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  1. Article ; Online: Development of a Sequence Searchable Database of Celiac Disease-Associated Peptides and Proteins for Risk Assessment of Novel Food Proteins.

    Amnuaycheewa, Plaimein / Abdelmoteleb, Mohamed / Wise, John / Bohle, Barbara / Ferreira, Fatima / Tetteh, Afua O / Taylor, Steve L / Goodman, Richard E

    Frontiers in allergy

    2022  Volume 3, Page(s) 900573

    Abstract: Celiac disease (CeD) is an autoimmune enteropathy induced by prolamin and glutelin proteins in wheat, barley, rye, and triticale recognized by genetically restricted major histocompatibility (MHC) receptors. Patients with CeD must avoid consuming these ... ...

    Abstract Celiac disease (CeD) is an autoimmune enteropathy induced by prolamin and glutelin proteins in wheat, barley, rye, and triticale recognized by genetically restricted major histocompatibility (MHC) receptors. Patients with CeD must avoid consuming these proteins. Regulators in Europe and the United States expect an evaluation of CeD risks from proteins in genetically modified (GM) crops or novel foods for wheat-related proteins. Our database includes evidence-based causative peptides and proteins and two amino acid sequence comparison tools for CeD risk assessment. Sequence entries are based on the review of published studies of specific gluten-reactive T cell activation or intestinal epithelial toxicity. The initial database in 2012 was updated in 2018 and 2022. The current database holds 1,041 causative peptides and 76 representative proteins. The FASTA sequence comparison of 76 representative CeD proteins provides an insurance for possible unreported epitopes. Validation was conducted using protein homologs from Pooideae and non-Pooideae monocots, dicots, and non-plant proteins. Criteria for minimum percent identity and maximum
    Language English
    Publishing date 2022-05-26
    Publishing country Switzerland
    Document type Journal Article
    ISSN 2673-6101
    ISSN (online) 2673-6101
    DOI 10.3389/falgy.2022.900573
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  2. Article ; Online: Correction: Quake et al. Early Introduction of Multi-Allergen Mixture for Prevention of Food Allergy: Pilot Study. Nutrients 2022, 14, 737

    Quake, Antonia Zoe / Liu, Taryn Audrey / D'Souza, Rachel / Jackson, Katherine G / Woch, Margaret / Tetteh, Afua / Sampath, Vanitha / Nadeau, Kari C / Sindher, Sayantani / Chinthrajah, R Sharon / Cao, Shu

    Nutrients

    2022  Volume 15, Issue 1

    Abstract: In the original publication [ ... ]. ...

    Abstract In the original publication [...].
    Language English
    Publishing date 2022-12-28
    Publishing country Switzerland
    Document type Published Erratum
    ZDB-ID 2518386-2
    ISSN 2072-6643 ; 2072-6643
    ISSN (online) 2072-6643
    ISSN 2072-6643
    DOI 10.3390/nu15010135
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  3. Article ; Online: Early Introduction of Multi-Allergen Mixture for Prevention of Food Allergy: Pilot Study.

    Quake, Antonia Zoe / Liu, Taryn Audrey / D'Souza, Rachel / Jackson, Katherine G / Woch, Margaret / Tetteh, Afua / Sampath, Vanitha / Nadeau, Kari C / Sindher, Sayantani / Chinthrajah, R Sharon / Cao, Shu

    Nutrients

    2022  Volume 14, Issue 4

    Abstract: The incidence and prevalence of food allergy (FA) is increasing. While several studies have established the safety and efficacy of early introduction of single allergens in infants for the prevention of FA, the exact dose, frequency, and number of ... ...

    Abstract The incidence and prevalence of food allergy (FA) is increasing. While several studies have established the safety and efficacy of early introduction of single allergens in infants for the prevention of FA, the exact dose, frequency, and number of allergens that can be safely introduced to infants, particularly in those at high or low risk of atopy, are still unclear. This 1-year pilot study evaluated the safety of the early introduction of single foods (milk, egg, or peanut) vs. two foods (milk/egg, egg/peanut, milk/peanut) vs. multiple foods (milk/egg/peanut/cashew/almond/shrimp/walnut/wheat/salmon/hazelnut at low, medium, or high doses) vs. no early introduction in 180 infants between 4-6 months of age. At the end of the study, they were evaluated for plasma biomarkers associated with food reactivity via standardized blood tests. Two to four years after the start of the study, participants were evaluated by standardized food challenges. The serving sizes for the single, double, and low dose mixtures were 300 mg total protein per day. The serving sizes for the medium and high dose mixtures were 900 mg and 3000 mg total protein, respectively. Equal parts of each protein were used for double or mixture foods. All infants were breastfed until at least six months of age. The results demonstrate that infants at either high or low risk for atopy were able to tolerate the early introduction of multiple allergenic foods with no increases in any safety issues, including eczema, FA, or food protein induced enterocolitis. The mixtures of foods at either low, medium, or high doses demonstrated trends for improvement in food challenge reactivity and plasma biomarkers compared to single and double food introductions. The results of this study suggest that the early introduction of foods, particularly simultaneous mixtures of many allergenic foods, may be safe and efficacious for preventing FA and can occur safely. These results need to be confirmed by larger randomized controlled studies.
    MeSH term(s) Allergens ; Arachis ; Food Hypersensitivity/epidemiology ; Food Hypersensitivity/prevention & control ; Humans ; Hypersensitivity, Immediate ; Infant ; Pilot Projects
    Chemical Substances Allergens
    Language English
    Publishing date 2022-02-09
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2518386-2
    ISSN 2072-6643 ; 2072-6643
    ISSN (online) 2072-6643
    ISSN 2072-6643
    DOI 10.3390/nu14040737
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  4. Article: Early Introduction of Multi-Allergen Mixture for Prevention of Food Allergy: Pilot Study

    Quake, Antonia Zoe / Liu, Taryn Audrey / D’Souza, Rachel / Jackson, Katherine G. / Woch, Margaret / Tetteh, Afua / Sampath, Vanitha / Nadeau, Kari C. / Sindher, Sayantani / Chinthrajah, R. Sharon / Cao, Shu

    Nutrients. 2022 Feb. 09, v. 14, no. 4

    2022  

    Abstract: The incidence and prevalence of food allergy (FA) is increasing. While several studies have established the safety and efficacy of early introduction of single allergens in infants for the prevention of FA, the exact dose, frequency, and number of ... ...

    Abstract The incidence and prevalence of food allergy (FA) is increasing. While several studies have established the safety and efficacy of early introduction of single allergens in infants for the prevention of FA, the exact dose, frequency, and number of allergens that can be safely introduced to infants, particularly in those at high or low risk of atopy, are still unclear. This 1-year pilot study evaluated the safety of the early introduction of single foods (milk, egg, or peanut) vs. two foods (milk/egg, egg/peanut, milk/peanut) vs. multiple foods (milk/egg/peanut/cashew/almond/shrimp/walnut/wheat/salmon/hazelnut at low, medium, or high doses) vs. no early introduction in 180 infants between 4–6 months of age. At the end of the study, they were evaluated for plasma biomarkers associated with food reactivity via standardized blood tests. Two to four years after the start of the study, participants were evaluated by standardized food challenges. The serving sizes for the single, double, and low dose mixtures were 300 mg total protein per day. The serving sizes for the medium and high dose mixtures were 900 mg and 3000 mg total protein, respectively. Equal parts of each protein were used for double or mixture foods. All infants were breastfed until at least six months of age. The results demonstrate that infants at either high or low risk for atopy were able to tolerate the early introduction of multiple allergenic foods with no increases in any safety issues, including eczema, FA, or food protein induced enterocolitis. The mixtures of foods at either low, medium, or high doses demonstrated trends for improvement in food challenge reactivity and plasma biomarkers compared to single and double food introductions. The results of this study suggest that the early introduction of foods, particularly simultaneous mixtures of many allergenic foods, may be safe and efficacious for preventing FA and can occur safely. These results need to be confirmed by larger randomized controlled studies.
    Keywords allergenicity ; almonds ; atopy ; biomarkers ; breast feeding ; dietary protein ; eczema ; eggs ; enterocolitis ; food allergies ; hazelnuts ; milk ; peanuts ; protein content ; risk ; salmon ; shrimp ; walnuts ; wheat
    Language English
    Dates of publication 2022-0209
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2518386-2
    ISSN 2072-6643
    ISSN 2072-6643
    DOI 10.3390/nu14040737
    Database NAL-Catalogue (AGRICOLA)

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  5. Article ; Online: Suggested improvements for the allergenicity assessment of genetically modified plants used in foods.

    Goodman, Richard E / Tetteh, Afua O

    Current allergy and asthma reports

    2011  Volume 11, Issue 4, Page(s) 317–324

    Abstract: Genetically modified (GM) plants are increasingly used for food production and industrial applications. As the global population has surpassed 7 billion and per capita consumption rises, food production is challenged by loss of arable land, changing ... ...

    Abstract Genetically modified (GM) plants are increasingly used for food production and industrial applications. As the global population has surpassed 7 billion and per capita consumption rises, food production is challenged by loss of arable land, changing weather patterns, and evolving plant pests and disease. Previous gains in quantity and quality relied on natural or artificial breeding, random mutagenesis, increased pesticide and fertilizer use, and improved farming techniques, all without a formal safety evaluation. However, the direct introduction of novel genes raised questions regarding safety that are being addressed by an evaluation process that considers potential increases in the allergenicity, toxicity, and nutrient availability of foods derived from the GM plants. Opinions vary regarding the adequacy of the assessment, but there is no documented proof of an adverse effect resulting from foods produced from GM plants. This review and opinion discusses current practices and new regulatory demands related to food safety.
    MeSH term(s) Food Hypersensitivity/prevention & control ; Food Safety/methods ; Food, Genetically Modified ; Humans ; Plants, Genetically Modified
    Language English
    Publishing date 2011-04-12
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 2057370-4
    ISSN 1534-6315 ; 1529-7322
    ISSN (online) 1534-6315
    ISSN 1529-7322
    DOI 10.1007/s11882-011-0195-6
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    Zs.A 5548: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  6. Article ; Online: Mass spectrometric analysis of digesta does not improve the allergenicity assessment of GM crops.

    Herman, Rod A / Bauman, Patricia A / Goodwin, Laurie / Islamovic, Emir / Ma, Eric H / Serrano, Hector / Silvanovich, Andre / Simmons, Abigail R / Song, Ping / Tetteh, Afua O / Wang, Rong

    Transgenic research

    2021  Volume 30, Issue 3, Page(s) 283–288

    Abstract: An investigation of the potential allergenicity of newly expressed proteins in genetically modified (GM) crops comprises part of the assessment of GM crop safety. However, allergenicity is not completely predictable from a definitive assay result or set ... ...

    Abstract An investigation of the potential allergenicity of newly expressed proteins in genetically modified (GM) crops comprises part of the assessment of GM crop safety. However, allergenicity is not completely predictable from a definitive assay result or set of protein characteristics, and scientific opinions regarding the data that should be used to assess allergenicity are continuously evolving. Early studies supported a correlation between the stability of a protein exposed to digestive enzymes such as pepsin and the protein's status as a potential allergen, but over time the conclusions of these earlier studies were not confirmed. Nonetheless, many regulatory authorities, including the European Food Safety Authority (EFSA), continue to require digestibility analyses as a component of GM crop risk assessments. Moreover, EFSA has recently investigated the use of mass spectrometry (MS), to make digestion assays more predictive of allergy risk, because it can detect and identify small undigested peptides. However, the utility of MS is questionable in this context, since known allergenic peptides are unlikely to exist in protein candidates intended for commercial development. These protein candidates are pre-screened by the same bioinformatics processes that are normally used to identify MS targets. Therefore, MS is not a standalone allergen identification method and also cannot be used to predict previously unknown allergenic epitopes. Thus, the suggested application of MS for analysis of digesta does not improve the poor predictive power of digestion assays in identifying allergenic risk.
    MeSH term(s) Allergens/adverse effects ; Allergens/immunology ; Allergens/isolation & purification ; Crops, Agricultural/adverse effects ; Crops, Agricultural/chemistry ; Crops, Agricultural/immunology ; Food Safety ; Food, Genetically Modified/adverse effects ; Humans ; Mass Spectrometry ; Plants, Genetically Modified/adverse effects ; Plants, Genetically Modified/chemistry ; Plants, Genetically Modified/immunology
    Chemical Substances Allergens
    Language English
    Publishing date 2021-04-16
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 31620-9
    ISSN 1573-9368 ; 0962-8819
    ISSN (online) 1573-9368
    ISSN 0962-8819
    DOI 10.1007/s11248-021-00254-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Enzymatic Hydrolysis Does Not Reduce the Biological Reactivity of Soybean Proteins for All Allergic Subjects.

    Panda, Rakhi / Tetteh, Afua O / Pramod, Siddanakoppalu N / Goodman, Richard E

    Journal of agricultural and food chemistry

    2015  Volume 63, Issue 43, Page(s) 9629–9639

    Abstract: Many soybean protein products are processed by enzymatic hydrolysis to attain desirable functional food properties or in some cases to reduce allergenicity. However, few studies have investigated the effects of enzymatic hydrolysis on the allergenicity ... ...

    Abstract Many soybean protein products are processed by enzymatic hydrolysis to attain desirable functional food properties or in some cases to reduce allergenicity. However, few studies have investigated the effects of enzymatic hydrolysis on the allergenicity of soybean products. In this study the allergenicity of soybean protein isolates (SPI) hydrolyzed by Alcalase, trypsin, chymotrypsin, bromelain, or papain was evaluated by IgE immunoblots using eight soybean-allergic patient sera. The biological relevance of IgE binding was evaluated by a functional assay using a humanized rat basophilic leukemia (hRBL) cell line and serum from one subject. Results indicated that hydrolysis of SPI by the enzymes did not reduce the allergenicity, and hydrolysis by chymotrypsin or bromelain has the potential to increase the allergenicity of SPI. Two-dimensional (2D) immunoblot and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of the chymotrypsin-hydrolyzed samples indicated fragments of β-conglycinin protein are responsible for the apparent higher allergenic potential of digested SPI.
    MeSH term(s) Chymotrypsin/chemistry ; Food Hypersensitivity/immunology ; Humans ; Hydrolysis ; Immunoglobulin E/analysis ; Immunoglobulin E/immunology ; Soybean Proteins/chemistry ; Soybean Proteins/immunology ; Glycine max/chemistry ; Glycine max/immunology ; Subtilisins/chemistry ; Trypsin/chemistry
    Chemical Substances Soybean Proteins ; Immunoglobulin E (37341-29-0) ; Subtilisins (EC 3.4.21.-) ; Chymotrypsin (EC 3.4.21.1) ; Trypsin (EC 3.4.21.4)
    Language English
    Publishing date 2015-10-20
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 241619-0
    ISSN 1520-5118 ; 0021-8561
    ISSN (online) 1520-5118
    ISSN 0021-8561
    DOI 10.1021/acs.jafc.5b02927
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    Zs.A 1172: Show issues Location:
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  8. Article ; Online: Effectiveness of air purifier on health outcomes and indoor particles in homes of children with allergic diseases in Fresno, California: A pilot study.

    Park, Hye-Kyung / Cheng, Kai-Chung / Tetteh, Afua O / Hildemann, Lynn M / Nadeau, Kari C

    The Journal of asthma : official journal of the Association for the Care of Asthma

    2017  Volume 54, Issue 4, Page(s) 341–346

    Abstract: Objective: Epidemiologic studies indicate that indoor air pollution is correlated with morbidity caused by allergic diseases. We evaluated the effectiveness of reducing the levels of indoor fine particulate matter <2.5 micrometer diameter (PM: Methods! ...

    Abstract Objective: Epidemiologic studies indicate that indoor air pollution is correlated with morbidity caused by allergic diseases. We evaluated the effectiveness of reducing the levels of indoor fine particulate matter <2.5 micrometer diameter (PM
    Methods: The active group (with air purifiers) and the control group consisted of eight houses each. Air purifiers were installed in the living rooms and bedrooms of the subjects in the active group during the entire 12-week study duration. Childhood asthma control test, peak flow rate monitoring, and nasal symptom scores were evaluated at weeks 0, 6, and 12.
    Results: At 12 weeks, the active group showed a trend toward an improvement of childhood asthma control test scores and mean evening peak flow rates, whereas the control group showed deterioration in the same measures. Total and daytime nasal symptoms scores significantly reduced in the active group (p = 0.001 and p = 0.011, respectively). The average indoor PM
    Conclusions: Intervention with air purifiers reduces indoor PM
    MeSH term(s) Adolescent ; Air Filters ; Air Pollution, Indoor/analysis ; Air Pollution, Indoor/prevention & control ; Asthma/therapy ; California ; Child ; Female ; Humans ; Male ; Particulate Matter/analysis ; Pilot Projects ; Rhinitis, Allergic/therapy
    Chemical Substances Particulate Matter
    Language English
    Publishing date 2017-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 603816-5
    ISSN 1532-4303 ; 0277-0903
    ISSN (online) 1532-4303
    ISSN 0277-0903
    DOI 10.1080/02770903.2016.1218011
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.B 18: Show issues Location:
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  9. Article ; Online: Bioinformatics analysis to assess potential risks of allergenicity and toxicity of HRAP and PFLP proteins in genetically modified bananas resistant to Xanthomonas wilt disease.

    Jin, Yuan / Goodman, Richard E / Tetteh, Afua O / Lu, Mei / Tripathi, Leena

    Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

    2017  Volume 109, Issue Pt 1, Page(s) 81–89

    Abstract: Banana Xanthomonas wilt (BXW) disease threatens banana production and food security throughout East Africa. Natural resistance is lacking among common cultivars. Genetically modified (GM) bananas resistant to BXW disease were developed by inserting the ... ...

    Abstract Banana Xanthomonas wilt (BXW) disease threatens banana production and food security throughout East Africa. Natural resistance is lacking among common cultivars. Genetically modified (GM) bananas resistant to BXW disease were developed by inserting the hypersensitive response-assisting protein (Hrap) or/and the plant ferredoxin-like protein (Pflp) gene(s) from sweet pepper (Capsicum annuum). Several of these GM banana events showed 100% resistance to BXW disease under field conditions in Uganda. The current study evaluated the potential allergenicity and toxicity of the expressed proteins HRAP and PFLP based on evaluation of published information on the history of safe use of the natural source of the proteins as well as established bioinformatics sequence comparison methods to known allergens (www.AllergenOnline.org and NCBI Protein) and toxins (NCBI Protein). The results did not identify potential risks of allergy and toxicity to either HRAP or PFLP proteins expressed in the GM bananas that might suggest potential health risks to humans. We recognize that additional tests including stability of these proteins in pepsin assay, nutrient analysis and possibly an acute rodent toxicity assay may be required by national regulatory authorities.
    Language English
    Publishing date 2017-11
    Publishing country England
    Document type Journal Article
    ZDB-ID 782617-5
    ISSN 1873-6351 ; 0278-6915
    ISSN (online) 1873-6351
    ISSN 0278-6915
    DOI 10.1016/j.fct.2017.08.024
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    Zs.A 529: Show issues Location:
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  10. Article: Mass spectrometric analysis of digesta does not improve the allergenicity assessment of GM crops

    Herman, Rod A / Bauman, Patricia A / Goodwin, Laurie / Islamovic, Emir / Ma, Eric H / Serrano, Hector / Silvanovich, Andre / Simmons, Abigail R / Song, Ping / Tetteh, Afua O / Wang, Rong

    Transgenic research. 2021 June, v. 30, no. 3

    2021  

    Abstract: An investigation of the potential allergenicity of newly expressed proteins in genetically modified (GM) crops comprises part of the assessment of GM crop safety. However, allergenicity is not completely predictable from a definitive assay result or set ... ...

    Abstract An investigation of the potential allergenicity of newly expressed proteins in genetically modified (GM) crops comprises part of the assessment of GM crop safety. However, allergenicity is not completely predictable from a definitive assay result or set of protein characteristics, and scientific opinions regarding the data that should be used to assess allergenicity are continuously evolving. Early studies supported a correlation between the stability of a protein exposed to digestive enzymes such as pepsin and the protein’s status as a potential allergen, but over time the conclusions of these earlier studies were not confirmed. Nonetheless, many regulatory authorities, including the European Food Safety Authority (EFSA), continue to require digestibility analyses as a component of GM crop risk assessments. Moreover, EFSA has recently investigated the use of mass spectrometry (MS), to make digestion assays more predictive of allergy risk, because it can detect and identify small undigested peptides. However, the utility of MS is questionable in this context, since known allergenic peptides are unlikely to exist in protein candidates intended for commercial development. These protein candidates are pre-screened by the same bioinformatics processes that are normally used to identify MS targets. Therefore, MS is not a standalone allergen identification method and also cannot be used to predict previously unknown allergenic epitopes. Thus, the suggested application of MS for analysis of digesta does not improve the poor predictive power of digestion assays in identifying allergenic risk.
    Keywords allergenicity ; allergens ; bioinformatics ; crop safety ; digesta ; digestibility ; digestion ; epitopes ; food safety ; genetic engineering ; hypersensitivity ; mass spectrometry ; pepsin ; peptides ; risk
    Language English
    Dates of publication 2021-06
    Size p. 283-288.
    Publishing place Springer International Publishing
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 31620-9
    ISSN 1573-9368 ; 0962-8819
    ISSN (online) 1573-9368
    ISSN 0962-8819
    DOI 10.1007/s11248-021-00254-x
    Database NAL-Catalogue (AGRICOLA)

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