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Book ; Online: Kan landmanden påvirke kvaliteten af oksekødet

Therkildsen, Margrethe

2023

Abstract: Leverandørmøde for Spis Min Gris. Præsentation af ... ...

Abstract	Leverandørmøde for Spis Min Gris. Præsentation af GrOBEat
Keywords	Dairy cattle ; Food security ; food quality and human health ; Beef cattle ; Consumer issues
Language	Danish
Publishing date	2023-06-12
Publishing country	dk
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Book ; Online: Bæredygtig kødproduktion - Græsbaseret oksekød fra den økologiske mælkeproduktion

Christiansen, Iben Alber / Therkildsen, Margrethe

2023

Abstract: Description of the link betweem dairy production and meat ... ...

Abstract	Description of the link betweem dairy production and meat production
Keywords	Dairy cattle ; Food security ; food quality and human health ; Beef cattle ; Food systems
Language	Danish
Publishing date	2023-12-31
Publishing country	dk
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Satellite cells sourced from bull calves and dairy cows differs in proliferative and myogenic capacity – Implications for cultivated meat

Skrivergaard, Stig / Krøyer Rasmussen, Martin / Sahebekhtiari, Navid / Feveile Young, Jette / Therkildsen, Margrethe

Food Research International. 2023 Nov., v. 173 p.113217-

2023

Abstract: Cultivated meat produced with primary muscle satellite cells (SCs) will need a continuous supply of isolated cell material from relevant animal donors. Factors such as age, sex, and breed, along with the sustainability and availability of donor animals, ... ...

Abstract	Cultivated meat produced with primary muscle satellite cells (SCs) will need a continuous supply of isolated cell material from relevant animal donors. Factors such as age, sex, and breed, along with the sustainability and availability of donor animals, could determine the most appropriate donor type for an efficient production. In this study, we focus on the proliferation and differentiation of bovine SCs isolated from bull calf and dairy cow muscle samples. The proliferative performance of bull calf SCs was significantly better than SCs from dairy cows, however a dynamic differentiation assay revealed that the degree of fusion and formation of myotubes were similar between donor types. Furthermore, the proliferation of SCs from both donor types was enhanced using an in-house developed serum-free media compared to 10% FBS, which also delayed myogenic differentiation and increased final cell population density. Using gene chip transcriptomics, we identified several differentially expressed genes between the two donor types, which could help explain the observed cellular differences. This data also revealed a high biological variance between the three replicate animals within donor type, which seemed to be decreased when using our in-house serum-free media. With the use of the powerful imaging modalities of Cytation 5, we developed a novel high contrast brightfield-enabled label-free myotube quantification method along with a more efficient end-point fusion analysis using Phalloidin-staining. The results give new insights into the bovine SC biology and potential use of bull calves and dairy cows as relevant donor animals for cultivated beef cell sourcing. The newly developed differentiation assays will further enhance future research within the field of cultivated meat and SC biology.
Keywords	DNA microarrays ; beef ; calves ; dairy cows ; food research ; gene expression regulation ; muscle development ; muscles ; myotubes ; population density ; transcriptomics ; variance ; Cellular agriculture ; Donor type ; Bovine satellite cell sourcing ; Cell proliferation ; Myogenic differentiation ; Label-free quantification ; Image-based cytometry ; Transcriptomics.
Language	English
Dates of publication	2023-11
Publishing place	Elsevier Ltd
Document type	Article ; Online
ZDB-ID	1111695-x
ISSN	1873-7145 ; 0963-9969
ISSN (online)	1873-7145
ISSN	0963-9969
DOI	10.1016/j.foodres.2023.113217
Database	NAL-Catalogue (AGRICOLA)

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Article: Crossbreed and feed allowance affect final meat and eating quality of pigs from a free-range system

Therkildsen, Margrethe / Jensen, Søren Krogh / Kongsted, Anne-Grete

Livestock science. 2021 Aug., v. 250

2021

Abstract: This study compared the meat and eating quality from fattening pigs raised in a free-range system involving two cross-breeds: Duroc x (Landrace x Yorkshire) (DYL); and Tamworth x (Landrace x Yorkshire) (TYL) and two feeding strategies (semi-ad libitum ( ... ...

Abstract	This study compared the meat and eating quality from fattening pigs raised in a free-range system involving two cross-breeds: Duroc x (Landrace x Yorkshire) (DYL); and Tamworth x (Landrace x Yorkshire) (TYL) and two feeding strategies (semi-ad libitum (NORM) or restrictive feeding of concentrate (80-50% of semi-ad libitum; RES)). Sixteen female pigs from each cross-breed were strip-grazing diverse pasture with a grass-clover mixture and forage herbs (338 m²/pig) and root chicory (34 m²/pig) from the age of 88 days and an average live weight of 32.2 kg (SD=1.9), and were allocated to either the NORM or RES feeding strategy after a three-week training and adaptation period. The NORM pigs were slaughtered after 68 days and RES pigs were slaughtered after 90 days of experimental feeding, in order to allow them to obtain the same slaughter weight as NORM within each cross-breed. Meat and eating quality was determined on the M. longissimus lumborum (LL) and the M. biceps femoris (BF) removed twenty-two hours post mortem. The DYL were superior to the TYL crossbreed when it came to carcass weight (84.6 vs. 70.3 kg) and lean percentage (63.6 vs. 54.5%) no matter the feeding strategy. The RES feeding strategy caused smaller carcasses (8.7%) with higher lean percentage (+1.5%). The BF was tenderer from the DYL crossbreed, whereas the LL from the TYL crossbreed had less acid and metal but sweeter flavor. The restricted feeding strategy had a negative impact on the texture of specifically the loin both measured as Warner Bratzler shear force (+8 N, 4 days post mortem) and as tenderness evaluated by a sensory panel, whereas the flavor of the meat was unaffected. The restricted feeding caused a change towards more poly-unsaturated fatty acids and n3 fatty acids and a lower ratio of n6/n3 fatty acids in the meat, which is favorable from a human nutrition point of view.
Keywords	Duroc ; carcass weight ; chicory ; crossbreds ; feeding methods ; females ; flavor ; forage ; human nutrition ; landraces ; longissimus muscle ; meat ; pastures ; restricted feeding ; sensory evaluation ; slaughter weight ; texture
Language	English
Dates of publication	2021-08
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	2226176-X
ISSN	1878-0490 ; 1871-1413
ISSN (online)	1878-0490
ISSN	1871-1413
DOI	10.1016/j.livsci.2021.104584
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Article ; Online: Beef-on-dairy: Meat quality of veal and prediction of intramuscular fat using the Q-FOM™ Beef camera at the 5th-6th thoracic vertebra.

Drachmann, Fie F / Christensen, Mette / Esberg, Jakob / Lauridsen, Thomas / Fogh, Anders / Young, Jette F / Therkildsen, Margrethe

Meat science

2024 Volume 213, Page(s) 109503

Abstract: This study aims to describe the meat quality of young Holstein (HOL) beef-on-dairy heifers and bulls sired by Angus (ANG, n = 109), Charolais (CHA, n = 101) and Danish Blue (DBL, n = 127), and to investigate the performance of the handheld vision-based Q- ...

Abstract	This study aims to describe the meat quality of young Holstein (HOL) beef-on-dairy heifers and bulls sired by Angus (ANG, n = 109), Charolais (CHA, n = 101) and Danish Blue (DBL, n = 127), and to investigate the performance of the handheld vision-based Q-FOM™ Beef camera in predicting the intramuscular fat concentration (IMF%) in M. longissimus thoracis from carcasses quartered at the 5th-6th thoracic vertebra. The results showed significant differences between crossbreeds and sexes on carcass characteristics and meat quality. DBL × HOL had the highest EUROP conformation scores, whereas ANG × HOL had darker meat with higher IMF% (3.52%) compared to CHA × HOL (2.99%) and DBL × HOL (2.51%). Bulls had higher EUROP conformation scores than heifers, and heifers had higher IMF% (3.70%) than bulls (2.31%). These findings indicate the potential for producing high-quality meat from beef-on-dairy heifers and ANG bulls. The IMF% prediction model for Q-FOM performed well with R
MeSH term(s)	Animals ; Cattle ; Male ; Red Meat/analysis ; Female ; Adipose Tissue/chemistry ; Muscle, Skeletal/chemistry ; Thoracic Vertebrae ; Photography ; Color ; Breeding
Language	English
Publishing date	2024-03-26
Publishing country	England
Document type	Journal Article
ZDB-ID	753319-6
ISSN	1873-4138 ; 0309-1740
ISSN (online)	1873-4138
ISSN	0309-1740
DOI	10.1016/j.meatsci.2024.109503
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Article ; Online: Satellite cells sourced from bull calves and dairy cows differs in proliferative and myogenic capacity - Implications for cultivated meat.

Skrivergaard, Stig / Krøyer Rasmussen, Martin / Sahebekhtiari, Navid / Feveile Young, Jette / Therkildsen, Margrethe

Food research international (Ottawa, Ont.)

2023 Volume 173, Issue Pt 1, Page(s) 113217

Abstract	Cultivated meat produced with primary muscle satellite cells (SCs) will need a continuous supply of isolated cell material from relevant animal donors. Factors such as age, sex, and breed, along with the sustainability and availability of donor animals, could determine the most appropriate donor type for an efficient production. In this study, we focus on the proliferation and differentiation of bovine SCs isolated from bull calf and dairy cow muscle samples. The proliferative performance of bull calf SCs was significantly better than SCs from dairy cows, however a dynamic differentiation assay revealed that the degree of fusion and formation of myotubes were similar between donor types. Furthermore, the proliferation of SCs from both donor types was enhanced using an in-house developed serum-free media compared to 10% FBS, which also delayed myogenic differentiation and increased final cell population density. Using gene chip transcriptomics, we identified several differentially expressed genes between the two donor types, which could help explain the observed cellular differences. This data also revealed a high biological variance between the three replicate animals within donor type, which seemed to be decreased when using our in-house serum-free media. With the use of the powerful imaging modalities of Cytation 5, we developed a novel high contrast brightfield-enabled label-free myotube quantification method along with a more efficient end-point fusion analysis using Phalloidin-staining. The results give new insights into the bovine SC biology and potential use of bull calves and dairy cows as relevant donor animals for cultivated beef cell sourcing. The newly developed differentiation assays will further enhance future research within the field of cultivated meat and SC biology.
MeSH term(s)	Female ; Animals ; Cattle ; Male ; Satellite Cells, Skeletal Muscle/metabolism ; Culture Media, Serum-Free/metabolism ; Muscle Fibers, Skeletal ; Cell Differentiation ; Meat
Chemical Substances	Culture Media, Serum-Free
Language	English
Publishing date	2023-07-01
Publishing country	Canada
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1111695-x
ISSN	1873-7145 ; 0963-9969
ISSN (online)	1873-7145
ISSN	0963-9969
DOI	10.1016/j.foodres.2023.113217
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Article ; Online: Bovine Satellite Cells Isolated after 2 and 5 Days of Tissue Storage Maintain the Proliferative and Myogenic Capacity Needed for Cultured Meat Production.

Skrivergaard, Stig / Rasmussen, Martin Krøyer / Therkildsen, Margrethe / Young, Jette Feveile

International journal of molecular sciences

2021 Volume 22, Issue 16

Abstract: Cultured meat is an emerging alternative food technology which aims to deliver a more ethical, sustainable, and healthy muscle-tissue-derived food item compared to conventional meat. As start-up companies are rapidly forming and accelerating this ... ...

Abstract	Cultured meat is an emerging alternative food technology which aims to deliver a more ethical, sustainable, and healthy muscle-tissue-derived food item compared to conventional meat. As start-up companies are rapidly forming and accelerating this technology, many aspects of this multi-faceted science have still not been investigated in academia. In this study, we investigated if bovine satellite cells with the ability to proliferate and undergo myogenic differentiation could be isolated after extended tissue storage, for the purpose of increasing the practicality for cultured meat production. Proliferation of bovine satellite cells isolated on the day of arrival or after 2 and 5 days of tissue storage were analyzed by metabolic and DNA-based assays, while their myogenic characteristics were investigated using RT-qPCR and immunofluorescence. Extended tissue storage up to 5 days did not negatively affect proliferation nor the ability to undergo fusion and create myosin heavy chain-positive myotubes. The expression patterns of myogenic and muscle-specific genes were also not affected after tissue storage. In fact, the data indicated a positive trend in terms of myogenic potential after tissue storage, although it was non-significant. These results suggest that the timeframe of which viable myogenic satellite cells can be isolated and used for cultured meat production can be greatly extended by proper tissue storage.
Language	English
Publishing date	2021-08-04
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms22168376
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Article ; Online: A simple and robust serum-free media for the proliferation of muscle cells.

Skrivergaard, Stig / Young, Jette Feveile / Sahebekhtiari, Navid / Semper, Cameron / Venkatesan, Meenakshi / Savchenko, Alexei / Stogios, Peter J / Therkildsen, Margrethe / Rasmussen, Martin Krøyer

Food research international (Ottawa, Ont.)

2023 Volume 172, Page(s) 113194

Abstract: Cultivated meat production requires an efficient, robust and highly optimized serum-free cell culture media for the needed upscaling of muscle cell expansion. Existing formulations of serum-free media are complex, expensive and have not been optimized ... ...

Abstract	Cultivated meat production requires an efficient, robust and highly optimized serum-free cell culture media for the needed upscaling of muscle cell expansion. Existing formulations of serum-free media are complex, expensive and have not been optimized for muscle cells. Thus, we undertook this work to develop a simple and robust serum-free media for the proliferation of bovine satellite cells (SCs) through Design of Experiment (DOE) and Response Surface Methodology (RSM) using precise and high-throughput image-based cytometry. Proliferative attributes were investigated with transcriptomics and long-term performance was validated using multiple live assays. Here we formulated a media based on three highly optimized components; FGF2 (2 ng/mL), fetuin (600 µg/mL) and BSA (75 µg/mL) which together with an insulin-transferrin-selenium (1x) supplement, sustained the proliferation of bovine SCs, porcine SCs and murine C2C12 muscle cells. Remarkably, cells cultured in our media named Tri-basal 2.0+ performed better than cell cultured in 10% FBS, with respect to proliferation. Hence, the optimized Tri-basal 2.0+ enhanced serum-free cell attachment and long-term proliferation, providing an alternative solution to the use of FBS in the production of cultivated meat.
MeSH term(s)	Animals ; Cattle ; Mice ; Swine ; Culture Media, Serum-Free ; Muscle Cells ; Muscles ; Biological Assay ; Cell Proliferation
Chemical Substances	Culture Media, Serum-Free
Language	English
Publishing date	2023-06-29
Publishing country	Canada
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1111695-x
ISSN	1873-7145 ; 0963-9969
ISSN (online)	1873-7145
ISSN	0963-9969
DOI	10.1016/j.foodres.2023.113194
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Article ; Online: A simple and robust serum-free media for the proliferation of muscle cells

Skrivergaard, Stig / Young, Jette Feveile / Sahebekhtiari, Navid / Semper, Cameron / Venkatesan, Meenakshi / Savchenko, Alexei / Stogios, Peter J. / Therkildsen, Margrethe / Rasmussen, Martin Krøyer

Food Research International. 2023 Oct., v. 172 p.113194-

2023

Abstract	Cultivated meat production requires an efficient, robust and highly optimized serum-free cell culture media for the needed upscaling of muscle cell expansion. Existing formulations of serum-free media are complex, expensive and have not been optimized for muscle cells. Thus, we undertook this work to develop a simple and robust serum-free media for the proliferation of bovine satellite cells (SCs) through Design of Experiment (DOE) and Response Surface Methodology (RSM) using precise and high-throughput image-based cytometry. Proliferative attributes were investigated with transcriptomics and long-term performance was validated using multiple live assays. Here we formulated a media based on three highly optimized components; FGF2 (2 ng/mL), fetuin (600 µg/mL) and BSA (75 µg/mL) which together with an insulin-transferrin-selenium (1x) supplement, sustained the proliferation of bovine SCs, porcine SCs and murine C2C12 muscle cells. Remarkably, cells cultured in our media named Tri-basal 2.0+ performed better than cell cultured in 10% FBS, with respect to proliferation. Hence, the optimized Tri-basal 2.0+ enhanced serum-free cell attachment and long-term proliferation, providing an alternative solution to the use of FBS in the production of cultivated meat.
Keywords	cattle ; cell culture ; fetuins ; food research ; meat ; meat production ; mice ; muscles ; response surface methodology ; swine ; transcriptomics ; Bovine satellite cells ; Serum-free media optimization ; Cultivated meat ; Image-based cytometry
Language	English
Dates of publication	2023-10
Publishing place	Elsevier Ltd
Document type	Article ; Online
ZDB-ID	1111695-x
ISSN	1873-7145 ; 0963-9969
ISSN (online)	1873-7145
ISSN	0963-9969
DOI	10.1016/j.foodres.2023.113194
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Article: Beef Production Systems with Steers of Dairy and Dairy × Beef Breeds Based on Forage and Semi-Natural Pastures.

Hessle, Anna / Therkildsen, Margrethe / Arvidsson-Segerkvist, Katarina

Animals : an open access journal from MDPI

2019 Volume 9, Issue 12

Abstract: This study compared animal performance and carcass characteristics in steers born to a dairy breed dam and a dairy or beef breed sire allocated to one out of two production systems, both including grazing semi-natural pastures during summer. Spring-born ... ...

Abstract	This study compared animal performance and carcass characteristics in steers born to a dairy breed dam and a dairy or beef breed sire allocated to one out of two production systems, both including grazing semi-natural pastures during summer. Spring-born groups comprising 16 purebred dairy (D) steers and 16 dairy × Charolais crossbreeds (C) were allocated to moderately high indoor feed intensity with slaughter at 21 months of age (H), while two corresponding autumn-born groups of 16 D and 16 C animals were allocated to low indoor feed intensity with slaughter at 28 months of age (L). The animals were mainly fed grass-clover silage while housed. The H steers grazed semi-natural pastures for one summer, whereas the L steers grazed semi-natural pastures for two summers. From weaning to slaughter, liveweight gain was 0.94 and 0.77 kg day
Language	English
Publishing date	2019-12-02
Publishing country	Switzerland
Document type	Journal Article
ISSN	2076-2615
ISSN	2076-2615
DOI	10.3390/ani9121064
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