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Book ; Thesis: Measurement of the jet energy resolution at the CMS experiment in top-quark pair events

Tews, Alexandra

Messung der Jet-Energieauflösung des CMS Experiments in Top Quark Paar Zerfällen

2014

Author's details	vorgelegt von Alexandra Tews
Language	English
Size	104 S., graph. Darst.
Document type	Book ; Thesis
Thesis / German Habilitation thesis	Univ., FB Physik, Bachelorarbeit--Hamburg, 2014
Accompanying material	1 CD-ROM (12 cm)
Database	Former special subject collection: coastal and deep sea fishing

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Article ; Online: What is the best way to keep the patient warm during technical rescue? Results from two prospective randomised controlled studies with healthy volunteers.

Lier, Martin / Jebens, Christopher / Lorey-Tews, Annette / Heyne, Tim / Kunze-Szikszay, Nils / Wieditz, Johannes / Bräuer, Anselm

BMC emergency medicine

2023 Volume 23, Issue 1, Page(s) 83

Abstract: Background: Accidental hypothermia is a manifest problem during the rescue of entrapped victims and results in different subsequent problems as coagulopathy and wound infection. Different warming methods are available for the preclinicial use. However, ... ...

Abstract	Background: Accidental hypothermia is a manifest problem during the rescue of entrapped victims and results in different subsequent problems as coagulopathy and wound infection. Different warming methods are available for the preclinicial use. However, their effectiveness has hardly been evaluated. Methods: In a first step a survey among German fire brigades was performed with questions about the most used warming methods. In a second step two crossover studies were conducted. In each study two different warming method were compared with forced air warming - which is the most frequently used and highly effective warming method in operation rooms (Study A: halogen floodlight vs. forced air warming; Study B: forced air warming vs. fleece blanket). In both studies healthy volunteers (Study A: 30 volunteers, Study B: 32 volunteers) were sitting 60 min in a cold store. In the first 21 min there was no subject warming. Afterwards the different warming methods were initiated. Every 3 min parameters like skin temperature, core body temperature and cold perception on a 10-point numeric rating scale were recorded. Linear mixed models were fitted for each parameter to check for differences in temperature trajectories and cold perception with regard to the different warming methods. Results: One hundred fifty-one German fire brigades responded to the survey. The most frequently used warming methods were different rescue blankets (gold/silver, wool) and work light (halogen floodlights). Both studies (A and B) showed significantly (p < 0.05) higher values in mean skin temperature, mean body temperature and total body heat for the forced air warming methods compared to halogen floodlight respectively fleece blanket shortly after warming initiation. In contrast, values for the cold perception were significantly lower (less unpleasant cold perception) during the phase the forced air warming methods were used, compared to the fleece blanket or the halogen floodlight was used. Conclusion: Forced air warming methods used under the standardised experimental setting are an effective method to keep patients warm during technical rescue. Halogen floodlight has an insufficient effect on the patient's heat preservation. In healthy subjects, fleece blankets will stop heat loss but will not correct heat that has already been lost. Trial registration: The studies were registered retrospectively on 14/02/2022 on the German Clinical Trials registry (DRKS) with the number DRKS00028079.
MeSH term(s)	Humans ; Body Temperature Regulation ; Healthy Volunteers ; Hypothermia/prevention & control ; Prospective Studies ; Retrospective Studies ; Cross-Over Studies
Language	English
Publishing date	2023-08-04
Publishing country	England
Document type	Journal Article ; Randomized Controlled Trial
ZDB-ID	2050431-7
ISSN	1471-227X ; 1471-227X
ISSN (online)	1471-227X
ISSN	1471-227X
DOI	10.1186/s12873-023-00850-6
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Article ; Online: The power of odour cues in shaping fine-scale search patterns of foraging mammalian herbivores.

Orlando, Cristian Gabriel / Tews, Ashley / Banks, Peter / McArthur, Clare

Biology letters

2020 Volume 16, Issue 7, Page(s) 20200329

Abstract: Foraging by mammalian herbivores has profound impacts on natural and modified landscapes, yet we know little about how they find food, limiting our ability to predict and manage their influence. Mathematical models show that foragers exploiting odour ... ...

Abstract	Foraging by mammalian herbivores has profound impacts on natural and modified landscapes, yet we know little about how they find food, limiting our ability to predict and manage their influence. Mathematical models show that foragers exploiting odour cues outperform a random walk strategy. However, discovering how free-ranging foragers exploit odours in real, complex landscapes has proven elusive because of technological constraints. We took a novel approach, using a sophisticated purpose-built thermal camera system to record fine-scale foraging by a generalist mammalian herbivore, the swamp wallaby (
MeSH term(s)	Animals ; Cues ; Feeding Behavior ; Food ; Herbivory ; Macropodidae ; Odorants
Language	English
Publishing date	2020-07-15
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2135022-X
ISSN	1744-957X ; 1744-9561
ISSN (online)	1744-957X
ISSN	1744-9561
DOI	10.1098/rsbl.2020.0329
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Article ; Online: A double role of the Gal80 N terminus in activation of transcription by Gal4p.

Reinhardt-Tews, Annekathrin / Krutyhołowa, Rościsław / Günzel, Christian / Roehl, Constance / Glatt, Sebastian / Breunig, Karin D

Life science alliance

2020 Volume 3, Issue 12

Abstract: The yeast galactose switch operated by the Gal4p-Gal80p-Gal3p regulatory module is a textbook model of transcription regulation in eukaryotes. The Gal80 protein inhibits Gal4p-mediated transcription activation by binding to the transcription activation ... ...

Abstract	The yeast galactose switch operated by the Gal4p-Gal80p-Gal3p regulatory module is a textbook model of transcription regulation in eukaryotes. The Gal80 protein inhibits Gal4p-mediated transcription activation by binding to the transcription activation domain. In
MeSH term(s)	Cell Nucleus/metabolism ; DNA-Binding Proteins/metabolism ; Galactokinase/genetics ; Galactose/metabolism ; Kluyveromyces/genetics ; Repressor Proteins/metabolism ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; Saccharomyces cerevisiae Proteins/metabolism ; Transcription Factors/metabolism ; Transcriptional Activation
Chemical Substances	DNA-Binding Proteins ; GAL4 protein, S cerevisiae ; GAL80 protein, S cerevisiae ; Gal3 protein, S cerevisiae ; Repressor Proteins ; Saccharomyces cerevisiae Proteins ; Transcription Factors ; Galactokinase (EC 2.7.1.6) ; Galactose (X2RN3Q8DNE)
Language	English
Publishing date	2020-10-09
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2575-1077
ISSN (online)	2575-1077
DOI	10.26508/lsa.202000665
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Article ; Online: Fungal Kti12 proteins display unusual linker regions and unique ATPase p-loops.

Krutyhołowa, Rościsław / Reinhardt-Tews, Annekathrin / Chramiec-Głąbik, Andrzej / Breunig, Karin D / Glatt, Sebastian

Current genetics

2020 Volume 66, Issue 4, Page(s) 823–833

Abstract: Kti12 (Kluyveromyces lactis toxin insensitive 12) is an evolutionary highly conserved ATPase, crucial for the tRNA-modification activity of the eukaryotic Elongator complex. The protein consists of an N-terminal ATPase and a C-terminal tRNA-binding ... ...

Abstract	Kti12 (Kluyveromyces lactis toxin insensitive 12) is an evolutionary highly conserved ATPase, crucial for the tRNA-modification activity of the eukaryotic Elongator complex. The protein consists of an N-terminal ATPase and a C-terminal tRNA-binding domain, which are connected by a flexible linker. The precise role of the linker region and its involvement in the communication between the two domains and their activities remain elusive. Here, we analyzed all available Kti12 protein sequences and report the discovery of a subset of Kti12 proteins with abnormally long linker regions. These Kti12 proteins are characterized by a co-occurring lysine to leucine substitution in their Walker A motif, previously thought to be invariable. We show that the K14L substitution lowers the affinity to ATP, but does not affect the catalytic activity of Kti12 at high ATP concentrations. We compare the activity of mutated variants of Kti12 in vitro with complementation assays in vivo in yeast. Ultimately, we compared Kti12 to other known p-loop ATPase family members known to carry a similar deviant Walker A motif. Our data establish Kti12 of Eurotiomycetes as an example of eukaryotic ATPase harboring a significantly deviating but still functional Walker A motif.
MeSH term(s)	AAA Domain ; Adaptor Proteins, Signal Transducing/chemistry ; Adaptor Proteins, Signal Transducing/genetics ; Adaptor Proteins, Signal Transducing/metabolism ; Amino Acid Substitution ; Animals ; Catalytic Domain ; Evolution, Molecular ; Fungal Proteins/chemistry ; Fungal Proteins/genetics ; Fungal Proteins/metabolism ; Killer Factors, Yeast/pharmacology ; Kluyveromyces/metabolism ; Lysine/chemistry ; Machine Learning ; Models, Molecular ; Mutation ; Protein Conformation ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; Saccharomyces cerevisiae Proteins/chemistry ; Saccharomyces cerevisiae Proteins/genetics ; Saccharomyces cerevisiae Proteins/metabolism
Chemical Substances	Adaptor Proteins, Signal Transducing ; Fungal Proteins ; KTI12 protein, S cerevisiae ; Killer Factors, Yeast ; Saccharomyces cerevisiae Proteins ; zymocin ; Lysine (K3Z4F929H6)
Language	English
Publishing date	2020-03-31
Publishing country	United States
Document type	Journal Article
ZDB-ID	282876-5
ISSN	1432-0983 ; 0172-8083
ISSN (online)	1432-0983
ISSN	0172-8083
DOI	10.1007/s00294-020-01070-2
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Article: Der Clonidin-Hemmtest: eine Hilfe in der Diagnostik und postoperativen Therapiekontrolle beim Phäochromozytom

Schofer, J. / Tews, A.

DMW - Deutsche Medizinische Wochenschrift

2008 Volume 110, Issue 37, Page(s) 1403–1407

Abstract: Bei acht Patienten mit arteriellem Hypertonus, erhöhten Urin- und Plasma-Katecholaminwerten und einer auf Phäochromozytom verdächtigen Symptomatik wurde ein Clonidin-Hemmtest durchgeführt. Bei sechs von ihnen ließen sich die Plasma-Katecholamine durch ... ...

Abstract	Bei acht Patienten mit arteriellem Hypertonus, erhöhten Urin- und Plasma-Katecholaminwerten und einer auf Phäochromozytom verdächtigen Symptomatik wurde ein Clonidin-Hemmtest durchgeführt. Bei sechs von ihnen ließen sich die Plasma-Katecholamine durch Clonidin senken; sie wiesen ein unauffälliges abdominales Computertomogramm (CT) auf. Bei zwei Patienten fanden sich exzessiv erhöhte Noradrenalinwerte, die durch Clonidin nicht zu senken waren; bei einem von ihnen war auch die Adrenalin-Plasmakonzentration erhöht und konnte ebenfalls nicht durch Clonidin gesenkt werden. Bei diesen beiden Patienten war im CT ein Tumor nachweisbar, der beim einen in der linken Nebenniere, beim anderen extramedullär gelegen war. Operativ fand sich in beiden Fällen ein Phäochromozytom. Im Fall des extramedullär gelegenen Tumors wurden intraoperativ Peritoneal- und Lebermetastasen entdeckt. Postoperativ wurde der Clonidin-Hemmtest bei beiden Patienten wiederholt: Er war im Fall des intramedullär gelegenen, vollständig entfernten Phäochromozytoms normal, im anderen Fall weiter pathologisch. Demnach erweist sich der Clonidin-Hemmtest in der Diagnostik des Phäochromozytoms und zur Überprüfung des Operationserfolges als sinnvoll.
Language	German
Publishing date	2008-03-25
Publishing place	Stuttgart ; New York
Document type	Article
ZDB-ID	200446-x
ISSN	1439-4413 ; 0012-0472
ISSN (online)	1439-4413
ISSN	0012-0472
DOI	10.1055/s-2008-1069018
Database	Thieme publisher's database

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Article ; Online: Kti12, a PSTK-like tRNA dependent ATPase essential for tRNA modification by Elongator.

Krutyhołowa, Rościsław / Hammermeister, Alexander / Zabel, Rene / Abdel-Fattah, Wael / Reinhardt-Tews, Annekathrin / Helm, Mark / Stark, Michael J R / Breunig, Karin D / Schaffrath, Raffael / Glatt, Sebastian

Nucleic acids research

2019 Volume 47, Issue 9, Page(s) 4814–4830

Abstract: Posttranscriptional RNA modifications occur in all domains of life. Modifications of anticodon bases are of particular importance for ribosomal decoding and proteome homeostasis. The Elongator complex modifies uridines in the wobble position and is ... ...

Abstract	Posttranscriptional RNA modifications occur in all domains of life. Modifications of anticodon bases are of particular importance for ribosomal decoding and proteome homeostasis. The Elongator complex modifies uridines in the wobble position and is highly conserved in eukaryotes. Despite recent insights into Elongator's architecture, the structure and function of its regulatory factor Kti12 have remained elusive. Here, we present the crystal structure of Kti12's nucleotide hydrolase domain trapped in a transition state of ATP hydrolysis. The structure reveals striking similarities to an O-phosphoseryl-tRNA kinase involved in the selenocysteine pathway. Both proteins employ similar mechanisms of tRNA binding and show tRNASec-dependent ATPase activity. In addition, we demonstrate that Kti12 binds directly to Elongator and that ATP hydrolysis is crucial for Elongator to maintain proper tRNA anticodon modification levels in vivo. In summary, our data reveal a hitherto uncharacterized link between two translational control pathways that regulate selenocysteine incorporation and affect ribosomal tRNA selection via specific tRNA modifications.
MeSH term(s)	Adaptor Proteins, Signal Transducing/chemistry ; Adaptor Proteins, Signal Transducing/genetics ; Adenosine Triphosphatases/chemistry ; Adenosine Triphosphatases/genetics ; Anticodon/genetics ; Carrier Proteins/chemistry ; Carrier Proteins/genetics ; Chaetomium/chemistry ; Chaetomium/enzymology ; Crystallography, X-Ray ; Protein Conformation ; RNA Processing, Post-Transcriptional/genetics ; RNA, Transfer/genetics ; Ribosomes/genetics ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae Proteins/chemistry ; Saccharomyces cerevisiae Proteins/genetics ; Uridine/genetics
Chemical Substances	Adaptor Proteins, Signal Transducing ; Anticodon ; Carrier Proteins ; KTI12 protein, S cerevisiae ; Saccharomyces cerevisiae Proteins ; RNA, Transfer (9014-25-9) ; Adenosine Triphosphatases (EC 3.6.1.-) ; Uridine (WHI7HQ7H85)
Language	English
Publishing date	2019-03-11
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	186809-3
ISSN	1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048
ISSN (online)	1362-4962 ; 1362-4954
ISSN	0301-5610 ; 0305-1048
DOI	10.1093/nar/gkz190
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Article ; Online: Autonomous surveillance for biosecurity.

Jurdak, Raja / Elfes, Alberto / Kusy, Branislav / Tews, Ashley / Hu, Wen / Hernandez, Emili / Kottege, Navinda / Sikka, Pavan

Trends in biotechnology

2015 Volume 33, Issue 4, Page(s) 201–207

Abstract: The global movement of people and goods has increased the risk of biosecurity threats and their potential to incur large economic, social, and environmental costs. Conventional manual biosecurity surveillance methods are limited by their scalability in ... ...

Abstract	The global movement of people and goods has increased the risk of biosecurity threats and their potential to incur large economic, social, and environmental costs. Conventional manual biosecurity surveillance methods are limited by their scalability in space and time. This article focuses on autonomous surveillance systems, comprising sensor networks, robots, and intelligent algorithms, and their applicability to biosecurity threats. We discuss the spatial and temporal attributes of autonomous surveillance technologies and map them to three broad categories of biosecurity threat: (i) vector-borne diseases; (ii) plant pests; and (iii) aquatic pests. Our discussion reveals a broad range of opportunities to serve biosecurity needs through autonomous surveillance.
MeSH term(s)	Crops, Agricultural ; Environmental Monitoring/instrumentation ; Robotics/instrumentation ; Security Measures
Language	English
Publishing date	2015-04
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	47474-5
ISSN	1879-3096 ; 0167-7799
ISSN (online)	1879-3096
ISSN	0167-7799
DOI	10.1016/j.tibtech.2015.01.003
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Article ; Online: An ancient oxidoreductase making differential use of its cofactors.

Blüher, Doreen / Reinhardt-Tews, Annekathrin / Hey, Martin / Lilie, Hauke / Golbik, Ralph / Breunig, Karin D / Anders, Alexander

Biological chemistry

2014 Volume 395, Issue 7-8, Page(s) 855–869

Abstract: Abstract Many transcription factors contribute to cellular homeostasis by integrating multiple signals. Signaling via the yeast Gal80 protein, a negative regulator of the prototypic transcription activator Gal4, is primarily regulated by galactose. ... ...

Abstract	Abstract Many transcription factors contribute to cellular homeostasis by integrating multiple signals. Signaling via the yeast Gal80 protein, a negative regulator of the prototypic transcription activator Gal4, is primarily regulated by galactose. ScGal80 from Saccharomyces cerevisiae has been reported to bind NAD(P). Here, we show that the ability to bind these ligands is conserved in KlGal80, a Gal80 homolog from the distantly related yeast Kluyveromyces lactis. However, the homologs apparently have diverged with respect to response to the dinucleotide. Strikingly, ScGal80 binds NAD(P) and NAD(P)H with more than 50-fold higher affinity than KlGal80. In contrast to ScGal80, where NAD is neutral, NAD and NADP have a negative effect in KlGal80 on its interaction with a KlGal4-peptide in vitro. Swapping a loop in the NAD(P) binding Rossmann-fold of ScGal80 into KlGal80 increases the affinity for NAD(P) and has a significant impact on KlGal4 regulation in vivo. Apparently, dinucleotide binding allows coupling of the metabolic state of the cell to regulation of the GAL/LAC genes. The particular sequences involved in binding determine how exactly the metabolic state is sensed and integrated by Gal80 to regulate Gal4.
MeSH term(s)	Coenzymes/genetics ; Coenzymes/metabolism ; Fungal Proteins/genetics ; Fungal Proteins/metabolism ; Galactose/metabolism ; Ligands ; NAD/metabolism ; Oxidoreductases/genetics ; Oxidoreductases/metabolism
Chemical Substances	Coenzymes ; Fungal Proteins ; Ligands ; NAD (0U46U6E8UK) ; Oxidoreductases (EC 1.-) ; Galactose (X2RN3Q8DNE)
Language	English
Publishing date	2014-07
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1334659-3
ISSN	1437-4315 ; 1431-6730 ; 1432-0355
ISSN (online)	1437-4315
ISSN	1431-6730 ; 1432-0355
DOI	10.1515/hsz-2014-0152
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Article: Der Clonidin-Hemmtest: eine Hilfe in der Diagnostik und postoperativen Therapiekontrolle beim Phäochromozytom.

Schofer, J / Tews, A

Deutsche medizinische Wochenschrift (1946)

1985 Volume 110, Issue 37, Page(s) 1403–1407

Abstract: A clonidine-suppression test was carried out in 8 patients with arterial hypertension, raised urinary and plasma catecholamine levels and symptoms suggesting the presence of a pheochromocytoma. In 6 of the patients clonidine reduced the plasma ... ...

Title translation	The clonidine inhibition test: an aid in the diagnosis and postoperative therapeutic control in pheochromocytoma.
Abstract	A clonidine-suppression test was carried out in 8 patients with arterial hypertension, raised urinary and plasma catecholamine levels and symptoms suggesting the presence of a pheochromocytoma. In 6 of the patients clonidine reduced the plasma catecholamine concentrations and no pathological findings were seen in the abdominal computed tomogram (CT). In 2 patients noradrenaline levels were excessively high and not lowered by clonidine; in one of these patients the plasma adrenaline concentration was also raised and this too could not be reduced with clonidine. A tumor was detectable in both patients using CT, in one case situated in the left adrenal, in the other extra-medullary. On operation both were found to be a pheochromocytoma. Peritoneal and intra-hepatic metastases were discovered during surgery in the case of the extramedullary localized tumor. The clonidine-suppression test was repeated postoperatively in both patients: it was normal in the case of the completely excised, intramedullary situated tumor but still pathological in the other. Thus the clonidine-suppression test is useful in the diagnosis and post-surgical assessment of pheochromocytoma.
MeSH term(s)	Adult ; Blood Pressure Determination ; Clonidine ; Epinephrine/blood ; Female ; Humans ; Liver Neoplasms/secondary ; Male ; Middle Aged ; Norepinephrine/blood ; Peritoneal Neoplasms/secondary ; Pheochromocytoma/diagnosis ; Radiography, Abdominal ; Tomography, X-Ray Computed
Chemical Substances	Clonidine (MN3L5RMN02) ; Norepinephrine (X4W3ENH1CV) ; Epinephrine (YKH834O4BH)
Language	German
Publishing date	1985-09-13
Publishing country	Germany
Document type	English Abstract ; Journal Article
ZDB-ID	200446-x
ISSN	1439-4413 ; 0012-0472
ISSN (online)	1439-4413
ISSN	0012-0472
DOI	10.1055/s-2008-1069018
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