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Article ; Online: New animal model of chronic gout reproduces pathological features of the disease in humans

Yang Yang / Xi Chen / Jiwei Wang / Yihong Liu / Tao Tang / Richard Ward / Cheng Xiang / Peiqi Hao / Xianrun Sun / Guancong Luo / Su An / Tian-Rui Xu

RMD Open, Vol 9, Iss

2023 Volume 4

Abstract: Objectives Gout, as the most prevalent form of inflammatory arthritis, necessitates the use of animal models to investigate the molecular mechanisms involved in its development. Therefore, our objective was to develop a novel chronic mouse model of gout ... ...

Abstract	Objectives Gout, as the most prevalent form of inflammatory arthritis, necessitates the use of animal models to investigate the molecular mechanisms involved in its development. Therefore, our objective was to develop a novel chronic mouse model of gout that more closely mimics the progression of gout in humans.Methods A novel chronic mouse model of gout was established by a simple method, which does not require high technical proficiency, predominantly involves daily intraperitoneal injections of potassium oxonate for approximately 4 months, combined with a high fat-diet and injections of acetic acid into the hind paws to facilitate the formation of monosodium urate (MSU). Arthritis scores and paw oedema were assessed, behavioural tests were conducted, and histopathological and imaging evaluations of the arthritic paw joints were performed.Results After 4 months of induction, mice in the model group exhibited noticeable increases in arthritis severity, joint and cartilage damage, as well as bone erosion. Gomori’s methenamine silver stain revealed the presence of MSU crystal deposition or tophi in the paw joints or ankle joints of up to 37.9% of the model mice (11 out of 29 mice). Moreover, treatment with benzbromarone effectively prevented the further development of gout or tophi formation in model mice.Conclusions Our model more accurately replicates the pathological features of gouty arthritis compared with gout induced by MSU crystal injections. Therefore, it is particularly suitable for further investigations into the pathogenesis of gout and also serves as a valuable platform for screening potential antigout agents.
Keywords	Medicine ; R
Subject code	630
Language	English
Publishing date	2023-10-01T00:00:00Z
Publisher	BMJ Publishing Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: The big bang of genome editing technology

Ming SHAO / Tian-Rui XU / Ce-Shi CHEN

Zoological Research, Vol 37, Iss 4, Pp 191-

development and application of the CRISPR/Cas9 system in disease animal models

2016 Volume 204

Abstract: Targeted genome editing technology has been widely used in biomedical studies. The CRISPR-associated RNA-guided endonuclease Cas9 has become a versatile genome editing tool. The CRISPR/Cas9 system is useful for studying gene function through efficient ... ...

Abstract	Targeted genome editing technology has been widely used in biomedical studies. The CRISPR-associated RNA-guided endonuclease Cas9 has become a versatile genome editing tool. The CRISPR/Cas9 system is useful for studying gene function through efficient knock-out, knock-in or chromatin modification of the targeted gene loci in various cell types and organisms. It can be applied in a number of fields, such as genetic breeding, disease treatment and gene functional investigation. In this review, we introduce the most recent developments and applications, the challenges, and future directions of Cas9 in generating disease animal model. Derived from the CRISPR adaptive immune system of bacteria, the development trend of Cas9 will inevitably fuel the vital applications from basic research to biotechnology and bio-medicine.
Keywords	CRISPR/Cas9 ; Animal models ; Gene therapy ; Zoology ; QL1-991
Language	English
Publishing date	2016-07-01T00:00:00Z
Publisher	Science Press, PR China
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Soil water stress attenuate the growth and development but enhance the saponin synthesis of Panax notogesing during flowering stage

Liao, Peiran / Diqiu Liu / Tian-Rui Xu / Xiuming Cui / Ye Yang

Industrial crops and products. 2017 Dec. 01, v. 108

2017

Abstract: Water stress can affect the growth and secondary metabolites of plants. Thus, this study identified the effects of soil water conditions on the growth and development of Panax notoginseng, especially saponin synthesis. Panax notoginseng was grown in ... ...

Abstract	Water stress can affect the growth and secondary metabolites of plants. Thus, this study identified the effects of soil water conditions on the growth and development of Panax notoginseng, especially saponin synthesis. Panax notoginseng was grown in different soil water conditions (0.40, 0.55, 0.70, 0.85, 1.00 field capacity) for 30days, and related indicators were determined every 10days. Panax notoginseng could not survive under the content of 0.40 and 1.00 field capacity. Thus, their cultures were set at 0.55, 0.70, and 0.85 field capacity for experimental purposes. The results showed that agronomic traits, leaf water contents, chlorophyll contents, and photosynthetic efficiency in 0.70 field capacity were significantly higher than in 0.55 and 0.85 field capacity. In addition to flowers, contents of soluble total sugars and proteins increased significantly in all the parts under 0.55 and 0.85 field capacity. Compared with 0.70 field capacity treatment, total saponin content of aboveground organs increased significantly under 0.55 and 0.85 field capacity. And 18 key genes increased significantly but 2 key genes changed insignificantly in saponin synthesis pathway. These results suggested that 0.70 field capacity was the most suitable soil water condition for flowering stage Panax notoginseng and produced the best agronomic traits, while water stress (0.55 and 0.85 field capacity) produce more saponin contents by improving the expression of key genes in saponin synthesis pathway.
Keywords	agronomic traits ; chlorophyll ; field capacity ; flowers ; genes ; growth and development ; leaves ; Panax notoginseng ; photosynthesis ; proteins ; saponins ; secondary metabolites ; soil water ; sugars ; water stress
Language	English
Dates of publication	2017-1201
Size	p. 95-105.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1132158-1
ISSN	1872-633X ; 0926-6690
ISSN (online)	1872-633X
ISSN	0926-6690
DOI	10.1016/j.indcrop.2017.05.052
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Absence of mutation in miR-34a gene in a Chinese longevity population

Huan WU / Yong-Han HE / Tian-Rui XU / Qing-Peng KONG

Zoological Research, Vol 36, Iss 2, Pp 112-

2015 Volume 114

Keywords	Zoology ; QL1-991
Language	English
Publishing date	2015-03-01T00:00:00Z
Publisher	Science Press, PR China
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Impaired terpenoid backbone biosynthesis reduces saponin accumulation in Panax notoginseng under Cd stress

Liao, Peiran / Huilin Guan / Lanping Guo / Qi Chen / Tian-Rui Xu / Xiuming Cui / Ye Yang / Yue Shi / Ziwei Li

Functional plant biology. 2019, v. 46, no. 1

2019

Abstract: Panax notoginseng saponins (PNS) are major secondary metabolite of Panax notoginseng (Burk.) F.H. Chen. Previous studies identified that P. notoginseng planting soil usually with high content of Cd. However, the effects of Cd stress on the accumulation ... ...

Abstract	Panax notoginseng saponins (PNS) are major secondary metabolite of Panax notoginseng (Burk.) F.H. Chen. Previous studies identified that P. notoginseng planting soil usually with high content of Cd. However, the effects of Cd stress on the accumulation of PNS and the corresponding regulation mechanisms have yet to be reported. In the present study, the impact of Cd stress on the PNS accumulation of P. notoginseng was studied in pot culture experiments. The effect of Cd stress on antioxidant enzyme activity was studied using hydroponics. In addition, transcriptase sequencing analysis was used to study the effect of Cd stress on the expression of PNS metabolism transcripts in hydroponic experiments. Cd treatments significantly decreased the accumulation of PNS in the rhizome and main root. The sensitive concentration of antioxidant enzyme activity for both leaf and stem was 2.5μM, whereas the sensitive concentration for the root was 5.0μM. Transcriptome analysis showed that 5132 genes (2930 up- and 2202 downregulated) were regulated by 5.0μM Cd stress in the root of P. notoginseng. Among them, six upregulated differentially expressed genes (DEGs) were related to the methylerythritol 4-phosphate (MEP) pathway, whereas three of the downregulated DEGs were mevalonate kinase (MVK), phosphomevalonate kinase (PMK), and geranylgeranyl diphosphate synthase (type II, GGPS). Of the 15 transcripts selected for real-time quantitative-PCR, 13 were expressed in the same manner as identified using RNA-seq. In conclusion, Cd stress inhibited the accumulation of PNS in the root of P. notoginseng by reducing the expression of MVK, PMK, and GGPS in the terpenoid backbone biosynthesis pathway, and also caused by the removal of reactive oxygen species.
Keywords	antioxidants ; biosynthesis ; cadmium ; enzyme activity ; gene expression regulation ; genes ; geranylgeranyl diphosphate synthase ; hydroponics ; leaves ; Panax notoginseng ; planting ; pot culture ; quantitative polymerase chain reaction ; rhizomes ; saponins ; secondary metabolites ; sequence analysis ; soil ; terpenoids ; transcriptomics
Language	English
Size	p. 56-68.
Publishing place	CSIRO Publishing
Document type	Article
ZDB-ID	2071582-1
ISSN	1445-4408
ISSN	1445-4408
DOI	10.1071/FP18003
Database	NAL-Catalogue (AGRICOLA)

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Article: C-RAF function at the genome-wide transcriptome level: A systematic view

Huang, Ying / Xin-Yu Zhang / Su An / Yang Yang / Ying Liu / Qian Hao / Xiao-Xi Guo / Tian-Rui Xu

Gene. 2018 May 20, v. 656

2018

Abstract: C-RAF was the first member of the RAF kinase family to be discovered. Since its discovery, C-RAF has been found to regulate many fundamental cell processes, such as cell proliferation, cell death, and metabolism. However, the majority of these functions ... ...

Abstract	C-RAF was the first member of the RAF kinase family to be discovered. Since its discovery, C-RAF has been found to regulate many fundamental cell processes, such as cell proliferation, cell death, and metabolism. However, the majority of these functions are achieved through interactions with different proteins; the genes regulated by C-RAF in its active or inactive state remain unclear. In the work, we used RNA-seq analysis to study the global transcriptomes of C-RAF bearing or C-RAF knockout cells in quiescent or EGF activated states. We identified 3353 genes that are promoted or suppressed by C-RAF. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed that these genes are involved in drug addiction, cardiomyopathy, autoimmunity, and regulation of cell metabolism. Our results provide a panoramic view of C-RAF function, including known and novel functions, and have revealed potential targets for elucidating the role of C-RAF.
Keywords	autoimmunity ; cardiomyopathy ; cell death ; cell proliferation ; drug abuse ; gene ontology ; genes ; metabolism ; proteins ; sequence analysis ; transcriptome
Language	English
Dates of publication	2018-0520
Size	p. 53-59.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	391792-7
ISSN	1879-0038 ; 0378-1119
ISSN (online)	1879-0038
ISSN	0378-1119
DOI	10.1016/j.gene.2018.02.034
Database	NAL-Catalogue (AGRICOLA)

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Article: Converting ginsenosides from stems and leaves of Panax notoginseng by microwave processing and improving their anticoagulant and anticancer activities

Qu, Yuan / Cheng-Xiao Wang / Hui-Ying Liu / Jiang-Hua He / Tian-Rui Xu / Xiao-Xi Guo / Xiu-Ming Cui / Yan Luo / Ye Yang

RSC advances. 2018 Dec. 04, v. 8, no. 70

2018

Abstract: A microwave processing technology was applied to degrade saponins from the stems and leaves of Panax notoginseng. Six transformation products (1–6), named 20(S)-ginsenoside Rg3 (1), 20(R)-ginsenoside Rg3 (2), notoginsenoside SFt3 (3), ginsenoside Rk1 (4), ...

Abstract	A microwave processing technology was applied to degrade saponins from the stems and leaves of Panax notoginseng. Six transformation products (1–6), named 20(S)-ginsenoside Rg3 (1), 20(R)-ginsenoside Rg3 (2), notoginsenoside SFt3 (3), ginsenoside Rk1 (4), ginsenoside Rg5 (5), and 20(S)-ginsenoside Rh2 (6) were isolated and identified from a microwave processed extract of the stems and leaves of P. notoginseng (MEL). This transformation method was also applied for producing the minor ginsenosides in flowers, seeds and pedicels of P. notoginseng. The extract and compounds 1–6 in MEL were evaluated in vitro for anticancer and anticoagulant activities. The results showed that the MEL extract and transformation products had outstanding inhibitory activities against human cervical cancer Hela and lung cancer A549 cells. The strongest inhibitory effect was observed for 20(S)-Rh2 (6) with an IC50 value of 8.23 μM in Hela cells. Moreover, the results showed that the MEL significantly prolonged prothrombin time in a concentration-dependent manner. The anticoagulant effect of the MEL improved with the increased contents of Rk1, Rg5, and SFt3.
Keywords	anticoagulant activity ; anticoagulants ; antineoplastic activity ; flowers ; ginsenosides ; human cell lines ; humans ; inhibitory concentration 50 ; leaves ; lung neoplasms ; Panax notoginseng ; processing technology ; prothrombin ; seeds ; stems ; uterine cervical neoplasms
Language	English
Dates of publication	2018-1204
Size	p. 40471-40482.
Publishing place	The Royal Society of Chemistry
Document type	Article
ISSN	2046-2069
DOI	10.1039/c8ra08021f
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Protein Drugs Related to Allergic Reaction

Ji-Fu Wei / Tian-Rui Xu / Ming-Can Yu / Xing-Ding Zhou / Zuo-Tao Zhao / Yang Jin

BioMed Research International, Vol

2015 Volume 2015

Keywords	Medicine ; R
Language	English
Publishing date	2015-01-01T00:00:00Z
Publisher	Hindawi Publishing Corporation
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Visualization of the activation of the histamine H3 receptor (H3R) using novel fluorescence resonance energy transfer biosensors and their potential application to the study of H3R pharmacology

Liu, Ying / Hong Zeng / John D. Pediani / Li Ma / Lu‐Yao Chen / Mei Tang / Nan Wu / Qian Hao / Richard J. Ward / Su An / Tian‐Rui Xu / Xiao‐Xi Guo / Yang Yang

FEBS journal. 2018 June, v. 285, no. 12

2018

Abstract: Activation of the histamine‐3 receptor (H3R) is involved in memory processes and cognitive action, while blocking H3R activation can slow the progression of neurological disorders, such as Alzheimer's disease, schizophrenia and narcolepsy. To date, ... ...

Abstract	Activation of the histamine‐3 receptor (H3R) is involved in memory processes and cognitive action, while blocking H3R activation can slow the progression of neurological disorders, such as Alzheimer's disease, schizophrenia and narcolepsy. To date, however, no direct way to examine the activation of H3R has been utilized. Here, we describe a novel biosensor that can visualize the activation of H3R through an intramolecular fluorescence resonance energy transfer (FRET) signal. To achieve this, we constructed an intramolecular H3R FRET sensor with cyan fluorescent protein (CFP) attached at the C terminus and yellow fluorescent protein (YFP) inserted into the third intracellular loop. The sensor was found to internalize normally on agonist treatment. We measured FRET signals between the donor CFP and the acceptor YFP in living cells in real time, the results of which indicated that H3R agonist treatment (imetit or histamine) increases the FRET signal in a time‐ and concentration‐dependent manner with Kon and Koff values consistent with published data and which maybe correlated with decreasing cAMP levels and the promotion of ERK1/2 phosphorylation. The FRET signal was inhibited by H3R antagonists, and the introduction of mutations at F419A, F423A, L426A and L427A, once again, the promotion of ERK1/2 phosphorylation, was diminished. Thus, we have built a H3R biosensor which can visualize the activation of receptor through real‐time structure changes and which can obtain pharmacological kinetic data at the same time. The FRET signals may allow the sensor to become a useful tool for screening compounds and optimizing useful ligands.
Keywords	agonists ; Alzheimer disease ; antagonists ; biosensors ; cognition ; cyclic AMP ; energy transfer ; histamine ; ligands ; memory ; mutation ; narcolepsy ; pharmacology ; phosphorylation ; schizophrenia ; screening ; yellow fluorescent protein
Language	English
Dates of publication	2018-06
Size	p. 2319-2336.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	2173655-8
ISSN	1742-4658 ; 1742-464X
ISSN (online)	1742-4658
ISSN	1742-464X
DOI	10.1111/febs.14484
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: VHL deficiency augments anthracycline sensitivity of clear cell renal cell carcinomas by down-regulating ALDH2

Yao-Hui Gao / Zhao-Xia Wu / Li-Qi Xie / Cai-Xia Li / Yu-Qin Mao / Yan-Tao Duan / Bing Han / San-Feng Han / Yun Yu / Hao-Jie Lu / Peng-Yuan Yang / Tian-Rui Xu / Jing-Lin Xia / Guo-Qiang Chen / Li-Shun Wang

Nature Communications, Vol 8, Iss 1, Pp 1-

2017 Volume 14

Abstract: The VHL tumour suppressor gene is lost in approximately 70% of clear cell renal cell carcinoma (ccRCC). In this study, the authors demonstrate that VHL loss in these tumours augments anthracyclines chemotherapy by down-regulation of ALDH2. ...

Abstract	The VHL tumour suppressor gene is lost in approximately 70% of clear cell renal cell carcinoma (ccRCC). In this study, the authors demonstrate that VHL loss in these tumours augments anthracyclines chemotherapy by down-regulation of ALDH2.
Keywords	Science ; Q
Language	English
Publishing date	2017-06-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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