LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 92

Search options

  1. Book ; Online: Origin and spatiotemporal dynamics of the peroxisomal endomembrane system

    Rachubinski, Richard A. / Titorenko, Vladimir I.

    2015  

    Abstract: The peroxisome is an organelle with essential roles in lipid metabolism, maintenance of reactive oxygen species homeostasis, and anaplerotic replenishment of tricarboxylic acid cycle intermediates destined for mitochondria. Peroxisomes constitute a ... ...

    Abstract The peroxisome is an organelle with essential roles in lipid metabolism, maintenance of reactive oxygen species homeostasis, and anaplerotic replenishment of tricarboxylic acid cycle intermediates destined for mitochondria. Peroxisomes constitute a dynamic endomembrane system. The homeostatic state of this system is upheld via two pathways for assembling and maintaining the diverse peroxisomal compartments constituting it; the relative contribution of each pathway to preserving such system may vary in different organisms and under various physiological conditions. One pathway begins with the targeting of certain peroxisomal membrane proteins to an endoplasmic reticulum template and their exit from the template via pre-peroxisomal carriers; these carriers mature into metabolically active peroxisomes containing the entire complement of membrane and matrix proteins. Another pathway operates via growth and maturation of pre-existing peroxisomal precursors that do not originate from the endoplasmic reticulum; mature peroxisomes proliferate by undergoing fission. Recent studies have uncovered new roles for the peroxisomal endomembrane system in orchestrating important developmental decisions and defining organismal longevity. This Frontiers Special Topic Issue is focused on the advances in our understanding of how evolutionarily distant organisms coordinate the formation, maturation, proliferation, maintenance, inheritance and quality control of the peroxisomal endomembrane system and how peroxisomal endomembranes communicate with other cellular compartments to orchestrate complex biological processes and various developmental programs from inside the cell
    Keywords Physiology ; Science (General)
    Size 1 electronic resource (148 p.)
    Publisher Frontiers Media SA
    Document type Book ; Online
    Note English ; Open Access
    HBZ-ID HT020091033
    ISBN 9782889194643 ; 2889194647
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  2. Article ; Online: Aging and Age-related Disorders: From Molecular Mechanisms to Therapies.

    Titorenko, Vladimir I

    International journal of molecular sciences

    2019  Volume 20, Issue 13

    Abstract: Our understanding of the molecular mechanisms underlying cellular and organismal aging andaging-associated pathology has advanced greatly in recent years [ ... ]. ...

    Abstract Our understanding of the molecular mechanisms underlying cellular and organismal aging andaging-associated pathology has advanced greatly in recent years [...].
    MeSH term(s) Age Factors ; Aging ; Cellular Senescence ; Disease/etiology ; Epidemiology ; Humans
    Language English
    Publishing date 2019-07-03
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms20133280
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: Molecular and Cellular Mechanisms of Aging and Age-related Disorders.

    Titorenko, Vladimir I

    International journal of molecular sciences

    2018  Volume 19, Issue 7

    MeSH term(s) Aging/genetics ; Aging/metabolism ; Aging/physiology ; Animals ; Disease Models, Animal ; Epigenesis, Genetic ; Gene Expression ; Humans ; Longevity/physiology
    Language English
    Publishing date 2018-07-14
    Publishing country Switzerland
    Document type Editorial ; Introductory Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms19072049
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: Caloric restriction creates a metabolic pattern of chronological aging delay that in budding yeast differs from the metabolic design established by two other geroprotectors.

    Mohammad, Karamat / Titorenko, Vladimir I

    Oncotarget

    2021  Volume 12, Issue 7, Page(s) 608–625

    Abstract: Caloric restriction and ... ...

    Abstract Caloric restriction and the
    Language English
    Publishing date 2021-03-30
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2560162-3
    ISSN 1949-2553 ; 1949-2553
    ISSN (online) 1949-2553
    ISSN 1949-2553
    DOI 10.18632/oncotarget.27926
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article: Quantitative metabolomics of Saccharomyces Cerevisiae using liquid chromatography coupled with tandem mass spectrometry

    Mohammad, Karamat / Jiang, Heng / Titorenko, Vladimir I.

    Journal of visualized experiments. 2021 Jan. 05, , no. 167

    2021  

    Abstract: Metabolomics is a methodology used for the identification and quantification of many low-molecular-weight intermediates and products of metabolism within a cell, tissue, organ, biological fluid, or organism. Metabolomics traditionally focuses on water- ... ...

    Abstract Metabolomics is a methodology used for the identification and quantification of many low-molecular-weight intermediates and products of metabolism within a cell, tissue, organ, biological fluid, or organism. Metabolomics traditionally focuses on water-soluble metabolites. The water-soluble metabolome is the final product of a complex cellular network that integrates various genomic, epigenomic, transcriptomic, proteomic, and environmental factors. Hence, the metabolomic analysis directly assesses the outcome of the action for all these factors in a plethora of biological processes within various organisms. One of these organisms is the budding yeast Saccharomyces cerevisiae, a unicellular eukaryote with the fully sequenced genome. Because S. cerevisiae is amenable to comprehensive molecular analyses, it is used as a model for dissecting mechanisms underlying many biological processes within the eukaryotic cell. A versatile analytical method for the robust, sensitive, and accurate quantitative assessment of the water-soluble metabolome would provide the essential methodology for dissecting these mechanisms. Here we present a protocol for the optimized conditions of metabolic activity quenching in and water-soluble metabolite extraction from S. cerevisiae cells. The protocol also describes the use of liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) for the quantitative analysis of the extracted water-soluble metabolites. The LC-MS/MS method of non-targeted metabolomics described here is versatile and robust. It enables the identification and quantification of more than 370 water-soluble metabolites with diverse structural, physical, and chemical properties, including different structural isomers and stereoisomeric forms of these metabolites. These metabolites include various energy carrier molecules, nucleotides, amino acids, monosaccharides, intermediates of glycolysis, and tricarboxylic cycle intermediates. The LC-MS/MS method of non-targeted metabolomics is sensitive and allows the identification and quantitation of some water-soluble metabolites at concentrations as low as 0.05 pmol/µL. The method has been successfully used for assessing water-soluble metabolomes of wild-type and mutant yeast cells cultured under different conditions.
    Keywords Saccharomyces cerevisiae ; biochemical pathways ; energy ; epigenome ; eukaryotic cells ; genomics ; glycolysis ; liquid chromatography ; metabolites ; metabolome ; metabolomics ; models ; mutants ; nucleotides ; proteomics ; quantitative analysis ; tandem mass spectrometry ; transcriptomics ; water solubility ; yeasts
    Language English
    Dates of publication 2021-0105
    Size p. e62061.
    Publishing place Journal of Visualized Experiments
    Document type Article
    ZDB-ID 2259946-0
    ISSN 1940-087X
    ISSN 1940-087X
    DOI 10.3791/62061
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article ; Online: Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry.

    Mohammad, Karamat / Jiang, Heng / Titorenko, Vladimir I

    Journal of visualized experiments : JoVE

    2021  , Issue 167

    Abstract: Metabolomics is a methodology used for the identification and quantification of many low-molecular-weight intermediates and products of metabolism within a cell, tissue, organ, biological fluid, or organism. Metabolomics traditionally focuses on water- ... ...

    Abstract Metabolomics is a methodology used for the identification and quantification of many low-molecular-weight intermediates and products of metabolism within a cell, tissue, organ, biological fluid, or organism. Metabolomics traditionally focuses on water-soluble metabolites. The water-soluble metabolome is the final product of a complex cellular network that integrates various genomic, epigenomic, transcriptomic, proteomic, and environmental factors. Hence, the metabolomic analysis directly assesses the outcome of the action for all these factors in a plethora of biological processes within various organisms. One of these organisms is the budding yeast Saccharomyces cerevisiae, a unicellular eukaryote with the fully sequenced genome. Because S. cerevisiae is amenable to comprehensive molecular analyses, it is used as a model for dissecting mechanisms underlying many biological processes within the eukaryotic cell. A versatile analytical method for the robust, sensitive, and accurate quantitative assessment of the water-soluble metabolome would provide the essential methodology for dissecting these mechanisms. Here we present a protocol for the optimized conditions of metabolic activity quenching in and water-soluble metabolite extraction from S. cerevisiae cells. The protocol also describes the use of liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) for the quantitative analysis of the extracted water-soluble metabolites. The LC-MS/MS method of non-targeted metabolomics described here is versatile and robust. It enables the identification and quantification of more than 370 water-soluble metabolites with diverse structural, physical, and chemical properties, including different structural isomers and stereoisomeric forms of these metabolites. These metabolites include various energy carrier molecules, nucleotides, amino acids, monosaccharides, intermediates of glycolysis, and tricarboxylic cycle intermediates. The LC-MS/MS method of non-targeted metabolomics is sensitive and allows the identification and quantitation of some water-soluble metabolites at concentrations as low as 0.05 pmol/µL. The method has been successfully used for assessing water-soluble metabolomes of wild-type and mutant yeast cells cultured under different conditions.
    MeSH term(s) Chromatography, Liquid ; Fluorescence ; Glycolysis ; Metabolome ; Metabolomics ; Proteomics ; Saccharomyces cerevisiae/metabolism ; Solubility ; Tandem Mass Spectrometry/methods ; Water
    Chemical Substances Water (059QF0KO0R)
    Language English
    Publishing date 2021-01-05
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Video-Audio Media
    ZDB-ID 2259946-0
    ISSN 1940-087X ; 1940-087X
    ISSN (online) 1940-087X
    ISSN 1940-087X
    DOI 10.3791/62061
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  7. Article ; Online: Caloric restriction causes a distinct reorganization of the lipidome in quiescent and non-quiescent cells of budding yeast.

    Mohammad, Karamat / Orfanos, Emmanuel / Titorenko, Vladimir I

    Oncotarget

    2021  Volume 12, Issue 24, Page(s) 2351–2374

    Abstract: After budding yeast cells cultured in a nutrient-rich liquid medium with 0.2% glucose (under caloric restriction conditions) or 2% glucose (under non-caloric restriction conditions), ferment glucose to ethanol and then consume ethanol, they enter the ... ...

    Abstract After budding yeast cells cultured in a nutrient-rich liquid medium with 0.2% glucose (under caloric restriction conditions) or 2% glucose (under non-caloric restriction conditions), ferment glucose to ethanol and then consume ethanol, they enter the stationary phase. The process of their chronological aging begins. At that point, the yeast culture starts to accumulate quiescent and non-quiescent cells. Here, we purified the high- and low-density populations of quiescent and non-quiescent cells from the yeast cultures limited in calorie supply or not. We then employed mass spectrometry-based quantitative lipidomics to assess the aging-associated changes in high- and low-density cells' lipidomes. We found that caloric restriction, a geroprotective dietary intervention, alters the concentrations of many lipid classes through most of the chronological lifespan of the high- and low-density populations of quiescent and non-quiescent cells. Specifically, caloric restriction decreased triacylglycerol, increased free fatty acid, elevated phospholipid and amplified cardiolipin concentrations. Based on these findings, we propose a hypothetical model for a caloric restriction-dependent reorganization of lipid metabolism in budding yeast's quiescent and non-quiescent cells. We also discovered that caloric restriction creates lipidomic patterns of these cells that differ from those established by two other robust geroprotectors, namely the
    Language English
    Publishing date 2021-11-23
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2560162-3
    ISSN 1949-2553 ; 1949-2553
    ISSN (online) 1949-2553
    ISSN 1949-2553
    DOI 10.18632/oncotarget.28133
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  8. Article ; Online: Yeast chronological aging is linked to cell cycle regulation.

    Mohammad, Karamat / Titorenko, Vladimir I

    Cell cycle (Georgetown, Tex.)

    2018  Volume 17, Issue 9, Page(s) 1035–1036

    MeSH term(s) Caloric Restriction ; Cell Self Renewal/physiology ; Cell Survival/physiology ; Cellular Senescence/physiology ; G1 Phase Cell Cycle Checkpoints/physiology ; Saccharomyces cerevisiae/metabolism
    Language English
    Publishing date 2018-07-05
    Publishing country United States
    Document type Editorial ; Research Support, Non-U.S. Gov't
    ZDB-ID 2146183-1
    ISSN 1551-4005 ; 1538-4101 ; 1554-8627
    ISSN (online) 1551-4005
    ISSN 1538-4101 ; 1554-8627
    DOI 10.1080/15384101.2018.1478670
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 5881: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  9. Article ; Online: A laboratory test of evolutionary aging theories.

    Iouk, Tatiana / Titorenko, Vladimir I

    Aging

    2017  Volume 9, Issue 3, Page(s) 600–601

    MeSH term(s) Biological Evolution ; Humans ; Leukemia, Lymphocytic, Chronic, B-Cell ; Longevity ; PTEN Phosphohydrolase ; Tumor Suppressor Protein p53 ; Ubiquitin-Specific Peptidase 7
    Chemical Substances TP53 protein, human ; Tumor Suppressor Protein p53 ; PTEN Phosphohydrolase (EC 3.1.3.67) ; PTEN protein, human (EC 3.1.3.67) ; USP7 protein, human (EC 3.4.19.12) ; Ubiquitin-Specific Peptidase 7 (EC 3.4.19.12)
    Language English
    Publishing date 2017-04-26
    Publishing country United States
    Document type Editorial ; Comment
    ISSN 1945-4589
    ISSN (online) 1945-4589
    DOI 10.18632/aging.101215
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  10. Article ; Online: Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry.

    Mohammad, Karamat / Jiang, Heng / Hossain, Md Israil / Titorenko, Vladimir I

    Journal of visualized experiments : JoVE

    2020  , Issue 157

    Abstract: Lipids are structurally diverse amphipathic molecules that are insoluble in water. Lipids are essential contributors to the organization and function of biological membranes, energy storage and production, cellular signaling, vesicular transport of ... ...

    Abstract Lipids are structurally diverse amphipathic molecules that are insoluble in water. Lipids are essential contributors to the organization and function of biological membranes, energy storage and production, cellular signaling, vesicular transport of proteins, organelle biogenesis, and regulated cell death. Because the budding yeast Saccharomyces cerevisiae is a unicellular eukaryote amenable to thorough molecular analyses, its use as a model organism helped uncover mechanisms linking lipid metabolism and intracellular transport to complex biological processes within eukaryotic cells. The availability of a versatile analytical method for the robust, sensitive, and accurate quantitative assessment of major classes of lipids within a yeast cell is crucial for getting deep insights into these mechanisms. Here we present a protocol to use liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) for the quantitative analysis of major cellular lipids of S. cerevisiae. The LC-MS/MS method described is versatile and robust. It enables the identification and quantification of numerous species (including different isobaric or isomeric forms) within each of the 10 lipid classes. This method is sensitive and allows identification and quantitation of some lipid species at concentrations as low as 0.2 pmol/µL. The method has been successfully applied to assessing lipidomes of whole yeast cells and their purified organelles. The use of alternative mobile phase additives for electrospray ionization mass spectrometry in this method can increase the efficiency of ionization for some lipid species and can be therefore used to improve their identification and quantitation.
    MeSH term(s) Chromatography, Liquid ; Lipid Metabolism ; Lipidomics ; Lipids/isolation & purification ; Phosphatidylserines/metabolism ; Reference Standards ; Saccharomyces cerevisiae/cytology ; Saccharomyces cerevisiae/metabolism ; Spectrometry, Mass, Electrospray Ionization ; Spectrometry, Mass, Secondary Ion ; Tandem Mass Spectrometry/methods
    Chemical Substances Lipids ; Phosphatidylserines
    Language English
    Publishing date 2020-03-08
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Video-Audio Media
    ZDB-ID 2259946-0
    ISSN 1940-087X ; 1940-087X
    ISSN (online) 1940-087X
    ISSN 1940-087X
    DOI 10.3791/60616
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top