Article: Arginase 2 deletion leads to enhanced M1 macrophage activation and upregulated polyamine metabolism in response to Helicobacter pylori infection
Amino acids. 2016 Oct., v. 48, no. 10
2016
Abstract: We reported that arginase 2 (ARG2) deletion results in increased gastritis and decreased bacterial burden during Helicobacter pylori infection in mice. Our studies implicated a potential role for inducible nitric oxide (NO) synthase (NOS2), as Arg2 â»/â ...
| Abstract | We reported that arginase 2 (ARG2) deletion results in increased gastritis and decreased bacterial burden during Helicobacter pylori infection in mice. Our studies implicated a potential role for inducible nitric oxide (NO) synthase (NOS2), as Arg2 â»/â» mice exhibited increased NOS2 levels in gastric macrophages, and NO can kill H. pylori. We now bred Arg2 â»/â» to Nos2 â»/â» mice, and infected them with H. pylori. Compared to wild-type mice, both Arg2 â»/â» and Arg2 â»/â» ;Nos2 â»/â» mice exhibited increased gastritis and decreased colonization, the latter indicating that the effect of ARG2 deletion on bacterial burden was not mediated by NO. While Arg2 â»/â» mice demonstrated enhanced M1 macrophage activation, Nos2 â»/â» and Arg2 â»/â» ;Nos2 â»/â» mice did not demonstrate these changes, but exhibited increased CXCL1 and CXCL2 responses. There was an increased expression of the Th1/Th17 cytokines, interferon gamma and interleukin 17, in gastric tissues and splenic T-cells from Arg2 â»/â», but not Nos2 â»/â» or Arg2 â»/â» ;Nos2 â»/â» mice. Gastric tissues from infected Arg2 â»/â» mice demonstrated increased expression of arginase 1, ornithine decarboxylase, adenosylmethionine decarboxylase 1, spermidine/spermine N ¹-acetyltransferase 1, and spermine oxidase, along with increased spermine levels. These data indicate that ARG2 deletion results in compensatory upregulation of gastric polyamine synthesis and catabolism during H. pylori infection, which may contribute to increased gastric inflammation and associated decreased bacterial load. Overall, the finding of this study is that ARG2 contributes to the immune evasion of H. pylori by restricting M1 macrophage activation and polyamine metabolism. |
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| Keywords | adenosylmethionine decarboxylase ; arginase ; chemokine CXCL1 ; chemokine CXCL2 ; gastritis ; Helicobacter pylori ; immune evasion ; inducible nitric oxide synthase ; inflammation ; interferon-gamma ; interleukin-17 ; macrophage activation ; macrophages ; metabolism ; mice ; microbial load ; nitric oxide ; ornithine decarboxylase ; spermidine ; spermine ; T-lymphocytes | |||||
| Language | English | |||||
| Dates of publication | 2016-10 | |||||
| Size | p. 2375-2388. | |||||
| Publishing place | Springer Vienna | |||||
| Document type | Article | |||||
| ZDB-ID | 1121341-3 | |||||
| ISSN | 1438-2199 ; 0939-4451 | |||||
| ISSN (online) | 1438-2199 | |||||
| ISSN | 0939-4451 | |||||
| DOI | 10.1007/s00726-016-2231-2 | |||||
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| Database | NAL-Catalogue (AGRICOLA) |
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