Article: Angiopoietin-like protein 4 is a chromatin-bound protein that enhances mammosphere formation
2021 Volume 20, Page(s) 8
Abstract: Introduction: Metastatic progression in triple-negative breast cancer (TNBC) patients occurs primarily because of nuclear reprogramming that includes chromatin remodeling and epigenetic modifications. The existing and most successful chemotherapies ... ...
| Abstract | Introduction: Metastatic progression in triple-negative breast cancer (TNBC) patients occurs primarily because of nuclear reprogramming that includes chromatin remodeling and epigenetic modifications. The existing and most successful chemotherapies available for metastatic TNBC target nuclear proteins or damage DNA. The objectives here are to investigate an undescribed role for the molecular biology of nuclear angiopoietin-like protein 4 (ANGPTL4) and to characterize the effect of ectopic overexpression of ANGPTL4 in the metastatic biology of TNBC. Materials and methods: Lentiviral-mediated transduction was used to overexpress ANGPTL4 in the TNBC cell line MD Anderson-metastatic breast cancer 231. The overexpression of ANGPTL4 was confirmed by western blot and ELISA. Subcellular fractionation, western blot, and immunofluorescence microscopy were used to characterize the intracellular localization of ANGPTL4. Mammosphere culture and the anchorage-independent growth assay analyzed the metastatic potential of the cell line. Xenograft assays assessed the effect of ANGPTL4 overexpression on TNBC metastases Results: The ANGPTL4 overexpressing cell line formed larger mammospheres and anchorage-independent colonies Conclusions: The ANGPTL4 overexpressing cell line showed |
|---|---|
| Language | English |
| Publishing date | 2021-06-17 |
| Publishing country | India |
| Document type | Journal Article |
| ZDB-ID | 2098237-9 |
| ISSN | 1477-3163 ; 1477-3163 ; 0974-6773 |
| ISSN (online) | 1477-3163 |
| ISSN | 1477-3163 ; 0974-6773 |
| DOI | 10.4103/jcar.JCar_20_20 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.