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  1. Article ; Online: Neutrophil Extracellular Traps Associate with Clinical Stages in Breast Cancer.

    Rivera-Franco, Monica M / Leon-Rodriguez, Eucario / Torres-Ruiz, José J / Gómez-Martín, Diana / Angles-Cano, Eduardo / de la Luz Sevilla-González, María

    Pathology oncology research : POR

    2019  Volume 26, Issue 3, Page(s) 1781–1785

    Abstract: Recently, neutrophil extracellular traps (NETs), three-dimensional structures formed of neutrophil enzymes such as neutrophil elastase (NE) and nuclear components (DNA), have been associated with progression in different types of cancer. However, data ... ...

    Abstract Recently, neutrophil extracellular traps (NETs), three-dimensional structures formed of neutrophil enzymes such as neutrophil elastase (NE) and nuclear components (DNA), have been associated with progression in different types of cancer. However, data remain scarce in breast cancer. Thus, the aim of this study was to associate NETs with clinical stages of breast cancer. A prospective analysis was performed in 45 plasma samples of female patients with newly diagnosed breast cancer. NE-DNA complexes were evaluated by ELISA. Optical density was dichotomized at the median for comparisons (low and high levels of NE-DNA). The most frequent clinical stage was localized (n = 28, 62%) followed by regional (n = 13, 29%) and distant (n = 4, 9%). Higher levels of NE-DNA complexes were observed in regional and distant stages compared to localized disease (68% vs 32%, p = 0.034). No differences were observed when comparing other clinical characteristics between both groups. We demonstrated that the levels of NETs increase in proportion to the stage of the disease, observing higher levels of NE-DNA complexes in regional and metastatic disease, which coincides with the proposed mechanism by which cancer progression and metastasis might result from the formation of NETs.
    MeSH term(s) Adult ; Aged ; Aged, 80 and over ; Breast Neoplasms/immunology ; Breast Neoplasms/pathology ; Disease Progression ; Extracellular Traps/immunology ; Female ; Humans ; Middle Aged ; Neoplasm Staging
    Language English
    Publishing date 2019-10-27
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1375979-6
    ISSN 1532-2807 ; 1219-4956
    ISSN (online) 1532-2807
    ISSN 1219-4956
    DOI 10.1007/s12253-019-00763-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4936: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article: Sarcoidosis and Autoimmunity: From Genetic Background to Environmental Factors.

    Bindoli, Sara / Dagan, Amir / Torres-Ruiz, José J / Perricone, Carlo / Bizjak, Mojca / Doria, Andrea / Shoenfeld, Yehuda

    The Israel Medical Association journal : IMAJ

    2016  Volume 18, Issue 3-4, Page(s) 197–202

    Abstract: Sarcoidosis is a chronic multisystem disease with variable course resulting from the interaction between environmental factors and the immune system of individuals genetically predisposed. The evidence linking sarcoidosis with environmental triggers such ...

    Abstract Sarcoidosis is a chronic multisystem disease with variable course resulting from the interaction between environmental factors and the immune system of individuals genetically predisposed. The evidence linking sarcoidosis with environmental triggers such as metals is increasing. We describe the case of a 44 year old female with a history. of smoking since age 30 and previous mercury dental filling who presented at physical examination with numerous subcutaneous nodules. Laboratory data showed accelerated erythrocyte sedimentation rate and high titer of anti-U1 ribonucleoprotein antibodies (U1 RNP). Skin biopsy and chest X-ray suggested the diagnosis of sarcoidosis. In this report we illustrate the different causes involved in the onset of sarcoidosis.
    MeSH term(s) Adult ; Autoantibodies ; Autoimmunity/genetics ; Blood Sedimentation ; Dental Amalgam/therapeutic use ; Female ; Humans ; Pedigree ; Ribonucleoproteins/immunology ; Sarcoidosis, Pulmonary/complications ; Sarcoidosis, Pulmonary/diagnosis ; Sarcoidosis, Pulmonary/immunology ; Sarcoidosis, Pulmonary/physiopathology ; Skin Diseases/diagnosis ; Skin Diseases/etiology ; Smoking/adverse effects
    Chemical Substances Autoantibodies ; Ribonucleoproteins ; Dental Amalgam (8049-85-2)
    Language English
    Publishing date 2016-03
    Publishing country Israel
    Document type Case Reports ; Journal Article
    ZDB-ID 2008291-5
    ISSN 1565-1088 ; 0021-2180
    ISSN 1565-1088 ; 0021-2180
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5470: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article: Recombinant Protein Expression and Purification of N, S1, and RBD of SARS-CoV-2 from Mammalian Cells and Their Potential Applications.

    García-Cordero, Julio / Mendoza-Ramírez, Juvenal / Fernández-Benavides, David / Roa-Velazquez, Daniela / Filisola-Villaseñor, Jessica / Martínez-Frías, Sandra Paola / Sanchez-Salguero, Erik Saul / Miguel-Rodríguez, Carlos E / Maravillas Montero, Jose L / Torres-Ruiz, Jose J / Gómez-Martín, Diana / Argumedo, Leopoldo Santos / Morales-Ríos, Edgar / Alvarado-Orozco, Juan M / Cedillo-Barrón, Leticia

    Diagnostics (Basel, Switzerland)

    2021  Volume 11, Issue 10

    Abstract: The coronavirus disease 2019 (COVID-19) pandemic has reached an unprecedented level. There is a strong demand for diagnostic and serological supplies worldwide, making it necessary for countries to establish their own technologies to produce high-quality ...

    Abstract The coronavirus disease 2019 (COVID-19) pandemic has reached an unprecedented level. There is a strong demand for diagnostic and serological supplies worldwide, making it necessary for countries to establish their own technologies to produce high-quality biomolecules. The two main viral antigens used for the diagnostics for severe acute respiratory syndrome coronavirus (SARS-CoV-2) are the structural proteins spike (S) protein and nucleocapsid (N) protein. The spike protein of SARS-CoV-2 is cleaved into S1 and S2, in which the S1 subunit has the receptor-binding domain (RBD), which induces the production of neutralizing antibodies, whereas nucleocapsid is an ideal target for viral antigen-based detection. In this study, we designed plasmids, pcDNA3.1/S1 and pcDNA3.1/N, and optimized their expression of the recombinant S1 and N proteins from SARS-CoV-2 in a mammalian system. The RBD was used as a control. The antigens were successfully purified from Expi293 cells, with high yields of the S1, N, and RBD proteins. The immunogenic abilities of these proteins were demonstrated in a mouse model. Further, enzyme-linked immunosorbent assays with human serum samples showed that the SARS-CoV-2 antigens are a suitable alternative for serological assays to identify patients infected with COVID-19.
    Language English
    Publishing date 2021-09-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662336-5
    ISSN 2075-4418
    ISSN 2075-4418
    DOI 10.3390/diagnostics11101808
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Severe COVID-19 is marked by dysregulated serum levels of carboxypeptidase A3 and serotonin.

    Soria-Castro, Rodolfo / Meneses-Preza, Yatsiri G / Rodríguez-López, Gloria M / Romero-Ramírez, Sandra / Sosa-Hernández, Víctor A / Cervantes-Díaz, Rodrigo / Pérez-Fragoso, Alfredo / Torres-Ruíz, José J / Gómez-Martín, Diana / Campillo-Navarro, Marcia / Álvarez-Jiménez, Violeta D / Pérez-Tapia, Sonia M / Chávez-Blanco, Alma D / Estrada-Parra, Sergio / Maravillas-Montero, José L / Chacón-Salinas, Rommel

    Journal of leukocyte biology

    2021  Volume 110, Issue 3, Page(s) 425–431

    Abstract: The immune response plays a critical role in the pathophysiology of SARS-CoV-2 infection ranging from protection to tissue damage and all occur in the development of acute respiratory distress syndrome (ARDS). ARDS patients display elevated levels of ... ...

    Abstract The immune response plays a critical role in the pathophysiology of SARS-CoV-2 infection ranging from protection to tissue damage and all occur in the development of acute respiratory distress syndrome (ARDS). ARDS patients display elevated levels of inflammatory cytokines and innate immune cells, and T and B cell lymphocytes have been implicated in this dysregulated immune response. Mast cells are abundant resident cells of the respiratory tract and are able to release different inflammatory mediators rapidly following stimulation. Recently, mast cells have been associated with tissue damage during viral infections, but their role in SARS-CoV-2 infection remains unclear. In this study, we examined the profile of mast cell activation markers in the serum of COVID-19 patients. We noticed that SARS-CoV-2-infected patients showed increased carboxypeptidase A3 (CPA3) and decreased serotonin levels in their serum when compared with symptomatic SARS-CoV-2-negative patients. CPA3 levels correlated with C-reactive protein, the number of circulating neutrophils, and quick SOFA. CPA3 in serum was a good biomarker for identifying severe COVID-19 patients, whereas serotonin was a good predictor of SARS-CoV-2 infection. In summary, our results show that serum CPA3 and serotonin levels are relevant biomarkers during SARS-CoV-2 infection. This suggests that mast cells and basophils are relevant players in the inflammatory response in COVID-19 and may represent targets for therapeutic intervention.
    MeSH term(s) Biomarkers/analysis ; COVID-19/complications ; COVID-19/diagnosis ; COVID-19/metabolism ; COVID-19/virology ; Carboxypeptidases A/metabolism ; Humans ; Inflammation/diagnosis ; Inflammation/etiology ; Inflammation/metabolism ; Inflammation/pathology ; Inflammation Mediators/metabolism ; Mast Cells/immunology ; Mast Cells/pathology ; SARS-CoV-2/isolation & purification ; Serotonin/metabolism ; Severity of Illness Index
    Chemical Substances Biomarkers ; Inflammation Mediators ; Serotonin (333DO1RDJY) ; CPA3 protein, human (EC 3.4.17.1) ; Carboxypeptidases A (EC 3.4.17.1)
    Language English
    Publishing date 2021-05-31
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 605722-6
    ISSN 1938-3673 ; 0741-5400
    ISSN (online) 1938-3673
    ISSN 0741-5400
    DOI 10.1002/JLB.4HI0221-087R
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 603: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
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    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: SEVERE COVID-19 IS MARKED BY DYSREGULATED SERUM LEVELS OF CARBOXYPEPTIDASE A3 AND SEROTONIN

    Soria-Castro, Rodolfo / Meneses-Preza, Yatsiri G. / Rodriguez-Lopez, Gloria M. / Romero-Ramirez, Sandra / Sosa-Hernandez, Victor A. / Cervantes-Diaz, Rodrigo / Perez-Fragoso, Alfredo / Torres-Ruiz, Jose J / Gomez-Martin, Diana / Campillo-Navarro, Marcia / Alvarez-Jimenez, Violeta D. / Perez-Tapia, Sonia M. / Chavez-Blanco, Alma D. / Estrada-Parra, Sergio / Maravillas-Montero, Jose L. / Chacon-Salinas, Rommel

    medRxiv

    Abstract: The immune response plays a critical role in the pathophysiology of SARS-CoV-2 infection ranging from protection to tissue damage. This is observed in the development of acute respiratory distress syndrome when elevated levels of inflammatory cytokines ... ...

    Abstract The immune response plays a critical role in the pathophysiology of SARS-CoV-2 infection ranging from protection to tissue damage. This is observed in the development of acute respiratory distress syndrome when elevated levels of inflammatory cytokines are detected. Several cells of the immune response are implied in this dysregulated immune response including innate immune cells and T and B cell lymphocytes. Mast cells are abundant resident cells of the respiratory tract, able to rapidly release different inflammatory mediators following stimulation. Recently, mast cells have been associated with tissue damage during viral infections, but little is known about their role in SARS-CoV-2 infection. In this study we examined the profile of mast cell activation markers in the serum of COVID-19 patients. We noticed that SARS-CoV-2 infected patients showed increased carboxypeptidase A3 (CPA3), and decreased serotonin levels in their serum. CPA3 levels correlated with C-reactive protein, the number of circulating neutrophils and quick SOFA. CPA3 in serum was a good biomarker for identifying severe COVID-19 patients, while serotonin was a good predictor of SARS-CoV-2 infection. In summary, our results show that serum CPA3 and serotonin levels are relevant biomarkers during SARS-CoV-2 infection, suggesting that mast cells are relevant players in the inflammatory response in COVID-19, might represent targets for therapeutic intervention.
    Keywords covid19
    Language English
    Publishing date 2021-02-03
    Publisher Cold Spring Harbor Laboratory Press
    Document type Article ; Online
    DOI 10.1101/2021.02.02.21251020
    Database COVID19

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