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  1. Article: Characterization of monoclonal antibodies by a fast and easy liquid chromatography–mass spectrometry time-of-flight analysis on culture supernatant

    Henninot, Antoine / Alexandre Fontayne / Aurelie Terrier / Benoit Deprez / Julie Charton / Rémi Urbain / Terence Beghyn

    Analytical biochemistry. 2015 Dec. 15, v. 491

    2015  

    Abstract: Rapid and efficient structural analysis is key to the development of new monoclonal antibodies. We have developed a fast and easy process to obtain mass spectrometry profiles of antibodies from culture supernatant. Treatment of the supernatant with IdeS ... ...

    Abstract Rapid and efficient structural analysis is key to the development of new monoclonal antibodies. We have developed a fast and easy process to obtain mass spectrometry profiles of antibodies from culture supernatant. Treatment of the supernatant with IdeS generates three fragments of 25 kDa that can be analyzed by liquid chromatography–mass spectrometry time-of-flight (LC–MS TOF) in one run: LC, Fd, and Fc/2. This process gives rapid access to isoform and glycoform profiles. To specifically measure the fucosylation yield, we included a one-pot treatment with EndoS that removes the distal glycan heterogeneity. Our process was successfully compared with high-performance capillary electrophoresis with laser-induced fluorescence detection (HPCE–LIF), currently considered as the “gold standard” method.
    Keywords capillary electrophoresis ; fluorescence ; liquid chromatography ; mass spectrometry ; monoclonal antibodies
    Language English
    Dates of publication 2015-1215
    Size p. 52-54.
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 1110-1
    ISSN 1096-0309 ; 0003-2697
    ISSN (online) 1096-0309
    ISSN 0003-2697
    DOI 10.1016/j.ab.2015.08.006
    Database NAL-Catalogue (AGRICOLA)

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  2. Article ; Online: Clofoctol inhibits SARS-CoV-2 replication and reduces lung pathology in mice.

    Sandrine Belouzard / Arnaud Machelart / Valentin Sencio / Thibaut Vausselin / Eik Hoffmann / Nathalie Deboosere / Yves Rouillé / Lowiese Desmarets / Karin Séron / Adeline Danneels / Cyril Robil / Loic Belloy / Camille Moreau / Catherine Piveteau / Alexandre Biela / Alexandre Vandeputte / Séverine Heumel / Lucie Deruyter / Julie Dumont /
    Florence Leroux / Ilka Engelmann / Enagnon Kazali Alidjinou / Didier Hober / Priscille Brodin / Terence Beghyn / François Trottein / Benoit Deprez / Jean Dubuisson

    PLoS Pathogens, Vol 18, Iss 5, p e

    2022  Volume 1010498

    Abstract: Drug repurposing has the advantage of shortening regulatory preclinical development steps. Here, we screened a library of drug compounds, already registered in one or several geographical areas, to identify those exhibiting antiviral activity against ... ...

    Abstract Drug repurposing has the advantage of shortening regulatory preclinical development steps. Here, we screened a library of drug compounds, already registered in one or several geographical areas, to identify those exhibiting antiviral activity against SARS-CoV-2 with relevant potency. Of the 1,942 compounds tested, 21 exhibited a substantial antiviral activity in Vero-81 cells. Among them, clofoctol, an antibacterial drug used for the treatment of bacterial respiratory tract infections, was further investigated due to its favorable safety profile and pharmacokinetic properties. Notably, the peak concentration of clofoctol that can be achieved in human lungs is more than 20 times higher than its IC50 measured against SARS-CoV-2 in human pulmonary cells. This compound inhibits SARS-CoV-2 at a post-entry step. Lastly, therapeutic treatment of human ACE2 receptor transgenic mice decreased viral load, reduced inflammatory gene expression and lowered pulmonary pathology. Altogether, these data strongly support clofoctol as a therapeutic candidate for the treatment of COVID-19 patients.
    Keywords Immunologic diseases. Allergy ; RC581-607 ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2022-05-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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