20 mg/kg). Intriguingly, during the oilseed rape cultivation season, we discovered that oilseed rape possesses potential phytoremediation capabilities, with Cd mainly accumulating in roots and stems. Notably, CHA treatment alone significantly decreased the Cd content in oilseed rape grains to 0.156 mg/kg. CHA treatment also maintained soil pH and SOM content, consistently reduced soil ACd content, and stabilized Cd content in RSF within the rice-oilseed rape rotation system. Importantly, CHA treatment not only enhances crop production but also has a low total cost (1255.230 US$/hm2). Our research demonstrated that CHA provides a consistent and stable remediation effect on Cd-contaminated rice fields within the crop rotation system, as evidenced by the analysis of Cd reduction efficiency, crop yield, soil environmental change, and total cost. These findings offer valuable guidance for sustainable soil utilization and safe production of grain and oil crops in the context of ...
    Keywords Cadmium ; Paddy soil ; Composite amendments ; Paddy-upland rotation ; Total cost ; Environmental pollution ; TD172-193.5 ; Environmental sciences ; GE1-350
    Subject code 630
    Language English
    Publishing date 2023-05-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  5. Article ; Online: Feeding controls H

    Ruxiang, Cen / Ruiying, Chen / Tianyun, Pu / Chunyan, Huang / Tengbing, He / Guangliang, Tian

    Bioresources and bioprocessing

    2022  Volume 9, Issue 1, Page(s) 79

    Abstract: In this study, a high frequency monitoring method was used to assess how semi-continuous feeding affects ... ...

    Abstract In this study, a high frequency monitoring method was used to assess how semi-continuous feeding affects H
    Language English
    Publishing date 2022-08-04
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2785482-6
    ISSN 2197-4365 ; 2197-4365
    ISSN (online) 2197-4365
    ISSN 2197-4365
    DOI 10.1186/s40643-022-00567-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article ; Online: Comparative and Systematic Omics Revealed Low Cd Accumulation of Potato StMTP 9 in Yeast

    Dandan Li / Guandi He / Weijun Tian / Muhammad Saleem / Yun Huang / Lulu Meng / Danxia Wu / Tengbing He

    International Journal of Molecular Sciences, Vol 22, Iss 10478, p

    Suggesting a New Mechanism for Heavy Metal Detoxification

    2021  Volume 10478

    Abstract: The metal tolerance protein (MTP) family is a very old family with evolutionary conservation and less specific amplification. It seems to retain the original functions of the ancestral genes and plays an important role in maintaining metal homeostasis in ...

    Abstract The metal tolerance protein (MTP) family is a very old family with evolutionary conservation and less specific amplification. It seems to retain the original functions of the ancestral genes and plays an important role in maintaining metal homeostasis in plant cells. We identified the potato MTP family members for the first time, the specific and conservative StMPT s were discovered by using systematic and comparative omics. To be surprised, members of the StMTP family seem to have mutated before the evolution of dicotyledon and monocotyledon, and even the loss of the entire subfamily (subfamily G6, G7). Interestingly, StMTP9 represents the conserved structure of the entire subfamily involved in toxic metal regulation. However, the gene structure and transmembrane domain of StMTP8 have undergone specific evolution, showing that the transmembrane domain (Motif13) located at the NH 2 terminal has been replaced by the signal peptide domain, so it was selected as the control gene of StMTP9 . Through real-time fluorescence quantitative analysis of StMTP s under Cd and Zn stress, a co-expression network was constructed, and it was found that StMTP9 responded significantly to Cd stress, while StMTP8 did the opposite. What excites us is that by introducing StMTPs 8/9 into the ∆ycf1 yeast cadmium-sensitive mutant strain, the functional complementation experiment proved that StMTPs 8/9 can restore Cd tolerance. In particular, StMTP9 can greatly reduce the cadmium content in yeast cells, while StMTP8 cannot. These findings provide a reference for further research on the molecular mechanism of potato toxic metal accumulation.
    Keywords metal tolerance proteins ; specific evolutionary analysis ; cadmium tolerance ; StMTP9 ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Language English
    Publishing date 2021-09-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  7. Article: Extent to which pH and topographic factors control soil organic carbon level in dry farming cropland soils of the mountainous region of Southwest China

    Tu, Chenglong / Pete Smith / Tengbing He / Xiaohui Lu / Ya Luo

    Catena. 2018 Apr., v. 163

    2018  

    Abstract: Soil organic carbon (SOC) in agricultural land is influenced greatly by indeterminate human activity, making it difficult to understand the spatial pattern of SOC. Soil pH and topographic conditions are key indices in the Chinese Soil Genetic ... ...

    Abstract Soil organic carbon (SOC) in agricultural land is influenced greatly by indeterminate human activity, making it difficult to understand the spatial pattern of SOC. Soil pH and topographic conditions are key indices in the Chinese Soil Genetic Classification System (CSGCS) and manage some critical factors that control the dynamics of SOC either directly or indirectly. To identify the extent to which pH and topographic factors control SOC levels in dry farming cropland soils of the mountainous region of Southwest China, we compared the differences along topographic gradients, and analysed the contribution of different factors in determining SOC status using analysis of variance (ANOVA) and linear regression. Our results indicated the SOC levels ranged from 10.46g•kg−1 to 37.60g•kg−1 and were significantly correlated with soil pH, landscape position, slope and elevation (p<0.05). On a large scale, the combined effects of landscape position and elevation contributed to fluctuating SOC levels along the elevation gradient. SOC levels slightly, but significantly, decreased from base to summit. The difference of SOC levels along a 200m elevation gradient exhibited statistical significance (p<0.05). A slope range, from 0 to 42°, was categorized into three groups, namely, 5° to 15°, 15° to 30° and others. The slope range 15° to 30° had significantly greater SOC values than the other groups. These variables could all together explain approximately 40% of total variation in SOC, of which approximately 70% was attributable to soil pH, suggesting soil pH plays a key role in forming the spatial pattern of SOC levels in dry farming cropland soils of the mountainous region of Southwest China. The combined effect of landscape position and elevation could further explain 7.3% of SOC variation, which is more apparent than the effect of elevation alone.
    Keywords altitude ; cropland ; dryland farming ; humans ; landscape position ; mountains ; regression analysis ; soil organic carbon ; soil pH ; topographic slope ; China
    Language English
    Dates of publication 2018-04
    Size p. 204-209.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 519608-5
    ISSN 1872-6887 ; 0008-7769 ; 0341-8162
    ISSN (online) 1872-6887 ; 0008-7769
    ISSN 0341-8162
    DOI 10.1016/j.catena.2017.12.028
    Database NAL-Catalogue (AGRICOLA)

    Full text online

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 3389: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  8. Article: Soil nitrogen leaching decreases as biogas slurry DOC/N ratio increases

    Cheng, Jianbo / Lei Huang / Man Yi / Renjun Liao / Renlu Liu / Tengbing He / Tianling Fu / Xiangying Li / Yucheng Chen / Zhimin Yang

    Applied soil ecology. 2017 Mar., v. 111

    2017  

    Abstract: Previous work has measured high rates of nitrogen (N) leaching during biogas slurry irrigation. Here we tested whether increasing the ratio of dissolved organic carbon (DOC) to N in biogas slurry would decrease N leaching by applying biogas slurries with ...

    Abstract Previous work has measured high rates of nitrogen (N) leaching during biogas slurry irrigation. Here we tested whether increasing the ratio of dissolved organic carbon (DOC) to N in biogas slurry would decrease N leaching by applying biogas slurries with DOC/N ratios of 0.9, 2.3, 7.3, 12.3 and 17.3 to soil columns in the laboratory. Increasing the DOC/N ratio to at least 7.3 reduced N leaching by approximately 91%, likely because denitrification and nitrate (NO3−) consumption were increased in the DOC/N 7.3 treatment and ammonium immobilization increased (and NO3− production decreased) in the DOC/N 12.3 and 17.3 treatments. Additionally, all the detected soil nitrifiers—uncultured Nitrosomonadaceae, Nitrospira, Nitrosospira and Nitrosomonas—were promoted in the DOC/N 0.9–7.3 treatments and inhibited in the DOC/N 12.3 and 17.3 treatments. According to the results of weighted gene co-expression network analysis (WGCNA), it is likely that the soil nitrification was promoted by Acidobacteriaceae (Subgroup_1_uncultured), Rhizomicrobium, Massilia, uncultured Nitrosomonadaceae and Nitrospira but inhibited by Trichococcus.
    Keywords Acidobacteriaceae ; biogas ; denitrification ; dissolved organic carbon ; genes ; irrigation ; leaching ; Massilia ; nitrates ; nitrification ; nitrogen ; Nitrosospira ; slurries ; soil ; Trichococcus
    Language English
    Dates of publication 2017-03
    Size p. 105-113.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1196758-4
    ISSN 0929-1393
    ISSN 0929-1393
    DOI 10.1016/j.apsoil.2016.12.001
    Database NAL-Catalogue (AGRICOLA)

    Full text online

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 5926: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top