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  1. Article ; Online: Tumor Tissue Oxidative Stress Changes and Na, K-ATPase Evaluation in Head and Neck Squamous Cell Carcinoma.

    Toledo, Marina Marques / De Souza Gonçalves, Bruno / Colodette, Natalie Mounteer / Chaves, Aline Lauda Freitas / Muniz, Luciana Vieira / De A Ribeiro, Rosy Iara Maciel / Dos Santos, Hélio Batista / Cortes, Vanessa F / Soares, Joao Marcos Arantes / De Lima Santos, Hérica / Barbosa, Leandro A

    The Journal of membrane biology

    2021  Volume 254, Issue 5-6, Page(s) 475–486

    Abstract: Changes in metabolism are mechanisms that are largely implicated in the development, progression, and metastasis of head and neck squamous cell carcinoma (HNSCC) and also in resistance to different anticancer therapies. Identification of biomarkers for ... ...

    Abstract Changes in metabolism are mechanisms that are largely implicated in the development, progression, and metastasis of head and neck squamous cell carcinoma (HNSCC) and also in resistance to different anticancer therapies. Identification of biomarkers for differentiation between cancerous and normal epithelium, treatment design and prognosis remain a vital issue in the field of head and neck cancer. The present study analyzed the main biochemical changes that occur in HNSCC tumors by through mechanisms involving oxidative stress. The release of substances reactive to thiobarbituric acid was significantly lower in HNSCC tumor tissue as compared to healthy tissue. The assays related to the lipid profile assays showed changes in membrane biophysics of tumor cells due to an increase in total phospholipids and total cholesterol, as well as an increased activity and expression of the α1 subunit of Na, K-ATPase, which is fundamental in the process of carcinogenesis. The modulation of the antioxidant system was also affected, with a decrease in the catalytic activity of the enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx), as well as a reduction of glutathione (GSH) content and an increase in H
    MeSH term(s) Adenosine Triphosphatases ; Head and Neck Neoplasms ; Humans ; Hydrogen Peroxide ; Oxidative Stress ; Squamous Cell Carcinoma of Head and Neck
    Chemical Substances Hydrogen Peroxide (BBX060AN9V) ; Adenosine Triphosphatases (EC 3.6.1.-)
    Language English
    Publishing date 2021-06-08
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 3082-x
    ISSN 1432-1424 ; 0022-2631
    ISSN (online) 1432-1424
    ISSN 0022-2631
    DOI 10.1007/s00232-021-00185-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 613: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: Evaluation of Cardiotonic Steroid Modulation of Cellular Cholesterol and Phospholipid.

    Silva, Lilian N D / Garcia, Israel J P / Valadares, Jessica M M / Pessoa, Marco Tulio C / Toledo, Marina Marques / Machado, Matheus V / Busch, Mileane Souza / Rocha, Isabella / Villar, José Augusto F P / Atella, Georgia C / Santos, Herica L / Cortes, Vanessa F / Barbosa, Leandro A

    The Journal of membrane biology

    2021  Volume 254, Issue 5-6, Page(s) 499–512

    Abstract: We have previously shown that 21-benzylidene digoxin (21-BD) increases the total cholesterol and phospholipid content on the membrane of HeLa cells. Lipid modulation caused by cardiotonic steroids (CTS) is still unexplored. Therefore, the aim of the ... ...

    Abstract We have previously shown that 21-benzylidene digoxin (21-BD) increases the total cholesterol and phospholipid content on the membrane of HeLa cells. Lipid modulation caused by cardiotonic steroids (CTS) is still unexplored. Therefore, the aim of the present study was to evaluate the cholesterol and phospholipid modulation of the cell membrane caused by ouabain and 21-BD and the possible involvement of the caveolae on this modulation. For this, one cell line containing caveolae (HeLa) and other not containing (Caco-2) were used. The modulation of the lipid profile was evaluated by total cholesterol and phospholipids measurements, and identification of membrane phospholipids by HPTLC. The cholesterol distribution was evaluated by filipin staining. The caveolin-1 expression was evaluated by Western Blotting. Ouabain had no effect on the total membrane lipid content in both cell lines. However, 21-BD increased total membrane phospholipid content and had no effect on the membrane cholesterol content in Caco-2 cells. CTS were not able to alter the specific phospholipids content. In the filipin experiments, 21-BD provoked a remarkable redistribution of cholesterol to the perinuclear region of HeLa cells. In Caco-2 cells, it was observed only a slight increase in cholesterol, especially as intracellular vesicles. The caveolin-1 expression was not altered by any of the compounds. Our data mainly show different effects of two cardiotonic steroids. Ouabain had no effect on the lipid profile of cells, whereas 21-BD causes important changes in cholesterol and phospholipid content. Therefore, the modulation of cholesterol content in the plasma membrane of HeLa cells is not correlated with the expression of caveolin-1.
    MeSH term(s) Caco-2 Cells ; Cardiac Glycosides/metabolism ; Caveolin 1 ; Cholesterol ; Filipin ; HeLa Cells ; Humans ; Ouabain/pharmacology ; Phospholipids
    Chemical Substances Cardiac Glycosides ; Caveolin 1 ; Phospholipids ; Ouabain (5ACL011P69) ; Filipin (87Z59R7D14) ; Cholesterol (97C5T2UQ7J)
    Language English
    Publishing date 2021-10-29
    Publishing country United States
    Document type Evaluation Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 3082-x
    ISSN 1432-1424 ; 0022-2631
    ISSN (online) 1432-1424
    ISSN 0022-2631
    DOI 10.1007/s00232-021-00203-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 613: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: Evaluation of the Erythrocyte Membrane in Head and Neck Cancer Patients.

    De Souza Gonçalves, Bruno / Toledo, Marina Marques / Colodette, Natalie Mounteer / Chaves, Aline Lauda Freitas / Muniz, Luciana Vieira / Ribeiro, Rosy Iara Maciel De A / Dos Santos, Hélio Batista / Cortes, Vanessa F / Soares, João Marcos Arantes / Barbosa, Leandro A / de Lima Santos, Hérica

    The Journal of membrane biology

    2020  Volume 253, Issue 6, Page(s) 617–629

    Abstract: Head and neck squamous cell carcinoma (HNSCC) is a heterogeneous and complex disease, both from a clinical and molecular point of view. The prolonged use of alcohol and tobacco, along with the release of tumor secretions can modulate blood cells, such as ...

    Abstract Head and neck squamous cell carcinoma (HNSCC) is a heterogeneous and complex disease, both from a clinical and molecular point of view. The prolonged use of alcohol and tobacco, along with the release of tumor secretions can modulate blood cells, such as erythrocytes. Here, this study was conducted with 24 patients diagnosed with HNSCC and an equal number of healthy individuals are matched by age and gender. The levels of lipid peroxidation were measured using the individual plasma, while for lipid concentrations, identification and quantification Na, K-ATPase activity and osmotic fragility, the red blood cell concentrate were used. The release of TBARS was significantly higher in patients with HNSCC. The lipid profile assays demonstrated a rearrangement of the erythrocyte membrane due to a decrease in total phospholipids and phosphatidylethanolamine followed by an increase in total cholesterol and phosphatidylcholine. Na, K-ATPase activity also increased. Erythrocytes were more fragile in patients with HNSCC than in health individuals. Therefore, the membrane of erythrocytes were rearranged and Na, K-ATPase function altered in the HNSCC patients. Our findings suggests that the alcohol, tobacco and tumor secretion modulate in a specific manner that the erythrocytes membranes of these patients making this system a potential tool for HNSCC biomarker of tumor progression.
    MeSH term(s) Biomarkers ; Case-Control Studies ; Erythrocyte Membrane/chemistry ; Erythrocyte Membrane/metabolism ; Head and Neck Neoplasms/metabolism ; Humans ; Lipid Peroxidation ; Membrane Lipids/metabolism ; Osmotic Fragility ; Oxidative Stress ; Sodium-Potassium-Exchanging ATPase/metabolism
    Chemical Substances Biomarkers ; Membrane Lipids ; Sodium-Potassium-Exchanging ATPase (EC 7.2.2.13)
    Language English
    Publishing date 2020-10-22
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 3082-x
    ISSN 1432-1424 ; 0022-2631
    ISSN (online) 1432-1424
    ISSN 0022-2631
    DOI 10.1007/s00232-020-00147-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 613: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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