LIVIVO - Search results -

Search results

Result 1 - 10 of total 220

Search options

Article ; Online: Adaptation of influenza viruses to human airway receptors.

Thompson, Andrew J / Paulson, James C

2020 Volume 296, Page(s) 100017

Abstract: Through annual epidemics and global pandemics, influenza A viruses (IAVs) remain a significant threat to human health as the leading cause of severe respiratory disease. Within the last century, four global pandemics have resulted from the introduction ... ...

Abstract	Through annual epidemics and global pandemics, influenza A viruses (IAVs) remain a significant threat to human health as the leading cause of severe respiratory disease. Within the last century, four global pandemics have resulted from the introduction of novel IAVs into humans, with components of each originating from avian viruses. IAVs infect many avian species wherein they maintain a diverse natural reservoir, posing a risk to humans through the occasional emergence of novel strains with enhanced zoonotic potential. One natural barrier for transmission of avian IAVs into humans is the specificity of the receptor-binding protein, hemagglutinin (HA), which recognizes sialic-acid-containing glycans on host cells. HAs from human IAVs exhibit "human-type" receptor specificity, binding exclusively to glycans on cells lining the human airway where terminal sialic acids are attached in the α2-6 configuration (NeuAcα2-6Gal). In contrast, HAs from avian viruses exhibit specificity for "avian-type" α2-3-linked (NeuAcα2-3Gal) receptors and thus require adaptive mutations to bind human-type receptors. Since all human IAV pandemics can be traced to avian origins, there remains ever-present concern over emerging IAVs with human-adaptive potential that might lead to the next pandemic. This concern has been brought into focus through emergence of SARS-CoV-2, aligning both scientific and public attention to the threat of novel respiratory viruses from animal sources. In this review, we summarize receptor-binding adaptations underlying the emergence of all prior IAV pandemics in humans, maintenance and evolution of human-type receptor specificity in subsequent seasonal IAVs, and potential for future human-type receptor adaptation in novel avian HAs.
MeSH term(s)	Adaptation, Physiological ; Animals ; Binding Sites ; Biological Coevolution ; Birds/virology ; Hemagglutinin Glycoproteins, Influenza Virus/chemistry ; Hemagglutinin Glycoproteins, Influenza Virus/genetics ; Hemagglutinin Glycoproteins, Influenza Virus/metabolism ; Humans ; Influenza A virus/chemistry ; Influenza A virus/genetics ; Influenza A virus/metabolism ; Influenza in Birds/epidemiology ; Influenza in Birds/transmission ; Influenza in Birds/virology ; Influenza, Human/epidemiology ; Influenza, Human/transmission ; Influenza, Human/virology ; Models, Molecular ; Pandemics ; Polysaccharides/chemistry ; Polysaccharides/metabolism ; Protein Binding ; Receptors, Virus/chemistry ; Receptors, Virus/genetics ; Receptors, Virus/metabolism ; Respiratory System/virology ; Sialic Acids/chemistry ; Sialic Acids/metabolism ; Species Specificity
Chemical Substances	Hemagglutinin Glycoproteins, Influenza Virus ; Polysaccharides ; Receptors, Virus ; Sialic Acids
Keywords	covid19
Language	English
Publishing date	2020-11-22
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Review
ZDB-ID	2997-x
ISSN	1083-351X ; 0021-9258
ISSN (online)	1083-351X
ISSN	0021-9258
DOI	10.1074/jbc.REV120.013309
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Uc I Zs.108: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Evidence for an effect of receptor density on ligand occupancy and agonist EC

Jarvis, Gavin E / Thompson, Andrew J

Scientific reports

2019 Volume 9, Issue 1, Page(s) 19111

Abstract: Drug-receptor interaction theory predicts that proportional receptor occupancy is a function of ligand concentration as defined by a ligand-receptor affinity constant, and is independent of receptor density. However, we previously observed that the ... ...

Abstract	Drug-receptor interaction theory predicts that proportional receptor occupancy is a function of ligand concentration as defined by a ligand-receptor affinity constant, and is independent of receptor density. However, we previously observed that the EC
MeSH term(s)	Animals ; DNA, Complementary/metabolism ; Dose-Response Relationship, Drug ; Humans ; Ions ; Ligands ; Membrane Potentials/drug effects ; Models, Theoretical ; Oocytes/drug effects ; Oocytes/metabolism ; Patch-Clamp Techniques ; Protein Multimerization ; RNA/metabolism ; Receptors, Serotonin, 5-HT3/chemistry ; Serotonin/chemistry ; Xenopus laevis
Chemical Substances	DNA, Complementary ; Ions ; Ligands ; Receptors, Serotonin, 5-HT3 ; Serotonin (333DO1RDJY) ; RNA (63231-63-0)
Language	English
Publishing date	2019-12-13
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-019-55361-x
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Elevated CO2 and high endogenous ABA level alleviate PEG-induced short-term osmotic stress in tomato plants

Li, Shenglan / Wang, Xizi / Liu, Xiaojuan / Thompson, Andrew J. / Liu, Fulai

Environmental and experimental botany. 2022 Feb., v. 194

2022

Abstract: Elevated CO₂ concentration (e[CO₂]) alleviates the impact of drought stress on plants where abscisic acid (ABA) is involved. To explore the mechanisms by which tomato plants respond to short-term osmotic stress, Solanum lycopersicum cv. Ailsa Craig (AC), ...

Abstract	Elevated CO₂ concentration (e[CO₂]) alleviates the impact of drought stress on plants where abscisic acid (ABA) is involved. To explore the mechanisms by which tomato plants respond to short-term osmotic stress, Solanum lycopersicum cv. Ailsa Craig (AC), a transgenic line overproducing ABA (sp5), and an ABA-deficient mutant (flacca) were hydroponically grown under ambient CO₂ (400 ppm) and e[CO₂] (800 ppm) and then exposed to 10% or 15% (w/v) polyethylene glycol (PEG) 6000 for 24 h before transferring to PEG-free nutrient solution for 24 h. Under non-stress condition, e[CO₂] decreased root hydraulic conductance (Kᵣₒₒₜ), which was overridden by high endogenous ABA in sp5 through increasing specific leaf area and root branching intensity. Basically, e[CO₂] improved stress resistance through enhanced water status. PEG stress decreased stomatal conductance and osmotic potential in AC but these effects were less pronounced in sp5, which exhibited a stronger osmotic adjustment (OA) and improved plant fitness. A greater flexibility of hydraulic system and a reduced sensitivity of Kᵣₒₒₜ to ABA might confer sp5 a great ability to recover from PEG stress. On the contrary, high stomatal density, size and pore aperture of flacca rendered plants suffering severe stress. Moreover, the premise that PEG stress could mimic soil water deficit was the sufficient achievement of OA. Our results indicate that e[CO₂] and high endogenous ABA level could improve osmotic stress resistance in tomato plants via osmotic and hydraulic adjustments.
Keywords	Solanum lycopersicum ; abscisic acid ; carbon dioxide ; carbon dioxide enrichment ; genetically modified organisms ; hydraulic conductivity ; hydroponics ; mutants ; nutrient solutions ; osmotic pressure ; osmotic stress ; polyethylene glycol ; soil water deficit ; specific leaf area ; stomatal conductance ; stress tolerance ; tomatoes ; water stress
Language	English
Dates of publication	2022-02
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	195968-2
ISSN	0098-8472
ISSN	0098-8472
DOI	10.1016/j.envexpbot.2021.104763
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 1950: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Recent developments in 5-HT3 receptor pharmacology.

Thompson, Andrew J

Trends in pharmacological sciences

2013 Volume 34, Issue 2, Page(s) 100–109

Abstract: Three decades ago the development of 5-hydroxytryptamine(3) (5-HT(3)) receptor antagonists had a major impact on the treatment of nausea and vomiting, and the cloning of the receptor a decade later enabled researchers to better understand its physiology. ...

Abstract	Three decades ago the development of 5-hydroxytryptamine(3) (5-HT(3)) receptor antagonists had a major impact on the treatment of nausea and vomiting, and the cloning of the receptor a decade later enabled researchers to better understand its physiology. In the last decade we have seen the publication of detailed molecular structures of closely related proteins, allowing us to reconcile the locations of binding-site residues with earlier pharmacological and biochemical studies. There are more than 500 5-HT(3) receptor ligands. The majority are competitive antagonists resulting from screens of structurally related analogues, but several non-competitive antagonists have also been described. Some ligands are noteworthy because they distinguish between receptor subtypes or have allosteric mechanisms. They will help us to further probe the physiological role of these receptors and could ultimately find applications in clinical and veterinary research. In this review, I consider recently identified ligands with an emphasis on their pharmacology and ligand-receptor interactions.
MeSH term(s)	Amino Acid Sequence ; Animals ; Binding Sites ; Binding, Competitive ; Humans ; Ligands ; Models, Molecular ; Molecular Sequence Data ; Receptors, Serotonin, 5-HT3/chemistry ; Receptors, Serotonin, 5-HT3/metabolism ; Serotonin 5-HT3 Receptor Agonists/chemistry ; Serotonin 5-HT3 Receptor Agonists/pharmacology ; Serotonin 5-HT3 Receptor Antagonists/chemistry ; Serotonin 5-HT3 Receptor Antagonists/pharmacology
Chemical Substances	Ligands ; Receptors, Serotonin, 5-HT3 ; Serotonin 5-HT3 Receptor Agonists ; Serotonin 5-HT3 Receptor Antagonists
Language	English
Publishing date	2013-02
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	282846-7
ISSN	1873-3735 ; 0165-6147
ISSN (online)	1873-3735
ISSN	0165-6147
DOI	10.1016/j.tips.2012.12.002
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1513: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 6: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Adaptation of Influenza Viruses to Human Airway Receptors

Thompson, Andrew J / Paulson, James C

J. biol. chem

Abstract	Through annual epidemics and global pandemics, influenza A viruses (IAVs) remain a significant threat to human health as the leading cause of severe respiratory disease. Within the last century, four global pandemics have resulted from the introduction of novel IAVs into humans, with components of each originating from avian viruses. IAVs infect many avian species wherein they maintain a diverse natural reservoir, posing a risk to humans through the occasional emergence of novel strains with enhanced zoonotic potential. One natural barrier for transmission of avian IAVs into humans is the specificity of the receptor-binding protein, hemagglutinin (HA), that recognizes sialic acid-containing glycans on host cells. HAs from human IAVs exhibit "human-type" receptor specificity, binding exclusively to glycans on cells lining the human airway where terminal sialic acids are attached in the α2-6 configuration (NeuAcα2-6Gal). In contrast, HAs from avian viruses exhibit specificity for "avian-type" α2-3-linked (NeuAcα2-3Gal) receptors, and thus require adaptive mutations to bind human-type receptors. Since all human IAV pandemics can be traced to avian origins, there remains ever-present concern over emerging IAVs with human-adaptive potential that might lead to the next pandemic. This concern has been brought into focus through emergence of SARS-CoV-2, aligning both scientific and public attention to the threat of novel respiratory viruses from animal sources. In this review we summarize receptor-binding adaptations underlying the emergence of all prior IAV pandemics in humans, maintenance and evolution of human-type receptor specificity in subsequent seasonal IAVs, and potential for future human-type receptor adaptation in novel avian HAs.
Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #910220
Database	COVID19

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article: A chromosome-level genome assembly of

Molitor, Corentin / Kurowski, Tomasz J / Fidalgo de Almeida, Pedro M / Kevei, Zoltan / Spindlow, Daniel J / Chacko Kaitholil, Steffimol R / Iheanyichi, Justice U / Prasanna, H C / Thompson, Andrew J / Mohareb, Fady R

Frontiers in plant science

2024 Volume 15, Page(s) 1342739

Abstract: Introduction: Solanum chilense: Methods: In this study we generated a high-quality, chromosome-level, : Results: The resulting sequences were arranged into 12 pseudomolecules using Hi-C sequencing. This resulted in a 901 Mbp assembly, with a ... ...

Abstract	Introduction: Solanum chilense Methods: In this study we generated a high-quality, chromosome-level, Results: The resulting sequences were arranged into 12 pseudomolecules using Hi-C sequencing. This resulted in a 901 Mbp assembly, with a completeness of 95%, as determined by Benchmarking with Universal Single-Copy Orthologs (BUSCO). Sequencing of RNA from multiple tissues resulting in ~219 Gbp of reads was used to annotate the genome assembly with an RNA-Seq guided gene prediction, and for a Discussion: Gene sequences related to drought and salt resistance were compared between
Language	English
Publishing date	2024-03-08
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2613694-6
ISSN	1664-462X
ISSN	1664-462X
DOI	10.3389/fpls.2024.1342739
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Genes involved in auxin biosynthesis, transport and signalling underlie the extreme adventitious root phenotype of the tomato aer mutant.

Kevei, Zoltan / Larriba, Eduardo / Romero-Bosquet, María Dolores / Nicolás-Albujer, Miriam / Kurowski, Tomasz J / Mohareb, Fady / Rickett, Daniel / Pérez-Pérez, José Manuel / Thompson, Andrew J

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

2024 Volume 137, Issue 4, Page(s) 76

Abstract: The use of tomato rootstocks has helped to alleviate the soaring abiotic stresses provoked by the adverse effects of climate change. Lateral and adventitious roots can improve topsoil exploration and nutrient uptake, shoot biomass and resulting overall ... ...

Abstract	The use of tomato rootstocks has helped to alleviate the soaring abiotic stresses provoked by the adverse effects of climate change. Lateral and adventitious roots can improve topsoil exploration and nutrient uptake, shoot biomass and resulting overall yield. It is essential to understand the genetic basis of root structure development and how lateral and adventitious roots are produced. Existing mutant lines with specific root phenotypes are an excellent resource to analyse and comprehend the molecular basis of root developmental traits. The tomato aerial roots (aer) mutant exhibits an extreme adventitious rooting phenotype on the primary stem. It is known that this phenotype is associated with restricted polar auxin transport from the juvenile to the more mature stem, but prior to this study, the genetic loci responsible for the aer phenotype were unknown. We used genomic approaches to define the polygenic nature of the aer phenotype and provide evidence that increased expression of specific auxin biosynthesis, transport and signalling genes in different loci causes the initiation of adventitious root primordia in tomato stems. Our results allow the selection of different levels of adventitious rooting using molecular markers, potentially contributing to rootstock breeding strategies in grafted vegetable crops, especially in tomato. In crops vegetatively propagated as cuttings, such as fruit trees and cane fruits, orthologous genes may be useful for the selection of cultivars more amenable to propagation.
MeSH term(s)	Indoleacetic Acids/metabolism ; Solanum lycopersicum/genetics ; Plant Breeding ; Signal Transduction ; Phenotype ; Plant Roots
Chemical Substances	Indoleacetic Acids
Language	English
Publishing date	2024-03-08
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2170-2
ISSN	1432-2242 ; 0040-5752
ISSN (online)	1432-2242
ISSN	0040-5752
DOI	10.1007/s00122-024-04570-8
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1692: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Identifying opportunities to improve management of water stress in banana production

Panigrahi, Niranjan / Thompson, Andrew J / Zubelzu, Sergio / Knox, Jerry W

Scientia horticulturae. 2021 Jan. 27, v. 276

2021

Abstract: Banana (Musa spp.) is one of the most valuable global agricultural commodities, with commercial plantations responsible for supplying nearly 15 % of total global banana production. These plantations are underpinned by major infrastructural investments ... ...

Abstract	Banana (Musa spp.) is one of the most valuable global agricultural commodities, with commercial plantations responsible for supplying nearly 15 % of total global banana production. These plantations are underpinned by major infrastructural investments and a high dependence on fertilizer, pesticide and irrigation inputs. In contrast, smallholders and subsistence farmers often cultivate bananas for local markets with minimal inputs. Water stress due to increasing rainfall variability and competition for water resources are emerging as major production constraints for both commercial and smallholder production. Water stress-induced yield losses of up to 65 % have been reported due to loss in bunch weight even in moderate to low rainfall areas. Thus, investments in more efficient irrigation systems and water-saving technologies are being widely promoted to increase water productivity through improved scheduling to reduce drainage and runoff losses. This paper synthesises scientific and industry evidence on crop growth and development including root and shoot development, plant water relations, and yield response to water. It also critiques the importance of irrigation scheduling for maximising irrigation efficiency. New evidence to support the synchronization of irrigation with crop water demand to reduce environmental impacts is provided. High variability in crop water demand (1200–2690 mm per year) was found to be linked to cultivar choice, crop development cycle, and fluctuating conditions in environmental and edaphic factors. The findings confirm that irrigation should be scheduled at moderate levels of soil water deficit sufficient to promote deep and extensive rooting while maintaining banana quality. Management practices are recommended to mitigate water stress without compromising yield under limited rainfall and irrigation conditions. The ratooning cycle of banana also affects rooting activity and crop coefficients (Kc) compared to other annual crops. These aspects need to be considered when improving irrigation and crop modelling for banana. The findings provide valuable new insights and evidence for scientists and practitioners involved in banana research and management.
Keywords	Musa ; bananas ; cultivars ; drainage ; fertilizers ; growth and development ; industry ; pesticides ; rain ; ratooning ; runoff ; soil water deficit ; water conservation ; water stress
Language	English
Dates of publication	2021-0127
Publishing place	Elsevier B.V.
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	185557-8
ISSN	0304-4238
ISSN	0304-4238
DOI	10.1016/j.scienta.2020.109735
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 2810: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: The muscarinic antagonists scopolamine and atropine are competitive antagonists at 5-HT3 receptors.

Lochner, Martin / Thompson, Andrew J

Neuropharmacology

2016 Volume 108, Page(s) 220–228

Abstract: Scopolamine is a high affinity muscarinic antagonist that is used for the prevention of post-operative nausea and vomiting. 5-HT3 receptor antagonists are used for the same purpose and are structurally related to scopolamine. To examine whether 5-HT3 ... ...

Abstract	Scopolamine is a high affinity muscarinic antagonist that is used for the prevention of post-operative nausea and vomiting. 5-HT3 receptor antagonists are used for the same purpose and are structurally related to scopolamine. To examine whether 5-HT3 receptors are affected by scopolamine we examined the effects of this drug on the electrophysiological and ligand binding properties of 5-HT3A receptors expressed in Xenopus oocytes and HEK293 cells, respectively. 5-HT3 receptor-responses were reversibly inhibited by scopolamine with an IC50 of 2.09 μM. Competitive antagonism was shown by Schild plot (pA2 = 5.02) and by competition with the 5-HT3 receptor antagonists [(3)H]granisetron (Ki = 6.76 μM) and G-FL (Ki = 4.90 μM). The related molecule, atropine, similarly inhibited 5-HT evoked responses in oocytes with an IC50 of 1.74 μM, and competed with G-FL with a Ki of 7.94 μM. The reverse experiment revealed that granisetron also competitively bound to muscarinic receptors (Ki = 6.5 μM). In behavioural studies scopolamine is used to block muscarinic receptors and induce a cognitive deficit, and centrally administered concentrations can exceed the IC50 values found here. It is therefore possible that 5-HT3 receptors are also inhibited. Studies that utilise higher concentrations of scopolamine should be mindful of these potential off-target effects.
MeSH term(s)	Animals ; Atropine/metabolism ; Atropine/pharmacology ; Binding, Competitive/drug effects ; Binding, Competitive/physiology ; Dose-Response Relationship, Drug ; Female ; Guinea Pigs ; HEK293 Cells ; Humans ; Male ; Muscarinic Antagonists/metabolism ; Muscarinic Antagonists/pharmacology ; Protein Structure, Secondary ; Protein Structure, Tertiary ; Receptors, Serotonin, 5-HT3/chemistry ; Receptors, Serotonin, 5-HT3/metabolism ; Scopolamine/metabolism ; Scopolamine/pharmacology ; Serotonin 5-HT3 Receptor Antagonists/metabolism ; Serotonin 5-HT3 Receptor Antagonists/pharmacology ; Xenopus laevis
Chemical Substances	Muscarinic Antagonists ; Receptors, Serotonin, 5-HT3 ; Serotonin 5-HT3 Receptor Antagonists ; Atropine (7C0697DR9I) ; Scopolamine (DL48G20X8X)
Language	English
Publishing date	2016-04-22
Publishing country	England
Document type	Journal Article
ZDB-ID	218272-5
ISSN	1873-7064 ; 0028-3908
ISSN (online)	1873-7064
ISSN	0028-3908
DOI	10.1016/j.neuropharm.2016.04.027
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ui I Zs.242: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Missense mutation of a class B heat shock factor is responsible for the tomato bushy root-2 phenotype.

Kevei, Zoltan / Ferreira, Silva Demetryus Silva / Casenave, Cristina Maria Perez / Kurowski, Tomasz / Mohareb, Fady / Rickett, Daniel / Stain, Chris / Thompson, Andrew J

Molecular horticulture

2022 Volume 2, Issue 1, Page(s) 4

Abstract: The bushy root-2 (brt-2) tomato mutant has twisting roots, and slower plant development. Here we used whole genome resequencing and genetic mapping to show that brt-2 is caused by a serine to cysteine (S75C) substitution in the DNA binding domain (DBD) ... ...

Abstract	The bushy root-2 (brt-2) tomato mutant has twisting roots, and slower plant development. Here we used whole genome resequencing and genetic mapping to show that brt-2 is caused by a serine to cysteine (S75C) substitution in the DNA binding domain (DBD) of a heat shock factor class B (HsfB) encoded by SolycHsfB4a. This gene is orthologous to the Arabidopsis SCHIZORIZA gene, also known as AtHsfB4. The brt-2 phenotype is very similar to Arabidopsis lines in which the function of AtHsfB4 is altered: a proliferation of lateral root cap and root meristematic tissues, and a tendency for lateral root cap cells to easily separate. The brt-2 S75C mutation is unusual because all other reported amino acid substitutions in the highly conserved DBD of eukaryotic heat shock factors are dominant negative mutations, but brt-2 is recessive. We further show through reciprocal grafting that brt-2 exerts its effects predominantly through the root genotype even through BRT-2 is expressed at similar levels in both root and shoot meristems. Since AtHsfB4 is induced by root knot nematodes (RKN), and loss-of-function mutants of this gene are resistant to RKNs, BRT-2 could be a target gene for RKN resistance, an important trait in tomato rootstock breeding.Gene & accession numbersSolycHsfB4a - Solyc04g078770.
Language	English
Publishing date	2022-02-08
Publishing country	England
Document type	Journal Article
ISSN	2730-9401
ISSN (online)	2730-9401
DOI	10.1186/s43897-022-00025-0
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED