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  1. Article ; Online: Pathology in Practice.

    Thebeau, Jenna M / Martinson, Shannon A / Clancey, Noel P / Raab, Oriana D

    Journal of the American Veterinary Medical Association

    2022  Volume 259, Issue S2, Page(s) 1–4

    Abstract: In collaboration with the American College of Veterinary Pathologists. ...

    Abstract In collaboration with the American College of Veterinary Pathologists.
    MeSH term(s) Animals ; Humans ; Pathology, Veterinary ; United States ; Veterinarians
    Language English
    Publishing date 2022-05-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 390811-2
    ISSN 1943-569X ; 0003-1488
    ISSN (online) 1943-569X
    ISSN 0003-1488
    DOI 10.2460/javma.21.02.0077
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Fall Treatment with Fumagillin Contributes to an Overwinter Shift in

    Biganski, Sarah / Obshta, Oleksii / Kozii, Ivanna / Koziy, Roman / Zabrodski, Michael W / Jose, Midhun S / Thebeau, Jenna M / Silva, Marina C B / Raza, Muhammad F / Masood, Fatima / Wood, Sarah C / Simko, Elemir

    Life (Basel, Switzerland)

    2024  Volume 14, Issue 3

    Abstract: 1) Background: Microsporidiosis (nosemosis) is an intestinal disorder of adult honey bees caused by the microsporidian ... ...

    Abstract (1) Background: Microsporidiosis (nosemosis) is an intestinal disorder of adult honey bees caused by the microsporidian pathogens
    Language English
    Publishing date 2024-03-12
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662250-6
    ISSN 2075-1729
    ISSN 2075-1729
    DOI 10.3390/life14030373
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Fetal Alcohol Spectrum Disorder: The Honey Bee as a Social Animal Model.

    Camilli, Marcelo P / Simko, Olena M / Bevelander, Breanne / Thebeau, Jenna M / Masood, Fatima / da Silva, Marina C Bezerra / Raza, Muhammad Fahim / Markova, Sofiia / Obshta, Oleksii / Jose, Midhun S / Biganski, Sarah / Kozii, Ivanna V / Zabrodski, Michael W / Moshynskyy, Igor / Simko, Elemir / Wood, Sarah C

    Life (Basel, Switzerland)

    2024  Volume 14, Issue 4

    Abstract: Animal models have been essential for advancing research of fetal alcohol spectrum disorder (FASD) in humans, but few animal species effectively replicate the behavioural and clinical signs of FASD. The honey bee ( ...

    Abstract Animal models have been essential for advancing research of fetal alcohol spectrum disorder (FASD) in humans, but few animal species effectively replicate the behavioural and clinical signs of FASD. The honey bee (
    Language English
    Publishing date 2024-03-25
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662250-6
    ISSN 2075-1729
    ISSN 2075-1729
    DOI 10.3390/life14040434
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Comparison of individual and pooled sampling methods for estimation of Vairimorpha (Nosema) spp. levels in experimentally infected honey bee colonies

    Biganski, Sarah / Lester, Tessa / Obshta, Oleksii / Jose, Midhun S. / Thebeau, Jenna M. / Masood, Fatima / Silva, Marina C. B. / Camilli, Marcelo P. / Raza, Muhammad F. / Zabrodski, Michael W. / Kozii, Ivanna / Koziy, Roman / Moshynskyy, Igor / Simko, Elemir / Wood, Sarah C.

    Journal of Veterinary Diagnostic Investigation. 2023 Nov., v. 35, no. 6 p.639-644

    2023  

    Abstract: The microsporidian pathogens Vairimorpha apis and V. ceranae are known to cause intestinal infection in honey bees and are associated with decreased colony productivity and colony loss. The widely accepted method for determining Vairimorpha colony ... ...

    Abstract The microsporidian pathogens Vairimorpha apis and V. ceranae are known to cause intestinal infection in honey bees and are associated with decreased colony productivity and colony loss. The widely accepted method for determining Vairimorpha colony infection level for risk assessment and antibiotic treatment is based on spore counts of 60 pooled worker bees using light microscopy. Given that honey bee colonies consist of as many as 1,000 times more individuals, the number of bees collected for Vairimorpha detection may significantly impact the estimated colony infection level, especially in the case of uneven distribution of high- and low-infected individuals within a hive. Hence, we compared the frequency and severity of Vairimorpha infection in individual bees to pooled samples of 60, 120, and 180 bees, as well as compared the Vairimorpha spp. prevalence in pooled samples of 60 and 180 bees. Overall, we did not find significant differences in spore counts in pooled samples containing incremental numbers of bees, although we observed that, in less-infected colonies, a low frequency of highly infected individuals influenced the estimated colony infection level. Moreover, Vairimorpha spp. prevalence did not differ significantly among the pooled bee samples tested. Increasing the number of pooled bees from the recommended 60 bees to 180 bees did not yield a more accurate representation of colony infection level for highly infected colonies, but the clinical importance of a low frequency of highly infected individuals in less-infected colonies needs to be addressed in future studies.
    Keywords Nosema ; Vairimorpha ; antibiotics ; gastrointestinal diseases ; honey ; honey bees ; light microscopy ; risk assessment ; spores ; colony infection ; sampling methods ; Vairimorpha apis ; Vairimorpha ceranae
    Language English
    Dates of publication 2023-11
    Size p. 639-644.
    Publishing place SAGE Publications
    Document type Article ; Online
    ZDB-ID 287603-6
    ISSN 1943-4936 ; 1040-6387
    ISSN (online) 1943-4936
    ISSN 1040-6387
    DOI 10.1177/10406387231194620
    Database NAL-Catalogue (AGRICOLA)

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  5. Article ; Online: Comparison of individual and pooled sampling methods for estimation of

    Biganski, Sarah / Lester, Tessa / Obshta, Oleksii / Jose, Midhun S / Thebeau, Jenna M / Masood, Fatima / Silva, Marina C B / Camilli, Marcelo P / Raza, Muhammad F / Zabrodski, Michael W / Kozii, Ivanna / Koziy, Roman / Moshynskyy, Igor / Simko, Elemir / Wood, Sarah C

    Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc

    2023  Volume 35, Issue 6, Page(s) 639–644

    Abstract: The microsporidian ... ...

    Abstract The microsporidian pathogens
    MeSH term(s) Bees ; Animals ; Nosema ; Prevalence ; Seasons
    Language English
    Publishing date 2023-08-28
    Publishing country United States
    Document type Journal Article
    ZDB-ID 287603-6
    ISSN 1943-4936 ; 1040-6387
    ISSN (online) 1943-4936
    ISSN 1040-6387
    DOI 10.1177/10406387231194620
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Evaluating approved and alternative treatments against an oxytetracycline-resistant bacterium responsible for European foulbrood disease in honey bees.

    Masood, Fatima / Thebeau, Jenna M / Cloet, Allyssa / Kozii, Ivanna V / Zabrodski, Michael W / Biganski, Sarah / Liang, Jenny / Marta Guarna, M / Simko, Elemir / Ruzzini, Antonio / Wood, Sarah C

    Scientific reports

    2022  Volume 12, Issue 1, Page(s) 5906

    Abstract: European foulbrood (EFB) is a disease of honey bee larvae caused by Melissococcus plutonius. In North America, oxytetracycline (OTC) is approved to combat EFB disease though tylosin (TYL) and lincomycin (LMC) are also registered for use against American ... ...

    Abstract European foulbrood (EFB) is a disease of honey bee larvae caused by Melissococcus plutonius. In North America, oxytetracycline (OTC) is approved to combat EFB disease though tylosin (TYL) and lincomycin (LMC) are also registered for use against American foulbrood disease. Herein, we report and characterize an OTC-resistant M. plutonius isolate from British Columbia, Canada, providing an antimicrobial sensitivity to the three approved antibiotics and studying their abilities to alter larval survival in an in vitro infection model. Specifically, we investigated OTC, TYL, and LMC as potential treatment options for EFB disease using laboratory-reared larvae infected with M. plutonius. The utility of the three antibiotics were compared through an experimental design that either mimicked metaphylaxis or antimicrobial intervention. At varying concentrations, all three antibiotics prevented clinical signs of EFB disease following infection with M. plutonius 2019BC1 in vitro. This included treatment with 100 μg/mL of OTC, a concentration that was ~ 3× the minimum inhibitory concentration measured to inhibit the strain in nutrient broth. Additionally, we noted high larval mortality in groups treated with doses of OTC corresponding to ~ 30× the dose required to eliminate bacterial growth in vitro. In contrast, TYL and LMC were not toxic to larvae at concentrations that exceed field use. As we continue to investigate antimicrobial resistance (AMR) profiles of M. plutonius from known EFB outbreaks, we expect a range of AMR phenotypes, reiterating the importance of expanding current therapeutic options along with alternative management practices to suppress this disease.
    MeSH term(s) Animals ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Bacteria ; Bees ; British Columbia ; Larva ; Oxytetracycline/pharmacology ; Oxytetracycline/therapeutic use ; United States
    Chemical Substances Anti-Bacterial Agents ; Oxytetracycline (X20I9EN955)
    Language English
    Publishing date 2022-04-07
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-022-09796-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Investigation of

    Thebeau, Jenna M / Liebe, Dana / Masood, Fatima / Kozii, Ivanna V / Klein, Colby D / Zabrodski, Michael W / Moshynskyy, Igor / Sobchishin, Larhonda / Wilson, Geoff / Guarna, M Marta / Gerbrandt, Eric M / Simko, Elemir / Wood, Sarah C

    The Canadian veterinary journal = La revue veterinaire canadienne

    2022  Volume 63, Issue 9, Page(s) 935–942

    Abstract: European foulbrood (EFB) disease is an economically important bacterial disease of honey bee larvae caused by enteric infection ... ...

    Abstract European foulbrood (EFB) disease is an economically important bacterial disease of honey bee larvae caused by enteric infection with
    MeSH term(s) Alberta ; Animals ; Beekeeping ; Bees ; Disease Outbreaks/veterinary ; Enterococcaceae/genetics ; Larva/microbiology
    Language English
    Publishing date 2022-09-05
    Publishing country Canada
    Document type Journal Article
    ZDB-ID 41603-4
    ISSN 0008-5286
    ISSN 0008-5286
    Database MEDical Literature Analysis and Retrieval System OnLINE

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