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Article ; Online: Coding-complete genome sequences of two strains of canine pneumovirus derived from dogs with upper respiratory disease in the United States.

Thieulent, Côme J / Carossino, Mariano / Peak, Laura / Wolfson, Wendy / Li, Ganwu / Balasuriya, Udeni B R

Microbiology resource announcements

2024 Volume 13, Issue 2, Page(s) e0105723

Abstract: Canine pneumovirus was detected by RT-qPCR in 2022 from nasal swabs collected from two dogs with upper respiratory disease in a shelter in Louisiana, United States. The genomes from the designated strains CPnV USA/LA/2022/124423 and USA/LA/2022/123696 ... ...

Abstract	Canine pneumovirus was detected by RT-qPCR in 2022 from nasal swabs collected from two dogs with upper respiratory disease in a shelter in Louisiana, United States. The genomes from the designated strains CPnV USA/LA/2022/124423 and USA/LA/2022/123696 were sequenced and show the closest similarity to the pneumonia virus of mice J3666.
Language	English
Publishing date	2024-01-30
Publishing country	United States
Document type	Journal Article
ISSN	2576-098X
ISSN (online)	2576-098X
DOI	10.1128/mra.01057-23
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Development and validation of multiplex one-step qPCR/RT-qPCR assays for simultaneous detection of SARS-CoV-2 and pathogens associated with feline respiratory disease complex.

Thieulent, Côme J / Carossino, Mariano / Peak, Laura / Wolfson, Wendy / Balasuriya, Udeni B R

PloS one

2024 Volume 19, Issue 3, Page(s) e0297796

Abstract: Feline respiratory disease complex (FRDC) is caused by a wide range of viral and bacterial pathogens. Both Influenza A virus (IAV) and Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) also induce respiratory diseases in cats. Two one-step ... ...

Abstract	Feline respiratory disease complex (FRDC) is caused by a wide range of viral and bacterial pathogens. Both Influenza A virus (IAV) and Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) also induce respiratory diseases in cats. Two one-step multiplex qPCR/RT-qPCR assays were developed and validated: FRA_1 (Feline respiratory assay 1) for the detection of four viral targets and FRA_2 for the detection of three bacteria associated with FRDC. Both multiplex assays demonstrated high specificity, efficiency (93.51%-107.8%), linearity (> 0.998), analytical sensitivity (≤ 15 genome copies/μl), repeatability (coefficient of variation [CV] < 5%), and reproducibility (CV < 6%). Among the 63 clinical specimens collected from FRDC-suspected cats, 92.1% were positive for at least one pathogen and co-infection was detected in 57.1% of samples. Mycoplasma felis (61.9%) was the most found pathogen, followed by feline herpesvirus-1 (30.2%), Chlamydia felis (28.7%) and feline calicivirus (27.0%). SARS-CoV-2 was detected in two specimens. In summary, this new panel of qPCR/RT-qPCR assays constitutes a useful and reliable tool for the rapid detection of SARS-CoV-2 and viral and bacterial pathogens associated with FRDC in cats.
MeSH term(s)	Cats ; Animals ; SARS-CoV-2/genetics ; Reproducibility of Results ; COVID-19/diagnosis ; Bacteria/genetics ; Respiratory Tract Diseases ; Sensitivity and Specificity
Language	English
Publishing date	2024-03-22
Publishing country	United States
Document type	Journal Article
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0297796
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Modulation of Equid Herpesvirus-1 Replication Dynamics

Hassanien, Rabab T / Thieulent, Côme J / Carossino, Mariano / Li, Ganwu / Balasuriya, Udeni B R

Viruses

2024 Volume 16, Issue 3

Abstract: 1) Background: equid alphaherpesvirus-1 (EHV-1) is a highly contagious viral pathogen prevalent in most horse populations worldwide. Genome-editing technologies such as CRISPR/Cas9 have become powerful tools for precise RNA-guided genome modifications; ( ...

Abstract	(1) Background: equid alphaherpesvirus-1 (EHV-1) is a highly contagious viral pathogen prevalent in most horse populations worldwide. Genome-editing technologies such as CRISPR/Cas9 have become powerful tools for precise RNA-guided genome modifications; (2) Methods: we designed single guide RNAs (sgRNA) to target three essential (ORF30, ORF31, and ORF7) and one non-essential (ORF74) EHV-1 genes and determine their effect on viral replication dynamics
MeSH term(s)	Animals ; Horses ; Gene Editing/methods ; RNA, Guide, CRISPR-Cas Systems ; CRISPR-Cas Systems ; Herpesvirus 1, Equid/genetics ; Genome, Viral
Chemical Substances	RNA, Guide, CRISPR-Cas Systems
Language	English
Publishing date	2024-03-06
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v16030409
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Article ; Online: Development and Validation of a Panel of One-Step Four-Plex qPCR/RT-qPCR Assays for Simultaneous Detection of SARS-CoV-2 and Other Pathogens Associated with Canine Infectious Respiratory Disease Complex.

Thieulent, Côme J / Carossino, Mariano / Peak, Laura / Strother, Keith / Wolfson, Wendy / Balasuriya, Udeni B R

Viruses

2023 Volume 15, Issue 9

Abstract: Canine infectious respiratory disease complex (CIRDC) is the primary cause of respiratory disease in the canine population and is caused by a wide array of viruses and bacterial pathogens with coinfections being common. Since its recognition in late 2019, ...

Abstract	Canine infectious respiratory disease complex (CIRDC) is the primary cause of respiratory disease in the canine population and is caused by a wide array of viruses and bacterial pathogens with coinfections being common. Since its recognition in late 2019, Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) has been reported to cause respiratory disease in dogs. Therefore, the rapid detection and differentiation of SARS-CoV-2 from other common viral and bacterial agents is critical from a public health standpoint. Here, we developed and validated a panel of four one-step multiplex qPCR/RT-qPCR assays for the detection and identification of twelve pathogens associated with CIRDC (canine adenovirus-2, canine distemper virus, canine herpesvirus-1, canine influenza A virus, canine parainfluenza virus, canine pneumovirus, canine respiratory coronavirus, SARS-CoV-2,
MeSH term(s)	Dogs ; Animals ; SARS-CoV-2/genetics ; Coinfection/diagnosis ; Coinfection/veterinary ; Influenza A Virus, H1N1 Subtype ; Influenza A Virus, H3N2 Subtype ; Influenza A Virus, H3N8 Subtype ; COVID-19/diagnosis ; COVID-19/veterinary ; Respiratory Tract Infections/diagnosis ; Respiratory Tract Infections/veterinary ; Canidae
Language	English
Publishing date	2023-09-05
Publishing country	Switzerland
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v15091881
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Article ; Online: Multiplex One-Step RT-qPCR Assays for Simultaneous Detection of SARS-CoV-2 and Other Enteric Viruses of Dogs and Cats.

Thieulent, Côme J / Carossino, Mariano / Peak, Laura / Wolfson, Wendy / Balasuriya, Udeni B R

Viruses

2023 Volume 15, Issue 9

Abstract: The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) was transmitted from humans to dogs and cats (reverse zoonosis) during the COVID-19 pandemic. SARS-CoV-2 has been detected in fecal samples of infected dogs and cats, indicating potential ... ...

Abstract	The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) was transmitted from humans to dogs and cats (reverse zoonosis) during the COVID-19 pandemic. SARS-CoV-2 has been detected in fecal samples of infected dogs and cats, indicating potential fecal-oral transmission, environmental contamination, and zoonotic transmission (i.e., spillback). Additionally, gastrointestinal viral infections are prevalent in dogs and cats. In this study, we developed and validated a panel of multiplex one-step reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays for the simultaneous detection of SARS-CoV-2 and common canine enteric viruses: Canine Enteric Assay_1 (CEA_1) for the detection of canine adenovirus-1, canine enteric coronavirus, canine distemper virus, and canine parvovirus, and CEA_2 for the detection of rotavirus A (RVA), and SARS-CoV-2); or common feline enteric viruses (Feline Enteric Assay_1 (FEA_1) for the detection of feline enteric coronavirus, feline panleukopenia virus, RVA, and SARS-CoV-2). All assays demonstrated high analytical sensitivity, detecting as few as 5-35 genome copies/µL in multiplex format. The repeatability and reproducibility of the multiplex assays were excellent, with coefficient of variation <4%. Among the 58 clinical samples tested, 34.5% were positive for at least one of these viruses, and SARS-CoV-2 was detected in two samples collected from one dog and one cat, respectively. In conclusion, these newly developed one-step multiplex RT-qPCR assays allow for rapid diagnosis of enteric viral infections, including SARS-CoV-2, in dogs and cats.
MeSH term(s)	Dogs ; Cats ; Animals ; Humans ; SARS-CoV-2/genetics ; COVID-19/diagnosis ; COVID-19/veterinary ; Pandemics ; Cat Diseases/diagnosis ; Reproducibility of Results ; Dog Diseases/diagnosis ; Enterovirus ; Enterovirus Infections ; Rotavirus
Language	English
Publishing date	2023-09-07
Publishing country	Switzerland
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v15091890
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Quadruplex Real-Time TaqMan

Carossino, Mariano / Balasuriya, Udeni B R / Thieulent, Côme J / Barrandeguy, Maria E / Vissani, Maria Aldana / Parreño, Viviana

Viruses

2023 Volume 15, Issue 8

Abstract: Equine rotavirus A (ERVA) is the leading cause of diarrhea in foals, with G3P[12] and G14P[12] genotypes being the most prevalent. Recently, equine G3-like RVA was recognized as an emerging infection in children, and a group B equine rotavirus (ERVB) was ...

Abstract	Equine rotavirus A (ERVA) is the leading cause of diarrhea in foals, with G3P[12] and G14P[12] genotypes being the most prevalent. Recently, equine G3-like RVA was recognized as an emerging infection in children, and a group B equine rotavirus (ERVB) was identified as an emergent cause of foal diarrhea in the US. Thus, there is a need to adapt molecular diagnostic tools for improved detection and surveillance to identify emerging strains, understand their molecular epidemiology, and inform future vaccine development. We developed a quadruplex TaqMan
MeSH term(s)	Real-Time Polymerase Chain Reaction/methods ; Real-Time Polymerase Chain Reaction/veterinary ; Rotavirus/isolation & purification ; Animals ; Horses ; Horse Diseases/virology ; Rotavirus Infections/veterinary ; Feces/virology ; Sensitivity and Specificity
Language	English
Publishing date	2023-07-26
Publishing country	Switzerland
Document type	Evaluation Study ; Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v15081626
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Article ; Online: Mouse-Adapted SARS-CoV-2 MA10 Strain Displays Differential Pulmonary Tropism and Accelerated Viral Replication, Neurodissemination, and Pulmonary Host Responses in K18-hACE2 Mice.

Thieulent, Côme J / Dittmar, Wellesley / Balasuriya, Udeni B R / Crossland, Nicholas A / Wen, Xue / Richt, Juergen A / Carossino, Mariano

mSphere

2023 Volume 8, Issue 1, Page(s) e0055822

Abstract: Several models were developed to study the pathogenicity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as well as ... ...

Abstract	Several models were developed to study the pathogenicity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as well as the
MeSH term(s)	Mice ; Humans ; Animals ; SARS-CoV-2 ; COVID-19/pathology ; Angiotensin-Converting Enzyme 2/genetics ; Pandemics ; Lung/pathology ; Virus Replication ; Mice, Transgenic ; Tropism
Chemical Substances	Angiotensin-Converting Enzyme 2 (EC 3.4.17.23) ; K-18 conjugate
Language	English
Publishing date	2023-02-02
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
ISSN	2379-5042
ISSN (online)	2379-5042
DOI	10.1128/msphere.00558-22
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Development of a TaqMan

Thieulent, Come J / Carossino, Mariano / Balasuriya, Udeni B R / Graves, Kathryn / Bailey, Ernest / Eberth, John / Canisso, Igor F / Andrews, Frank M / Keowen, Michael L / Go, Yun Young

Frontiers in genetics

2022 Volume 13, Page(s) 871875

Abstract: Equine arteritis virus (EAV) is the causative agent of equine viral arteritis (EVA), a respiratory, systemic, and reproductive disease of equids. Following natural infection, up to 70% of the infected stallions can remain persistently infected over 1 ... ...

Abstract	Equine arteritis virus (EAV) is the causative agent of equine viral arteritis (EVA), a respiratory, systemic, and reproductive disease of equids. Following natural infection, up to 70% of the infected stallions can remain persistently infected over 1 year (long-term persistent infection [LTPI]) and shed EAV in their semen. Thus, the LTP-infected stallions play a pivotal role in maintaining and perpetuating EAV in the equine population. Previous studies identified equine C-X-C motif chemokine ligand 16 (CXCL16) as a critical host cell factor determining LTPI in the stallion's reproductive tract. Two alleles (
Language	English
Publishing date	2022-04-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2606823-0
ISSN	1664-8021
ISSN	1664-8021
DOI	10.3389/fgene.2022.871875
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Article: Oral Administration of Valganciclovir Reduces Clinical Signs, Virus Shedding and Cell-Associated Viremia in Ponies Experimentally Infected with the Equid Herpesvirus-1 C

Thieulent, Côme J / Sutton, Gabrielle / Toquet, Marie-Pierre / Fremaux, Samuel / Hue, Erika / Fortier, Christine / Pléau, Alexis / Deslis, Alain / Abrioux, Stéphane / Guitton, Edouard / Pronost, Stéphane / Paillot, Romain

Pathogens (Basel, Switzerland)

2022 Volume 11, Issue 5

Abstract: Equid alphaherpesvirus-1 (EHV-1) is one of the main pathogens in horses, responsible for respiratory diseases, ocular diseases, abortions, neonatal foal death and neurological complications such as equine herpesvirus myeloencephalopathy (EHM). Current ... ...

Abstract	Equid alphaherpesvirus-1 (EHV-1) is one of the main pathogens in horses, responsible for respiratory diseases, ocular diseases, abortions, neonatal foal death and neurological complications such as equine herpesvirus myeloencephalopathy (EHM). Current vaccines reduce the excretion and dissemination of the virus and, therefore, the extent of an epizooty. While their efficacy against EHV-1-induced abortion in pregnant mares and the decreased occurrence of an abortion storm in the field have been reported, their potential efficacy against the neurological form of disease remains undocumented. No antiviral treatment against EHV-1 is marketed and recommended to date. This study aimed to measure the protection induced by valganciclovir (VGCV), the prodrug of ganciclovir, in Welsh mountain ponies experimentally infected with an EHV-1 ORF30-C
Language	English
Publishing date	2022-05-04
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2695572-6
ISSN	2076-0817
ISSN	2076-0817
DOI	10.3390/pathogens11050539
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Article ; Online: Screening and evaluation of antiviral compounds against Equid alpha-herpesviruses using an impedance-based cellular assay.

Thieulent, Côme J / Hue, Erika S / Fortier, Christine I / Dallemagne, Patrick / Zientara, Stéphan / Munier-Lehmann, Hélène / Hans, Aymeric / Fortier, Guillaume D / Pitel, Pierre-Hugues / Vidalain, Pierre-Olivier / Pronost, Stéphane L

Virology

2018 Volume 526, Page(s) 105–116

Abstract: Equid alpha-herpesviruses (EHV) are responsible for different diseases in equine population. EHV-1 causes respiratory diseases, abortions and nervous disorders, EHV-4 causes respiratory diseases and sporadic abortion, while EHV-3 is responsible of equine ...

Abstract	Equid alpha-herpesviruses (EHV) are responsible for different diseases in equine population. EHV-1 causes respiratory diseases, abortions and nervous disorders, EHV-4 causes respiratory diseases and sporadic abortion, while EHV-3 is responsible of equine coital exanthema. In view of the lack of efficacy of vaccines against EHV-1 and EHV-4 and in the absence of vaccines against EHV-3, the use of antiviral treatment is of great interest. In this study, we documented the interest of the Real-Time Cell Analysis (RTCA) technology to monitor the cytopathic effects induced by these viruses on equine dermal cells, and established the efficacy of this method to evaluate the antiviral effect of aciclovir (ACV) and ganciclovir (GCV). In addition, the RTCA technology has also been found appropriate for the high-throughput screening of small molecules against EHV, allowing the identification of spironolactone as a novel antiviral against EHV.
MeSH term(s)	Animals ; Antiviral Agents/pharmacology ; Cell Line ; Cytopathogenic Effect, Viral/drug effects ; Electric Impedance ; Herpesviridae Infections/pathology ; Herpesviridae Infections/veterinary ; Herpesviridae Infections/virology ; Herpesvirus 1, Equid/classification ; Herpesvirus 1, Equid/drug effects ; Herpesvirus 3, Equid/drug effects ; Herpesvirus 4, Equid/drug effects ; High-Throughput Screening Assays/methods ; Horses ; Spironolactone/pharmacology
Chemical Substances	Antiviral Agents ; Spironolactone (27O7W4T232)
Language	English
Publishing date	2018-10-26
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	200425-2
ISSN	1096-0341 ; 0042-6822
ISSN (online)	1096-0341
ISSN	0042-6822
DOI	10.1016/j.virol.2018.10.013
Database	MEDical Literature Analysis and Retrieval System OnLINE

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