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  1. Article ; Online: Resistance of Black Aspergilli Species from Grape Vineyards to SDHI, QoI, DMI, and Phenylpyrrole Fungicides.

    Testempasis, Stefanos I / Karaoglanidis, George S

    Journal of fungi (Basel, Switzerland)

    2023  Volume 9, Issue 2

    Abstract: Fungicide applications constitute a management practice that reduces the size of fungal populations and by acting as a genetic drift factor, may affect pathogen evolution. In a previous study, we showed that the farming system influenced the population ... ...

    Abstract Fungicide applications constitute a management practice that reduces the size of fungal populations and by acting as a genetic drift factor, may affect pathogen evolution. In a previous study, we showed that the farming system influenced the population structure of the
    Language English
    Publishing date 2023-02-07
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2784229-0
    ISSN 2309-608X ; 2309-608X
    ISSN (online) 2309-608X
    ISSN 2309-608X
    DOI 10.3390/jof9020221
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Conventional vs. organic vineyards: Black Aspergilli population structure, mycotoxigenic capacity and mycotoxin contamination assessment in wines, using a new Q-TOF MS-MS detection method

    Testempasis, Stefanos I. / Kamou, Nathalie N. / Papadakis, Emmanouil-Nikolaos / Menkissoglu-Spiroudi, Urania / Karaoglanidis, George S.

    Food control. 2022 June, v. 136

    2022  

    Abstract: Aspergillus bunch rot, caused by several mycotoxigenic species of Black Aspergilli section Nigri is one of the most severe diseases of grapevine, while mycotoxin contaminated products may pose a risk to the consumers health. During a 2-year (2018–2019) ... ...

    Abstract Aspergillus bunch rot, caused by several mycotoxigenic species of Black Aspergilli section Nigri is one of the most severe diseases of grapevine, while mycotoxin contaminated products may pose a risk to the consumers health. During a 2-year (2018–2019) survey, the impact of conventional and organic farming systems on Black Aspergilli population structure in Greek vineyards was thoroughly investigated. In total, 300 isolates of Aspergillus spp. were selected and identified by amplicon sequencing of the ITS-region, β-tubulin and calmodulin genes. Four different Aspergillus section Nigri species (A. tubingensis, A. uvarum, A. carbonarius and A. niger) were identified as the casual agents of the disease. During both sampling years, higher frequencies of A. tubingensis and A. uvarum were found in the organic and conventional vineyards, respectively. In vitro production of ochratoxin A (OTA) and fumonisin B₂, B₃ and B₄ (FB₂, FB₃, and FB₄) was evaluated in two selective media. The analysis revealed a low frequency of mycotoxigenic isolates, while, most of these isolates originated from conventional vineyards. As expected, A. carbonarious was identified as the main OTA producer (0.01–9.2 μg/g), whereas A. niger was the leading producer of FBs. In total, 74 wine samples originating from either organic or conventional vineyards of the same region, were analyzed using a new Quadrupole Time-of-Flight Mass Spectrometry (QTOF MS-MS) analytical method developed to detect and quantify Alternariol (AOH), Alternariol monomethyl ether (AME), and Citrinin (CIT) in addition to the aforementioned Aspergillus mycotoxins (OTA, FB₁, FB₂ FB₃), using a modified QuEChERS extraction protocol. Interestingly, a low frequency of mycotoxin-contaminated wines was detected. At the same time, fumonisins (FBs) were identified as the main mycotoxins in Greek wines. This is the first study deciphering the impact of conventional and organic farming systems on Aspergillus section Nigri species in Greek vineyards, suggesting that farming system may affect the species composition within the vineyard. Furthermore, evidence was provided for a higher risk of selection of Aspergillus strains with higher mycotoxigenic capacity in conventional vineyards, associated with higher risk of mycotoxin contamination in wines originating from these vineyards.
    Keywords Vitis ; calmodulin ; citrinin ; food safety ; mass spectrometry ; ochratoxin A ; population structure ; risk ; species diversity ; surveys ; vineyards ; wines
    Language English
    Dates of publication 2022-06
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1027805-9
    ISSN 0956-7135
    ISSN 0956-7135
    DOI 10.1016/j.foodcont.2022.108860
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 5255: Show issues

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  3. Article ; Online: Grapevine Trunk Diseases in Greece: Disease Incidence and Fungi Involved in Discrete Geographical Zones and Varieties.

    Testempasis, Stefanos I / Markakis, Emmanouil A / Tavlaki, Georgia I / Soultatos, Stefanos K / Tsoukas, Christos / Gkizi, Danai / Tzima, Aliki K / Paplomatas, Epameinondas / Karaoglanidis, Georgios S

    Journal of fungi (Basel, Switzerland)

    2023  Volume 10, Issue 1

    Abstract: A three-year survey was conducted to estimate the incidence of grapevine trunk diseases (GTDs) in Greece and identify fungi associated with the disease complex. In total, 310 vineyards in different geographical regions in northern, central, and southern ... ...

    Abstract A three-year survey was conducted to estimate the incidence of grapevine trunk diseases (GTDs) in Greece and identify fungi associated with the disease complex. In total, 310 vineyards in different geographical regions in northern, central, and southern Greece were surveyed, and 533 fungal strains were isolated from diseased vines. Morphological, physiological and molecular (5.8S rRNA gene-ITS sequencing) analyses revealed that isolates belonged to 35 distinct fungal genera, including well-known (e.g.,
    Language English
    Publishing date 2023-12-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2784229-0
    ISSN 2309-608X ; 2309-608X
    ISSN (online) 2309-608X
    ISSN 2309-608X
    DOI 10.3390/jof10010002
    Database MEDical Literature Analysis and Retrieval System OnLINE

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