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Article ; Online: ERCP remains a critical skill for HPB, Upper GI & General Surgeons.

Thomson, Benjamin N J

2023 Volume 93, Issue 7-8, Page(s) 1738–1739

MeSH term(s)	Humans ; Cholangiopancreatography, Endoscopic Retrograde ; Surgeons ; Digestive System Surgical Procedures
Language	English
Publishing date	2023-07-27
Publishing country	Australia
Document type	Editorial ; Comment
ZDB-ID	2050749-5
ISSN	1445-2197 ; 1445-1433 ; 0004-8682
ISSN (online)	1445-2197
ISSN	1445-1433 ; 0004-8682
DOI	10.1111/ans.18599
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Zs.A 180: Show issues

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Article ; Online: Preparation of a Single Cell Suspension from the Murine Iridocorneal Angle.

Thomson, Benjamin R / Quaggin, Susan E

Bio-protocol

2022 Volume 12, Issue 10

Abstract: Single cell RNA sequencing is a powerful tool that can be used to identify distinct cell types and transcriptomic differences within complex tissues. It has proven to be especially useful in tissues of the eye, where investigators have identified novel ... ...

Abstract	Single cell RNA sequencing is a powerful tool that can be used to identify distinct cell types and transcriptomic differences within complex tissues. It has proven to be especially useful in tissues of the eye, where investigators have identified novel cell types within the retina, anterior chamber, and iridocorneal angle and explored transcriptomic contribution to disease phenotypes in age-related macular degeneration. However, to obtain high quality results, the technique requires isolation of healthy single cells from the tissue of interest, seeking complete tissue digestion while minimizing stress and transcriptomic changes in the isolated cells prior to library preparation. Here, we present a protocol developed in our laboratory for isolation of live single cells from the murine iridocorneal angle, which includes Schlemm's canal and the trabecular meshwork, suitable for single cell RNA sequencing, flow cytometry, or other downstream analysis. Graphical abstract.
Language	English
Publishing date	2022-05-20
Publishing country	United States
Document type	Journal Article
ZDB-ID	2833269-6
ISSN	2331-8325 ; 2331-8325
ISSN (online)	2331-8325
ISSN	2331-8325
DOI	10.21769/BioProtoc.4426
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Article: Non-surgical interventions to control bleeding from arteriovenous fistulas and grafts inside and outside the hemodialysis unit: a scoping review.

Milosevic, Elizabeth / Forster, Adam / Moist, Louise / Rehman, Faisal / Thomson, Benjamin

Clinical kidney journal

2024 Volume 17, Issue 5, Page(s) sfae089

Abstract: Background: Prolonged bleeding from arteriovenous fistulas (AVF) and arteriovenous grafts (AVG) associates with worse outcomes; Within the hemodialysis unit these outcomes include anemia and quality of life disruptions, and outside the hemodialysis unit ...

Abstract	Background: Prolonged bleeding from arteriovenous fistulas (AVF) and arteriovenous grafts (AVG) associates with worse outcomes; Within the hemodialysis unit these outcomes include anemia and quality of life disruptions, and outside the hemodialysis unit includes fatal hemorrhage. However, various guidelines for AVF/AVG bleeding management inside and outside the hemodialysis unit lack consensus. Methods: A scoping review was conducted of four databases, from inception to 17 February 2024. The study population was hemodialysis patients experiencing bleeding from AVF or AVG. Studies that assessed non-operative management were included. Results: Sixteen studies met inclusion criteria. Most (14/16) addressed post-cannulation bleeding from AVF/AVG within the dialysis unit. Compared with standard dressings, hemostatic dressings (chitosan-, cellulose- or thrombin-based) decreased post-cannulation bleeding time at arterial and venous site 35.7%-84.0% ( Conclusions: In hemodialysis patents with bleeding from AVF/AVG, several alternative dressings or devices decrease post-cannulation bleeding time within the hemodialysis unit. Existing research has not established criteria on when it might be appropriate to use specialized dressings. There is very limited research on methods to control bleeding from AVF/AVG outside the hemodialysis unit. More data are required before evidence-based guidelines can be made. Recommendations for future research are provided.
Language	English
Publishing date	2024-03-27
Publishing country	England
Document type	Journal Article
ZDB-ID	2655800-2
ISSN	2048-8513 ; 2048-8505
ISSN (online)	2048-8513
ISSN	2048-8505
DOI	10.1093/ckj/sfae089
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Zs.A 6587: Show issues

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Article: Non-endothelial expression of Endomucin in the mouse and human choroid.

Brookins, Elysse / Serrano, Sophia E / Yacu, George S / Finer, Gal / Thomson, Benjamin R

bioRxiv : the preprint server for biology

2024

Abstract: Endomucin (EMCN) is a 261 AA transmembrane glycoprotein that is highly expressed by venous and capillary endothelial cells where it plays a role in VEGF-mediated angiogenesis and regulation of immune cell recruitment. However, it is better known as a ... ...

Abstract	Endomucin (EMCN) is a 261 AA transmembrane glycoprotein that is highly expressed by venous and capillary endothelial cells where it plays a role in VEGF-mediated angiogenesis and regulation of immune cell recruitment. However, it is better known as a histological marker, where it has become widespread due to the commercial availability of high-quality antibodies that work under a wide range of conditions and in many tissues. The specificity of EMCN staining has been well-validated in retinal vessels, but while it has been used extensively as a marker in other tissues of the eye, including the choroid, the pattern of expression has not been described in detail. Here, in addition to endothelial expression in the choriocapillaris and deeper vascular layers, we characterize a population of EMCN-positive perivascular cells in the mouse choroid that did not co-localize with cells expressing other endothelial markers such as PECAM1 or PODXL. To confirm that these cells represented a new population of EMCN-expressing stromal cells, we then performed single cell RNA sequencing in choroids from adult wild-type mice. Analysis of this new dataset confirmed that, in addition to endothelial cells,
Language	English
Publishing date	2024-03-12
Publishing country	United States
Document type	Preprint
DOI	10.1101/2024.03.08.584133
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Article ; Online: Should Canadian medical schools have trainees funded by Saudi Arabia?

Thomson, Benjamin K A

CMAJ : Canadian Medical Association journal = journal de l'Association medicale canadienne

2019 Volume 190, Issue 50, Page(s) E1485

MeSH term(s)	Canada ; Delivery of Health Care ; Saudi Arabia ; Schools, Medical
Language	English
Publishing date	2019-01-02
Publishing country	Canada
Document type	Letter ; Comment
ZDB-ID	215506-0
ISSN	1488-2329 ; 0008-4409 ; 0820-3946
ISSN (online)	1488-2329
ISSN	0008-4409 ; 0820-3946
DOI	10.1503/cmaj.70797
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Ua VI Zs.136: Show issues

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Article ; Online: How to do a single-stage perforator based nasolabial flap for reconstruction of early-stage tongue cancer.

Arahill-Whitham, Jacob Brian / Thomson, Benjamin James / Malayil, Vineetha / Surendra, Vishak

ANZ journal of surgery

2023 Volume 94, Issue 1-2, Page(s) 246–249

Abstract: Tongue cancers are one of the most common subsites of malignancy in the head and neck, of which the majority are squamous cell carcinoma (SCC). Reconstruction following ablative surgery is challenging because of the role of the tongue in articulation, ... ...

Abstract	Tongue cancers are one of the most common subsites of malignancy in the head and neck, of which the majority are squamous cell carcinoma (SCC). Reconstruction following ablative surgery is challenging because of the role of the tongue in articulation, deglutition and protection of the airway. Microvascular free flaps are the current gold standard of reconstruction but are not feasible in all patients. Local and regional flaps provide a less challenging, faster alternative and may be more appropriate in comorbid patients with high anaesthetic risk as well as those with previously irradiated neck and poor vasculature. Nasolabial flaps are not commonly used for tongue reconstruction, requiring a two-staged procedure to allow division of the pedicle. We submit a modification of nasolabial flap as an inferiorly based, islanded perforator flap. This allows for single-stage reconstruction of tongue and floor of the mouth defects following resection of early-stage tongue cancers.
MeSH term(s)	Humans ; Tongue Neoplasms/surgery ; Tongue/surgery ; Plastic Surgery Procedures ; Perforator Flap ; Carcinoma, Squamous Cell/surgery
Language	English
Publishing date	2023-11-20
Publishing country	Australia
Document type	Case Reports
ZDB-ID	2050749-5
ISSN	1445-2197 ; 1445-1433 ; 0004-8682
ISSN (online)	1445-2197
ISSN	1445-1433 ; 0004-8682
DOI	10.1111/ans.18776
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Zs.A 180: Show issues

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Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Correction to: Transition to practice curriculum for general internal medicine physicians: scoping review and canadian national survey.

Thomson, Benjamin / O'Halloran, Heather / Wu, Luke / Gauthier, Stephen / Taylor, David

BMC medical education

2023 Volume 23, Issue 1, Page(s) 334

Language	English
Publishing date	2023-05-14
Publishing country	England
Document type	Published Erratum
ZDB-ID	2044473-4
ISSN	1472-6920 ; 1472-6920
ISSN (online)	1472-6920
ISSN	1472-6920
DOI	10.1186/s12909-023-04317-x
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Article ; Online: Single-cell transcriptomics analysis of proliferative diabetic retinopathy fibrovascular membranes reveals AEBP1 as fibrogenesis modulator.

Corano Scheri, Katia / Lavine, Jeremy A / Tedeschi, Thomas / Thomson, Benjamin R / Fawzi, Amani A

JCI insight

2023 Volume 8, Issue 23

Abstract: The management of preretinal fibrovascular membranes, a devastating complication of advanced diabetic retinopathy (DR), remains challenging. We characterized the molecular profile of cell populations in these fibrovascular membranes to identify ... ...

Abstract	The management of preretinal fibrovascular membranes, a devastating complication of advanced diabetic retinopathy (DR), remains challenging. We characterized the molecular profile of cell populations in these fibrovascular membranes to identify potentially new therapeutic targets. Preretinal fibrovascular membranes were surgically removed from patients and submitted for single-cell RNA-Seq (scRNA-Seq). Differential gene expression was implemented to define the transcriptomics profile of these cells and revealed the presence of endothelial, inflammatory, and stromal cells. Endothelial cell reclustering identified subclusters characterized by noncanonical transcriptomics profile and active angiogenesis. Deeper investigation of the inflammatory cells showed a subcluster of macrophages expressing proangiogenic cytokines, presumably contributing to angiogenesis. The stromal cell cluster included a pericyte-myofibroblast transdifferentiating subcluster, indicating the involvement of pericytes in fibrogenesis. Differentially expressed gene analysis showed that Adipocyte Enhancer-binding Protein 1, AEBP1, was significantly upregulated in myofibroblast clusters, suggesting that this molecule may have a role in transformation. Cell culture experiments with human retinal pericytes (HRP) in high-glucose condition confirmed the molecular transformation of pericytes toward myofibroblastic lineage. AEBP1 siRNA transfection in HRP reduced the expression of profibrotic markers in high glucose. In conclusion, AEBP1 signaling modulates pericyte-myofibroblast transformation, suggesting that targeting AEBP1 could prevent scar tissue formation in advanced DR.
MeSH term(s)	Humans ; Diabetic Retinopathy/metabolism ; Retina/metabolism ; Pericytes/metabolism ; Glucose/metabolism ; Gene Expression Profiling ; Diabetes Mellitus/metabolism ; Carboxypeptidases/metabolism ; Repressor Proteins/genetics
Chemical Substances	Glucose (IY9XDZ35W2) ; AEBP1 protein, human ; Carboxypeptidases (EC 3.4.-) ; Repressor Proteins
Language	English
Publishing date	2023-11-02
Publishing country	United States
Document type	Journal Article
ISSN	2379-3708
ISSN (online)	2379-3708
DOI	10.1172/jci.insight.172062
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Article ; Online: Endothelial Tyrosine Kinase Tie1 Is Required for Normal Schlemm's Canal Development-Brief Report.

Du, Jing / Thomson, Benjamin R / Onay, Tuncer / Quaggin, Susan E

Arteriosclerosis, thrombosis, and vascular biology

2022 Volume 42, Issue 3, Page(s) 348–351

Abstract: Background: Schlemm's canal (SC) is a large vessel residing in the iridocorneal angle and is required to regulate aqueous humor outflow. Normal SC structure and function is indispensable for maintaining normal intraocular pressure, and elevated ... ...

Abstract	Background: Schlemm's canal (SC) is a large vessel residing in the iridocorneal angle and is required to regulate aqueous humor outflow. Normal SC structure and function is indispensable for maintaining normal intraocular pressure, and elevated intraocular pressure is a risk factor for development of glaucoma. Recent reports have identified a key role of the angiopoietin-Tie2 pathway for SC development and function; however, the role of the orphan receptor Tie1 has not been clarified. Methods: We used Tie1 knock out mice to study the function of Tie1 in SC development and function. Real-time quantitative polymerase chain reaction and Western blot analyses were used to verify Tie1 deletion. High-resolution microscopy of mouse SC whole mount and cross sections were used to study SC morphology. Measurement of intraocular pressure in live mice was used to study the impact of Tie1 on SC function. Results: Tie1 is highly expressed in both human and mouse SC. Tie1 knock out mice display hypomorphic SC and elevated intraocular pressure as a result of attenuated SC development. Conclusions: Tie1 is indispensable for SC development and function, supporting it as a novel target for future SC-targeted glaucoma therapies and a candidate gene for glaucoma in humans.
MeSH term(s)	Animals ; Anterior Chamber/enzymology ; Anterior Chamber/growth & development ; Aqueous Humor/physiology ; Endothelium, Corneal/enzymology ; Glaucoma/etiology ; Humans ; Intraocular Pressure/physiology ; Lymphatic Vessels/abnormalities ; Lymphatic Vessels/enzymology ; Lymphatic Vessels/physiology ; Mice ; Mice, Knockout ; Models, Animal ; Receptor, TIE-1/deficiency ; Receptor, TIE-1/genetics ; Receptor, TIE-1/metabolism
Chemical Substances	Receptor, TIE-1 (EC 2.7.10.1)
Language	English
Publishing date	2022-01-13
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	1221433-4
ISSN	1524-4636 ; 1079-5642
ISSN (online)	1524-4636
ISSN	1079-5642
DOI	10.1161/ATVBAHA.121.316692
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Article ; Online: Mycobacterium tuberculosis peritonitis in peritoneal dialysis patients: A scoping review.

Thomson, Benjamin K A / Vaughan, Stephen / Momciu, Bogdan

Nephrology (Carlton, Vic.)

2021 Volume 27, Issue 2, Page(s) 133–144

Abstract: Background: The clinical syndrome of Mycobacterium tuberculosis (M. tuberculosis) peritoneal dialysis (PD) peritonitis is poorly understood. Whether local tuberculosis (TB) patterns modify the clinical syndrome, and what factors associate with poor ... ...

Abstract	Background: The clinical syndrome of Mycobacterium tuberculosis (M. tuberculosis) peritoneal dialysis (PD) peritonitis is poorly understood. Whether local tuberculosis (TB) patterns modify the clinical syndrome, and what factors associate with poor outcomes is also unknown. Methods: A scoping review identified published cases of TB PD peritonitis. Cases from low- and high-TB burden areas were compared, and cases that did or did not suffer a poor clinical outcome were compared. Results: There were 216 cases identified. Demographics, presentation, diagnosis, treatment and outcomes were described. Significant delays in diagnosis were common (6.1 weeks) and were longer in patients from low-TB burden regions (7.3 vs. 3.7 weeks). In low-TB burden areas, slower diagnostic methods were more commonly used like PD fluid culture (64.3% vs. 32.7%), and treatment was less likely with quinolone antibiotics (6.9% vs. 34.1%). Higher national TB incidence and lower GDP per capita were found in cases that suffered PD catheter removal or death. Diagnostic delays were not longer in cases in which a patient suffered PD catheter removal or death. Cases that suffered death were older (51.9 vs. 45.1 years) and less likely female (37.8% vs. 55.7%). Removal of PD catheter was more common in cases in which a patient died (62.0% vs. 49.1%). Conclusions: Outcomes in TB PD peritonitis are best predicted by national TB incidence, patient age and sex. Several unique features are identified to alert clinicians to use more rapid diagnostic methods that might enhance outcomes in TB PD peritonitis.
MeSH term(s)	Humans ; Kidney Failure, Chronic/therapy ; Peritoneal Dialysis/adverse effects ; Peritonitis, Tuberculous/etiology
Language	English
Publishing date	2021-12-06
Publishing country	Australia
Document type	Journal Article ; Review
ZDB-ID	1303661-0
ISSN	1440-1797 ; 1320-5358
ISSN (online)	1440-1797
ISSN	1320-5358
DOI	10.1111/nep.13997
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