Article ; Online: Skin rejuvenation and photoaging protection using adipose-derived stem cell extracellular vesicles loaded with exogenous cargos.
2024 Volume 30, Issue 2, Page(s) e13599
Abstract: Background: Small extracellular vesicles from adipose-derived stem cells (ASC-sEVs) have gained remarkable attention for their regenerative and protective properties against skin aging. However, the use of ASC-sEVs to further encapsulate certain natural ...
| Abstract | Background: Small extracellular vesicles from adipose-derived stem cells (ASC-sEVs) have gained remarkable attention for their regenerative and protective properties against skin aging. However, the use of ASC-sEVs to further encapsulate certain natural anti-aging compounds for synergistic effects has not been actively explored. For large-scale production in skincare industry, it is also crucial to standardize cost-effective methods to produce highly pure ASC-sEVs. Methods: Human ASCs were expanded in serum-free media with different compositions to first optimize the sEV production. ASC-sEVs from different batches were then purified using tangential flow filtration and sucrose cushion ultracentrifugation, followed by extensive characterization for identity and content profiling including proteomics, lipidomics and miRNA sequencing. ASC-sEVs were further loaded with nicotinamide riboside (NR) and resveratrol by sonication-incubation method. The therapeutic effect of ASC-sEVs and loaded ASC-sEVs was tested on human keratinocyte cell line HaCaT exposed to UVB by measuring reactive oxygen species (ROS). The loaded ASC-sEVs were later applied on the hand skin of three volunteers once a day for 8 weeks and skin analysis was performed every 2 weeks. Results: Our standardized workflow produced ASC-sEVs with high yield, high purity and with stable characteristics and consistent biocargo among different batches. The most abundant subpopulations in ASC-sEVs were CD63 Conclusions: This study demonstrated a robust protocol to produce ASC-sEVs and exogenously load them with natural compounds. The loaded ASC-sEVs exhibited synergistic effects of both sEVs and anti-aging compounds in photoaging protection and skin rejuvenation. |
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| MeSH term(s) | Humans ; Skin Aging ; Reactive Oxygen Species ; Rejuvenation ; Resveratrol ; Stem Cells |
| Chemical Substances | Reactive Oxygen Species ; Resveratrol (Q369O8926L) |
| Language | English |
| Publishing date | 2024-01-26 |
| Publishing country | England |
| Document type | Journal Article |
| ZDB-ID | 1229160-2 |
| ISSN | 1600-0846 ; 0909-752X ; 1397-1344 |
| ISSN (online) | 1600-0846 |
| ISSN | 0909-752X ; 1397-1344 |
| DOI | 10.1111/srt.13599 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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