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  1. Article ; Online: γ-Cyclodextrin-polyurethane copolymer adsorbent for selective removal of endotoxin from DNA solution.

    Sakata, Masayo / Uezono, Koji / Kimura, Kasane / Todokoro, Masami

    Analytical biochemistry

    2013  Volume 443, Issue 1, Page(s) 41–45

    Abstract: Copolymer particles for removal of endotoxins (lipopolysaccharides, LPSs) were prepared by suspension copolymerization of γ-cyclodextrin (CyD) and 1,6-hexamethylenediisocyanate. The LPS-removing activity of the copolymer particles was compared with that ... ...

    Abstract Copolymer particles for removal of endotoxins (lipopolysaccharides, LPSs) were prepared by suspension copolymerization of γ-cyclodextrin (CyD) and 1,6-hexamethylenediisocyanate. The LPS-removing activity of the copolymer particles was compared with that of poly(ε-lysine)-immobilized Cellufine (cationic adsorbent) or polystyrene particles (hydrophobic adsorbent) by a batch method. When DNA was present in solution with LPSs under physiological conditions (pH 6.0, ionic strength of μ = 0.05-0.8), LPS-removing activity of the cationic or hydrophobic adsorbent was unsatisfactory because both the DNA and the LPSs were adsorbed onto each adsorbent. By contrast, the copolymer particles with γ-CyD cavity (CyD content: 14-20 mol%) could selectively remove LPSs from a DNA solution (50 μg ml(-1), pH 6.0, and μ = 0.05-0.2) containing LPSs (15 EU ml(-1)) without the adsorption of DNA. The residual concentration of LPSs in the treated DNA solution was below 0.1 EU ml(-1), and the recovery of DNA was 99%.
    MeSH term(s) Adsorption ; Animals ; Cyanates/chemistry ; DNA/isolation & purification ; Escherichia coli/chemistry ; Hydrogen-Ion Concentration ; Isocyanates ; Lipopolysaccharides/isolation & purification ; Male ; Microspheres ; Osmolar Concentration ; Polymerization ; Polyurethanes/chemistry ; Salmon ; Sensitivity and Specificity ; Solid Phase Extraction ; Solutions ; Spectroscopy, Fourier Transform Infrared ; Spermatozoa/chemistry ; gamma-Cyclodextrins/chemistry
    Chemical Substances Cyanates ; Isocyanates ; Lipopolysaccharides ; Polyurethanes ; Solutions ; gamma-Cyclodextrins ; 1,6-hexamethylene diisocyanate (0I70A3I1UF) ; DNA (9007-49-2) ; gamma-cyclodextrin (KZJ0BYZ5VA)
    Language English
    Publishing date 2013-12-01
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1110-1
    ISSN 1096-0309 ; 0003-2697
    ISSN (online) 1096-0309
    ISSN 0003-2697
    DOI 10.1016/j.ab.2013.08.010
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 389: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: Limulus amebocyte lysate assay for endotoxins by an adsorption method with polycation-immobilized cellulose beads.

    Sakata, Masayo / Inoue, Tomofumi / Todokoro, Masami / Kunitake, Masashi

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

    2010  Volume 26, Issue 3, Page(s) 291–296

    Abstract: To assay lipopolysaccharides (LPSs) in solutions containing Limulus amebocyte lysate (LAL)-inhibiting or LAL-enhancing compounds, we developed a selective endotoxin (LPS) assay using poly(epsilon-lysine)-immobilized cellulose beads (PL-Cellufine) and LAL. ...

    Abstract To assay lipopolysaccharides (LPSs) in solutions containing Limulus amebocyte lysate (LAL)-inhibiting or LAL-enhancing compounds, we developed a selective endotoxin (LPS) assay using poly(epsilon-lysine)-immobilized cellulose beads (PL-Cellufine) and LAL. The PL-Cellufine can adsorb LPSs in a solution containing certain compounds (NaCl, proteins and amino acids) at an ionic strength of mu = 0.05-0.4 at neutral pH. The LPSs adsorbed on the PL-Cellufine were separated from the compounds by centrifugation and then the PL-Cellufine was suspended in LPS-free water. The LPS activities of the suspension are directly assayed by a turbidimetric time assay with the LAL reagent. The accuracy of the adsorption method was high compared with those of common solution methods. As for the common method, the apparent recovery of LPS from the compounds was 40-95%. This suggests that these compounds inhibit the LAL procedure. By contrast, the adsorption method showed good LPS recovery (88-120%) in all cases, without being inhibited or enhanced by the compounds.
    MeSH term(s) Adsorption ; Cations/chemistry ; Cellulose/chemistry ; Limulus Test/methods ; Lipopolysaccharides/analysis ; Microspheres ; Osmolar Concentration ; Polylysine/chemistry ; Surface Properties
    Chemical Substances Cations ; Lipopolysaccharides ; Polylysine (25104-18-1) ; Cellulose (9004-34-6)
    Language English
    Publishing date 2010-03-08
    Publishing country Japan
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1483376-1
    ISSN 1348-2246 ; 0910-6340
    ISSN (online) 1348-2246
    ISSN 0910-6340
    DOI 10.2116/analsci.26.291
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Selective assay for endotoxin using poly(epsilon-lysine)-immobilized Cellufine and Limulus amoebocyte lysate (LAL).

    Sakata, Masayo / Fukuma, Yuriko / Todokoro, Masami / Kunitake, Masashi

    Analytical biochemistry

    2009  Volume 385, Issue 2, Page(s) 368–370

    Abstract: We developed a selective endotoxin (lipopolysaccharide; LPS) assay using poly(epsilon-lysine)-immobilized cellulose beads (PL-Cellufine) and Limulus amoebocyte lysate (LAL). First, LPS was selectively adsorbed on the beads in a solution containing ... ...

    Abstract We developed a selective endotoxin (lipopolysaccharide; LPS) assay using poly(epsilon-lysine)-immobilized cellulose beads (PL-Cellufine) and Limulus amoebocyte lysate (LAL). First, LPS was selectively adsorbed on the beads in a solution containing various LAL-inhibiting or LAL-enhancing compounds (e.g., amino acids, enzymes) and the LPS adsorbed on the beads was separated from the compounds by centrifugation. Second, the LPS adsorbed on the beads directly reacted with the LAL reagent, and the LPS concentration was determined by a turbidimetric time assay. The accuracy of the adsorption method with PL-Cellufine was high compared with that of a common solution method. Apparent recovery of LPS from compound solution was 88-120%.
    MeSH term(s) Adsorption ; Animals ; Antimicrobial Cationic Peptides ; Arthropod Proteins ; Endotoxins/analysis ; Invertebrate Hormones/chemistry ; Lipopolysaccharides ; Membrane Proteins ; Microspheres ; Nephelometry and Turbidimetry ; Polylysine/chemistry ; Research Design
    Chemical Substances Antimicrobial Cationic Peptides ; Arthropod Proteins ; Endotoxins ; Invertebrate Hormones ; Lipopolysaccharides ; Membrane Proteins ; antilipopolysaccharide factor (Limulus) ; endotoxin binding proteins ; Polylysine (25104-18-1)
    Language English
    Publishing date 2009-02-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1110-1
    ISSN 1096-0309 ; 0003-2697
    ISSN (online) 1096-0309
    ISSN 0003-2697
    DOI 10.1016/j.ab.2008.11.002
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 389: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Selective removal of endotoxin from a DNA solution by cross-linked cyclodextrin beads.

    Sakata, Masayo / Yoshimura, Kana / Sakamoto, Itsumi / Todokoro, Masami / Kunitake, Masashi

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

    2011  Volume 27, Issue 2, Page(s) 213–216

    Abstract: The removal of lipopolysaccharide (LPS) from a contaminated DNA solution was achieved using cross-linked cyclodextrin (CyD polymer) beads as LPS adsorbents. The LPS-removing activity of the β- and γ-CyD polymer beads was compared with that of common ... ...

    Abstract The removal of lipopolysaccharide (LPS) from a contaminated DNA solution was achieved using cross-linked cyclodextrin (CyD polymer) beads as LPS adsorbents. The LPS-removing activity of the β- and γ-CyD polymer beads was compared with that of common cationic LPS adsorbents. The γ-CyD polymer beads selectively removed LPS from a DNA solution (50 µg mL(-1), pH 6, ionic strength μ = 0.2) containing natural LPS (15 EU mL(-1)), without the adsorption of DNA. The adsorptions of LPS and DNA were 85% and <1%, respectively.
    MeSH term(s) Adsorption ; Buffers ; DNA/chemistry ; Hydrogen-Ion Concentration ; Lipopolysaccharides/chemistry ; Lipopolysaccharides/isolation & purification ; Microspheres ; Osmolar Concentration ; Solutions ; beta-Cyclodextrins/chemistry ; gamma-Cyclodextrins/chemistry
    Chemical Substances Buffers ; Lipopolysaccharides ; Solutions ; beta-Cyclodextrins ; gamma-Cyclodextrins ; DNA (9007-49-2)
    Language English
    Publishing date 2011-01-20
    Publishing country Japan
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1483376-1
    ISSN 1348-2246 ; 0910-6340
    ISSN (online) 1348-2246
    ISSN 0910-6340
    DOI 10.2116/analsci.27.213
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Purification and concentration of non-infectious West Nile virus-like particles and infectious virions using a pseudo-affinity Cellufine Sulfate column.

    Ohtaki, Naohiro / Takahashi, Hidehiro / Kaneko, Keiko / Gomi, Yasuyuki / Ishikawa, Toyokazu / Higashi, Yasushi / Todokoro, Masami / Kurata, Takeshi / Sata, Tetsutaro / Kojima, Asato

    Journal of virological methods

    2011  Volume 174, Issue 1-2, Page(s) 131–135

    Abstract: Affinity column chromatography is a promising method for the purification of flavivirus particles that can supplement or potentially replace diafiltration and sucrose density centrifugation. In this study, the purification of West Nile Virus (WNV) ... ...

    Abstract Affinity column chromatography is a promising method for the purification of flavivirus particles that can supplement or potentially replace diafiltration and sucrose density centrifugation. In this study, the purification of West Nile Virus (WNV) antigens via Cellufine Sulfate column chromatography was examined. Virus-like particles (VLPs) produced by the expression of the prM and E genes were separated from most of the contaminant proteins with 0.2-0.4M NaCl, but still retained their spherical forms and immunogenicity in mice. The column, with a 1 mL bed-volume, concentrated WN-VLPs a minimum of 15 fold from culture supernatants. A heparin analogue, suramin, competitively eluted WN-VLPs, but sulphated polysaccharides, such as heparin, heparin sulfate and dextran sulfate, did not. Furthermore, 2.4 × 10⁹ plaque forming units of WNV and 196 μg of the viral antigens were recovered from 60 mL of infected culture medium at high yields (93% and 96%, respectively). These results indicate that, in addition to conventional methods, Cellufine Sulfate column chromatography is an effective preparation technique for WNV particulate antigens that does not impair the antigen virological characteristics.
    MeSH term(s) Animals ; Chromatography, Affinity/methods ; Chromatography, Liquid/methods ; Mice ; Virion/isolation & purification ; Virology/methods ; Virosomes/isolation & purification ; West Nile virus/isolation & purification
    Chemical Substances Virosomes
    Language English
    Publishing date 2011-06
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 8013-5
    ISSN 1879-0984 ; 0166-0934
    ISSN (online) 1879-0984
    ISSN 0166-0934
    DOI 10.1016/j.jviromet.2011.03.021
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1565: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  6. Article: γ-Cyclodextrin–polyurethane copolymer adsorbent for selective removal of endotoxin from DNA solution

    Sakata, Masayo / Uezono, Koji / Kimura, Kasane / Todokoro, Masami

    Analytical biochemistry

    Volume v. 443,, Issue no. 1

    Abstract: Copolymer particles for removal of endotoxins (lipopolysaccharides, LPSs) were prepared by suspension copolymerization of γ-cyclodextrin (CyD) and 1,6-hexamethylenediisocyanate. The LPS-removing activity of the copolymer particles was compared with that ... ...

    Abstract Copolymer particles for removal of endotoxins (lipopolysaccharides, LPSs) were prepared by suspension copolymerization of γ-cyclodextrin (CyD) and 1,6-hexamethylenediisocyanate. The LPS-removing activity of the copolymer particles was compared with that of poly(ε-lysine)-immobilized Cellufine (cationic adsorbent) or polystyrene particles (hydrophobic adsorbent) by a batch method. When DNA was present in solution with LPSs under physiological conditions (pH 6.0, ionic strength of μ=0.05–0.8), LPS-removing activity of the cationic or hydrophobic adsorbent was unsatisfactory because both the DNA and the LPSs were adsorbed onto each adsorbent. By contrast, the copolymer particles with γ-CyD cavity (CyD content: 14–20mol%) could selectively remove LPSs from a DNA solution (50μgml⁻¹, pH 6.0, and μ=0.05–0.2) containing LPSs (15EUml⁻¹) without the adsorption of DNA. The residual concentration of LPSs in the treated DNA solution was below 0.1EUml⁻¹, and the recovery of DNA was 99%.
    Keywords endotoxins ; polystyrenes ; gamma-cyclodextrin ; DNA ; composite polymers ; adsorbents ; adsorption ; hydrophobicity ; lipopolysaccharides ; pH ; ionic strength
    Language English
    Document type Article
    ISSN 0003-2697
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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  7. Article: Purification and concentration of non-infectious West Nile virus-like particles and infectious virions using a pseudo-affinity Cellufine Sulfate column

    Ohtaki, Naohiro / Takahashi, Hidehiro / Kaneko, Keiko / Gomi, Yasuyuki / Ishikawa, Toyokazu / Higashi, Yasushi / Todokoro, Masami / Kurata, Takeshi / Sata, Tetsutaro / Kojima, Asato

    Journal of virological methods

    Volume v. 174,, Issue no. 1

    Abstract: Affinity column chromatography is a promising method for the purification of flavivirus particles that can supplement or potentially replace diafiltration and sucrose density centrifugation. In this study, the purification of West Nile Virus (WNV) ... ...

    Abstract Affinity column chromatography is a promising method for the purification of flavivirus particles that can supplement or potentially replace diafiltration and sucrose density centrifugation. In this study, the purification of West Nile Virus (WNV) antigens via Cellufine Sulfate column chromatography was examined. Virus-like particles (VLPs) produced by the expression of the prM and E genes were separated from most of the contaminant proteins with 0.2–0.4M NaCl, but still retained their spherical forms and immunogenicity in mice. The column, with a 1mL bed-volume, concentrated WN-VLPs a minimum of 15 fold from culture supernatants. A heparin analogue, suramin, competitively eluted WN-VLPs, but sulphated polysaccharides, such as heparin, heparin sulfate and dextran sulfate, did not. Furthermore, 2.4×10⁹ plaque forming units of WNV and 196μg of the viral antigens were recovered from 60mL of infected culture medium at high yields (93% and 96%, respectively). These results indicate that, in addition to conventional methods, Cellufine Sulfate column chromatography is an effective preparation technique for WNV particulate antigens that does not impair the antigen virological characteristics.
    Keywords virion ; chromatography ; sodium chloride ; viral antigens ; sulfates ; centrifugation ; dextran ; genes ; sucrose ; mice ; virus-like particles ; suramin ; purification methods ; culture media ; heparin ; immune response ; proteins ; West Nile virus
    Language English
    Document type Article
    ISSN 0166-0934
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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