LIVIVO - Search results -

Search results

Result 1 - 10 of total 31

Search options

Article: Strukturbezogene religionspsychologische Diagnostik

Trettin, Alexander

Trauma & Gewalt

2022 Volume 16, Issue 3, Page(s) 218–230

Abstract: Auf der Basis des Formates der strukturbezogenen Religionspsychologie wurde das Verfahren der strukturbezogenen religionspsychologischen Diagnostik entwickelt, bei dem verschiedene Erhebungs- und Analysemethoden im Sinne des Methodenpluralismus (Mixed ... ...

Title translation	Structurally related religion-psychological diagnostics
Abstract	Auf der Basis des Formates der strukturbezogenen Religionspsychologie wurde das Verfahren der strukturbezogenen religionspsychologischen Diagnostik entwickelt, bei dem verschiedene Erhebungs- und Analysemethoden im Sinne des Methodenpluralismus (Mixed Methods) je nach Bedarf miteinander kombiniert werden können. In diesem Artikel soll dieses Verfahren vorgestellt und durch einzelne Fallbeispiele aus der therapeutischen Arbeit mit traumatisierten Geflüchteten kontrastiert werden. Für die vorliegende Studie, bei der vier männliche Probanden befragt wurden, kamen folgende Methoden zum Einsatz: zur Erfassung der Religiosität bzw. der Spiritualität die Zentralitätsskala, zur Anamnese und Exploration das narrativ-diagnostische Interview, für die Auswertung die Narrationsanalyse sowie die diagnostische Bilanz (OPD-2) und zur Erfassung von Traumasymptomen die Impact of Event Scale (IES-R)
Keywords	Diagnosis ; Diagnostik ; Erzählungen ; Flüchtlinge ; Interviews ; Narratives ; Refugees ; Religion ; Religiosity ; Religiosität ; Spirituality ; Spiritualität ; Trauma
Language	German
Document type	Article
ISSN	1863-7167
ISSN	1863-7167
DOI	10.21706/tg-16-3-218
Database	PSYNDEX

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Psychodrama als Verfahren innerhalb der religionspsychologischen Praxis. Exemplifiziert an der psychodramatischen Lesung des 'Zauberbergs'

Trettin, Alexander

cultura & psyché

2021 Volume 2, Issue 2, Page(s) 197–211

Abstract: Der Beitrag beschreibt, wie Psychodrama als Verfahren innerhalb der religionspsychologischen Praxis empirisch eingesetzt werden kann, um religiöse/spirituelle Strukturen sichtbar bzw. religiöse/spirituelle Phänomene begreifbar zu machen. Hierbei gilt es, ...

Title translation	Psychodrama as a process within the practice of the psychology of religion. Exemplified by the psychodramatic reading of the 'Magic Mountain'
Abstract	Der Beitrag beschreibt, wie Psychodrama als Verfahren innerhalb der religionspsychologischen Praxis empirisch eingesetzt werden kann, um religiöse/spirituelle Strukturen sichtbar bzw. religiöse/spirituelle Phänomene begreifbar zu machen. Hierbei gilt es, Religiosität bzw. Spiritualität als therapeutische Ressource herauszuarbeiten. Im Artikel werden zudem die religionspsychologischen Anteile des Psychodramas skizziert, die schließlich in Überlegungen zum Ritual und zur Katharsis münden. Die psychodramatische Lesung des 'Zauberbergs' von vier Freimaurern dient hierbei als Fallbeispiel für die praktische Anwendung.
Keywords	Literatur ; Literature ; Psychodrama ; Psychotherapeutic Processes ; Psychotherapeutic Techniques ; Psychotherapeutische Prozesse ; Psychotherapeutische Techniken ; Role Playing ; Rollenspiel ; Spirituality ; Spiritualität
Language	German
Document type	Article
DOI	10.1007/s43638-022-00037-z
Database	PSYNDEX

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article ; Online: Synthetic dyes: A mass spectrometry approach and applications.

Millbern, Zoe / Trettin, Alison / Wu, Rachel / Demmler, Morgan / Vinueza, Nelson R

Mass spectrometry reviews

2022 Volume 43, Issue 2, Page(s) 327–344

Abstract: Synthetic dyes are found in a wide variety of applications today, including but not limited to textiles, foods, and medicine. The analysis of these molecules is pertinent to several fields such as forensics, environmental monitoring, and quality control, ...

Abstract	Synthetic dyes are found in a wide variety of applications today, including but not limited to textiles, foods, and medicine. The analysis of these molecules is pertinent to several fields such as forensics, environmental monitoring, and quality control, all of which require the sensitivity and selectivity of analysis provided by mass spectrometry (MS). Recently, there has been an increase in the implementation of MS evaluation of synthetic dyes by various methods, with the majority of research thus far falling under electrospray ionization and moving toward direct ionization methods. This review covers an overview of the chemistry of synthetic dyes needed for the understanding of MS sample preparation and spectral results, current fields of application, ionization methods, and fragmentation trends and works that have been reported in recent years.
Language	English
Publishing date	2022-11-10
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	1491946-1
ISSN	1098-2787 ; 0277-7037
ISSN (online)	1098-2787
ISSN	0277-7037
DOI	10.1002/mas.21818
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Details ▾
- Full text online
- Order with fees

Article ; Online: LC-MS/MS analysis of uncommon paracetamol metabolites derived through in vitro polymerization and nitration reactions in liquid nitrogen.

Trettin, Arne / Jordan, Jens / Tsikas, Dimitrios

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

2014 Volume 966, Page(s) 171–178

Abstract: Paracetamol (acetaminophen, APAP) is a commonly used analgesic drug. Known paracetamol metabolites include the glucuronide, sulfate and mercapturate. N-Acetyl-benzoquinonimine (NAPQI) is considered the toxic intermediate metabolite of paracetamol. In ... ...

Abstract	Paracetamol (acetaminophen, APAP) is a commonly used analgesic drug. Known paracetamol metabolites include the glucuronide, sulfate and mercapturate. N-Acetyl-benzoquinonimine (NAPQI) is considered the toxic intermediate metabolite of paracetamol. In vitro and in vivo studies indicate that paracetamol is also metabolized to additional poorly characterized metabolites. For example, metabolomic studies in urine samples of APAP-treated mice revealed metabolites such as APAP-sulfate-APAP and APAP-S-S-APAP in addition to the classical phase II metabolites. Here, we report on the development and application of LC-MS and LC-MS/MS approaches to study reactions of unlabelled and (2)H-labelled APAP with unlabelled and (15)N-labelled nitrite in aqueous phosphate buffers (pH 7.4) upon their immersion into liquid nitrogen (-196°C). In mechanistic studies, these reactions were also studied in aqueous buffer prepared in (18)O-labelled water. LC-MS and LC-MS/MS analyses were performed on a reverse-phase material (C18) using gradient elution (2mM ammonium acetate/acetonitrile), in positive and negative electrospray mode. We identified a series of APAP metabolites including di-, tri- and tetra-APAP, mono- and di-nitro-APAP and nitric ester of di-APAP. Our study indicates that nitrite induces oxidation, i.e., polymerization and nitration of APAP, when buffered APAP/nitrite solutions are immersed into liquid nitrogen. These reactions are specific for nitrite with respect to nitrate and do not proceed via intermediate formation of NAPQI. Potassium ions and physiological saline but not thiols inhibit nitrite- and shock-freeze-induced reactions of paracetamol. The underlying mechanism likely involves in situ formation of NO2 radicals from nitrite secondary to profound pH reduction (down to pH 1) and disproportionation. Polymeric paracetamol species can be analyzed as pentafluorobenzyl derivatives by LC-MS but not by GC-MS.
MeSH term(s)	Acetaminophen/analogs & derivatives ; Acetaminophen/chemistry ; Animals ; Chromatography, Liquid/methods ; Humans ; Mice ; Nitric Oxide/chemistry ; Nitrogen/chemistry ; Polymerization ; Tandem Mass Spectrometry/methods
Chemical Substances	Nitric Oxide (31C4KY9ESH) ; Acetaminophen (362O9ITL9D) ; Nitrogen (N762921K75)
Language	English
Publishing date	2014-09-01
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1180823-8
ISSN	1873-376X ; 0378-4347 ; 1570-0232 ; 1387-2273
ISSN (online)	1873-376X
ISSN	0378-4347 ; 1570-0232 ; 1387-2273
DOI	10.1016/j.jchromb.2013.11.055
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 813: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: LCâMS/MS analysis of uncommon paracetamol metabolites derived through in vitro polymerization and nitration reactions in liquid nitrogen

Trettin, Arne / Dimitrios Tsikas / Jens Jordan

Journal of Chromatography B. 2014 Sept. 01, v. 966

2014

Abstract	Paracetamol (acetaminophen, APAP) is a commonly used analgesic drug. Known paracetamol metabolites include the glucuronide, sulfate and mercapturate. N-Acetyl-benzoquinonimine (NAPQI) is considered the toxic intermediate metabolite of paracetamol. In vitro and in vivo studies indicate that paracetamol is also metabolized to additional poorly characterized metabolites. For example, metabolomic studies in urine samples of APAP-treated mice revealed metabolites such as APAP-sulfate-APAP and APAP-S-S-APAP in addition to the classical phase II metabolites. Here, we report on the development and application of LCâMS and LCâMS/MS approaches to study reactions of unlabelled and 2H-labelled APAP with unlabelled and 15N-labelled nitrite in aqueous phosphate buffers (pH 7.4) upon their immersion into liquid nitrogen (â196Â°C). In mechanistic studies, these reactions were also studied in aqueous buffer prepared in 18O-labelled water. LCâMS and LCâMS/MS analyses were performed on a reverse-phase material (C18) using gradient elution (2mM ammonium acetate/acetonitrile), in positive and negative electrospray mode. We identified a series of APAP metabolites including di-, tri- and tetra-APAP, mono- and di-nitro-APAP and nitric ester of di-APAP. Our study indicates that nitrite induces oxidation, i.e., polymerization and nitration of APAP, when buffered APAP/nitrite solutions are immersed into liquid nitrogen. These reactions are specific for nitrite with respect to nitrate and do not proceed via intermediate formation of NAPQI. Potassium ions and physiological saline but not thiols inhibit nitrite- and shock-freeze-induced reactions of paracetamol. The underlying mechanism likely involves in situ formation of NO2 radicals from nitrite secondary to profound pH reduction (down to pH 1) and disproportionation. Polymeric paracetamol species can be analyzed as pentafluorobenzyl derivatives by LCâMS but not by GCâMS.
Keywords	acetaminophen ; acetonitrile ; ammonium acetate ; buffers ; cations ; free radicals ; gas chromatography-mass spectrometry ; in vivo studies ; liquid chromatography ; metabolites ; metabolomics ; mice ; nitrites ; nitrogen ; nitrogen dioxide ; oxidation ; pH ; phosphates ; polymerization ; potassium ; sulfates ; thiols ; toxicity ; urine
Language	English
Dates of publication	2014-0901
Size	p. 171-178.
Publishing place	Elsevier B.V.
Document type	Article
ISSN	1570-0232
DOI	10.1016/j.jchromb.2013.11.055
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Trapping of NAPQI, the intermediate toxic paracetamol metabolite, by aqueous sulfide (S²⁻) and analysis by GC-MS/MS.

Trettin, Arne / Batkai, Sandor / Thum, Thomas / Jordan, Jens / Tsikas, Dimitrios

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

2014 Volume 963, Page(s) 99–105

Abstract: NAPQI, i.e., N-acetyl-p-benzoquinone imine, is considered the toxic metabolite of the widely used analgesic drug paracetamol (acetaminophen, APAP). Due to its high reactivity towards nucleophiles both in low- and high-molecular-mass biomolecules, NAPQI ... ...

Abstract	NAPQI, i.e., N-acetyl-p-benzoquinone imine, is considered the toxic metabolite of the widely used analgesic drug paracetamol (acetaminophen, APAP). Due to its high reactivity towards nucleophiles both in low- and high-molecular-mass biomolecules, NAPQI is hardly detectable in its native form. Upon conjugation with glutathione, NAPQI is finally excreted in the urine as the paracetamol mercapturic acid. Thus, determination of paracetamol mercapturate may provide a measure of in vivo NAPQI formation. In this work, we propose the use of Na2S in aqueous solution to trap NAPQI and to analyze the reaction product, i.e., 3-thio-paracetamol, together with paracetamol by GC-MS/MS in the electron-capture negative-ion chemical ionization mode after solvent extraction with ethyl acetate and derivatization with pentafluorobenzyl bromide. In mechanistic studies, we used newly synthesized N-acetyl-p-[2,3,5,6-(2)H4]benzoquinone imine (d4-NAPQI). In quantitative analyses, N-(4-hydroxyphenyl)-[2,3,5,6-(2)H4]acetamide (d4-APAP) was used as the internal standard both for NAPQI and APAP. 3-Thio-d3-paracetamol, prepared from d4-NAPQI and Na2S, may also be useful as an internal standard. We showed NAPQI in vitro formation from APAP by recombinant cyclooxygenase-1 as well as by dog liver homogenate. In vivo formation of NAPQI was demonstrated in mice given paracetamol intraperitoneally (about 150 mg/kg).
MeSH term(s)	Acetaminophen/analogs & derivatives ; Acetaminophen/metabolism ; Analgesics, Non-Narcotic/chemistry ; Analgesics, Non-Narcotic/metabolism ; Animals ; Benzoquinones/analysis ; Benzoquinones/metabolism ; Cyclooxygenase 1/metabolism ; Dogs ; Gas Chromatography-Mass Spectrometry ; Imines/analysis ; Imines/metabolism ; Liver/metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Recombinant Proteins/metabolism ; Sulfides/chemistry ; Tandem Mass Spectrometry ; Water/chemistry
Chemical Substances	Analgesics, Non-Narcotic ; Benzoquinones ; Imines ; Recombinant Proteins ; Sulfides ; Water (059QF0KO0R) ; Acetaminophen (362O9ITL9D) ; Cyclooxygenase 1 (EC 1.14.99.1) ; N-acetyl-4-benzoquinoneimine (G6S9BN13TI) ; sodium sulfide (YGR27ZW0Y7)
Language	English
Publishing date	2014-07-15
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1180823-8
ISSN	1873-376X ; 0378-4347 ; 1570-0232 ; 1387-2273
ISSN (online)	1873-376X
ISSN	0378-4347 ; 1570-0232 ; 1387-2273
DOI	10.1016/j.jchromb.2014.05.050
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 813: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Trapping of NAPQI, the intermediate toxic paracetamol metabolite, by aqueous sulfide (S2â) and analysis by GCâMS/MS

Trettin, Arne / Dimitrios Tsikas / Jens Jordan / Sandor Batkai / Thomas Thum

Journal of Chromatography B. 2014 July 15, v. 963

2014

Abstract	NAPQI, i.e., N-acetyl-p-benzoquinone imine, is considered the toxic metabolite of the widely used analgesic drug paracetamol (acetaminophen, APAP). Due to its high reactivity towards nucleophiles both in low- and high-molecular-mass biomolecules, NAPQI is hardly detectable in its native form. Upon conjugation with glutathione, NAPQI is finally excreted in the urine as the paracetamol mercapturic acid. Thus, determination of paracetamol mercapturate may provide a measure of in vivo NAPQI formation. In this work, we propose the use of Na2S in aqueous solution to trap NAPQI and to analyze the reaction product, i.e., 3-thio-paracetamol, together with paracetamol by GCâMS/MS in the electron-capture negative-ion chemical ionization mode after solvent extraction with ethyl acetate and derivatization with pentafluorobenzyl bromide. In mechanistic studies, we used newly synthesized N-acetyl-p-[2,3,5,6-2H4]benzoquinone imine (d4-NAPQI). In quantitative analyses, N-(4-hydroxyphenyl)-[2,3,5,6-2H4]acetamide (d4-APAP) was used as the internal standard both for NAPQI and APAP. 3-Thio-d3-paracetamol, prepared from d4-NAPQI and Na2S, may also be useful as an internal standard. We showed NAPQI in vitro formation from APAP by recombinant cyclooxygenase-1 as well as by dog liver homogenate. In vivo formation of NAPQI was demonstrated in mice given paracetamol intraperitoneally (about 150mg/kg).
Keywords	acetaminophen ; aqueous solutions ; benzoquinones ; chromatography ; derivatization ; dogs ; ethyl acetate ; glutathione ; ionization ; liver ; metabolites ; mice ; prostaglandin synthase ; quantitative analysis ; solvents ; sulfides ; toxicity ; urine
Language	English
Dates of publication	2014-0715
Size	p. 99-105.
Publishing place	Elsevier B.V.
Document type	Article
ISSN	1570-0232
DOI	10.1016/j.jchromb.2014.05.050
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: GC-MS/MS and LC-MS/MS studies on unlabelled and deuterium-labelled oleic acid (C18:1) reactions with peroxynitrite (O=N-O-O⁻) in buffer and hemolysate support the pM/nM-range of nitro-oleic acids in human plasma.

Trettin, Arne / Böhmer, Anke / Zoerner, Alexander A / Gutzki, Frank-Mathias / Jordan, Jens / Tsikas, Dimitrios

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

2014 Volume 964, Page(s) 172–179

Abstract: Oleic acid (cis-9,10-octadecenoic acid) is the most abundant monounsaturated fatty acid in human blood. Peroxynitrite (ONOO(-)) is a short-lived species formed from the reaction of nitric oxide (NO) and superoxide (O2(-)). Peroxynitrite is a potent ... ...

Abstract	Oleic acid (cis-9,10-octadecenoic acid) is the most abundant monounsaturated fatty acid in human blood. Peroxynitrite (ONOO(-)) is a short-lived species formed from the reaction of nitric oxide (NO) and superoxide (O2(-)). Peroxynitrite is a potent oxidizing and moderate nitrating agent. We investigated reactions of unlabelled and deuterium labelled oleic acid in phosphate buffered saline (PBS) and lysed human erythrocytes with commercially available sodium peroxynitrite (Na(+)ONOO(-)). Non-derivatized reaction products were analyzed by spectrophotometry, HPLC with UV absorbance detection, and LC-MS/MS electrospray ionization in the negative-ion mode. Reaction products were also analyzed by GC-MS/MS in the electron capture negative-ion chemical ionization mode after derivatization first with pentafluorobenzyl (PFB) bromide and then with N,O-bis(trimethylsilyl)trifluoroacetamide. Identified oleic acid reaction products in PBS and hemolysate include cis-9,10-epoxystearic acid and trans-9,10-epoxystearic acid (about 0.1% with respect to oleic acid), threo- and erythro-9,10-dihydroxy-stearic acids. Vinyl nitro-oleic acids, 9-nitro-oleic acid (9-NO2OA) and 10-nitro-oleic acid (10-NO2OA), or other nitro-oleic acids were not found to be formed from the reaction of oleic acid with peroxynitrite in PBS or hemolysate. Our in vitro study suggests that peroxynitrite oxidizes but does not nitrate oleic acid in biological samples. Unlike thiols and tyrosine, oleic acid is not susceptible to peroxynitrite. GC-MS/MS analysis of PFB esters is by far more efficient than LC-MS/MS analysis of non-derivatized oleic acid and its derivates. Our in vitro results support our previous in vivo findings that nitro-oleic acid plasma concentrations of healthy and diseased subjects are in the pM/nM-range.
MeSH term(s)	Buffers ; Chromatography, High Pressure Liquid ; Deuterium/chemistry ; Erythrocytes/chemistry ; Gas Chromatography-Mass Spectrometry ; Hemolysis ; Humans ; Hydroxylation ; Nitro Compounds/blood ; Nitro Compounds/chemistry ; Oleic Acid/blood ; Oleic Acid/chemistry ; Oxidation-Reduction ; Peroxynitrous Acid/chemistry ; Tandem Mass Spectrometry
Chemical Substances	Buffers ; Nitro Compounds ; Peroxynitrous Acid (14691-52-2) ; Oleic Acid (2UMI9U37CP) ; Deuterium (AR09D82C7G)
Language	English
Publishing date	2014-08-01
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1180823-8
ISSN	1873-376X ; 0378-4347 ; 1570-0232 ; 1387-2273
ISSN (online)	1873-376X
ISSN	0378-4347 ; 1570-0232 ; 1387-2273
DOI	10.1016/j.jchromb.2014.01.016
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 813: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: In-source formation of N-acetyl-p-benzoquinone imine (NAPQI), the putatively toxic acetaminophen (paracetamol) metabolite, after derivatization with pentafluorobenzyl bromide and GC-ECNICI-MS analysis.

Tsikas, Dimitrios / Trettin, Arne / Zörner, Alexander A / Gutzki, Frank-Mathias

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

2011 Volume 879, Issue 17-18, Page(s) 1476–1484

Abstract: Pentafluorobenzyl (PFB) bromide (PFB-Br) is a versatile derivatization reagent for numerous classes of compounds. Under electron-capture negative-ion chemical ionization (ECNICI) conditions PFB derivatives of acidic compounds readily and abundantly ... ...

Abstract	Pentafluorobenzyl (PFB) bromide (PFB-Br) is a versatile derivatization reagent for numerous classes of compounds. Under electron-capture negative-ion chemical ionization (ECNICI) conditions PFB derivatives of acidic compounds readily and abundantly ionize to produce intense anions due to [M-PFB](-). In the present article we investigated the PFB-Br derivatization of unlabelled acetaminophen (N-acetyl-p-aminophenol, NAPAP-d(0); paracetamol; MW 151) and tetradeuterated acetaminophen (NAPAP-d(4); MW 155) in anhydrous acetonitrile and their GC-ECNICI-MS behavior using methane as the buffer gas. In addition to the expected anions [M-PFB](-) at m/z 150 from NAPAP-d(0) and m/z 154 from NAPAP-d(4), we observed highly reproducibly almost equally intense anions at m/z 149 and m/z 153, respectively. Selected ion monitoring of these ions is suitable for specific and sensitive quantification of acetaminophen in human plasma and urine. Detailed investigations suggest in-source formation of N-acetyl-p-benzoquinone imine (NAPQI; MW 149), the putatively toxic acetaminophen metabolite, from the PFB ether derivative of NAPAP. GC-ECNICI-MS of non-derivatized NAPAP did not produce NAPQI. The peak area ratio of m/z 149 to m/z 150 and of m/z 153 to m/z 154 decreased with increasing ion-source temperature in the range 100-250°C. Most likely, NAPQI formed in the ion-source captures secondary electrons to become negatively charged (i.e., [NAPQI](-)) and thus detectable. Formation of NAPQI was not observed under electron ionization (EI) conditions, i.e., by GC-EI-MS, from derivatized and non-derivatized NAPAP. NAPQI was not detectable in flow injection analysis LC-MS of native NAPAP in positive electrospray ionization (ESI) mode, whereas in negative ESI mode low extent NAPQI formation was observed (<5%). Our results suggest that oxidation of drug derivatives in the ion-sources of mass spectrometers may form intermediates that are produced from activated drugs in enzyme-catalyzed reactions.
MeSH term(s)	Acetaminophen/chemistry ; Acetonitriles ; Analgesics, Non-Narcotic/chemistry ; Benzoquinones/chemistry ; Fluorobenzenes/chemistry ; Gas Chromatography-Mass Spectrometry/methods ; Imines/chemistry ; Methane
Chemical Substances	Acetonitriles ; Analgesics, Non-Narcotic ; Benzoquinones ; Fluorobenzenes ; Imines ; pentafluorobenzyl bromide (1765-40-8) ; Acetaminophen (362O9ITL9D) ; N-acetyl-4-benzoquinoneimine (G6S9BN13TI) ; Methane (OP0UW79H66) ; acetonitrile (Z072SB282N)
Language	English
Publishing date	2011-05-15
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1180823-8
ISSN	1873-376X ; 0378-4347 ; 1570-0232 ; 1387-2273
ISSN (online)	1873-376X
ISSN	0378-4347 ; 1570-0232 ; 1387-2273
DOI	10.1016/j.jchromb.2011.01.025
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 813: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Development, validation and biomedical applications of stable-isotope dilution GC-MS and GC-MS/MS techniques for circulating malondialdehyde (MDA) after pentafluorobenzyl bromide derivatization: MDA as a biomarker of oxidative stress and its relation to 15(S)-8-iso-prostaglandin F2α and nitric oxide (NO).

Tsikas, Dimitrios / Rothmann, Sabine / Schneider, Jessica Y / Suchy, Maria-Theresia / Trettin, Arne / Modun, Darko / Stuke, Nadine / Maassen, Norbert / Frölich, Jürgen C

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

2016 Volume 1019, Page(s) 95–111

Abstract: Malondialdehyde (MDA, CH2(CHO)2) is one of the best investigated and most frequently measured biomarkers of lipid peroxidation in biological fluids, a constituent of the so called thiobarbituric acid reactive substances (TBARS). The reaction of ... ...

Abstract	Malondialdehyde (MDA, CH2(CHO)2) is one of the best investigated and most frequently measured biomarkers of lipid peroxidation in biological fluids, a constituent of the so called thiobarbituric acid reactive substances (TBARS). The reaction of thiobarbituric acid with MDA and other carbonyl compounds is the basis for the batch TBARS assay, one of the most commonly and widely used assays of oxidative stress. Yet, the TBARS assay lacks specificity even if combined with HPLC separation prior to visible absorbance or fluorescence detection. In this article, we report highly specific and sensitive stable-isotope dilution GC-MS and GC-MS/MS methods for the quantitative determination of MDA in human plasma (0.1 mL). These methods utilize the acidity (pKa, 4.46) of the two methylene H protons of MDA in aqueous solution, which are as acidic as acetic acid. Endogenous MDA in native plasma and the externally added internal standard [1,3-(2)H2]-MDA (d2-MDA, CH2(CDO)2) are derivatized in aqueous acetone (400 μL) with pentafluorobenzyl (PFB) bromide (10 μL). The reaction products were identified as C(PFB)2(CHO)2 (molecular weight, 432) and C(PFB)2(CDO)2) (molecular weight, 434), respectively. After solvent extraction with toluene (1 mL) quantification is performed by selected-ion monitoring (SIM) in GC-MS and by selected-reaction monitoring (SRM) in GC-MS/MS in the electron-capture negative-ion chemical ionization (ECNICI) mode. In the SIM mode, the anions [M-PFB](-) at m/z 251 for MDA and m/z 253 for d2-MDA are detected. In the SRM mode, the mass transitions m/z 251 to m/z 175 for MDA and m/z 253 to m/z 177 for d2-MDA are monitored. The method was thoroughly validated in human plasma. Potential interfering substances including anticoagulants and commercially available monovettes commonly used for blood sampling were tested. The lowest MDA concentrations were measured in serum followed by heparinized and EDTA plasma. The GC-MS and GC-MS/MS methods were found to be specific, precise, accurate and sensitive. Thus, the LOD of the GC-MS/MS method was determined to be 2 amol (2 × 10(-18)mol) MDA. The GC-MS/MS method is exceedingly useful in clinical settings. We report several biomedical applications and discuss the utility of circulating MDA as a biomarker of lipid peroxidation, especially in long-term clinical studies, and its relation to the F2-isoprostane 15(S)-8-iso-prostaglandin F2α and nitric oxide (NO).
MeSH term(s)	Biomarkers/blood ; Biomarkers/urine ; Deuterium/chemistry ; Dinoprost/metabolism ; Fluorobenzenes/chemistry ; Gas Chromatography-Mass Spectrometry/methods ; Humans ; Indicator Dilution Techniques ; Malondialdehyde/blood ; Malondialdehyde/chemistry ; Malondialdehyde/urine ; Nitric Oxide/metabolism ; Oxidative Stress ; Tandem Mass Spectrometry/methods
Chemical Substances	Biomarkers ; Fluorobenzenes ; pentafluorobenzyl bromide (1765-40-8) ; Nitric Oxide (31C4KY9ESH) ; Malondialdehyde (4Y8F71G49Q) ; Deuterium (AR09D82C7G) ; Dinoprost (B7IN85G1HY)
Language	English
Publishing date	2016-04-15
Publishing country	Netherlands
Document type	Evaluation Studies ; Journal Article
ZDB-ID	1180823-8
ISSN	1873-376X ; 0378-4347 ; 1570-0232 ; 1387-2273
ISSN (online)	1873-376X
ISSN	0378-4347 ; 1570-0232 ; 1387-2273
DOI	10.1016/j.jchromb.2015.10.009
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 813: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito