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Article: Polycyclic Aromatic Hydrocarbon (PAH) Contents of Four Species of Smoked Fish from Different Sites in Senegal.

Diop, El Hadji Moussa / Ndiaye, Bou / Sow, Alioune / Sambe, Falilou Mbacké / Sall, Mamadou / Thiam, Mamadou Saliou

International journal of analytical chemistry

2023 Volume 2023, Page(s) 2931615

Abstract: Polycyclic aromatic hydrocarbons (PAHs) are compounds resulting from any incomplete combustion process. These are pollutants that have proven toxicity due to their carcinogenic nature and can contaminate food during traditional smoking methods. Their ... ...

Abstract	Polycyclic aromatic hydrocarbons (PAHs) are compounds resulting from any incomplete combustion process. These are pollutants that have proven toxicity due to their carcinogenic nature and can contaminate food during traditional smoking methods. Their highly toxic effect on human health requires monitoring of their levels in food products and the development of appropriate analytical methods for their determination. Thus, this study was conducted to assess the level of PAHs contamination of four (4) species of smoked fish (
Language	English
Publishing date	2023-05-13
Publishing country	Egypt
Document type	Journal Article
ZDB-ID	2494714-3
ISSN	1687-8779 ; 1687-8760
ISSN (online)	1687-8779
ISSN	1687-8760
DOI	10.1155/2023/2931615
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Article: Investigation of the Potential of Heterophil/Lymphocyte Ratio as a Biomarker to Predict Colonization Resistance and Inflammatory Response to Salmonella enteritidis Infection in Chicken

Thiam, Mamadou / Barreto Sánchez, Astrid Lissette / Zhang, Jin / Wen, Jie / Zhao, Guiping / Wang, Qiao

Pathogens. 2022 Jan. 07, v. 11, no. 1

2022

Abstract: Salmonella causes significant economic loss to the poultry industry and represents a real threat to human health. The region of difference 21 (ROD21) pathogenicity island removal is a genetic mechanism by which Salmonellaenteritidis (SE) invades the ... ...

Abstract	Salmonella causes significant economic loss to the poultry industry and represents a real threat to human health. The region of difference 21 (ROD21) pathogenicity island removal is a genetic mechanism by which Salmonellaenteritidis (SE) invades the intestinal epithelium and induces systemic infection in mice. The heterophil/lymphocyte (H/L) ratio reflects the chicken’s robustness and immune system status. The H/L ratio is considered a disease resistance trait, and it could be used as a marker for selecting Salmonella resistance in live chickens. However, the association of the H/L ratio with Salmonella resistance and the inflammatory response remains to be elucidated. Moreover, the kinetics of ROD21 excision in the intestine and immune organs of chickens is unknown. Therefore, this study aimed to investigate the bacterial load, the ROD21 excision, the IL-1β, IL-8, and INF-γ blood serum concentration kinetics, and the association with the H/L ratio in chicken at 1, 3, 7, and 21 days post-SE infection. The results showed a significant correlation between the H/L ratio and the bacterial load in the ileum and caecum at 7 dpi. The ROD21 pathogenicity island absolute and relative excision in the caecum were positively correlated at 1 dpi but negatively correlated at 7 dpi with the H/L ratio. However, in the liver, we found the opposite tendency. The association of the H/L ratio with IL-1β, IL-8, and INF-γ blood serum concentrations showed that a low H/L ratio is correlated with increased IL-1β and INF-γ at 21 dpi. This study confirmed that the H/L ratio is associated with robustness and Salmonella-resistance in chicken. The methodology used in this study can separate individuals into susceptible and resistant and can help in the selection and breeding of Salmonella-resistant chickens.
Keywords	Salmonella Enteritidis ; biomarkers ; blood serum ; cecum ; chickens ; disease resistance ; excision ; financial economics ; heterophils ; human health ; ileum ; inflammation ; interleukin-8 ; intestinal mucosa ; liver ; microbial load ; pathogenicity islands ; poultry industry
Language	English
Dates of publication	2022-0107
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2695572-6
ISSN	2076-0817
ISSN	2076-0817
DOI	10.3390/pathogens11010072
Database	NAL-Catalogue (AGRICOLA)

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Article: Investigation of the Potential of Heterophil/Lymphocyte Ratio as a Biomarker to Predict Colonization Resistance and Inflammatory Response to

Thiam, Mamadou / Barreto Sánchez, Astrid Lissette / Zhang, Jin / Wen, Jie / Zhao, Guiping / Wang, Qiao

Pathogens (Basel, Switzerland)

2022 Volume 11, Issue 1

Abstract: ... ...

Abstract	Salmonella
Language	English
Publishing date	2022-01-07
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2695572-6
ISSN	2076-0817
ISSN	2076-0817
DOI	10.3390/pathogens11010072
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Article ; Online: Traitement de substitution des usagers dépendants des opiacés - L’expérience du Centre de prise en charge intégré des addictions de Dakar.

Gaye, Sader / Malissin, Isabelle / Gadji, Macoura / Thiam, Mamadou Habib / Mégarbane, Bruno

Medecine sciences : M/S

2022 Volume 38, Issue 10, Page(s) 832–837

Abstract: Methadone and buprenorphine are the two maintenance treatments in opiate addicts authorised in France since the end of the 1990's. More recently, some African countries such as Senegal have implemented a new health policy focused on reducing the risks by ...

Title translation	Substitution treatments for opiate-dependent users. An experience of the Integrated Addiction Treatment Center in Dakar.
Abstract	Methadone and buprenorphine are the two maintenance treatments in opiate addicts authorised in France since the end of the 1990's. More recently, some African countries such as Senegal have implemented a new health policy focused on reducing the risks by encouraging the use of methadone as maintenance treatment. The objectives of maintenance therapy are to reduce morbidity and mortality related to the consumption of heroin and other street opioids, to promote the integration of drug users into the healthcare system, and more generally, to improve their social integration. However, this strategy might have limitations in practice. Here, we report the experience of the Integrated Addiction Treatment Center in Dakar, Senegal, and discuss ethical considerations at both the individual and collective levels, which may improve care of opiate-dependent users in practice, especially in Africa.
MeSH term(s)	Analgesics, Opioid/therapeutic use ; Buprenorphine/therapeutic use ; Heroin/therapeutic use ; Heroin Dependence/drug therapy ; Heroin Dependence/rehabilitation ; Humans ; Methadone/therapeutic use ; Opiate Alkaloids ; Senegal/epidemiology
Chemical Substances	Analgesics, Opioid ; Opiate Alkaloids ; Buprenorphine (40D3SCR4GZ) ; Heroin (70D95007SX) ; Methadone (UC6VBE7V1Z)
Language	French
Publishing date	2022-10-11
Publishing country	France
Document type	Journal Article
ZDB-ID	632733-3
ISSN	1958-5381 ; 0767-0974
ISSN (online)	1958-5381
ISSN	0767-0974
DOI	10.1051/medsci/2022125
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Article ; Online: Gene Co-Expression Network Analysis Reveals the Hub Genes and Key Pathways Associated with Resistance to

Wang, Qiao / Thiam, Mamadou / Barreto Sánchez, Astrid Lissette / Wang, Zixuan / Zhang, Jin / Li, Qinghe / Wen, Jie / Zhao, Guiping

International journal of molecular sciences

2023 Volume 24, Issue 5

Abstract: ... ...

Abstract	Salmonella
MeSH term(s)	Animals ; Humans ; Cecum/metabolism ; Chickens ; Membrane Proteins/metabolism ; Microbiota ; Poultry Diseases/genetics ; Poultry Diseases/microbiology ; Salmonella enteritidis ; Salmonella Infections, Animal/genetics ; Salmonella Infections, Animal/microbiology ; Host-Pathogen Interactions/genetics
Chemical Substances	EMC6 protein, human ; Membrane Proteins
Language	English
Publishing date	2023-03-02
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms24054824
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Article ; Online: They call us

Alio, Amina P / Khoudia, Abdou / Thiam, Mamadou H / Talawa, Drusilla A / Bamfonga, Gradi / Al Ansar, Abdoulaye / Ndour, Cheikh T / Ndoye, Omar

Culture, health & sexuality

2022 Volume 24, Issue 9, Page(s) 1289–1301

Abstract: Men who have sex with men living with HIV in majority Muslim communities face discrimination based on multiple forms of stigma at socio-cultural and legislative levels. This study aimed to explore qualitatively the experiences of men who have sex with ... ...

Abstract	Men who have sex with men living with HIV in majority Muslim communities face discrimination based on multiple forms of stigma at socio-cultural and legislative levels. This study aimed to explore qualitatively the experiences of men who have sex with men living with HIV in Dakar, Senegal. In-depth individual interviews were conducted with 30 Senegalese men aged 18 to 55 years, who self-reported as same-sex practising, Muslim, and receiving HIV treatment at health centres in Dakar. Interview data were analysed using an ethnographic phenomenological approach to explore their life experiences. Primary themes included: the self-discovery process; the social, religious and health ramifications of being same-sex practising; and stigma. Within the theme of stigma issues described included shame, blame (of self and others), and violence resulting from being a man who has sex with other men and/or being HIV seropositive. Those with undetectable viral load reported how HIV related stigma and burden diminished as their health improved. Disclosure of being men who have sex with men and/or HIV status, whether voluntary or not, affected experiences of violence and/or isolation. Addressing stigma at healthcare institutions and improving access to HIV treatment can help mitigate the burden of stigma affecting such men. Interventions to address their physical and psychosocial wellbeing require the engagement of multiple stakeholders, including religious and political leaders.
MeSH term(s)	Female ; HIV Infections/psychology ; Homosexuality, Male/psychology ; Humans ; Islam ; Male ; Senegal ; Sexual and Gender Minorities ; Social Stigma
Language	English
Publishing date	2022-06-01
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2023577-X
ISSN	1464-5351 ; 1369-1058
ISSN (online)	1464-5351
ISSN	1369-1058
DOI	10.1080/13691058.2022.2080273
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Article: Les niveaux d’impact psychologique, d’anxiété, de dépression et de stress de la population générale du Sénégal pendant la pandémie de COVID-19.

Mansouri, Faouz / Lahlou, Laila / Camara, Momar / Seck, Sokhna / Hadji Makhtar Ba, El / Thiam, Mamadou Habib

Annales medico-psychologiques

2022

Abstract: Objectives: - Assessing of Impact of Event, depression, anxiety and stress levels in the Senegalese general population during the COVID19 pandemic and identifying associated factors.: Methods: - Socio-demographic data, psychological impact of the ... ...

Title translation	Assessing psychological, anxiety, depression and stress levels of Senegalese general population during COVID-19 pandemic.
Abstract	Objectives: - Assessing of Impact of Event, depression, anxiety and stress levels in the Senegalese general population during the COVID19 pandemic and identifying associated factors. Methods: - Socio-demographic data, psychological impact of the pandemic, and mental health status of participants were collected using an online questionnaire administered to the general population of Senegal between April 1 Results: - We enrolled 447 respondents with 19% reporting a moderate to severe psychological impact of the outbreak. Respectively 13.4%, 5.1% and 15.2% had moderate to extremely severe symptoms of depression, anxiety and stress. Statistically significant associations with higher odds of severe levels of psychological impact of the event, depression, anxiety, and stress were represented by five characteristics: Living in Dakar, no formal education, a personal medical chronic disease, a close family member with a chronic medical disease and poor to very poor self-reported health status. Conclusion: - This study determined the prevalence of post-traumatic stress disorder, depression, anxiety, and stress in the Senegalese general population during the COVID19 pandemic. Our study assessed the prevalence of post-traumatic stress disorder, depression, anxiety, and stress in the general population of Senegal during the COVID pandemic19. It confirms that the pandemic has truly an impact on the mental health of the Senegalese population. This impact is strongly influenced by geographic area and level of education.
Language	French
Publishing date	2022-09-06
Publishing country	France
Document type	English Abstract ; Journal Article
ZDB-ID	210836-7
ISSN	1769-6631 ; 0003-4487
ISSN (online)	1769-6631
ISSN	0003-4487
DOI	10.1016/j.amp.2022.09.005
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Article: Association of Heterophil/Lymphocyte Ratio with Intestinal Barrier Function and Immune Response to Salmonella enteritidis Infection in Chicken

Thiam, Mamadou / Barreto Sánchez, Astrid Lissette / Zhang, Jin / Zheng, Maiqing / Wen, Jie / Zhao, Guiping / Wang, Qiao

Animals. 2021 Dec. 08, v. 11, no. 12

2021

Abstract: The heterophil/lymphocyte (H/L) ratio has been extensively studied to select poultry that are resistant to environmental stressors. Chickens with a low H/L ratio are superior to the chickens with a high H/L ratio in survival, immune response, and ... ...

Abstract	The heterophil/lymphocyte (H/L) ratio has been extensively studied to select poultry that are resistant to environmental stressors. Chickens with a low H/L ratio are superior to the chickens with a high H/L ratio in survival, immune response, and resistance to Salmonella infection. However, this disease resistance ability is likely to be associated with enhanced intestinal immunity. Therefore, to expand our understanding of these underlying resistance mechanisms, it is crucial to investigate the correlation between the H/L ratio as a blood immune indicator in live chickens and the intestinal barrier function and immunity. Jinxing yellow chickens H/L line one-day-old were divided into non-infected (NI) and Salmonella enteritidis infected (SI) at 7-days old. After dividing the birds into NI and SI, blood samples were taken for H/L ratios determination, and subsequently, birds from the SI group were infected with Salmonella enteritidis (SE). We assessed the effects of SE infection on the (i) goblet cells number from the ileum and caecum gut-segments, (ii) ileal mucosa morphology, and (iii) immune gene mRNA expressions from the ileum and caecum of NI and SI chickens at 7 and 21 days-post-infection (dpi). We found that the H/L ratio was negatively correlated with most intestinal immune indices, particularly with the goblet cells number and with IL-1β, IL-8, and IFN-γ ileal expressions. In conclusion, these results suggest that the H/L ratio is associated with the intestinal barrier and immune response for SE clearance and that the chickens with a low H/L ratio displayed enhanced intestinal immunity. This study expands the current knowledge that is related to using the H/L ratio to select and breed resistant broiler chickens.
Keywords	Salmonella Enteritidis ; cecum ; disease resistance ; genes ; heterophils ; ileum ; immune response ; interleukin-8 ; salmonellosis
Language	English
Dates of publication	2021-1208
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2606558-7
ISSN	2076-2615
ISSN	2076-2615
DOI	10.3390/ani11123498
Database	NAL-Catalogue (AGRICOLA)

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Article: Liver Transcriptome Response to Heat Stress in Beijing You Chickens and Guang Ming Broilers

Barreto Sánchez, Astrid Lissette / Wang, Qiao / Thiam, Mamadou / Wang, Zixuan / Zhang, Jin / Zhang, Qi / Zhang, Na / Li, Qinghe / Wen, Jie / Zhao, Guiping

Genes. 2022 Feb. 25, v. 13, no. 3

2022

Abstract: Heat stress is one of the most prevalent issues in poultry production that reduces performance, robustness, and economic gains. Previous studies have demonstrated that native chickens are more tolerant of heat than commercial breeds. However, the ... ...

Abstract	Heat stress is one of the most prevalent issues in poultry production that reduces performance, robustness, and economic gains. Previous studies have demonstrated that native chickens are more tolerant of heat than commercial breeds. However, the underlying mechanisms of the heat tolerance observed in native chicken breeds remain unelucidated. Therefore, we performed a phenotypical, physiological, liver transcriptome comparative analysis and WGCNA in response to heat stress in one native (Beijing You, BY) and one commercial (Guang Ming, GM) chicken breed. The objective of this study was to evaluate the heat tolerance and identify the potential driver and hub genes related to heat stress in these two genetically distinct chicken breeds. In brief, 80 BY and 60 GM, 21 days old chickens were submitted to a heat stress experiment for 5 days (33 °C, 8 h/day). Each breed was divided into experimental groups of control (Ctl) and heat stress (HS). The results showed that BY chickens were less affected by heat stress and displayed reduced DEGs than GM chickens, 365 DEGs and 382 DEGs, respectively. The transcriptome analysis showed that BY chickens exhibited enriched pathways related to metabolism activity, meanwhile GM chickens’ pathways were related to inflammatory reactions. CPT1A and ANGPTL4 for BY chickens, and HSP90B1 and HSPA5 for GM chickens were identified as potential candidate genes associated with HS. The WGCNA revealed TLR7, AR, BAG3 genes as hub genes, which could play an important role in HS. The results generated in this study provide valuable resources for studying liver transcriptome in response to heat stress in native and commercial chicken lines.
Keywords	heat ; heat stress ; heat tolerance ; liver ; metabolism ; poultry production ; transcriptome ; transcriptomics ; China
Language	English
Dates of publication	2022-0225
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2527218-4
ISSN	2073-4425
ISSN	2073-4425
DOI	10.3390/genes13030416
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: A heterophil/lymphocyte-selected population reveals the phosphatase PTPRJ is associated with immune defense in chickens.

Wang, Jie / Zhang, Jin / Wang, Qiao / Zhang, Qi / Thiam, Mamadou / Zhu, Bo / Ying, Fan / Elsharkawy, Mohamed Shafey / Zheng, Maiqing / Wen, Jie / Li, Qinghe / Zhao, Guiping

Communications biology

2023 Volume 6, Issue 1, Page(s) 196

Abstract: Quantification of leukocyte profiles is among the simplest measures of animal immune function. However, the relationship between H/L ratio and innate immunity and the measure's utility as an index for heterophil function remains to be analyzed. Variants ... ...

Abstract	Quantification of leukocyte profiles is among the simplest measures of animal immune function. However, the relationship between H/L ratio and innate immunity and the measure's utility as an index for heterophil function remains to be analyzed. Variants associated with H/L ratio were fine-mapped based on the resequencing of 249 chickens of different generations and an F2 segregating population generated by crossing selection and control lines. H/L ratio in the selection line was associated with a selective sweep of mutations in protein tyrosine phosphatase, receptor type J (PTPRJ), which affects proliferation and differentiation of heterophils through its downstream regulatory genes. The SNP downstream of PTPRJ (rs736799474) have a universal effect on H/L, with CC homozygotes exhibiting improved heterophil function because of downregulated PTPRJ expression. In short, we systematically elucidated the genetic basis of the change in heterophil function resulting from H/L selection by identifying the regulatory gene (PTPRJ) and causative SNP.
MeSH term(s)	Animals ; Chickens/genetics ; Phosphoric Monoester Hydrolases ; Neutrophils ; Leukocytes ; Lymphocytes
Chemical Substances	Phosphoric Monoester Hydrolases (EC 3.1.3.2)
Language	English
Publishing date	2023-02-18
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2399-3642
ISSN (online)	2399-3642
DOI	10.1038/s42003-023-04559-x
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