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  1. Article ; Online: Exposure to submicron particles (PM1.0) from diesel exhaust and pollen allergens of human lung epithelial cells induces morphological changes of mitochondria tonifilaments and rough endoplasmic reticulum.

    Mazzarella, Gennaro / Lucariello, Angela / Bianco, Andrea / Calabrese, Cecilia / Thanassoulas, Theodoros / Savarese, Leonilde / Fiumarella, Angelamaria / Esposito, Vincenzo / DE Luca, Antonio

    In vivo (Athens, Greece)

    2014  Volume 28, Issue 4, Page(s) 557–561

    Abstract: In recent literature, little has been said regarding the morphological changes that occur in lung cells after treatment with particles and nanoparticles. Using an in vitro model of type-II lung epithelium (A549), we studied the effects of submicron ... ...

    Abstract In recent literature, little has been said regarding the morphological changes that occur in lung cells after treatment with particles and nanoparticles. Using an in vitro model of type-II lung epithelium (A549), we studied the effects of submicron particles (PM1.0), Parietaria officinalis (ALL), and PM1.0 + ALL together. To date several biochemical effects have been described, instead few data exist in literature regarding morphological events following these treatments, in particular we focused on the morphological changes and distribution of mitochondria, tonifilaments and rough endoplasmic reticulum, using a transmission electron microscopic (TEM) approach. After exposure to PM1.0 particles (PM1.0), Parietaria officinalis as allergen, and PM1.0 with P. officinalis, changes in the cytoplasmic area were observed, such as damage to mitochondria and morphological alterations of the tonifilaments and rough endoplasmic reticulum. The data obtained strongly support the hypothesis that cells in contact with submicron particles (PM1.0), or P. officinalis, undergo alteration of their metabolism.
    MeSH term(s) Allergens/adverse effects ; Allergens/toxicity ; Cell Line, Tumor ; Cells, Cultured ; Endoplasmic Reticulum, Rough/ultrastructure ; Epithelial Cells/drug effects ; Epithelial Cells/pathology ; Epithelial Cells/ultrastructure ; Humans ; Mitochondria/ultrastructure ; Particulate Matter/toxicity ; Pollen/adverse effects ; Pollen/toxicity ; Vehicle Emissions/toxicity
    Chemical Substances Allergens ; Particulate Matter ; Vehicle Emissions
    Language English
    Publishing date 2014-07
    Publishing country Greece
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 807031-3
    ISSN 1791-7549 ; 0258-851X
    ISSN (online) 1791-7549
    ISSN 0258-851X
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Preamplification procedure for the analysis of ancient DNA samples.

    Del Gaudio, Stefania / Cirillo, Alessandra / Di Bernardo, Giovanni / Galderisi, Umberto / Thanassoulas, Theodoros / Pitsios, Theodoros / Cipollaro, Marilena

    TheScientificWorldJournal

    2013  Volume 2013, Page(s) 734676

    Abstract: In ancient DNA studies the low amount of endogenous DNA represents a limiting factor that often hampers the result achievement. In this study we extracted the DNA from nine human skeletal remains of different ages found in the Byzantine cemetery of ... ...

    Abstract In ancient DNA studies the low amount of endogenous DNA represents a limiting factor that often hampers the result achievement. In this study we extracted the DNA from nine human skeletal remains of different ages found in the Byzantine cemetery of Abdera Halkidiki and in the medieval cemetery of St. Spiridion in Rhodes (Greece). Real-time quantitative polymerase chain reaction (qPCR) was used to detect in the extracts the presence of PCR inhibitors and to estimate the DNA content. As mitochondrial DNA was detected in all samples, amplification of nuclear targets, as amelogenin and the polymorphism M470V of the transmembrane conductance regulator gene, yielded positive results in one case only. In an effort to improve amplification success, we applied, for the first time in ancient DNA, a preamplification strategy based on TaqMan PreAmp Master Mix. A comparison between results obtained from nonpreamplified and preamplified samples is reported. Our data, even if preliminary, show that the TaqMan PreAmp procedure may improve the sensitivity of qPCR analysis.
    MeSH term(s) Anthropology, Physical/methods ; Base Sequence ; DNA/genetics ; DNA Fingerprinting/methods ; Forensic Genetics/methods ; Greece ; Molecular Sequence Data ; Real-Time Polymerase Chain Reaction/methods ; Sequence Analysis, DNA/methods ; Specimen Handling/methods
    Chemical Substances DNA (9007-49-2)
    Language English
    Publishing date 2013-09-25
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2075968-X
    ISSN 1537-744X ; 1537-744X
    ISSN (online) 1537-744X
    ISSN 1537-744X
    DOI 10.1155/2013/734676
    Database MEDical Literature Analysis and Retrieval System OnLINE

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