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  1. Article ; Online: Culturing clinical Methanobrevibacter smithii using GG medium in a minimal anaerobe atmosphere

    Pilliol, Virginie / Guindo, Cheick Oumar / Terrer, Elodie / Aboudharam, Gérard / Drancourt, Michel / Grine, Ghiles

    Journal of Microbiological Methods. 2023 Apr., v. 207 p.106704-

    2023  

    Abstract: Methanobrevibacter smithii (M. smithii), the most prevalent and abundant gut methanogen, detoxifies hydrogen into methane and is, therefore, of paramount importance for the equilibrium of the gut microbiota. The isolation by culture of M. smithii has ... ...

    Abstract Methanobrevibacter smithii (M. smithii), the most prevalent and abundant gut methanogen, detoxifies hydrogen into methane and is, therefore, of paramount importance for the equilibrium of the gut microbiota. The isolation by culture of M. smithii has routinely relied upon hydrogen‑carbon dioxide-enriched, oxygen-deprived atmospheres. In this study, we developed a medium referred to as “GG”, which allowed for M. smithii growth and isolation by culture in an oxygen-deprived atmosphere, with no supply of either hydrogen or carbon dioxide, making it easier to detect M. smithii by culture in clinical microbiology laboratories.
    Keywords Methanobrevibacter smithii ; carbon dioxide ; digestive system ; hydrogen ; intestinal microorganisms ; methane ; methanogens ; GG medium ; Atmosphere ; Clinical microbiology
    Language English
    Dates of publication 2023-04
    Publishing place Elsevier B.V.
    Document type Article ; Online
    Note Use and reproduction
    ZDB-ID 604916-3
    ISSN 1872-8359 ; 0167-7012
    ISSN (online) 1872-8359
    ISSN 0167-7012
    DOI 10.1016/j.mimet.2023.106704
    Database NAL-Catalogue (AGRICOLA)

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  2. Article: Methanobrevibacter massiliense

    Pilliol, Virginie / Beye, Mamadou / Terlier, Laureline / Balmelle, Julien / Kacel, Idir / Lan, Romain / Aboudharam, Gérard / Grine, Ghiles / Terrer, Elodie

    Microorganisms

    2024  Volume 12, Issue 1

    Abstract: Among oral microbiota methanogens, ...

    Abstract Among oral microbiota methanogens,
    Language English
    Publishing date 2024-01-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms12010215
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Retraction Note: Peri-implantitis-associated methanogens: a preliminary report.

    Belkacemi, Souad / Mazel, Anthony / Tardivo, Delphine / Tavitian, Patrick / Stephan, Grégory / Bianca, Giancarlo / Terrer, Elodie / Drancourt, Michel / Aboudharam, Gérard

    Scientific reports

    2024  Volume 14, Issue 1, Page(s) 1355

    Language English
    Publishing date 2024-01-16
    Publishing country England
    Document type Retraction of Publication
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-024-51888-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Culturing the Human Oral Microbiota, Updating Methodologies and Cultivation Techniques.

    Khelaifia, Saber / Virginie, Pilliol / Belkacemi, Souad / Tassery, Herve / Terrer, Elodie / Aboudharam, Gérard

    Microorganisms

    2023  Volume 11, Issue 4

    Abstract: Recent years have been marked by a paradigm shift in the study of the human microbiota, with a re-emergence of culture-dependent approaches. Numerous studies have been devoted to the human microbiota, while studies on the oral microbiota still remain ... ...

    Abstract Recent years have been marked by a paradigm shift in the study of the human microbiota, with a re-emergence of culture-dependent approaches. Numerous studies have been devoted to the human microbiota, while studies on the oral microbiota still remain limited. Indeed, various techniques described in the literature may enable an exhaustive study of the microbial composition of a complex ecosystem. In this article, we report different methodologies and culture media described in the literature that can be applied to study the oral microbiota by culture. We report on specific methodologies for targeted culture and specific culture techniques and selection methodologies for cultivating members of the three kingdoms of life commonly found in the human oral cavity, namely, eukaryota, bacteria and archaea. This bibliographic review aims to bring together the various techniques described in the literature, enabling a comprehensive study of the oral microbiota in order to demonstrate its involvement in oral health and diseases.
    Language English
    Publishing date 2023-03-24
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms11040836
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Culturing clinical Methanobrevibacter smithii using GG medium in a minimal anaerobe atmosphere.

    Pilliol, Virginie / Guindo, Cheick Oumar / Terrer, Elodie / Aboudharam, Gérard / Drancourt, Michel / Grine, Ghiles

    Journal of microbiological methods

    2023  Volume 207, Page(s) 106704

    Abstract: Methanobrevibacter smithii (M. smithii), the most prevalent and abundant gut methanogen, detoxifies hydrogen into methane and is, therefore, of paramount importance for the equilibrium of the gut microbiota. The isolation by culture of M. smithii has ... ...

    Abstract Methanobrevibacter smithii (M. smithii), the most prevalent and abundant gut methanogen, detoxifies hydrogen into methane and is, therefore, of paramount importance for the equilibrium of the gut microbiota. The isolation by culture of M. smithii has routinely relied upon hydrogen‑carbon dioxide-enriched, oxygen-deprived atmospheres. In this study, we developed a medium referred to as "GG", which allowed for M. smithii growth and isolation by culture in an oxygen-deprived atmosphere, with no supply of either hydrogen or carbon dioxide, making it easier to detect M. smithii by culture in clinical microbiology laboratories.
    MeSH term(s) Methanobrevibacter ; Carbon Dioxide ; Bacteria, Anaerobic ; Gastrointestinal Microbiome ; Hydrogen
    Chemical Substances Carbon Dioxide (142M471B3J) ; Hydrogen (7YNJ3PO35Z)
    Language English
    Publishing date 2023-03-11
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 604916-3
    ISSN 1872-8359 ; 0167-7012
    ISSN (online) 1872-8359
    ISSN 0167-7012
    DOI 10.1016/j.mimet.2023.106704
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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    In stock of ZB MED Cologne/Königswinter

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  6. Article ; Online: Candidatus

    Pilliol, Virginie / Morsli, Madjid / Terlier, Laureline / Hassani, Yasmine / Malat, Ihab / Guindo, Cheick Oumar / Davoust, Bernard / Lamglait, Benjamin / Drancourt, Michel / Aboudharam, Gérard / Grine, Ghiles / Terrer, Elodie

    Microbiology spectrum

    2024  Volume 12, Issue 2, Page(s) e0514122

    Abstract: Methanosphaera ... ...

    Abstract Methanosphaera stadtmanae
    MeSH term(s) Humans ; Animals ; Swine ; Macropodidae/genetics ; Methanobacteriaceae/genetics ; Methane ; Feces ; Hydrogen ; Ethanol ; Phylogeny ; RNA, Ribosomal, 16S/genetics
    Chemical Substances Methane (OP0UW79H66) ; Hydrogen (7YNJ3PO35Z) ; Ethanol (3K9958V90M) ; RNA, Ribosomal, 16S
    Language English
    Publishing date 2024-01-08
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.05141-22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Preventive management of carious lesions: from non-invasive to micro-invasive operative interventions.

    Tassery, Hervé / Miletic, Ivana / Turkun, Lezize Sebnem / Sauro, Salvatore / Gurgan, Sevil / Banerjee, Avijit / Basso, Matteo / Khelafia, Saber / Terrer, Elodie / Pilliol, Virginie / Slimani, Amel

    British dental journal

    2024  Volume 236, Issue 8, Page(s) 603–610

    Abstract: Understanding that dental carious lesions occur as a result of the action of micro-organisms in the dental plaque biofilm, where demineralisation on the tooth surface is the first sign of the disease, such incipient lesions can be treated using ... ...

    Abstract Understanding that dental carious lesions occur as a result of the action of micro-organisms in the dental plaque biofilm, where demineralisation on the tooth surface is the first sign of the disease, such incipient lesions can be treated using preventive, non-operative and minimally invasive operative dentistry. If the caries process is left unmanaged, the lesions progress towards cavitation, leading to more invasive treatments. This article discusses the principles of preventive, non-invasive and micro-invasive treatments of early carious lesions, outlining the clinical situations where these therapies can be applied.
    MeSH term(s) Humans ; Dental Caries/prevention & control ; Dental Caries/therapy ; Biofilms ; Cariostatic Agents/therapeutic use ; Dental Plaque/prevention & control ; Dental Restoration, Permanent/methods
    Chemical Substances Cariostatic Agents
    Language English
    Publishing date 2024-04-26
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 218090-x
    ISSN 1476-5373 ; 0007-0610
    ISSN (online) 1476-5373
    ISSN 0007-0610
    DOI 10.1038/s41415-024-7292-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.MO 447: Show issues

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  8. Article ; Online: Carious lesion detection technologies: factual clinical approaches.

    Slimani, Amel / Terrer, Elodie / Manton, David J / Tassery, Hervé

    British dental journal

    2020  Volume 229, Issue 7, Page(s) 432–442

    Abstract: Minimal intervention dentistry is now accepted as the contemporary approach for caries management. The development of adjunctive technologies to assist early lesion detection has led to widespread marketing of various devices over the past two decades. A ...

    Abstract Minimal intervention dentistry is now accepted as the contemporary approach for caries management. The development of adjunctive technologies to assist early lesion detection has led to widespread marketing of various devices over the past two decades. A thorough understanding of the clinical relevance and limitations of such devices is required to make valid interpretation of their results. This paper will discuss the most common commercially available carious lesion detection technologies in order to give dental practitioners clear information about the devices' scientific principles, advantages and limitations within a clinical context.
    MeSH term(s) Dental Care ; Dental Caries/diagnosis ; Dental Caries/therapy ; Dentists ; Humans ; Professional Role
    Language English
    Publishing date 2020-10-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 218090-x
    ISSN 1476-5373 ; 0007-0610
    ISSN (online) 1476-5373
    ISSN 0007-0610
    DOI 10.1038/s41415-020-2116-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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    In stock of ZB MED Cologne/Königswinter

    Ul III Zs.143: Show issues Location:
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  9. Article: Culture of salivary methanogens assisted by chemically produced hydrogen

    Guindo, Cheick O / Aboudharam, Gérard / Chabrière, Eric / Drancourt, Michel / Grine, Ghiles / Terrer, Elodie

    Anaerobe. 2020 Feb., v. 61

    2020  

    Abstract: Methanogen cultures require hydrogen produced by fermentative bacteria such as Bacteroides thetaiotaomicron (biological method). We developed an alternative method for hydrogen production using iron filings and acetic acid with the aim of cultivating ... ...

    Abstract Methanogen cultures require hydrogen produced by fermentative bacteria such as Bacteroides thetaiotaomicron (biological method). We developed an alternative method for hydrogen production using iron filings and acetic acid with the aim of cultivating methanogens more efficiently and more quickly (chemical method). We developed this new method with a reference strain of Methanobrevibacter oralis, compared the method to the biological reference method with a reference strain of Methanobrevibacter smithii and finally applied the method to 50 saliva samples. Methanogen colonies counted using ImageJ software were identified using epifluorescence optical microscopy, real-time PCR and PCR sequencing. For cultures containing pure strains of M. oralis and M. smithii, colonies appeared three days postinoculation with the chemical method versus nine days with the biological method. The average number of M. smithii colonies was significantly higher with the chemical method than with the biological method. There was no difference in the delay of observation of the first colonies in the saliva samples between the two methods. However, the average number of colonies was significantly higher with the biological method than with the chemical method at six days and nine days postinoculation (Student’s test, p = 0.005 and p = 0.04, respectively). The chemical method made it possible to isolate four strains of M. oralis and three strains of M. smithii from the 50 saliva samples. Establishing the chemical method will ease the routine isolation and culture of methanogens.
    Keywords acetic acid ; bacteria ; Bacteroides thetaiotaomicron ; computer software ; hydrogen ; hydrogen production ; iron ; light microscopy ; Methanobrevibacter smithii ; methanogens ; quantitative polymerase chain reaction ; saliva ; sequence analysis
    Language English
    Dates of publication 2020-02
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1237621-8
    ISSN 1075-9964
    ISSN 1075-9964
    DOI 10.1016/j.anaerobe.2019.102128
    Database NAL-Catalogue (AGRICOLA)

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  10. Article: Tobacco Smoking Affects the Salivary Gram-Positive Bacterial Population.

    Grine, Ghiles / Royer, Arthur / Terrer, Elodie / Diallo, Ousmane Oumou / Drancourt, Michel / Aboudharam, Gérard

    Frontiers in public health

    2019  Volume 7, Page(s) 196

    Abstract: The microbial communities of the oral fluid are in direct contact with tobacco smoke, which may thus affect these communities. Few culture-based studies have analyzed the effects of tobacco smoking on the oral fluid microbiota. Using bacterial culture we ...

    Abstract The microbial communities of the oral fluid are in direct contact with tobacco smoke, which may thus affect these communities. Few culture-based studies have analyzed the effects of tobacco smoking on the oral fluid microbiota. Using bacterial culture we investigated whether tobacco smoking altered the microbial diversity of the oral fluid, focusing on aerobic and facultative anaerobic Gram-positive bacteria otherwise comprising of major pathogens. Among 90 oral fluid specimens collected in 19 tobacco-smokers and 71 controls, the diversity did not significantly differ with age and with sex. However, diversity was significantly lower in tobacco-smokers (nine different species) than in non-smokers (18 different species) with all the species cultured in tabocco-smokers being also cultured in non-smokers. We isolated the human pathogen
    Language English
    Publishing date 2019-07-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2711781-9
    ISSN 2296-2565
    ISSN 2296-2565
    DOI 10.3389/fpubh.2019.00196
    Database MEDical Literature Analysis and Retrieval System OnLINE

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