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  1. Article ; Online: Development of the aganglionic colon following surgical rescue in a cell therapy model of Hirschsprung disease in rat

    John B. Furness / Enie Lei / Billie Hunne / Cameron D. Adams / Alan J. Burns / Jill Wykosky / Therese E. Fazio Coles / Linda J. Fothergill / Juan C. Molero / Ruslan V. Pustovit / Lincon A. Stamp

    Disease Models & Mechanisms, Vol 16, Iss

    2023  Volume 6

    Keywords hirschsprung disease ; stem cell therapy ; colon ; intestinal bypass ; enteric neurons ; enteric nervous system ; Medicine ; R ; Pathology ; RB1-214
    Language English
    Publishing date 2023-06-01T00:00:00Z
    Publisher The Company of Biologists
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article: Cellular and sub-cellular localisation of oxyntomodulin-like immunoreactivity in enteroendocrine cells of human, mouse, pig and rat

    Fothergill, Linda J / Mitchell T. Ringuet / Efstathia Sioras / Billie Hunne / Therese E. Fazio Coles / Patricia R. Martins / John B. Furness

    Cell and tissue research. 2019 Feb., v. 375, no. 2

    2019  

    Abstract: We use a monoclonal antibody against the C-terminal of oxyntomodulin (OXM) to investigate enteroendocrine cells (EEC) in mouse, rat, human and pig. This antibody has cross-reactivity with the OXM precursor, glicentin (Gli) but does not recognise glucagon. ...

    Abstract We use a monoclonal antibody against the C-terminal of oxyntomodulin (OXM) to investigate enteroendocrine cells (EEC) in mouse, rat, human and pig. This antibody has cross-reactivity with the OXM precursor, glicentin (Gli) but does not recognise glucagon. The antibody stained EEC in the jejunum and colon of each species. We compared OXM/Gli immunoreactivity with that revealed by antibodies against structurally related peptides, GLP-1 and glucagon and against GIP and PYY that are predicted to be in some EEC that express OXM/Gli. We used super-resolution to locate immunoreactive vesicles. In the pancreas, OXM/Gli was in glucagon cells but was located in separate storage vesicles to glucagon. In jejunal EEC, OXM/Gli and GIP were in many of the same cells but often in separate vesicles, whereas PYY and OXM/Gli were commonly colocalised in the same storage vesicles of colonic EEC. When binding of anti-GLP-1 to the structurally related GIP was removed by absorption with GIP peptide, GLP-1 and OXM/Gli immunoreactivities were contained in the same population of EEC in the intestine. We conclude that anti-OXM/Gli is a more reliable marker than anti-GLP-1 for EEC expressing preproglucagon products. Storage vesicles that were immunoreactive for OXM/Gli were almost always immunoreactive for GLP-1. OXM concentrations, measured by ELISA, were highest in the distal ileum and colon. Lesser concentrations were found in more proximal parts of small intestine and pancreas. Very little was in the stomach. In EEC containing GIP and OXM/Gli, these hormones are packaged in different secretory vesicles. Separate packaging also occurred for OXM and glucagon, whereas OXM/Gli and PYY and OXM/Gli and GLP-1 were commonly contained together in secretory vesicles.
    Keywords absorption ; colon ; cross reaction ; enzyme-linked immunosorbent assay ; glucagon ; glucagon-like peptide 1 ; humans ; ileum ; jejunum ; mice ; monoclonal antibodies ; packaging ; pancreas ; rats ; secretory granules ; stomach ; swine
    Language English
    Dates of publication 2019-02
    Size p. 359-369.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    ZDB-ID 125067-x
    ISSN 1432-0878 ; 0302-766X
    ISSN (online) 1432-0878
    ISSN 0302-766X
    DOI 10.1007/s00441-018-2921-z
    Database NAL-Catalogue (AGRICOLA)

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