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  1. Article: Isolation and characterization of methanesulfonic Acid-degrading bacteria from the marine environment.

    Thompson, A S / Owens, N / Murrell, J C

    Applied and environmental microbiology

    2006  Volume 61, Issue 6, Page(s) 2388–2393

    Abstract: Two methylotrophic bacterial strains, TR3 and PSCH4, capable of growth on methanesulfonic acid as the sole carbon source were isolated from the marine environment. Methanesulfonic acid metabolism in these strains was initiated by an inducible NADH- ... ...

    Abstract Two methylotrophic bacterial strains, TR3 and PSCH4, capable of growth on methanesulfonic acid as the sole carbon source were isolated from the marine environment. Methanesulfonic acid metabolism in these strains was initiated by an inducible NADH-dependent monooxygenase, which cleaved methanesulfonic acid into formaldehyde and sulfite. The presence of hydroxypyruvate reductase and the absence of ribulose monophosphate-dependent hexulose monophosphate synthase indicated the presence of the serine pathway for formaldehyde assimilation. Cell suspensions of bacteria grown on methanesulfonic acid completely oxidized methanesulfonic acid to carbon dioxide and sulfite with a methanesulfonic acid/oxygen stoichiometry of 1.0:2.0. Oxygen electrode-substrate studies indicated the dissimilation of formaldehyde to formate and carbon dioxide for energy generation. Carbon dioxide was not fixed by ribulose bisphosphate carboxylase. It was shown that methanol is not an intermediate in methanesulfonic acid metabolism, although these strains grew on methanol and other one-carbon compounds, as well as a variety of heterotrophic carbon sources. These two novel marine facultative methylotrophs have the ability to mineralize methanesulfonic acid and may play a role in the cycling of global organic sulfur.
    Language English
    Publishing date 2006-01-18
    Publishing country United States
    Document type Journal Article
    ZDB-ID 223011-2
    ISSN 1098-5336 ; 0099-2240
    ISSN (online) 1098-5336
    ISSN 0099-2240
    DOI 10.1128/aem.61.6.2388-2393.1995
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  2. Article: Determination of the structural role of the linking moieties in the DNA binding of adozelesin.

    Cameron, L / Thompson, A S

    Biochemistry

    2000  Volume 39, Issue 17, Page(s) 5004–5012

    Abstract: Adozelesin (formerly U73975, The Upjohn Co.) is a monofunctional DNA alkylating analogue of the antitumor antibiotic (+)-CC-1065. Adozelesin consists of a cyclopropa[c]pyrrolo[3,2-e]indol-4(5H)-one (CPI) alkylating subunit of (+)-CC-1065 and a indole and ...

    Abstract Adozelesin (formerly U73975, The Upjohn Co.) is a monofunctional DNA alkylating analogue of the antitumor antibiotic (+)-CC-1065. Adozelesin consists of a cyclopropa[c]pyrrolo[3,2-e]indol-4(5H)-one (CPI) alkylating subunit of (+)-CC-1065 and a indole and benzofurans subunit replacing the more complex pyrroloindole B and C subunits, respectively, of (+)-CC-1065. Previous studies have shown that adozelesin forms a reversible covalent DNA duplex adduct via a reaction between the N3 of adenine and the cyclopropyl of the cyclopropapyrroloindole (CPI) subunit. Gel electrophoresis studies have shown that adozelesin, like all the monofunctional (CPI)-based antitumor antibiotics, has a sequence preference for 5'-TTA-3' [the asterisk () indicates covalently modified base]. Molecular-modeling studies have shown that the bound adozelesin ligand spans a total of five base pairs including the modified adenine. These studies have also indicated that, owing to the orientation of the ligand within the base minor groove, there should be an overall preference for sequences rich in A.T base pairs, thus avoiding steric crowding around the exocyclic NH(2) of any guanines present. In this study, we have prepared and studied, by high-field NMR and restrained molecular mechanics (rMM) and dynamics (rMD), the duplex adduct formed between adozelesin and 5'-CGTAAGCGCTTACG-3'. Previous molecular-modeling studies suggested that this sequence should be less preferred, since the two GC base pairs should lead to extensive steric crowding within the adduct, and this hypothesis has, however, never been supported by DNA-footprinting data. (1)H NMR of the adozelesin duplex adduct has reveals that, although Watson-Crick base pairing is maintained throughout the DNA duplex, there is significant distortion around the central base pairs. This distortion is the result of strong hydrogen-bonding between the amide linker of the indole and benzofuran subunits, and the carbonyl of a central thymine base and second, weaker, hydrogen bond to the exocyclic NH(2) of the central guanine was also observed. (1)H NMR and rMD also indicate that, to accommodate this hydrogen-bond system, the bound adozelesin is not positioned centrally within the minor groove but pushed toward the modified DNA strand. Previous studies on the dimeric CPI analogue bizelesin have indicated the important role the ureylene linker plays in the DNA binding. This study indicates that a similar situation exists in the reaction of adozelesin with double-stranded DNA and provides a possible explanation into the unpredicted sequence selectivity of these ligands.
    MeSH term(s) Binding Sites ; Cyclohexanecarboxylic Acids/chemistry ; Cyclohexanecarboxylic Acids/metabolism ; Cyclohexenes ; DNA/chemistry ; DNA/metabolism ; Indoles ; Ligands ; Magnetic Resonance Spectroscopy ; Nucleic Acid Conformation
    Chemical Substances Cyclohexanecarboxylic Acids ; Cyclohexenes ; Indoles ; Ligands ; adozelesin (6N3M4XJR2V) ; DNA (9007-49-2)
    Language English
    Publishing date 2000-05-02
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1108-3
    ISSN 1520-4995 ; 0006-2960
    ISSN (online) 1520-4995
    ISSN 0006-2960
    DOI 10.1021/bi9926532
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    Zs.A 217: Show issues Location:
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  3. Article: Solution conformation of a bizelesin A-tract duplex adduct: DNA-DNA cross-linking of an A-tract straightens out bent DNA.

    Thompson, A S / Hurley, L H

    Journal of molecular biology

    1995  Volume 252, Issue 1, Page(s) 86–101

    Abstract: The DNA cross-linker bizelesin has been previously shown to form interhelical interstrand cross-links with adenine residues six base-pairs apart (including the modified adenine residues). Sequence specificity studies have shown that the ligand has a high ...

    Abstract The DNA cross-linker bizelesin has been previously shown to form interhelical interstrand cross-links with adenine residues six base-pairs apart (including the modified adenine residues). Sequence specificity studies have shown that the ligand has a high affinity for the intrinsically bent A-tract sequence [d(CGTTTTTACG):d(CGTAAAAACG)]. However, gel retardation studies have shown that the cross-linked duplex retains none of the characteristic A-tract bending observed within the unmodified duplex. Two-dimensional 1H-NMR experiments have not only confirmed the sites of cross-linking into the duplex, but have also shown the loss of inherent A-tract characteristics, including reduced crosspeak intensities between the H2s of the central adenine residues and the cross-strand H1' of the base one base removed to the 3' side. This observation suggests loss of propeller twisting within these central adenine residues and provides insight into the controversial origin of A-tract bending. This study is important because it validates the use of bizelesin as a probe for determining the importance of A-tract-induced bending in transcriptional and replicational elements.
    MeSH term(s) Adenine/chemistry ; Base Sequence ; Cross-Linking Reagents ; DNA Adducts/chemistry ; Hydrogen Bonding ; Indoles/chemistry ; Magnetic Resonance Spectroscopy ; Models, Molecular ; Molecular Sequence Data ; Nucleic Acid Conformation ; Solutions ; Urea/analogs & derivatives ; Urea/chemistry
    Chemical Substances Cross-Linking Reagents ; DNA Adducts ; Indoles ; Solutions ; Urea (8W8T17847W) ; Adenine (JAC85A2161) ; bizelesin (L0O9OBI87E)
    Language English
    Publishing date 1995-09-08
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 80229-3
    ISSN 1089-8638 ; 0022-2836
    ISSN (online) 1089-8638
    ISSN 0022-2836
    DOI 10.1006/jmbi.1994.0477
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  4. Article: Treatment outcome of sleep apnea.

    Watson, R K / Thompson, A S

    Connecticut medicine

    1992  Volume 56, Issue 3, Page(s) 125–129

    Abstract: Two hundred one patients diagnosed as having obstructive sleep apnea (OSA) were interviewed 12-24 months after their evaluation regarding those daytime symptoms associated with sleep apnea: sleepiness, fatigue, impaired memory, and snoring. Continuous ... ...

    Abstract Two hundred one patients diagnosed as having obstructive sleep apnea (OSA) were interviewed 12-24 months after their evaluation regarding those daytime symptoms associated with sleep apnea: sleepiness, fatigue, impaired memory, and snoring. Continuous positive airway pressure (CPAP) was the treatment most often used by severe sleep apnea patients, and this improved daytime alertness in 84% of the patients. Patients with moderate obstructive sleep apnea often had surgery which led to 85% reporting improved daytime alertness. Patients with mild obstructive sleep apnea usually were treated with weight loss or changing sleep position and also improved 64% and 66%. Patients who declined or failed treatment did not improve. Guidelines for the treatment of sleep apnea are suggested.
    MeSH term(s) Adolescent ; Adult ; Aged ; Child ; Child, Preschool ; Diet, Reducing/standards ; Evaluation Studies as Topic ; Female ; Humans ; Infant ; Male ; Middle Aged ; Positive-Pressure Respiration/standards ; Sleep Apnea Syndromes/diagnosis ; Sleep Apnea Syndromes/surgery ; Sleep Apnea Syndromes/therapy ; Surgical Procedures, Operative/standards ; Treatment Outcome
    Language English
    Publishing date 1992-03
    Publishing country United States
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 412600-2
    ISSN 0010-6178
    ISSN 0010-6178
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  5. Article: Field leg amputation by a paramedic.

    Kelly, J B / Thompson, A S / Gervin, A A

    Prehospital emergency care : official journal of the National Association of EMS Physicians and the National Association of State EMS Directors

    1999  Volume 3, Issue 1, Page(s) 77

    MeSH term(s) Accidents, Traffic ; Adult ; Allied Health Personnel ; Amputation ; Amputation, Traumatic/surgery ; Emergency Medical Services ; First Aid ; Humans ; Leg Injuries/surgery ; Male ; Multiple Trauma/surgery
    Language English
    Publishing date 1999-01
    Publishing country England
    Document type Case Reports ; Journal Article
    ZDB-ID 1461751-1
    ISSN 1545-0066 ; 1090-3127
    ISSN (online) 1545-0066
    ISSN 1090-3127
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  6. Article: Sixty years of what?

    Thompson, A S

    AARN news letter

    1976  Volume 32, Issue 7, Page(s) 1–9

    MeSH term(s) Alberta ; Education, Nursing ; History, 20th Century ; Legislation, Nursing ; Licensure, Nursing ; Nursing/standards ; Societies, Nursing/history
    Language English
    Publishing date 1976-07
    Publishing country Canada
    Document type Historical Article ; Journal Article
    ZDB-ID 603503-6
    ISSN 0001-0197
    ISSN 0001-0197
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  7. Article: Studies of Microthrix parvicella in situ and in laboratory culture: production and use of specific antibodies.

    Connery, N / Thompson, A S / Patrick, S / Larkin, M J

    Water science and technology : a journal of the International Association on Water Pollution Research

    2002  Volume 46, Issue 1-2, Page(s) 115–118

    Abstract: Physiological studies on M. parvicella have been conducted to determine the rate of growth of this organism in pure culture. The organism displayed a doubling time of 128 days despite its profuse abundance in a local Wastewater Treatment Plant (WWTW). An ...

    Abstract Physiological studies on M. parvicella have been conducted to determine the rate of growth of this organism in pure culture. The organism displayed a doubling time of 128 days despite its profuse abundance in a local Wastewater Treatment Plant (WWTW). An extensive survey has been ongoing since February 2000 into the extent of M. parvicella in the WWTW. A suite of monoclonal and polyclonal antibodies has been developed to detect and quantify M. parvicella.
    MeSH term(s) Actinobacteria/immunology ; Actinobacteria/isolation & purification ; Antibodies, Bacterial/analysis ; Antibodies, Monoclonal ; Population Dynamics ; Sewage/microbiology ; Waste Disposal, Fluid
    Chemical Substances Antibodies, Bacterial ; Antibodies, Monoclonal ; Sewage
    Language English
    Publishing date 2002
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 764273-8
    ISSN 1996-9732 ; 0273-1223
    ISSN (online) 1996-9732
    ISSN 0273-1223
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    Einzelsignatur: Show issues

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  8. Article ; Online: A genomic timescale for the origin of eukaryotes.

    Hedges, S B / Chen, H / Kumar, S / Wang, D Y / Thompson, A S / Watanabe, H

    BMC evolutionary biology

    2001  Volume 1, Page(s) 4

    Abstract: Background: Genomic sequence analyses have shown that horizontal gene transfer occurred during the origin of eukaryotes as a consequence of symbiosis. However, details of the timing and number of symbiotic events are unclear. A timescale for the early ... ...

    Abstract Background: Genomic sequence analyses have shown that horizontal gene transfer occurred during the origin of eukaryotes as a consequence of symbiosis. However, details of the timing and number of symbiotic events are unclear. A timescale for the early evolution of eukaryotes would help to better understand the relationship between these biological events and changes in Earth's environment, such as the rise in oxygen. We used refined methods of sequence alignment, site selection, and time estimation to address these questions with protein sequences from complete genomes of prokaryotes and eukaryotes.
    Results: Eukaryotes were found to evolve faster than prokaryotes, with those eukaryotes derived from eubacteria evolving faster than those derived from archaebacteria. We found an early time of divergence (approximately 4 billion years ago, Ga) for archaebacteria and the archaebacterial genes in eukaryotes. Our analyses support at least two horizontal gene transfer events in the origin of eukaryotes, at 2.7 Ga and 1.8 Ga. Time estimates for the origin of cyanobacteria (2.6 Ga) and the divergence of an early-branching eukaryote that lacks mitochondria (Giardia) (2.2 Ga) fall between those two events.
    Conclusions: We find support for two symbiotic events in the origin of eukaryotes: one premitochondrial and a later mitochondrial event. The appearance of cyanobacteria immediately prior to the earliest undisputed evidence for the presence of oxygen (2.4-2.2 Ga) suggests that the innovation of oxygenic photosynthesis had a relatively rapid impact on the environment as it set the stage for further evolution of the eukaryotic cell.
    MeSH term(s) Animals ; Archaea/genetics ; Eubacterium/genetics ; Eukaryotic Cells ; Evolution, Molecular ; Genetic Variation/genetics ; Genome ; Genome, Archaeal ; Genome, Bacterial ; Genome, Protozoan ; Giardia/genetics ; Models, Genetic ; Phylogeny
    Language English
    Publishing date 2001-09-12
    Publishing country England
    Document type Comparative Study ; Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
    ISSN 1471-2148
    ISSN (online) 1471-2148
    DOI 10.1186/1471-2148-1-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: 5-aminoisoquinolinone, a potent inhibitor of poly (adenosine 5'-diphosphate ribose) polymerase, reduces myocardial infarct size.

    Wayman, N / McDonald, M C / Thompson, A S / Threadgill, M D / Thiemermann, C

    European journal of pharmacology

    2001  Volume 430, Issue 1, Page(s) 93–100

    Abstract: This study investigates the effects of a novel, water-soluble inhibitor of the activity of poly (adenosine 5'-diphosphate ribose) polymerase, 5-aminoisoquinolinone [5-aminoisoquinolin-1(2H)-one], on (i) poly (adenosine 5'-diphosphate ribose) polymerase ... ...

    Abstract This study investigates the effects of a novel, water-soluble inhibitor of the activity of poly (adenosine 5'-diphosphate ribose) polymerase, 5-aminoisoquinolinone [5-aminoisoquinolin-1(2H)-one], on (i) poly (adenosine 5'-diphosphate ribose) polymerase activity in rat cardiac myoblasts and (ii) the infarct size caused by regional myocardial ischaemia and reperfusion in the rat. Exposure of H9c2 cells to hydrogen peroxide (H2O2, 1 mM) caused a significant increase in poly (adenosine 5'-diphosphate ribose) polymerase activity and an 80-90% reduction in mitochondrial respiration (cellular injury). Pretreatment of these cells with 5-aminoisoquinolinone (0.003-1 mM) caused a concentration-dependent inhibition of poly (adenosine 5'-diphosphate ribose) polymerase activity (IC50: approximately 4.5 microM, n=6-9) and cell injury (EC50: approximately 4.45 microM, n=9). In a rat model of myocardial infarction, left anterior descending coronary artery occlusion (25 min) and reperfusion (2 h) resulted in an infarct size of 50+/-3%. Administration (1 min before reperfusion) of 5-aminoisoquinolinone reduced myocardial infarct size in a dose-related fashion. Thus, 5-aminoisoquinolinone is a potent inhibitor of poly (adenosine 5'-diphosphate ribose) polymerase activity in cardiac myoblasts and reduces myocardial infarct size in vivo.
    MeSH term(s) Animals ; Cells, Cultured ; Enzyme Inhibitors/pharmacology ; Heart/drug effects ; Hydrogen Peroxide ; Isoquinolines/chemical synthesis ; Isoquinolines/pharmacology ; Isoquinolines/therapeutic use ; Myocardial Ischemia/drug therapy ; Myocardial Ischemia/pathology ; Myocardial Reperfusion ; Myocardium/metabolism ; Myocardium/pathology ; Nitroblue Tetrazolium ; Poly(ADP-ribose) Polymerase Inhibitors ; Rats ; Staining and Labeling
    Chemical Substances 5-aminoisoquinolinone ; Enzyme Inhibitors ; Isoquinolines ; Poly(ADP-ribose) Polymerase Inhibitors ; Nitroblue Tetrazolium (298-83-9) ; Hydrogen Peroxide (BBX060AN9V)
    Language English
    Publishing date 2001-10-26
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80121-5
    ISSN 1879-0712 ; 0014-2999
    ISSN (online) 1879-0712
    ISSN 0014-2999
    DOI 10.1016/s0014-2999(01)01359-0
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